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The map label for this gene is nreB [H]

Identifier: 222523323

GI number: 222523323

Start: 24418

End: 25893

Strand: Direct

Name: nreB [H]

Synonym: Chy400_0024

Alternate gene names: 222523323

Gene position: 24418-25893 (Clockwise)

Preceding gene: 222523322

Following gene: 222523324

Centisome position: 0.46

GC content: 55.76

Gene sequence:

>1476_bases
ATGAACGCAGGGTTCTTGCCACATCAGGTTGTACGATGGGCGACAACGCTATGGCAGATTGTCGGCTCGGTAAATATTCG
CGCCAAAATTCTGGGTATTGTACTGGGACTGGTAGTCTTGATGGGTGTGGCTGCTACCATCGAAGTACGGACACTGCTGG
AGCATACCCTCACTGTGCAAACGCACGAACGGTCAATTGCCATTGCCCGCGACGTTGCCGCCCGCTCTACCGATTTGGTA
CTGATGCGCGATTATTACAGACTCTTCCGCTTGTTGCAAGACACTCAGGAGAATAACCCTGATCTACGCTACGCTTTCAT
CATTGATTCAGAGGGCACGATTGTCGCTCACACTTTTGGCAGCGGCTTTCCGGTCGGTTTGCGCGATGCCAACACGGTAA
CAGCGCAAGAACATCATCGCAGTGTGCTGCTGGCAACGAATGAAGGTGATATTTGGGATACGGCGGTGCCCATGTTCGAC
GGTCGTGCCGGTATCGCCCGAGTCGGCCTCTCGCTGGCCGCCCGTGAACAGACAGTCGCTGCCGTCACCGGGCAGTTGTT
AATCACCACCATTATGGCGGCAGCTATCGGCATCACTGCCGCAGCGTTTCTAACCTGGCTTCTGACCAGGCCCATCTTAC
AACTGGTAGCGCTCACTAAGGCGGTTGCGCTCGGTGATTTCAGTCAGCGTGCCCGACGTTGGGCCAACGATGAAATTGGG
CAGCTCACCGATGCGTTTAACGCGATGAGTGAGGCGCTGGCGCAGGCTGAGCGTGAACGGGCCGAACGTGAACATCTACG
TGCTCAGTATGTCACGCAAATTATTACCGCTCAGGAAGAGGAACGCAAGCGGATCGCCCGTGAACTGCACGATAGCACCA
GTCAGGCTCTGACCTCATTGCTGGTCGGCTTACGCTCACTCGCCGACCGCCACCGCTCGCCGGAGATTGCGCAACAGGTA
GATGATCTGCGGAGCATCGTCGGGCAAATTCTCAACGATCTCCATGCACTGGCCCGGCAACTACGGCCAAGCGTGCTTGA
CGATCTGGGATTGGCGGCTGCCTTGCAACGGTATGTAGCCGAGTGTCGTGCTCGCAGTGGTTTGATCATTGATCTGGCAC
TACCGGGGTTAGATGATTTCCGCCTCGATCCGGTGGTAGAGACAGCACTATACCGGATTGTACAAGAGGGATTAACGAAC
ATCATCCGACATGCCCACGCGAGCACCGCCAGCGTCGTGATTGAACGTCGTGCTGATCGAGTGCGTGCGATCATTGAGGA
TGACGGATGTGGTTTTGATCCGAATGCCCTGCACGGTGACGGTCATCTGGGATTGAACGGCATCCGTGAACGGGCAGCGC
TTTTGAACGGCCAGCTCATCATCGAGTCGTCTCCCGGTAGTGGCACCACGTTGTACGTGGAAATTCCTTTAACGACCAGG
CATGAGGAGTGCTATGAGCAGCATTCTCTTAGTTGA

Upstream 100 bases:

>100_bases
CTGCCTTGGAGTCGTTAGGCATTGATCGCTTCGTCATGATTGACGATGCAGCCTACGCCAGTGCCCGGCTACTACGCTCA
CAAGTAGGACCGGTATCGCC

Downstream 100 bases:

>100_bases
TGATCACGCTGTGCTGCGTGCCGGTTTACGGCTTCTGCTCGACGGGCAACCCGATCTGCGCGTTATCGGCGAGGCGGAAG
ATGTACGGACAGCCGTGGTG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNAGFLPHQVVRWATTLWQIVGSVNIRAKILGIVLGLVVLMGVAATIEVRTLLEHTLTVQTHERSIAIARDVAARSTDLV
LMRDYYRLFRLLQDTQENNPDLRYAFIIDSEGTIVAHTFGSGFPVGLRDANTVTAQEHHRSVLLATNEGDIWDTAVPMFD
GRAGIARVGLSLAAREQTVAAVTGQLLITTIMAAAIGITAAAFLTWLLTRPILQLVALTKAVALGDFSQRARRWANDEIG
QLTDAFNAMSEALAQAERERAEREHLRAQYVTQIITAQEEERKRIARELHDSTSQALTSLLVGLRSLADRHRSPEIAQQV
DDLRSIVGQILNDLHALARQLRPSVLDDLGLAAALQRYVAECRARSGLIIDLALPGLDDFRLDPVVETALYRIVQEGLTN
IIRHAHASTASVVIERRADRVRAIIEDDGCGFDPNALHGDGHLGLNGIRERAALLNGQLIIESSPGSGTTLYVEIPLTTR
HEECYEQHSLS

