Click here to switch to the map view.

The map label for this gene is ilvB1 [H]

Identifier: 222523470

GI number: 222523470

Start: 201685

End: 203427

Strand: Direct

Name: ilvB1 [H]

Synonym: Chy400_0175

Alternate gene names: 222523470

Gene position: 201685-203427 (Clockwise)

Preceding gene: 222523469

Following gene: 222523471

Centisome position: 3.83

GC content: 58.12

Gene sequence:

>1743_bases
ATGACCAAACCTCTTACCGGCGCCCAGATTATGTGTGAAGCCCTCATCCGGGAGGGAGTCGAAGTGATGTTCGGCATTCC
CGGCGGTGCCATCATGCCCTTCTACTACGCGATGTGGGATTATCGCGACCGGCTGCGCCACGTGTTGTGTCGCCACGAGC
AGGGAGCCGGTCACGCCGCCGAGGGGTATGCCCGTGCCACCGGCAAGATCGGGGTCTGTATCGGCACCAGTGGCCCCGGC
GCGACCAATCTGGTTACGCCAATTGCCGATGCGATGATGGACAGTACGCCCTTGCTGGCAATTACCGGACAGGTCGGCAG
TCACGTTCTCGGTAAGGATGCCTTCCAGGAGACGGACATTACCGGAATTACGATGCCGATTACCAAGCACAATTATCTGG
TGAAGAATGTCGATGAGCTGGCCTATGTCTTCAAAGAGGCAATTTACATCGCAACAACCGGACGACCCGGCCCGGTGCTG
ATCGACATTACCAAAGATGCAATGCAGAAGACGACGGTACCGAACTGGGACATCAAGCTCAATCTGCCCGGTTACAAGCC
AACCTATCATGGAAACCGTCGTCAGATTCGGGAAGCGATTAAGCTGCTGATCAGCGCGAAGAAGCCGTTGATCATCGCCG
GTCACGGCATCATCATGGCGGGTGCGCACCGCGAGCTGCAAGAGTTTGCCGAACGGTTGCGGATTCCGGTGATTACCACC
CTGCACGGCATTGGCGCTATCCCCGAAAATCATCCGTTGAGCATTGGTATGCCGGGTATGCACGGCTGGGTGCATGTCAA
TCGCGCTATTCAGGAGTGTGATGTTCTCTTTAACATCGGCGGTCGTTTCGACGACCGGGTGACCGGTAAGGCCAGTACCT
TTGCTCCCAACGCCCGCATCATTCACGTTGACATTGATCCCTCTGAGATTGGTAAGAACGTGCGAGTTGACGTGCCGATT
GTCGGCGACGCCCGGCTGGTGTTGCAGGCATTCCTGGAGGATTTGCCCCCACCCAGCGAGCTGGCCGATCTGCACACGCA
TGCTTCGGACTGGATGGAGCACATCCGCGAGATGCAGGATAAGCACCAGGGGAAGCAGCAGTACAAGAACCGGGCCGCGA
TGGTAAATATGGCCTTGCCGCCGCACGATGTCTACGAGGCGCTGAGCCGCACTCTGAACGCACGTGGCAACTATCGGGTG
GTCACCGATGTCGGTCAGCACCAAATGTGGGCAGCCCAATTGATCGACTGGAACCACGGCCCACGCACGCATATTACATC
GGGTGGTGCCGGAACGATGGGTTTTGCAGTGCCGGCAGCCATGGGCGTGGCAATCGCCTGCCCCAACGATACGGTCTGGG
CTATTGTCGGTGACGGTGGCTTCCAGATGACCAATCAAGAGATGGCGACCATCGTGCAGGAAGGGTTGAAGAATGTCAAG
ATCGCCGTCATCAACAACGGCTATCTCGGCATGGTGCGGCAGTGGCAGGAGCTGTTCGAGCACAAGCGGTACAGCGGGAC
ACCTCTCTCTGGCCCCGACTTTGCCAAACTGGCAGAGGCTTACGGCTGGAAGGGTCTGACCGTCGAACGCAGCGAGGATG
TGCAGGATGCCATTGATGAAGCCCACGCGACCGATGGCCCAGTCCTGATCGATTTCCGGGTCGAACGGGAGGTCAACGTC
TTCCCGATGGTGCCCCAGAACAAGTCAATCGGCGAGATGATCCTGTCTGAGTCGCAGGCGTAG

Upstream 100 bases:

