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The map label for this gene is leuA [H]

Identifier: 222523472

GI number: 222523472

Start: 205198

End: 206904

Strand: Direct

Name: leuA [H]

Synonym: Chy400_0178

Alternate gene names: 222523472

Gene position: 205198-206904 (Clockwise)

Preceding gene: 222523471

Following gene: 222523473

Centisome position: 3.89

GC content: 58.76

Gene sequence:

>1707_bases
ATGAGCAACACGACTGAACCGAGTGTAGAGTACGTCCGTATCTTTGACACAACCCTGCGCGATGGTGAACAGGCTCCCGG
CTGTACGATGACTCTGGAAGAGAAGCTGGAGGTTGCCCGCCAGTTGGCCCGGCTGCGGGTTGACATTATCGAGGCCGGTT
TTCCGGCAGCTTCCCCCGGCGACTGGGCAGCAGTGCACGAAATTGCCCGCGAAATTGGTACTCCCGACGGCCCGATTATC
GCTGCGCTGGCGCGTGCCAATCGCGACGACATCGACAAGGCGTGGAGTGCAATTCAGCCGGCGGCCAAAAAGCGCATTCA
CACGTTTATGTCAACTTCAGACATCCATCTGGAATATCAATTTCGCAAGACACGTGCCGAAGCTCTGGCCCTGATTCACG
AGATGGTCAGCTATGCACGCTCGCTGTGCGAAGATGTCGAATTCTCACCGATGGATGCCGGTCGTACCGATCCGCTCTTC
CTGCGTGAAGCGGTAGCAGTGGCAGTGGCCGCCGGCGCAACGACGCTCAATATTCCTGATACAGTTGGGTACCTCACCCC
TGACGAGTACGGGGCGATGATCCGTGATCTACGCGAGAATGTACCGGGCATCGAACGCTGTATCCTCTCGACCCACTGCC
ACGACGATCTGGGCATGGCGGTCGCCAACTCGCTGGCCGGCGTGCGGGCAGGGGCGCGGCAGGTCGAATGTACCATCAAC
GGCATCGGCGAACGGGCCGGTAACGCTTCGCTCGAAGAAGTGGTGATGGCCCTGCACACTCGTCAGCAGTACTACGGTCT
GCGCACTCGCGTAGAGACGCGCGAAATTGCTCGCACATCGCGCCTGGTGAGCAGCTTCATCGGCGTACCGGTACCGCCCA
ACAAGGCCATTGTCGGTGCCAATGCCTTCGCCCACGAGTCGGGCATTCACCAGGATGGCGTCTTGAAGTACCGGCAAACG
TATGAAATTATGAGTGCCGAGACGGTTGGTCTCGACAGCAATACCCTGGTGCTCGGCAAGCACTCTGGACGCCACGCTTT
CCGCAAGTATCTGGAAGAGAAGGGCTACAAGCTCAATGAGGAAGAATTCCAGCACGTCTTTGCCCGCTTCAAAGACTTGT
GTGACCGCAAGAAGAAGGTTGATGACCGCGATATCGAGGCGCTGATCGCCGGTGAGATGCAGCATACGCCTGAACTATAC
AAACTCGATCACGTCCAGTATGCGTCGGGTATCGGCATGATCCCCACGGCGACTGTACGGCTGATCGGCCCTGATGGGCA
GACCAGGATTGACAGCGCCCAGGGTACCGGCCCGGTTGATGCGGTCTACCAGGCAATCAACCGCATTATTCAGCGTCCGA
ACGAGTTGATCGAGTTCTCGATCAATGCGATCACCGAAGGGCTTGATGCAGTCGCTGAAGTGACTGTGCGTATTCGCGAA
CAGGGTGCACCGGCCCGGTTGAACGAGAGCAGCAGTACCGCAACCAGCCTGACCGGTCGCCGACCGGCGCAGCAAATCTT
TAGTGGGTACGGTGTACATACCGATACGATTGTGGCCGCTGCGCAAGCCTACATGGCGGCCCTCAACAAGATGCTGGCTG
CCCGTCAGGAGCGAATCAAGGCTGAGGCAGTGGCGTATGCCGCCGGCTACAGCGGAGAAAGCGGGACACTGCCGGTTGAC
ATCTTCGGCGGTAGTACGCTTGGGTAG

Upstream 100 bases:

>100_bases
AAGTCAAGCCGGGTGAAGGTGGCGCCTGAGAGTAGATTGCATAGATCGTGGCCGGTAGTTGCTCCGGCCACGACACACCA
CCACATCACAGGAGGTACCC

Downstream 100 bases:

>100_bases
ATGATCTTGTGCGATCCTGACGGGAAGCATATACTTCCCGCCAGGATCGGTTAACCACGACACCGGGATGCCACAAGCGT
AACCTGGCATCTCATCTGCA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSNTTEPSVEYVRIFDTTLRDGEQAPGCTMTLEEKLEVARQLARLRVDIIEAGFPAASPGDWAAVHEIAREIGTPDGPII
AALARANRDDIDKAWSAIQPAAKKRIHTFMSTSDIHLEYQFRKTRAEALALIHEMVSYARSLCEDVEFSPMDAGRTDPLF
LREAVAVAVAAGATTLNIPDTVGYLTPDEYGAMIRDLRENVPGIERCILSTHCHDDLGMAVANSLAGVRAGARQVECTIN
GIGERAGNASLEEVVMALHTRQQYYGLRTRVETREIARTSRLVSSFIGVPVPPNKAIVGANAFAHESGIHQDGVLKYRQT
YEIMSAETVGLDSNTLVLGKHSGRHAFRKYLEEKGYKLNEEEFQHVFARFKDLCDRKKKVDDRDIEALIAGEMQHTPELY
KLDHVQYASGIGMIPTATVRLIGPDGQTRIDSAQGTGPVDAVYQAINRIIQRPNELIEFSINAITEGLDAVAEVTVRIRE
QGAPARLNESSSTATSLTGRRPAQQIFSGYGVHTDTIVAAAQAYMAALNKMLAARQERIKAEAVAYAAGYSGESGTLPVD
IFGGSTLG

Sequences:

>Translated_568_residues
MSNTTEPSVEYVRIFDTTLRDGEQAPGCTMTLEEKLEVARQLARLRVDIIEAGFPAASPGDWAAVHEIAREIGTPDGPII
AALARANRDDIDKAWSAIQPAAKKRIHTFMSTSDIHLEYQFRKTRAEALALIHEMVSYARSLCEDVEFSPMDAGRTDPLF
LREAVAVAVAAGATTLNIPDTVGYLTPDEYGAMIRDLRENVPGIERCILSTHCHDDLGMAVANSLAGVRAGARQVECTIN
GIGERAGNASLEEVVMALHTRQQYYGLRTRVETREIARTSRLVSSFIGVPVPPNKAIVGANAFAHESGIHQDGVLKYRQT
YEIMSAETVGLDSNTLVLGKHSGRHAFRKYLEEKGYKLNEEEFQHVFARFKDLCDRKKKVDDRDIEALIAGEMQHTPELY
KLDHVQYASGIGMIPTATVRLIGPDGQTRIDSAQGTGPVDAVYQAINRIIQRPNELIEFSINAITEGLDAVAEVTVRIRE
QGAPARLNESSSTATSLTGRRPAQQIFSGYGVHTDTIVAAAQAYMAALNKMLAARQERIKAEAVAYAAGYSGESGTLPVD
IFGGSTLG
>Mature_567_residues
SNTTEPSVEYVRIFDTTLRDGEQAPGCTMTLEEKLEVARQLARLRVDIIEAGFPAASPGDWAAVHEIAREIGTPDGPIIA
ALARANRDDIDKAWSAIQPAAKKRIHTFMSTSDIHLEYQFRKTRAEALALIHEMVSYARSLCEDVEFSPMDAGRTDPLFL
REAVAVAVAAGATTLNIPDTVGYLTPDEYGAMIRDLRENVPGIERCILSTHCHDDLGMAVANSLAGVRAGARQVECTING
IGERAGNASLEEVVMALHTRQQYYGLRTRVETREIARTSRLVSSFIGVPVPPNKAIVGANAFAHESGIHQDGVLKYRQTY
EIMSAETVGLDSNTLVLGKHSGRHAFRKYLEEKGYKLNEEEFQHVFARFKDLCDRKKKVDDRDIEALIAGEMQHTPELYK
LDHVQYASGIGMIPTATVRLIGPDGQTRIDSAQGTGPVDAVYQAINRIIQRPNELIEFSINAITEGLDAVAEVTVRIREQ
GAPARLNESSSTATSLTGRRPAQQIFSGYGVHTDTIVAAAQAYMAALNKMLAARQERIKAEAVAYAAGYSGESGTLPVDI
FGGSTLG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=526, Percent_Identity=45.4372623574144, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=27.2486772486773, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=26.984126984127, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=320, Percent_Identity=29.6875, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=320, Percent_Identity=29.6875, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24582381, Length=295, Percent_Identity=25.7627118644068, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61955; Mature: 61824

