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The map label for this gene is psrB [H]

Identifier: 222523325

GI number: 222523325

Start: 26671

End: 27627

Strand: Direct

Name: psrB [H]

Synonym: Chy400_0026

Alternate gene names: 222523325

Gene position: 26671-27627 (Clockwise)

Preceding gene: 222523324

Following gene: 222523326

Centisome position: 0.51

GC content: 61.34

Gene sequence:

>957_bases
ATGACCCGGCAACCAACTATCCCCTTAACCCCGGTAAGCCGCAGAGGTTTACTCGGTGCACTGGTAGCCGGCGCCGGTGC
CCTCGCTGCGGCTTCAGTACAGGCAGGTCTCCCTGCCAGCACCGGAAAATCAGCCGACACTATCGATGTGCTTTCGCCTG
AAGAAGCCCTGGCCGCCTACGAAGCCGAGATTGCTCGCCTCCGTGCAACCAAAGTGAGCGGTACACCCCTCGATCATCTG
GAGCGGATGCGGGCCGAACTGCTGCGGGCACTCGCCAAACCGGTCGCCGAACGCCGTTGGGTGATGGTGATTGATCTCCG
CGCCTGCGTCGGTTGTCACGGTTGCACCATCGCCTGCGTGGCCGAGAACAAACTGCCACCCGGCGTGGTCTACCGACCGG
TGATCGACGAAGAGATCGGTCGCTACCCCAATGTCACGCGCCGCTTCATTCCCCGACCCTGTATGCAATGCGACAATCCG
CCCTGCACCGGGGTTTGCCCGGTCTCGGCCACCTTCACCAACGAGCATGGCGTCGTCGAAGTAGACTACGAGCAGTGCAT
TGGCTGCCGTGCCTGTATCGCTGCGTGTCCGTATGGTGCGCGCACCTTCGATTTCGGATACACCTACACCGCCGACACCA
CCGACCCTGGCGTCATGTTTGGCGCCGATCAGGCAGCGACCTACGAGACGGTCACCACCTTCGAGTACGGCAAAGCCCAC
GCACGGCGCAACCTCCACGACTCGCCGGTTGGGAACGTGCGTAAATGCCACTTCTGCATGCACCGGGTACTCAACGGTCA
ACTCCCGGCCTGTGTCACGACCTGCATCGGGCGCGCCACCTTCTTCGGTGATGCCAATGATCCCGATAGCCTGGTTAGCG
AACTGATTGCCAAACCCAATGTCATGCGGTTGAAAGAAGACCTCGGCACCGAGCCGCGTGTCTACTATTTGCAGTGA

Upstream 100 bases:

>100_bases
CTGACTTTTCCTGCTACCGGTGGCACCGGCACAGATGTAAAGTGGGATTAGCGGGAATGCCGTAGATAAGCAATCCGCAT
TAGTACAAGGAGCCTGGACT

Downstream 100 bases:

>100_bases
GGAGCAGACCTATGCTTAAGCGACTCCTCTATCTCGCCGGCACCCTTGGCCTGCTCGTCGGCCTGTGGGGATTGTACGAC
CGCCTTGTCTACGGTCATAT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTRQPTIPLTPVSRRGLLGALVAGAGALAAASVQAGLPASTGKSADTIDVLSPEEALAAYEAEIARLRATKVSGTPLDHL
ERMRAELLRALAKPVAERRWVMVIDLRACVGCHGCTIACVAENKLPPGVVYRPVIDEEIGRYPNVTRRFIPRPCMQCDNP
PCTGVCPVSATFTNEHGVVEVDYEQCIGCRACIAACPYGARTFDFGYTYTADTTDPGVMFGADQAATYETVTTFEYGKAH
ARRNLHDSPVGNVRKCHFCMHRVLNGQLPACVTTCIGRATFFGDANDPDSLVSELIAKPNVMRLKEDLGTEPRVYYLQ

Sequences:

>Translated_318_residues
MTRQPTIPLTPVSRRGLLGALVAGAGALAAASVQAGLPASTGKSADTIDVLSPEEALAAYEAEIARLRATKVSGTPLDHL
ERMRAELLRALAKPVAERRWVMVIDLRACVGCHGCTIACVAENKLPPGVVYRPVIDEEIGRYPNVTRRFIPRPCMQCDNP
PCTGVCPVSATFTNEHGVVEVDYEQCIGCRACIAACPYGARTFDFGYTYTADTTDPGVMFGADQAATYETVTTFEYGKAH
ARRNLHDSPVGNVRKCHFCMHRVLNGQLPACVTTCIGRATFFGDANDPDSLVSELIAKPNVMRLKEDLGTEPRVYYLQ
>Mature_317_residues
TRQPTIPLTPVSRRGLLGALVAGAGALAAASVQAGLPASTGKSADTIDVLSPEEALAAYEAEIARLRATKVSGTPLDHLE
RMRAELLRALAKPVAERRWVMVIDLRACVGCHGCTIACVAENKLPPGVVYRPVIDEEIGRYPNVTRRFIPRPCMQCDNPP
CTGVCPVSATFTNEHGVVEVDYEQCIGCRACIAACPYGARTFDFGYTYTADTTDPGVMFGADQAATYETVTTFEYGKAHA
RRNLHDSPVGNVRKCHFCMHRVLNGQLPACVTTCIGRATFFGDANDPDSLVSELIAKPNVMRLKEDLGTEPRVYYLQ

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=220, Percent_Identity=35.4545454545455, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI226510944, Length=213, Percent_Identity=29.1079812206573, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787872, Length=193, Percent_Identity=29.5336787564767, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787122, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789370, Length=207, Percent_Identity=25.6038647342995, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082179, Length=110, Percent_Identity=33.6363636363636, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082180, Length=95, Percent_Identity=37.8947368421053, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=102, Percent_Identity=36.2745098039216, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789067, Length=200, Percent_Identity=27, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 34424; Mature: 34293

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQPTIPLTPVSRRGLLGALVAGAGALAAASVQAGLPASTGKSADTIDVLSPEEALAAY
CCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHH
EAEIARLRATKVSGTPLDHLERMRAELLRALAKPVAERRWVMVIDLRACVGCHGCTIACV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCEEEEE
AENKLPPGVVYRPVIDEEIGRYPNVTRRFIPRPCMQCDNPPCTGVCPVSATFTNEHGVVE
ECCCCCCCCEECCCHHHHHCCCCCHHHHHCCCHHHCCCCCCCCEECCCEEEECCCCCEEE
VDYEQCIGCRACIAACPYGARTFDFGYTYTADTTDPGVMFGADQAATYETVTTFEYGKAH
ECHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCEEECCCCCCCEEEEEEEHHHHHH
ARRNLHDSPVGNVRKCHFCMHRVLNGQLPACVTTCIGRATFFGDANDPDSLVSELIAKPN
HHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCC
VMRLKEDLGTEPRVYYLQ
HHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
TRQPTIPLTPVSRRGLLGALVAGAGALAAASVQAGLPASTGKSADTIDVLSPEEALAAY
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHH
EAEIARLRATKVSGTPLDHLERMRAELLRALAKPVAERRWVMVIDLRACVGCHGCTIACV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCEEEEE
AENKLPPGVVYRPVIDEEIGRYPNVTRRFIPRPCMQCDNPPCTGVCPVSATFTNEHGVVE
ECCCCCCCCEECCCHHHHHCCCCCHHHHHCCCHHHCCCCCCCCEECCCEEEECCCCCEEE
VDYEQCIGCRACIAACPYGARTFDFGYTYTADTTDPGVMFGADQAATYETVTTFEYGKAH
ECHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCEEECCCCCCCEEEEEEEHHHHHH
ARRNLHDSPVGNVRKCHFCMHRVLNGQLPACVTTCIGRATFFGDANDPDSLVSELIAKPN
HHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCC
VMRLKEDLGTEPRVYYLQ
HHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]