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The map label for this gene is malE [H]

Identifier: 222523591

GI number: 222523591

Start: 359242

End: 360522

Strand: Reverse

Name: malE [H]

Synonym: Chy400_0297

Alternate gene names: 222523591

Gene position: 360522-359242 (Counterclockwise)

Preceding gene: 222523595

Following gene: 222523590

Centisome position: 6.84

GC content: 57.46

Gene sequence:

>1281_bases
GTGCACCATCAGTCCTGGTTCTATCGTCTACTACTGCTTGGCGCATTGATGCTGACTGCCTGTTCCGTGCTTCCGTCTAC
TGCGCCGGTAAGGACATCCGAGCGCGTAGTCCTGCGTCTGTGGCACGCCTGGCCGGCAGCCGATAGCCGTGTCTTACAAT
CGCTGGTGGATCAGTTTAACCAGACCCATCCTGAATCGCAGATTGTGATCCAATCCCGTCCGGCAGTGTCACTCCCCTCC
GATGTGGCAACCACTATCAGCGAAGGTGGCGGTCCGCACCTGGTCATTGTGCAGAGTCACATGCTCGGTATGCTCGTCCA
GGCCGGCGTGTTGCGTTCGCTCGACGATATGCTCTCAAACAGTGAGGTAAATAGTCTGCTACCCGCTGCGGTTGGGGCTG
CTCAGGTCTCCGTCTCTGGTCAGCCCGTTCTGTTTGGGGTGCCAATTACGTTTGACACCCTCGCCCTCTACTACAACCGG
GCCAACGTGTTGCAACCACCGTCCAGTACCGAAGAAATGCTCCTCACCGCCCGTGCACTCACGAACCGTAATCAGACACC
ACCAGTCTGGGGATTGGCCTACAATCTGTCACTGGATCGAACCATTGGCTATCTCTACGCCTTCGGCGGCAGGGTATTTG
ATGAAAATGGCGCTGTTGTCCTGGGAAGCAGTGGTCGCGCCGGTACCGAACGCTGGTTAACCTGGTTAAGCCAGCTTTAC
CGCGATGAACAGTTGCTGGCGACGCTCGACGGTGTGCTGGTTGATCGAGCATTACAGGCCCGCGAAGCCATCATGACTAT
CGATTGGGCACACGCTCAGGCTGAGTACCGGGCAATCTGGAATGATCAACTTGGGGTAACACCGCTGCCGGTCTTGAGTG
CCGAAGATCGAGTTCCTCAACCGTATGTACAGGCTGATGTGATCGCACTCAATGCTCGCCTTGATAATCAGGCTGAGCAA
GCGGCTGCCCAGGCCTTCATTCGCTTTATGATCGATGTCAATAGCCAGCGGGCACTGCTGGCGGCTGGCCGCCAGCCTAC
CCAACTTGCACTCCAGCTCAGTGATAACGACCTTGCCGATAGCGTCCAACTGGCCGCCGCCCGTGCCTTTCGCCTTCAAG
CGCAGAACGGTCTGCCAATGCCCGCTGATCGCGTTGCGCACGAGATCGTCTGGACAACCCTGGTCGAGATGCAGCTCAAT
GCTGTGCGCGGCTTACTGACACCTGAACAGGCTGTGACACTGGCCGATGAATTCCTGCGTAGTCGATTGGCTTCACCCTG
A

Upstream 100 bases:

>100_bases
AGCACTGGTTGGGAGCCACGGGGCATCTTCATCTATGCCCCGGCTACATGTGATGCATCCTGCTATAATGAAGAAGCCCG
ACTGATACAGCGAGGAAGTT

Downstream 100 bases:

>100_bases
GATGGCGTGCGTCCAAAAGCGCTAATACGGCCCAATCCACGGAAGCTATCGCATCATCGAAAAGGTAGACACGGAGTAAT
GACGCGCCGGATGGGAAACC

Product: family 1 extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MHHQSWFYRLLLLGALMLTACSVLPSTAPVRTSERVVLRLWHAWPAADSRVLQSLVDQFNQTHPESQIVIQSRPAVSLPS
DVATTISEGGGPHLVIVQSHMLGMLVQAGVLRSLDDMLSNSEVNSLLPAAVGAAQVSVSGQPVLFGVPITFDTLALYYNR
ANVLQPPSSTEEMLLTARALTNRNQTPPVWGLAYNLSLDRTIGYLYAFGGRVFDENGAVVLGSSGRAGTERWLTWLSQLY
RDEQLLATLDGVLVDRALQAREAIMTIDWAHAQAEYRAIWNDQLGVTPLPVLSAEDRVPQPYVQADVIALNARLDNQAEQ
AAAQAFIRFMIDVNSQRALLAAGRQPTQLALQLSDNDLADSVQLAAARAFRLQAQNGLPMPADRVAHEIVWTTLVEMQLN
AVRGLLTPEQAVTLADEFLRSRLASP

Sequences:

>Translated_426_residues
MHHQSWFYRLLLLGALMLTACSVLPSTAPVRTSERVVLRLWHAWPAADSRVLQSLVDQFNQTHPESQIVIQSRPAVSLPS
DVATTISEGGGPHLVIVQSHMLGMLVQAGVLRSLDDMLSNSEVNSLLPAAVGAAQVSVSGQPVLFGVPITFDTLALYYNR
ANVLQPPSSTEEMLLTARALTNRNQTPPVWGLAYNLSLDRTIGYLYAFGGRVFDENGAVVLGSSGRAGTERWLTWLSQLY
RDEQLLATLDGVLVDRALQAREAIMTIDWAHAQAEYRAIWNDQLGVTPLPVLSAEDRVPQPYVQADVIALNARLDNQAEQ
AAAQAFIRFMIDVNSQRALLAAGRQPTQLALQLSDNDLADSVQLAAARAFRLQAQNGLPMPADRVAHEIVWTTLVEMQLN
AVRGLLTPEQAVTLADEFLRSRLASP
>Mature_426_residues
MHHQSWFYRLLLLGALMLTACSVLPSTAPVRTSERVVLRLWHAWPAADSRVLQSLVDQFNQTHPESQIVIQSRPAVSLPS
DVATTISEGGGPHLVIVQSHMLGMLVQAGVLRSLDDMLSNSEVNSLLPAAVGAAQVSVSGQPVLFGVPITFDTLALYYNR
ANVLQPPSSTEEMLLTARALTNRNQTPPVWGLAYNLSLDRTIGYLYAFGGRVFDENGAVVLGSSGRAGTERWLTWLSQLY
RDEQLLATLDGVLVDRALQAREAIMTIDWAHAQAEYRAIWNDQLGVTPLPVLSAEDRVPQPYVQADVIALNARLDNQAEQ
AAAQAFIRFMIDVNSQRALLAAGRQPTQLALQLSDNDLADSVQLAAARAFRLQAQNGLPMPADRVAHEIVWTTLVEMQLN
AVRGLLTPEQAVTLADEFLRSRLASP

Specific function: Involved in an abc transport system for maltotriose. Binds maltotriose much more tightly than maltose [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 46607; Mature: 46607

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHQSWFYRLLLLGALMLTACSVLPSTAPVRTSERVVLRLWHAWPAADSRVLQSLVDQFN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
QTHPESQIVIQSRPAVSLPSDVATTISEGGGPHLVIVQSHMLGMLVQAGVLRSLDDMLSN
CCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC
SEVNSLLPAAVGAAQVSVSGQPVLFGVPITFDTLALYYNRANVLQPPSSTEEMLLTARAL
CHHHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TNRNQTPPVWGLAYNLSLDRTIGYLYAFGGRVFDENGAVVLGSSGRAGTERWLTWLSQLY
HCCCCCCCEEEEEEECCHHHHHHHHHHHCCEEECCCCCEEECCCCCCCHHHHHHHHHHHH
RDEQLLATLDGVLVDRALQAREAIMTIDWAHAQAEYRAIWNDQLGVTPLPVLSAEDRVPQ
CCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PYVQADVIALNARLDNQAEQAAAQAFIRFMIDVNSQRALLAAGRQPTQLALQLSDNDLAD
CCHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCHHH
SVQLAAARAFRLQAQNGLPMPADRVAHEIVWTTLVEMQLNAVRGLLTPEQAVTLADEFLR
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SRLASP
HHCCCC
>Mature Secondary Structure
MHHQSWFYRLLLLGALMLTACSVLPSTAPVRTSERVVLRLWHAWPAADSRVLQSLVDQFN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
QTHPESQIVIQSRPAVSLPSDVATTISEGGGPHLVIVQSHMLGMLVQAGVLRSLDDMLSN
CCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC
SEVNSLLPAAVGAAQVSVSGQPVLFGVPITFDTLALYYNRANVLQPPSSTEEMLLTARAL
CHHHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TNRNQTPPVWGLAYNLSLDRTIGYLYAFGGRVFDENGAVVLGSSGRAGTERWLTWLSQLY
HCCCCCCCEEEEEEECCHHHHHHHHHHHCCEEECCCCCEEECCCCCCCHHHHHHHHHHHH
RDEQLLATLDGVLVDRALQAREAIMTIDWAHAQAEYRAIWNDQLGVTPLPVLSAEDRVPQ
CCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PYVQADVIALNARLDNQAEQAAAQAFIRFMIDVNSQRALLAAGRQPTQLALQLSDNDLAD
CCHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCHHH
SVQLAAARAFRLQAQNGLPMPADRVAHEIVWTTLVEMQLNAVRGLLTPEQAVTLADEFLR
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SRLASP
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11162100 [H]