Click here to switch to the map view.

The map label for this gene is cysH [H]

Identifier: 222524032

GI number: 222524032

Start: 931872

End: 932537

Strand: Reverse

Name: cysH [H]

Synonym: Chy400_0747

Alternate gene names: 222524032

Gene position: 932537-931872 (Counterclockwise)

Preceding gene: 222524033

Following gene: 222524031

Centisome position: 17.7

GC content: 57.96

Gene sequence:

>666_bases
ATGACAACACAATACTGGGACGCGAACACCGACCCTCGTGATGTCATTCGCTGGGCACTTGCCGGTTATCCCGATCTGCT
GATGACCAGTGCATTCAATCTTAACGGGGTCGTGCTTATTGAGCTGGCAGCTCAGGCCGGTTTTGCGGGCGAGGTGGTTT
TTGTGGATACCGGCTACCATTTTCCGGAGACCCTTGCCACTCGTGACCATCTGATCACGCGCTATCCCCAGATGCAGTTT
GTCACCCTAAGTGCCGACCTGCCACCTGAACCGTGGGGGGAAGAGCAATTTCGGAGCGATCCCGATGGGTGCTGTCGCGT
GCGAAAAGTTGCTCCCCTGCGTGAATATCTGGCGCGCAAGAATCCTTCTGCTCTTTTAAACGCTCGCAGTCGTGATCAGG
CCGCAACCCGCCGGGATCTGGGTTTTGTCGAGATTGGTGAGCGGGTGCGGATCAATCCACTGGCCTACTGGCGGCGGGAG
CAGATAGAGGCATTTGCGCAAGCCCATCACCTGCCGGTGAACCCATTGTACTGGGCCGGTTTCAAGAGTATCGGCTGCTG
GCCCTGTACACGGGCCGTGCGCCCTGGTGAAGATGTGCGGGCCGGGCGTTGGTCGGGGAAGGGCAAGATTGAATGTGGCC
TATGGATCGGTGAACAATCGCTGTGA

Upstream 100 bases:

>100_bases
ATGTCCATCTGCGCACCGATCTGATGAGTTTGCCGGAGTGTCTGGATCATCTGCTGGCTGCGCTGGAACGGCTGGATATT
GTGGTGAAGGTGAGTCACCA

Downstream 100 bases:

>100_bases
ACGAGGTGAGGTGATGATACCACAAACGAGTTCTCTCCCGAAGCCGCACGGTGGTGTTCTGGTTGAGCGGATTCGGGTTG
CACATCCACGCGAATATGAC

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTTQYWDANTDPRDVIRWALAGYPDLLMTSAFNLNGVVLIELAAQAGFAGEVVFVDTGYHFPETLATRDHLITRYPQMQF
VTLSADLPPEPWGEEQFRSDPDGCCRVRKVAPLREYLARKNPSALLNARSRDQAATRRDLGFVEIGERVRINPLAYWRRE
QIEAFAQAHHLPVNPLYWAGFKSIGCWPCTRAVRPGEDVRAGRWSGKGKIECGLWIGEQSL

Sequences:

>Translated_221_residues
MTTQYWDANTDPRDVIRWALAGYPDLLMTSAFNLNGVVLIELAAQAGFAGEVVFVDTGYHFPETLATRDHLITRYPQMQF
VTLSADLPPEPWGEEQFRSDPDGCCRVRKVAPLREYLARKNPSALLNARSRDQAATRRDLGFVEIGERVRINPLAYWRRE
QIEAFAQAHHLPVNPLYWAGFKSIGCWPCTRAVRPGEDVRAGRWSGKGKIECGLWIGEQSL
>Mature_220_residues
TTQYWDANTDPRDVIRWALAGYPDLLMTSAFNLNGVVLIELAAQAGFAGEVVFVDTGYHFPETLATRDHLITRYPQMQFV
TLSADLPPEPWGEEQFRSDPDGCCRVRKVAPLREYLARKNPSALLNARSRDQAATRRDLGFVEIGERVRINPLAYWRREQ
IEAFAQAHHLPVNPLYWAGFKSIGCWPCTRAVRPGEDVRAGRWSGKGKIECGLWIGEQSL

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=214, Percent_Identity=28.5046728971963, Blast_Score=71, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325425, Length=218, Percent_Identity=33.4862385321101, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 25034; Mature: 24902

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQYWDANTDPRDVIRWALAGYPDLLMTSAFNLNGVVLIELAAQAGFAGEVVFVDTGYH
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCC
FPETLATRDHLITRYPQMQFVTLSADLPPEPWGEEQFRSDPDGCCRVRKVAPLREYLARK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCC
NPSALLNARSRDQAATRRDLGFVEIGERVRINPLAYWRREQIEAFAQAHHLPVNPLYWAG
CCHHHHCCCCHHHHHHHHCCCCEECCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCEECC
FKSIGCWPCTRAVRPGEDVRAGRWSGKGKIECGLWIGEQSL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
TTQYWDANTDPRDVIRWALAGYPDLLMTSAFNLNGVVLIELAAQAGFAGEVVFVDTGYH
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCC
FPETLATRDHLITRYPQMQFVTLSADLPPEPWGEEQFRSDPDGCCRVRKVAPLREYLARK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCC
NPSALLNARSRDQAATRRDLGFVEIGERVRINPLAYWRREQIEAFAQAHHLPVNPLYWAG
CCHHHHCCCCHHHHHHHHCCCCEECCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCEECC
FKSIGCWPCTRAVRPGEDVRAGRWSGKGKIECGLWIGEQSL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]