Sequences:

>Translated_491_residues
MNAGFLPHQVVRWATTLWQIVGSVNIRAKILGIVLGLVVLMGVAATIEVRTLLEHTLTVQTHERSIAIARDVAARSTDLV
LMRDYYRLFRLLQDTQENNPDLRYAFIIDSEGTIVAHTFGSGFPVGLRDANTVTAQEHHRSVLLATNEGDIWDTAVPMFD
GRAGIARVGLSLAAREQTVAAVTGQLLITTIMAAAIGITAAAFLTWLLTRPILQLVALTKAVALGDFSQRARRWANDEIG
QLTDAFNAMSEALAQAERERAEREHLRAQYVTQIITAQEEERKRIARELHDSTSQALTSLLVGLRSLADRHRSPEIAQQV
DDLRSIVGQILNDLHALARQLRPSVLDDLGLAAALQRYVAECRARSGLIIDLALPGLDDFRLDPVVETALYRIVQEGLTN
IIRHAHASTASVVIERRADRVRAIIEDDGCGFDPNALHGDGHLGLNGIRERAALLNGQLIIESSPGSGTTLYVEIPLTTR
HEECYEQHSLS
>Mature_491_residues
MNAGFLPHQVVRWATTLWQIVGSVNIRAKILGIVLGLVVLMGVAATIEVRTLLEHTLTVQTHERSIAIARDVAARSTDLV
LMRDYYRLFRLLQDTQENNPDLRYAFIIDSEGTIVAHTFGSGFPVGLRDANTVTAQEHHRSVLLATNEGDIWDTAVPMFD
GRAGIARVGLSLAAREQTVAAVTGQLLITTIMAAAIGITAAAFLTWLLTRPILQLVALTKAVALGDFSQRARRWANDEIG
QLTDAFNAMSEALAQAERERAEREHLRAQYVTQIITAQEEERKRIARELHDSTSQALTSLLVGLRSLADRHRSPEIAQQV
DDLRSIVGQILNDLHALARQLRPSVLDDLGLAAALQRYVAECRARSGLIIDLALPGLDDFRLDPVVETALYRIVQEGLTN
IIRHAHASTASVVIERRADRVRAIIEDDGCGFDPNALHGDGHLGLNGIRERAALLNGQLIIESSPGSGTTLYVEIPLTTR
HEECYEQHSLS

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=213, Percent_Identity=30.9859154929577, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87082311, Length=224, Percent_Identity=31.25, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788812, Length=249, Percent_Identity=28.9156626506024, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53975; Mature: 53975

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAGFLPHQVVRWATTLWQIVGSVNIRAKILGIVLGLVVLMGVAATIEVRTLLEHTLTVQ
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
THERSIAIARDVAARSTDLVLMRDYYRLFRLLQDTQENNPDLRYAFIIDSEGTIVAHTFG
ECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECC
SGFPVGLRDANTVTAQEHHRSVLLATNEGDIWDTAVPMFDGRAGIARVGLSLAAREQTVA
CCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AVTGQLLITTIMAAAIGITAAAFLTWLLTRPILQLVALTKAVALGDFSQRARRWANDEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHH
QLTDAFNAMSEALAQAERERAEREHLRAQYVTQIITAQEEERKRIARELHDSTSQALTSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LVGLRSLADRHRSPEIAQQVDDLRSIVGQILNDLHALARQLRPSVLDDLGLAAALQRYVA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
ECRARSGLIIDLALPGLDDFRLDPVVETALYRIVQEGLTNIIRHAHASTASVVIERRADR
HHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VRAIIEDDGCGFDPNALHGDGHLGLNGIRERAALLNGQLIIESSPGSGTTLYVEIPLTTR
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCC
HEECYEQHSLS
HHHHHHHHCCC
>Mature Secondary Structure
MNAGFLPHQVVRWATTLWQIVGSVNIRAKILGIVLGLVVLMGVAATIEVRTLLEHTLTVQ
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
THERSIAIARDVAARSTDLVLMRDYYRLFRLLQDTQENNPDLRYAFIIDSEGTIVAHTFG
ECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECC
SGFPVGLRDANTVTAQEHHRSVLLATNEGDIWDTAVPMFDGRAGIARVGLSLAAREQTVA
CCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AVTGQLLITTIMAAAIGITAAAFLTWLLTRPILQLVALTKAVALGDFSQRARRWANDEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHH
QLTDAFNAMSEALAQAERERAEREHLRAQYVTQIITAQEEERKRIARELHDSTSQALTSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LVGLRSLADRHRSPEIAQQVDDLRSIVGQILNDLHALARQLRPSVLDDLGLAAALQRYVA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
ECRARSGLIIDLALPGLDDFRLDPVVETALYRIVQEGLTNIIRHAHASTASVVIERRADR
HHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VRAIIEDDGCGFDPNALHGDGHLGLNGIRERAALLNGQLIIESSPGSGTTLYVEIPLTTR
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCC
HEECYEQHSLS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA