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The map label for this gene is dus [H]

Identifier: 222523518

GI number: 222523518

Start: 267331

End: 268383

Strand: Direct

Name: dus [H]

Synonym: Chy400_0224

Alternate gene names: 222523518

Gene position: 267331-268383 (Clockwise)

Preceding gene: 222523517

Following gene: 222523519

Centisome position: 5.07

GC content: 59.54

Gene sequence:

>1053_bases
ATGAACAACAACATAACGCTCGAAGAAATTGCGCGCCTGCCCACGTCATATCAGGTCGCGCACATCCGAATCGAACCAAA
TATCATCCTCGCCCCGATGGCCGGGGTCACCGATAGCATCTTTCGCCGCATGATCTTGCGGCTGGGTGGCTGTGGCCTGG
TGAGTACCGAGATGACCAATGCGGCCAGTGTTAGCCCCAAAGCCCTGCGCCGACATCGGTTGCTCGACTATTTGCCTGAA
GAGCGGCCTTTGACGATGCAAATCTCCGGCAACGATCCTGATCTGGTGGCAAATGCGGCGCGGGTCGTCGAACAATTGGG
GGCCGACATTATCGACATCAACTGTGGCTGCCCATCACCCAAAGTGACCGGTGGTGGCCACGGATCAGCGCTGCTGCGCG
ATCTACCGAAGATGGAACGGCTCTTGCGCGCCGTGCGCGAGGCGGTCCAGATTCCGGTCACCCTCAAGTTTCGTGCCGGC
TGGGACGAACAGAGCCTCAACTTTATTGAAGCCGGGAAGCGGGCTGAAGCAGCCGGCGTATCTGCACTGGCCCTGCATCC
ACGGACGCGCGAACAGGGGTACAAAGGTCAGGCCGACTGGTCGCGGGTAGCTGCACTCAAACAGGCCGTCTCCATCCCGG
TGATCGGAAGTGGCGACGTGACCAGCGCTCACGACGCGCTTATCCGTCTGCGCGACAGCGGTGCCGACGGTGTCATGATC
GGACGGGGAGCAATGGCCAACCCCTGGATATTTCGCCAGATCGCCGATCTGAGGCGGGGAGTGACGCCATACGTACCGAC
GCCGGCAGACAAACGCAACCTCTTGATCGAATACATGACCATTTGCGCCGAGGAACTGCCAGAGCGGCTGGCGCTTAACA
AGATCAAACAGTTGATCGGTCAGTTTCATGTTGGCCTGCCCGGCAGCAACCACCTGCGGGTAGCCGTTCACACATCAACC
AGCCTGGCCGAGGCACGTGACGCCATCGAACGATTTTTTGCCCCATTCCTCGAATCAGACCGAGTCGTGCAGGAAATGGT
AGCGGCGGATTAG

Upstream 100 bases:

>100_bases
TGTAAAAAAGACATCATTTGTACCGCAATCGATCATATCATGCCCCCTGGATTGCGAGAGTATGTCAAGCGGGGTCCACA
CTCTCTGCTATAATGATGGT

Downstream 100 bases:

>100_bases
CCGTTTGCCGGATAAGCGGTAGCCGGTGACACGGAAACCTGTTCACCGACTACCTGACCTGAACGACAGTTGAGCAGAGC
CTGCCGACCAACACCCCAAT

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNNNITLEEIARLPTSYQVAHIRIEPNIILAPMAGVTDSIFRRMILRLGGCGLVSTEMTNAASVSPKALRRHRLLDYLPE
ERPLTMQISGNDPDLVANAARVVEQLGADIIDINCGCPSPKVTGGGHGSALLRDLPKMERLLRAVREAVQIPVTLKFRAG
WDEQSLNFIEAGKRAEAAGVSALALHPRTREQGYKGQADWSRVAALKQAVSIPVIGSGDVTSAHDALIRLRDSGADGVMI
GRGAMANPWIFRQIADLRRGVTPYVPTPADKRNLLIEYMTICAEELPERLALNKIKQLIGQFHVGLPGSNHLRVAVHTST
SLAEARDAIERFFAPFLESDRVVQEMVAAD

Sequences:

>Translated_350_residues
MNNNITLEEIARLPTSYQVAHIRIEPNIILAPMAGVTDSIFRRMILRLGGCGLVSTEMTNAASVSPKALRRHRLLDYLPE
ERPLTMQISGNDPDLVANAARVVEQLGADIIDINCGCPSPKVTGGGHGSALLRDLPKMERLLRAVREAVQIPVTLKFRAG
WDEQSLNFIEAGKRAEAAGVSALALHPRTREQGYKGQADWSRVAALKQAVSIPVIGSGDVTSAHDALIRLRDSGADGVMI
GRGAMANPWIFRQIADLRRGVTPYVPTPADKRNLLIEYMTICAEELPERLALNKIKQLIGQFHVGLPGSNHLRVAVHTST
SLAEARDAIERFFAPFLESDRVVQEMVAAD
>Mature_350_residues
MNNNITLEEIARLPTSYQVAHIRIEPNIILAPMAGVTDSIFRRMILRLGGCGLVSTEMTNAASVSPKALRRHRLLDYLPE
ERPLTMQISGNDPDLVANAARVVEQLGADIIDINCGCPSPKVTGGGHGSALLRDLPKMERLLRAVREAVQIPVTLKFRAG
WDEQSLNFIEAGKRAEAAGVSALALHPRTREQGYKGQADWSRVAALKQAVSIPVIGSGDVTSAHDALIRLRDSGADGVMI
GRGAMANPWIFRQIADLRRGVTPYVPTPADKRNLLIEYMTICAEELPERLALNKIKQLIGQFHVGLPGSNHLRVAVHTST
SLAEARDAIERFFAPFLESDRVVQEMVAAD

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=246, Percent_Identity=31.3008130081301, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI239788462, Length=261, Percent_Identity=31.0344827586207, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=33.1858407079646, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=32.15859030837, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI8923374, Length=259, Percent_Identity=30.1158301158301, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789660, Length=237, Percent_Identity=41.3502109704641, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788462, Length=317, Percent_Identity=32.807570977918, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI145693211, Length=302, Percent_Identity=30.794701986755, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=236, Percent_Identity=33.0508474576271, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=227, Percent_Identity=32.15859030837, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=30.6603773584906, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=30.6122448979592, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=335, Percent_Identity=28.6567164179104, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=263, Percent_Identity=28.5171102661597, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=235, Percent_Identity=27.2340425531915, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580595, Length=255, Percent_Identity=31.7647058823529, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19920448, Length=255, Percent_Identity=31.7647058823529, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24585320, Length=262, Percent_Identity=29.7709923664122, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=32.15859030837, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45549423, Length=281, Percent_Identity=29.5373665480427, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38212; Mature: 38212

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNITLEEIARLPTSYQVAHIRIEPNIILAPMAGVTDSIFRRMILRLGGCGLVSTEMTN
CCCCCCHHHHHHCCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCC
AASVSPKALRRHRLLDYLPEERPLTMQISGNDPDLVANAARVVEQLGADIIDINCGCPSP
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
KVTGGGHGSALLRDLPKMERLLRAVREAVQIPVTLKFRAGWDEQSLNFIEAGKRAEAAGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCHHHHCCCCHHHCCC
SALALHPRTREQGYKGQADWSRVAALKQAVSIPVIGSGDVTSAHDALIRLRDSGADGVMI
EEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHEEEECCCCCEEEE
GRGAMANPWIFRQIADLRRGVTPYVPTPADKRNLLIEYMTICAEELPERLALNKIKQLIG
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFHVGLPGSNHLRVAVHTSTSLAEARDAIERFFAPFLESDRVVQEMVAAD
HHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNNNITLEEIARLPTSYQVAHIRIEPNIILAPMAGVTDSIFRRMILRLGGCGLVSTEMTN
CCCCCCHHHHHHCCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCC
AASVSPKALRRHRLLDYLPEERPLTMQISGNDPDLVANAARVVEQLGADIIDINCGCPSP
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
KVTGGGHGSALLRDLPKMERLLRAVREAVQIPVTLKFRAGWDEQSLNFIEAGKRAEAAGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCHHHHCCCCHHHCCC
SALALHPRTREQGYKGQADWSRVAALKQAVSIPVIGSGDVTSAHDALIRLRDSGADGVMI
EEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHEEEECCCCCEEEE
GRGAMANPWIFRQIADLRRGVTPYVPTPADKRNLLIEYMTICAEELPERLALNKIKQLIG
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFHVGLPGSNHLRVAVHTSTSLAEARDAIERFFAPFLESDRVVQEMVAAD
HHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]