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The map label for this gene is rho [H]

Identifier: 222523586

GI number: 222523586

Start: 354118

End: 355452

Strand: Direct

Name: rho [H]

Synonym: Chy400_0292

Alternate gene names: 222523586

Gene position: 354118-355452 (Clockwise)

Preceding gene: 222523585

Following gene: 222523587

Centisome position: 6.72

GC content: 50.64

Gene sequence:

>1335_bases
ATGACAGTTGCTGAGCTTGAAACAAAAACTCTCGCCGATTTACGCGAAATGGCGCGTAAACTTGACATTTCAGGGATCAG
CTCATTGAAAAAACGCGAGCTGATCGATAAACTAATTCAAGCGCAAACGGCAACGCAAACGCAGACGATAACAGTTACCG
AGCCTCCAGCGTTACCAGATCTCAATCCGATAGAAACAACAACCGTTGACCGAACTTCCATTGAGTTCAGCACGATATAC
AGTGATGGCATCCTGGACATTATGGCAGAAGGTTTCGGTTTCTTACGAGGTAATCGGATGCTGCCAAGTCCAGAAGATGT
CTATGTCTCACAATCGCAGATTCGTCGCTTTGCCCTACGCAGTGGAGATCGGATTTGGGGGCAAATTCGTCCGCCGAAAG
AGAGTGAGCGCTATTATTCATTATTGCGGGTTGAAAAGATTAATGATCAGGATCCGGAAACTGCCCGCAAACGACCATTT
TTTGATCAACTGACACCCATTTTTCCCAACGAACAGATTAAGTTAGAAACTGAACCAAATCTCTTGCATACCCGTCTGGT
CGATTTGATTGCCCCAATCGGTCGTGGTCAGCGAGGCTTAATCGTTTCTCCTCCCAAAGCGGGAAAGACGATGCTGTTGA
AGGCAATTGCCAACGGCATCACGACCAACTATCCTGACATTATTCTGATGGTCTTGTTGATTGGTGAGCGCCCGGAAGAA
GTCACCGATATGCGCCGTTCTGTGCGCGGCGAGGTGATCTCTTCGACGTTTGATGAGCCGGTCGAAAATCACACCAAAGT
GGCCGAGATGACGCTGGAGCGGGCGAAACGCCTGGTAGAAATTGGACATGATGTGGTCATTTTGATGGACTCGATCACCC
GACTGGCCAGGGCGTACAACGTTGCCATGCCCCCGAGTGGACGTACCCTGTCTGGTGGTATCGACCCGGTTGCCCTGTAT
CCGCCAAAACGCTTTTTCGGTGCTGCCCGTAACATTGAAAATGGTGGCTCGCTAACGATTATCGCCACCTGTCTAATCGA
TACCGGCTCACGCATGGACGATGTGATCTACGAAGAGTTCAAGGGCACCGGCAACATGGAACTCCACCTTGACCGGAAAC
TGGCCGAAAAACGCATCTTCCCGGCGATTGATATTCAGCGCTCCGGTACGCGCCGCGAGGATCTCCTGCTCAGCCCTGAC
ACGTTACGCCAGGTATGGACGTTGCGGCGAATGGTGAGCATGCTCGGTGAGAATGAAGGTACCGAACTGATGCTGACCCG
GATGGCGAAAACCAAATCGAATGCCGAGTTTTTACAGACCCTTAGTAAGAGTTAG

Upstream 100 bases:

>100_bases
TCTGTCGCCCCTGAGATACCTACACGACAGATGAAAACCATTCACTGCCAGATACAGTGTCTACTTTCTATCTCATTGAC
TTCAAGGAGCATGTATCGTG

Downstream 100 bases:

>100_bases
GCTGATGCAACTTTGGTTCACTGATATACGTACAGTATTGCGCCTGGTATGATACATCTGGTAACAGGAGGTCTCGGTTA
TGAATTGTCCACAGTGCGGC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTVAELETKTLADLREMARKLDISGISSLKKRELIDKLIQAQTATQTQTITVTEPPALPDLNPIETTTVDRTSIEFSTIY
SDGILDIMAEGFGFLRGNRMLPSPEDVYVSQSQIRRFALRSGDRIWGQIRPPKESERYYSLLRVEKINDQDPETARKRPF
FDQLTPIFPNEQIKLETEPNLLHTRLVDLIAPIGRGQRGLIVSPPKAGKTMLLKAIANGITTNYPDIILMVLLIGERPEE
VTDMRRSVRGEVISSTFDEPVENHTKVAEMTLERAKRLVEIGHDVVILMDSITRLARAYNVAMPPSGRTLSGGIDPVALY
PPKRFFGAARNIENGGSLTIIATCLIDTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAIDIQRSGTRREDLLLSPD
TLRQVWTLRRMVSMLGENEGTELMLTRMAKTKSNAEFLQTLSKS