>100_bases
CGTTTGGAACGGCATAGCGCAGGGTTGCGCTAATGACCGCTACCTATGGCTGTGTCTCAAAAGCTCGGTTACAGATTCAT
TGTGGACAGGAGTAAGTTCG

Downstream 100 bases:

>100_bases
GGCGCAGACAACCTTGTGAGATGAAGGAGTAGCAGACAATGAAGAAACATACCATCGTCGCCCTCGTGCAAGATCGACCC
GGTGTGCTGAGTCGTGTGAC

Product: acetolactate synthase large subunit, biosynthetic type

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MTKPLTGAQIMCEALIREGVEVMFGIPGGAIMPFYYAMWDYRDRLRHVLCRHEQGAGHAAEGYARATGKIGVCIGTSGPG
ATNLVTPIADAMMDSTPLLAITGQVGSHVLGKDAFQETDITGITMPITKHNYLVKNVDELAYVFKEAIYIATTGRPGPVL
IDITKDAMQKTTVPNWDIKLNLPGYKPTYHGNRRQIREAIKLLISAKKPLIIAGHGIIMAGAHRELQEFAERLRIPVITT
LHGIGAIPENHPLSIGMPGMHGWVHVNRAIQECDVLFNIGGRFDDRVTGKASTFAPNARIIHVDIDPSEIGKNVRVDVPI
VGDARLVLQAFLEDLPPPSELADLHTHASDWMEHIREMQDKHQGKQQYKNRAAMVNMALPPHDVYEALSRTLNARGNYRV
VTDVGQHQMWAAQLIDWNHGPRTHITSGGAGTMGFAVPAAMGVAIACPNDTVWAIVGDGGFQMTNQEMATIVQEGLKNVK
IAVINNGYLGMVRQWQELFEHKRYSGTPLSGPDFAKLAEAYGWKGLTVERSEDVQDAIDEAHATDGPVLIDFRVEREVNV
FPMVPQNKSIGEMILSESQA

Sequences:

>Translated_580_residues
MTKPLTGAQIMCEALIREGVEVMFGIPGGAIMPFYYAMWDYRDRLRHVLCRHEQGAGHAAEGYARATGKIGVCIGTSGPG
ATNLVTPIADAMMDSTPLLAITGQVGSHVLGKDAFQETDITGITMPITKHNYLVKNVDELAYVFKEAIYIATTGRPGPVL
IDITKDAMQKTTVPNWDIKLNLPGYKPTYHGNRRQIREAIKLLISAKKPLIIAGHGIIMAGAHRELQEFAERLRIPVITT
LHGIGAIPENHPLSIGMPGMHGWVHVNRAIQECDVLFNIGGRFDDRVTGKASTFAPNARIIHVDIDPSEIGKNVRVDVPI
VGDARLVLQAFLEDLPPPSELADLHTHASDWMEHIREMQDKHQGKQQYKNRAAMVNMALPPHDVYEALSRTLNARGNYRV
VTDVGQHQMWAAQLIDWNHGPRTHITSGGAGTMGFAVPAAMGVAIACPNDTVWAIVGDGGFQMTNQEMATIVQEGLKNVK
IAVINNGYLGMVRQWQELFEHKRYSGTPLSGPDFAKLAEAYGWKGLTVERSEDVQDAIDEAHATDGPVLIDFRVEREVNV
FPMVPQNKSIGEMILSESQA
>Mature_579_residues
TKPLTGAQIMCEALIREGVEVMFGIPGGAIMPFYYAMWDYRDRLRHVLCRHEQGAGHAAEGYARATGKIGVCIGTSGPGA
TNLVTPIADAMMDSTPLLAITGQVGSHVLGKDAFQETDITGITMPITKHNYLVKNVDELAYVFKEAIYIATTGRPGPVLI
DITKDAMQKTTVPNWDIKLNLPGYKPTYHGNRRQIREAIKLLISAKKPLIIAGHGIIMAGAHRELQEFAERLRIPVITTL
HGIGAIPENHPLSIGMPGMHGWVHVNRAIQECDVLFNIGGRFDDRVTGKASTFAPNARIIHVDIDPSEIGKNVRVDVPIV
GDARLVLQAFLEDLPPPSELADLHTHASDWMEHIREMQDKHQGKQQYKNRAAMVNMALPPHDVYEALSRTLNARGNYRVV
TDVGQHQMWAAQLIDWNHGPRTHITSGGAGTMGFAVPAAMGVAIACPNDTVWAIVGDGGFQMTNQEMATIVQEGLKNVKI
AVINNGYLGMVRQWQELFEHKRYSGTPLSGPDFAKLAEAYGWKGLTVERSEDVQDAIDEAHATDGPVLIDFRVEREVNVF
PMVPQNKSIGEMILSESQA