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTTEPSVEYVRIFDTTLRDGEQAPGCTMTLEEKLEVARQLARLRVDIIEAGFPAASPG
CCCCCCCCHHEEEEEHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DWAAVHEIAREIGTPDGPIIAALARANRDDIDKAWSAIQPAAKKRIHTFMSTSDIHLEYQ
CHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEH
FRKTRAEALALIHEMVSYARSLCEDVEFSPMDAGRTDPLFLREAVAVAVAAGATTLNIPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCC
TVGYLTPDEYGAMIRDLRENVPGIERCILSTHCHDDLGMAVANSLAGVRAGARQVECTIN
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEE
GIGERAGNASLEEVVMALHTRQQYYGLRTRVETREIARTSRLVSSFIGVPVPPNKAIVGA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
NAFAHESGIHQDGVLKYRQTYEIMSAETVGLDSNTLVLGKHSGRHAFRKYLEEKGYKLNE
CHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCH
EEFQHVFARFKDLCDRKKKVDDRDIEALIAGEMQHTPELYKLDHVQYASGIGMIPTATVR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHCHHHHHCCCCCCCCCEEE
LIGPDGQTRIDSAQGTGPVDAVYQAINRIIQRPNELIEFSINAITEGLDAVAEVTVRIRE
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGAPARLNESSSTATSLTGRRPAQQIFSGYGVHTDTIVAAAQAYMAALNKMLAARQERIK
CCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAVAYAAGYSGESGTLPVDIFGGSTLG
HHHHHHCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SNTTEPSVEYVRIFDTTLRDGEQAPGCTMTLEEKLEVARQLARLRVDIIEAGFPAASPG
CCCCCCCHHEEEEEHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DWAAVHEIAREIGTPDGPIIAALARANRDDIDKAWSAIQPAAKKRIHTFMSTSDIHLEYQ
CHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEH
FRKTRAEALALIHEMVSYARSLCEDVEFSPMDAGRTDPLFLREAVAVAVAAGATTLNIPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCC
TVGYLTPDEYGAMIRDLRENVPGIERCILSTHCHDDLGMAVANSLAGVRAGARQVECTIN
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEE
GIGERAGNASLEEVVMALHTRQQYYGLRTRVETREIARTSRLVSSFIGVPVPPNKAIVGA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
NAFAHESGIHQDGVLKYRQTYEIMSAETVGLDSNTLVLGKHSGRHAFRKYLEEKGYKLNE
CHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCH
EEFQHVFARFKDLCDRKKKVDDRDIEALIAGEMQHTPELYKLDHVQYASGIGMIPTATVR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHCHHHHHCCCCCCCCCEEE
LIGPDGQTRIDSAQGTGPVDAVYQAINRIIQRPNELIEFSINAITEGLDAVAEVTVRIRE
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGAPARLNESSSTATSLTGRRPAQQIFSGYGVHTDTIVAAAQAYMAALNKMLAARQERIK
CCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAVAYAAGYSGESGTLPVDIFGGSTLG
HHHHHHCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]