Sequences:

>Translated_444_residues
MTVAELETKTLADLREMARKLDISGISSLKKRELIDKLIQAQTATQTQTITVTEPPALPDLNPIETTTVDRTSIEFSTIY
SDGILDIMAEGFGFLRGNRMLPSPEDVYVSQSQIRRFALRSGDRIWGQIRPPKESERYYSLLRVEKINDQDPETARKRPF
FDQLTPIFPNEQIKLETEPNLLHTRLVDLIAPIGRGQRGLIVSPPKAGKTMLLKAIANGITTNYPDIILMVLLIGERPEE
VTDMRRSVRGEVISSTFDEPVENHTKVAEMTLERAKRLVEIGHDVVILMDSITRLARAYNVAMPPSGRTLSGGIDPVALY
PPKRFFGAARNIENGGSLTIIATCLIDTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAIDIQRSGTRREDLLLSPD
TLRQVWTLRRMVSMLGENEGTELMLTRMAKTKSNAEFLQTLSKS
>Mature_443_residues
TVAELETKTLADLREMARKLDISGISSLKKRELIDKLIQAQTATQTQTITVTEPPALPDLNPIETTTVDRTSIEFSTIYS
DGILDIMAEGFGFLRGNRMLPSPEDVYVSQSQIRRFALRSGDRIWGQIRPPKESERYYSLLRVEKINDQDPETARKRPFF
DQLTPIFPNEQIKLETEPNLLHTRLVDLIAPIGRGQRGLIVSPPKAGKTMLLKAIANGITTNYPDIILMVLLIGERPEEV
TDMRRSVRGEVISSTFDEPVENHTKVAEMTLERAKRLVEIGHDVVILMDSITRLARAYNVAMPPSGRTLSGGIDPVALYP
PKRFFGAARNIENGGSLTIIATCLIDTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAIDIQRSGTRREDLLLSPDT
LRQVWTLRRMVSMLGENEGTELMLTRMAKTKSNAEFLQTLSKS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=373, Percent_Identity=59.5174262734584, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 49952; Mature: 49821

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAELETKTLADLREMARKLDISGISSLKKRELIDKLIQAQTATQTQTITVTEPPALPD
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
LNPIETTTVDRTSIEFSTIYSDGILDIMAEGFGFLRGNRMLPSPEDVYVSQSQIRRFALR
CCCCCCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHH
SGDRIWGQIRPPKESERYYSLLRVEKINDQDPETARKRPFFDQLTPIFPNEQIKLETEPN
CCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHCCCCCCCCCEEEECCCC
LLHTRLVDLIAPIGRGQRGLIVSPPKAGKTMLLKAIANGITTNYPDIILMVLLIGERPEE
HHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHH
VTDMRRSVRGEVISSTFDEPVENHTKVAEMTLERAKRLVEIGHDVVILMDSITRLARAYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHC
VAMPPSGRTLSGGIDPVALYPPKRFFGAARNIENGGSLTIIATCLIDTGSRMDDVIYEEF
CCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
KGTGNMELHLDRKLAEKRIFPAIDIQRSGTRREDLLLSPDTLRQVWTLRRMVSMLGENEG
CCCCCEEEEECHHHHHHHCCCCEECCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCC
TELMLTRMAKTKSNAEFLQTLSKS
CHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
TVAELETKTLADLREMARKLDISGISSLKKRELIDKLIQAQTATQTQTITVTEPPALPD
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
LNPIETTTVDRTSIEFSTIYSDGILDIMAEGFGFLRGNRMLPSPEDVYVSQSQIRRFALR
CCCCCCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHH
SGDRIWGQIRPPKESERYYSLLRVEKINDQDPETARKRPFFDQLTPIFPNEQIKLETEPN
CCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHCCCCCCCCCEEEECCCC
LLHTRLVDLIAPIGRGQRGLIVSPPKAGKTMLLKAIANGITTNYPDIILMVLLIGERPEE
HHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHH
VTDMRRSVRGEVISSTFDEPVENHTKVAEMTLERAKRLVEIGHDVVILMDSITRLARAYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHC
VAMPPSGRTLSGGIDPVALYPPKRFFGAARNIENGGSLTIIATCLIDTGSRMDDVIYEEF
CCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
KGTGNMELHLDRKLAEKRIFPAIDIQRSGTRREDLLLSPDTLRQVWTLRRMVSMLGENEG
CCCCCEEEEECHHHHHHHCCCCEECCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCC
TELMLTRMAKTKSNAEFLQTLSKS
CHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]