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=572, Percent_Identity=26.2237762237762, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=25.963149078727, Blast_Score=154, Evalue=3e-37,
Organism=Escherichia coli, GI87081685, Length=581, Percent_Identity=41.8244406196213, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1790104, Length=573, Percent_Identity=41.0122164048866, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=36.4768683274021, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1787096, Length=574, Percent_Identity=29.2682926829268, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788716, Length=567, Percent_Identity=25.3968253968254, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=566, Percent_Identity=27.0318021201413, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=566, Percent_Identity=27.0318021201413, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=582, Percent_Identity=23.5395189003436, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=591, Percent_Identity=43.4856175972927, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=24.6464646464646, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=26.241134751773, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63680; Mature: 63549

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPLTGAQIMCEALIREGVEVMFGIPGGAIMPFYYAMWDYRDRLRHVLCRHEQGAGHAA
CCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGYARATGKIGVCIGTSGPGATNLVTPIADAMMDSTPLLAITGQVGSHVLGKDAFQETDI
CCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHCCCC
TGITMPITKHNYLVKNVDELAYVFKEAIYIATTGRPGPVLIDITKDAMQKTTVPNWDIKL
CEEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCEEEEECHHHHHHCCCCCCEEEE
NLPGYKPTYHGNRRQIREAIKLLISAKKPLIIAGHGIIMAGAHRELQEFAERLRIPVITT
ECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHCCCCCEEH
LHGIGAIPENHPLSIGMPGMHGWVHVNRAIQECDVLFNIGGRFDDRVTGKASTFAPNARI
HHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEE
IHVDIDPSEIGKNVRVDVPIVGDARLVLQAFLEDLPPPSELADLHTHASDWMEHIREMQD
EEEECCHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KHQGKQQYKNRAAMVNMALPPHDVYEALSRTLNARGNYRVVTDVGQHQMWAAQLIDWNHG
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCC
PRTHITSGGAGTMGFAVPAAMGVAIACPNDTVWAIVGDGGFQMTNQEMATIVQEGLKNVK
CCCEEECCCCCCHHHHHHHHCCEEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHCCEE
IAVINNGYLGMVRQWQELFEHKRYSGTPLSGPDFAKLAEAYGWKGLTVERSEDVQDAIDE
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHH
AHATDGPVLIDFRVEREVNVFPMVPQNKSIGEMILSESQA
HCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
TKPLTGAQIMCEALIREGVEVMFGIPGGAIMPFYYAMWDYRDRLRHVLCRHEQGAGHAA
CCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGYARATGKIGVCIGTSGPGATNLVTPIADAMMDSTPLLAITGQVGSHVLGKDAFQETDI
CCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHCCCC
TGITMPITKHNYLVKNVDELAYVFKEAIYIATTGRPGPVLIDITKDAMQKTTVPNWDIKL
CEEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCEEEEECHHHHHHCCCCCCEEEE
NLPGYKPTYHGNRRQIREAIKLLISAKKPLIIAGHGIIMAGAHRELQEFAERLRIPVITT
ECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHCCCCCEEH
LHGIGAIPENHPLSIGMPGMHGWVHVNRAIQECDVLFNIGGRFDDRVTGKASTFAPNARI
HHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEE
IHVDIDPSEIGKNVRVDVPIVGDARLVLQAFLEDLPPPSELADLHTHASDWMEHIREMQD
EEEECCHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KHQGKQQYKNRAAMVNMALPPHDVYEALSRTLNARGNYRVVTDVGQHQMWAAQLIDWNHG
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCC
PRTHITSGGAGTMGFAVPAAMGVAIACPNDTVWAIVGDGGFQMTNQEMATIVQEGLKNVK
CCCEEECCCCCCHHHHHHHHCCEEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHCCEE
IAVINNGYLGMVRQWQELFEHKRYSGTPLSGPDFAKLAEAYGWKGLTVERSEDVQDAIDE
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHH
AHATDGPVLIDFRVEREVNVFPMVPQNKSIGEMILSESQA
HCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]