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The map label for this gene is phoE [H]

Identifier: 222523674

GI number: 222523674

Start: 447144

End: 447821

Strand: Reverse

Name: phoE [H]

Synonym: Chy400_0380

Alternate gene names: 222523674

Gene position: 447821-447144 (Counterclockwise)

Preceding gene: 222523676

Following gene: 222523673

Centisome position: 8.5

GC content: 56.34

Gene sequence:

>678_bases
ATGACAATTGTTTACCTGATCCGTCACGGTGAAACCGATTGGAATATGCAGGGACGCTGGCAGGGACACGCTGATGTTCC
GCTCAATGAGATTGGTTATCAGCAGGCACGCTTGCTGGCGCGGCGGCTGGCCAATGAAGGTGTGACGTTTAGCGCTATTT
ACAGCAGTGATCTCGCCAGAGCGTATCAGACGGCGTGGGAGATAGGCGCAGCCTTGCGCGTACCGGTGCAGCTCTTGCCG
GCATTGCGCGAAATTGATGTTGGGGCCTGGAGCGGGTTAACGACGGCAGAGGTGCGTGCCCGCTTTGCTGATGATTGGGA
GCAGATGATCCGGGGCCACGACATCCCACGCGGTGGTGGAGAAACGGTGGCCGCCGTCCGGCAGCGGGTCGTTACAGCCG
TTGAGGCAATGGTGGCGCAGCATCGTGGGCAGACACTGGCATTTGTGACGCACGGGGGTTGTATTCGGGTCTTGCTTGCC
CATGCCGAAAATTTTGATGGTGTCGGCTTTGGTCACTACGATCATATTGGCAATACTTCCATCACCATCCTGGAAATCGG
CTATAAGCACTGGCGGGTGAAGACGGTTAATGATATGCGCCATTTAGAGGCAGAACACAAGACTGAACTGGTTTCGGCAC
CACCCGACGATGCTGAGTTACCCGATACAGCGCAATGA

Upstream 100 bases:

>100_bases
ATGGTTGCAACTGCTACAGGTAGGGGAAACTGTTCATAATCCTGGTTATTGGTATGTGTTACCATAGTAACAGGTGTGGA
GATTGTTCACGAGGAAGAGC

Downstream 100 bases:

>100_bases
ACGCGACACCCTCATGGCGTGAGCCGTATCTGGTGGTGACACCAGAAGGTGTGCAGGTCGTCTACACGGCTGCCGGATTG
GCCAGCCGTAGTCTGGCAGC

Product: phosphoglycerate mutase

Products: 3-phospho-D-glycerate

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTIVYLIRHGETDWNMQGRWQGHADVPLNEIGYQQARLLARRLANEGVTFSAIYSSDLARAYQTAWEIGAALRVPVQLLP
ALREIDVGAWSGLTTAEVRARFADDWEQMIRGHDIPRGGGETVAAVRQRVVTAVEAMVAQHRGQTLAFVTHGGCIRVLLA
HAENFDGVGFGHYDHIGNTSITILEIGYKHWRVKTVNDMRHLEAEHKTELVSAPPDDAELPDTAQ

Sequences:

>Translated_225_residues
MTIVYLIRHGETDWNMQGRWQGHADVPLNEIGYQQARLLARRLANEGVTFSAIYSSDLARAYQTAWEIGAALRVPVQLLP
ALREIDVGAWSGLTTAEVRARFADDWEQMIRGHDIPRGGGETVAAVRQRVVTAVEAMVAQHRGQTLAFVTHGGCIRVLLA
HAENFDGVGFGHYDHIGNTSITILEIGYKHWRVKTVNDMRHLEAEHKTELVSAPPDDAELPDTAQ
>Mature_224_residues
TIVYLIRHGETDWNMQGRWQGHADVPLNEIGYQQARLLARRLANEGVTFSAIYSSDLARAYQTAWEIGAALRVPVQLLPA
LREIDVGAWSGLTTAEVRARFADDWEQMIRGHDIPRGGGETVAAVRQRVVTAVEAMVAQHRGQTLAFVTHGGCIRVLLAH
AENFDGVGFGHYDHIGNTSITILEIGYKHWRVKTVNDMRHLEAEHKTELVSAPPDDAELPDTAQ

Specific function: Phosphatase with broad substrate specificity. Does not have phosphoglycerate mutase activtiy [H]

COG id: COG0406

COG function: function code G; Fructose-2,6-bisphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate mutase family. GpmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI64762445, Length=159, Percent_Identity=30.8176100628931, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI64762406, Length=161, Percent_Identity=31.055900621118, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1786857, Length=164, Percent_Identity=33.5365853658537, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790856, Length=226, Percent_Identity=30.9734513274336, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786970, Length=197, Percent_Identity=28.4263959390863, Blast_Score=71, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324857, Length=209, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013078
- InterPro:   IPR001345 [H]

Pfam domain/function: PF00300 PGAM [H]

EC number: 5.4.2.1

Molecular weight: Translated: 25012; Mature: 24880

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00175 PG_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVYLIRHGETDWNMQGRWQGHADVPLNEIGYQQARLLARRLANEGVTFSAIYSSDLAR
CEEEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
AYQTAWEIGAALRVPVQLLPALREIDVGAWSGLTTAEVRARFADDWEQMIRGHDIPRGGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
ETVAAVRQRVVTAVEAMVAQHRGQTLAFVTHGGCIRVLLAHAENFDGVGFGHYDHIGNTS
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
ITILEIGYKHWRVKTVNDMRHLEAEHKTELVSAPPDDAELPDTAQ
EEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIVYLIRHGETDWNMQGRWQGHADVPLNEIGYQQARLLARRLANEGVTFSAIYSSDLAR
EEEEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
AYQTAWEIGAALRVPVQLLPALREIDVGAWSGLTTAEVRARFADDWEQMIRGHDIPRGGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
ETVAAVRQRVVTAVEAMVAQHRGQTLAFVTHGGCIRVLLAHAENFDGVGFGHYDHIGNTS
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
ITILEIGYKHWRVKTVNDMRHLEAEHKTELVSAPPDDAELPDTAQ
EEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-phospho-D-glycerate

Specific reaction: 2-phospho-D-glycerate = 3-phospho-D-glycerate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]