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The map label for this gene is btuF [H]

Identifier: 222523352

GI number: 222523352

Start: 56273

End: 57055

Strand: Direct

Name: btuF [H]

Synonym: Chy400_0053

Alternate gene names: 222523352

Gene position: 56273-57055 (Clockwise)

Preceding gene: 222523351

Following gene: 222523353

Centisome position: 1.07

GC content: 61.05

Gene sequence:

>783_bases
ATGCTGGTCTACGACGCGCTTGGCCGGGAGATCGAGCTGGCAGGAACACCACAACGAATCGTGTCGCTTGTGCCGAGTGT
AACTGAGTGGTTGTTCAGCGTTGGTGCCGGCGAGCGCGTGATTGGCATCACCGACTACTGTATCGAACCGGCGGCAGCAC
TCCAGCATCTACCCCGGCTCCGCGGCACCAAAAATCCTGATCGAGCGGCAATCATTGCGCTTCAGCCCGATCTGATCATC
GCCGAACAGGAAGAAAACCGGGAACGCGATGTACAGGCACTGACAGCAGCCGGCCTGGCAGTGTATGTCACCGCAATTCG
GAGTGTCGCCGACGCAATGACGCAACTGGCGGCGCTGGCCGAGGTGCTGGACGTAACAGATGGTGCTGCACCGATCACTC
AGGCCATCGAACAGACAGTGACTGCACCGCCACCTCCCTCCCGACGAGTCCTGGCCCTGATCTGGCGCGATCCGTGGATG
GCTATCGGGCGCGACACCTACGCCGGTGACTTGCTTCGCCTGAGCGGTGGGTTCAACGTCGCGGCCACGTTACCCGGACG
CTATCCACGGGCAGCGCTGGCCGACTTTCTGGCCCTCGATCCCGACATTATCTTGTTGCCCAATGAACCATACCCGTTCA
GCGAACGCGACCTGCCGGCATTCAACGCCGTAGCATATCGTGGCCGGATCGTGCTGTGTGATGGGATGGCGTTGACCTGG
CCCGGCCCACGCACAGCCACTGCCATCACAACATTTCGGCGCATTCTGGAGCAGACTACATGA

Upstream 100 bases:

>100_bases
GCCGCCTCTCAACTGTGACGCTGTTGAATGTGACTGCCGGCGGCGGCAGTACCGTTCCGGTCTTCATCATTGACGAGTAA
ACGCAACGCAGGAGTCGGTG

Downstream 100 bases:

>100_bases
ACCCATTCATCCGGTTGCGCGAAGCGGTGATCGATACCGTATTGAGCATAAACGAGCAGCAGGTGCTCAATGCCGGCACC
GGAACCGTACCTGCACCTAC

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLVYDALGREIELAGTPQRIVSLVPSVTEWLFSVGAGERVIGITDYCIEPAAALQHLPRLRGTKNPDRAAIIALQPDLII
AEQEENRERDVQALTAAGLAVYVTAIRSVADAMTQLAALAEVLDVTDGAAPITQAIEQTVTAPPPPSRRVLALIWRDPWM
AIGRDTYAGDLLRLSGGFNVAATLPGRYPRAALADFLALDPDIILLPNEPYPFSERDLPAFNAVAYRGRIVLCDGMALTW
PGPRTATAITTFRRILEQTT

Sequences:

>Translated_260_residues
MLVYDALGREIELAGTPQRIVSLVPSVTEWLFSVGAGERVIGITDYCIEPAAALQHLPRLRGTKNPDRAAIIALQPDLII
AEQEENRERDVQALTAAGLAVYVTAIRSVADAMTQLAALAEVLDVTDGAAPITQAIEQTVTAPPPPSRRVLALIWRDPWM
AIGRDTYAGDLLRLSGGFNVAATLPGRYPRAALADFLALDPDIILLPNEPYPFSERDLPAFNAVAYRGRIVLCDGMALTW
PGPRTATAITTFRRILEQTT
>Mature_260_residues
MLVYDALGREIELAGTPQRIVSLVPSVTEWLFSVGAGERVIGITDYCIEPAAALQHLPRLRGTKNPDRAAIIALQPDLII
AEQEENRERDVQALTAAGLAVYVTAIRSVADAMTQLAALAEVLDVTDGAAPITQAIEQTVTAPPPPSRRVLALIWRDPWM
AIGRDTYAGDLLRLSGGFNVAATLPGRYPRAALADFLALDPDIILLPNEPYPFSERDLPAFNAVAYRGRIVLCDGMALTW
PGPRTATAITTFRRILEQTT

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=197, Percent_Identity=27.4111675126904, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 28180; Mature: 28180

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVYDALGREIELAGTPQRIVSLVPSVTEWLFSVGAGERVIGITDYCIEPAAALQHLPRL
CEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHH
RGTKNPDRAAIIALQPDLIIAEQEENRERDVQALTAAGLAVYVTAIRSVADAMTQLAALA
CCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLDVTDGAAPITQAIEQTVTAPPPPSRRVLALIWRDPWMAIGRDTYAGDLLRLSGGFNV
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCEEEECCCCEE
AATLPGRYPRAALADFLALDPDIILLPNEPYPFSERDLPAFNAVAYRGRIVLCDGMALTW
EEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCEEEEECCEEEEC
PGPRTATAITTFRRILEQTT
CCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVYDALGREIELAGTPQRIVSLVPSVTEWLFSVGAGERVIGITDYCIEPAAALQHLPRL
CEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHH
RGTKNPDRAAIIALQPDLIIAEQEENRERDVQALTAAGLAVYVTAIRSVADAMTQLAALA
CCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLDVTDGAAPITQAIEQTVTAPPPPSRRVLALIWRDPWMAIGRDTYAGDLLRLSGGFNV
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCEEEECCCCEE
AATLPGRYPRAALADFLALDPDIILLPNEPYPFSERDLPAFNAVAYRGRIVLCDGMALTW
EEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCEEEEECCEEEEC
PGPRTATAITTFRRILEQTT
CCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA