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The map label for this gene is pbpB2 [H]

Identifier: 222109144

GI number: 222109144

Start: 33816

End: 35573

Strand: Reverse

Name: pbpB2 [H]

Synonym: Avi_9532

Alternate gene names: 222109144

Gene position: 35573-33816 (Counterclockwise)

Preceding gene: 222109145

Following gene: 222109143

Centisome position: 27.27

GC content: 54.78

Gene sequence:

>1758_bases
ATGTCGTTTCTTTCTCGTATCATCACCATTAGAACAAAGGCGCATTTTTCGTCGGTTGCGCGTCGTCGATCAGCATCATT
CGGTCCTGGGAAGCATAAAGGTAATGAAGCGAGACAACGCATCCTTGTTCTGATGGGGGGGTTCTGCGTTCTCTATACGG
TGATTGGCGCACGACTTGTCCAATATGGCACTGCATCGCCCCTGGTCACGAGTTCGATCAGTGCTCCTGGGTTTGGCATT
GCTTCACGCCCGGATATCGTGGACCGGAACGGCGAACTCCTTGCCACGGACCTCAATATGGTGTCACTTTATGCGGAACC
AAGGAAGATCGTTGACATTGACGAAGCCGTGGAGAAGCTATCGACCGTCATCAAGGGGATCGACTGGGCCGATACCTACA
AAAAGCTGCAATCCAAATCAGCGTTCCAGTGGCTCCGCCGCCGACTGACGCCGCGCCAGCAAGCAGATATCCTCGCGCTC
GGCATCCCTGGTATTGGGTTCCGTCCCGAGAAACAGCGTTTCTATCCGGGCGGCGCAACGGCAGCCCATATCCTTGGACA
CGTCAACATCGACAATAAGGGGCTCGCAGGGATGGAGCGCTATATCGATCAGCAAGGACTTGCTGATCTGCGTGCCGCAG
GCCTGACCGACAACGCCCACCTCGATCCTGTGCGGCTGTCCATCGATCTAAGGGTGCAGAACATCGTCCATGACGTGATC
GCCGAGGCAATGATCAACTACAAAGCTGAAGCCGCTGGGGCTGTTGTGCTAGACGCCGAGACGGGGGAAATCCTCGCCAT
GGCATCAGTCCCCGACTACAATCCAAACGAACCGTCTAGAACCCTCCCTGACGGCTCTGTCGATAAAGAGTATGAAAAAG
GCTGGTTCAACCGGATGAGCAACGCGACCTACGAAATGGGATCGACGTTCAAAAGCTTCACGCTTGCGATGGGGTTGGAC
GAGGGGAAAATCGGGCTTAATTCTGTCTTGGATGCTTCGAGGCCAATCAAAATGGGCGGTTTTACCATTCATGATGACCA
TGGTCAGGGACGCCCTCTCACTGTCCCGGAGGTCTTCCAATATTCATCAAATATCGGAACTGCCAAGATTGCGACAATGG
TGAATCTTAAAGACCATCAGGCCTTCCTGACCAAGCTGGGCCTTCTGTCGAAGATGACGACTGAACTGCCCGAAGTTGCC
ACCCCCACTCAGCCACGTGTCTGGAAGCCGCTCAATCAAGTGACGATATCCTTCGGCCATGGCGTTGCAACCACACCGCT
GCAAACCGCCGTAGCAGGGGCGGCCCTTATTAACGGTGGAAAGCTCTTTTCACCGACCTTCCTGGCCAGAAGCCCTGATG
AGGCCGATGTCGTGGTGAAGCAGGTCATTCAGCCGAAGACCAGTGCAGACATGCGATACCTCTTCCACTGGAACGGGATC
AAAGGTTCCGGCAGGAACGCACAGGTCCAGGGGTTCAACGTTGGGGGCAAAACGGGTACCGCCGACAAGGTTATTAACGG
TAAATACGCGGCGCACCTGAACTTCAACGCCTATCTGGCCGGATTCCCAATTGACAAGCCTAGATACGTTGTGTTGACCG
TCATCGACGCACCATTAACCGGGGAAAAGGGAGGTAGGCTAGCTGCGTACACCGCTGCCCCGATGGCGAAGGAAGTCATA
AGCAGATCGGCAGCCCTTCTTGGTGTACAGCCTCGCTTCGGGAACGATGACGATCCGCAGTTACTAACTAACTACTGA

Upstream 100 bases:

>100_bases
GATACTCTTTTGACACCAAACAGCGTAGCTGCCGCTTCGCATCCGATTTAGCGTTCCGCAACAAGTTGCCGGGCCGCGCT
TTTTTGTCGAGTGAGCTACC

Downstream 100 bases:

>100_bases
AGATCCTTTTTCGGAGGGGCCTGTAGCGGGCGGTGTTATCCTCGATACCGCTGGAGCAGGTGGAGCGCCGTGAACAGGCA
TCGCCTTGCTGGTAGGTGCT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSFLSRIITIRTKAHFSSVARRRSASFGPGKHKGNEARQRILVLMGGFCVLYTVIGARLVQYGTASPLVTSSISAPGFGI
ASRPDIVDRNGELLATDLNMVSLYAEPRKIVDIDEAVEKLSTVIKGIDWADTYKKLQSKSAFQWLRRRLTPRQQADILAL
GIPGIGFRPEKQRFYPGGATAAHILGHVNIDNKGLAGMERYIDQQGLADLRAAGLTDNAHLDPVRLSIDLRVQNIVHDVI
AEAMINYKAEAAGAVVLDAETGEILAMASVPDYNPNEPSRTLPDGSVDKEYEKGWFNRMSNATYEMGSTFKSFTLAMGLD
EGKIGLNSVLDASRPIKMGGFTIHDDHGQGRPLTVPEVFQYSSNIGTAKIATMVNLKDHQAFLTKLGLLSKMTTELPEVA
TPTQPRVWKPLNQVTISFGHGVATTPLQTAVAGAALINGGKLFSPTFLARSPDEADVVVKQVIQPKTSADMRYLFHWNGI
KGSGRNAQVQGFNVGGKTGTADKVINGKYAAHLNFNAYLAGFPIDKPRYVVLTVIDAPLTGEKGGRLAAYTAAPMAKEVI
SRSAALLGVQPRFGNDDDPQLLTNY

Sequences:

>Translated_585_residues
MSFLSRIITIRTKAHFSSVARRRSASFGPGKHKGNEARQRILVLMGGFCVLYTVIGARLVQYGTASPLVTSSISAPGFGI
ASRPDIVDRNGELLATDLNMVSLYAEPRKIVDIDEAVEKLSTVIKGIDWADTYKKLQSKSAFQWLRRRLTPRQQADILAL
GIPGIGFRPEKQRFYPGGATAAHILGHVNIDNKGLAGMERYIDQQGLADLRAAGLTDNAHLDPVRLSIDLRVQNIVHDVI
AEAMINYKAEAAGAVVLDAETGEILAMASVPDYNPNEPSRTLPDGSVDKEYEKGWFNRMSNATYEMGSTFKSFTLAMGLD
EGKIGLNSVLDASRPIKMGGFTIHDDHGQGRPLTVPEVFQYSSNIGTAKIATMVNLKDHQAFLTKLGLLSKMTTELPEVA
TPTQPRVWKPLNQVTISFGHGVATTPLQTAVAGAALINGGKLFSPTFLARSPDEADVVVKQVIQPKTSADMRYLFHWNGI
KGSGRNAQVQGFNVGGKTGTADKVINGKYAAHLNFNAYLAGFPIDKPRYVVLTVIDAPLTGEKGGRLAAYTAAPMAKEVI
SRSAALLGVQPRFGNDDDPQLLTNY
>Mature_584_residues
SFLSRIITIRTKAHFSSVARRRSASFGPGKHKGNEARQRILVLMGGFCVLYTVIGARLVQYGTASPLVTSSISAPGFGIA
SRPDIVDRNGELLATDLNMVSLYAEPRKIVDIDEAVEKLSTVIKGIDWADTYKKLQSKSAFQWLRRRLTPRQQADILALG
IPGIGFRPEKQRFYPGGATAAHILGHVNIDNKGLAGMERYIDQQGLADLRAAGLTDNAHLDPVRLSIDLRVQNIVHDVIA
EAMINYKAEAAGAVVLDAETGEILAMASVPDYNPNEPSRTLPDGSVDKEYEKGWFNRMSNATYEMGSTFKSFTLAMGLDE
GKIGLNSVLDASRPIKMGGFTIHDDHGQGRPLTVPEVFQYSSNIGTAKIATMVNLKDHQAFLTKLGLLSKMTTELPEVAT
PTQPRVWKPLNQVTISFGHGVATTPLQTAVAGAALINGGKLFSPTFLARSPDEADVVVKQVIQPKTSADMRYLFHWNGIK
GSGRNAQVQGFNVGGKTGTADKVINGKYAAHLNFNAYLAGFPIDKPRYVVLTVIDAPLTGEKGGRLAAYTAAPMAKEVIS
RSAALLGVQPRFGNDDDPQLLTNY

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=539, Percent_Identity=30.2411873840445, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 63377; Mature: 63246

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLSRIITIRTKAHFSSVARRRSASFGPGKHKGNEARQRILVLMGGFCVLYTVIGARLV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QYGTASPLVTSSISAPGFGIASRPDIVDRNGELLATDLNMVSLYAEPRKIVDIDEAVEKL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEECHHHHHHHH
STVIKGIDWADTYKKLQSKSAFQWLRRRLTPRQQADILALGIPGIGFRPEKQRFYPGGAT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
AAHILGHVNIDNKGLAGMERYIDQQGLADLRAAGLTDNAHLDPVRLSIDLRVQNIVHDVI
HHHEEEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHH
AEAMINYKAEAAGAVVLDAETGEILAMASVPDYNPNEPSRTLPDGSVDKEYEKGWFNRMS
HHHHHCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
NATYEMGSTFKSFTLAMGLDEGKIGLNSVLDASRPIKMGGFTIHDDHGQGRPLTVPEVFQ
CCHHHHCCHHHHEEEEECCCCCCCCHHHHHCCCCCEEECCEEEECCCCCCCCCCHHHHHH
YSSNIGTAKIATMVNLKDHQAFLTKLGLLSKMTTELPEVATPTQPRVWKPLNQVTISFGH
HCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECC
GVATTPLQTAVAGAALINGGKLFSPTFLARSPDEADVVVKQVIQPKTSADMRYLFHWNGI
CCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KGSGRNAQVQGFNVGGKTGTADKVINGKYAAHLNFNAYLAGFPIDKPRYVVLTVIDAPLT
CCCCCCCEEEEEECCCCCCCCCCEECCEEEEEEECCEEEEECCCCCCCEEEEEEEECCCC
GEKGGRLAAYTAAPMAKEVISRSAALLGVQPRFGNDDDPQLLTNY
CCCCCEEEEEEHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFLSRIITIRTKAHFSSVARRRSASFGPGKHKGNEARQRILVLMGGFCVLYTVIGARLV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QYGTASPLVTSSISAPGFGIASRPDIVDRNGELLATDLNMVSLYAEPRKIVDIDEAVEKL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEECHHHHHHHH
STVIKGIDWADTYKKLQSKSAFQWLRRRLTPRQQADILALGIPGIGFRPEKQRFYPGGAT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
AAHILGHVNIDNKGLAGMERYIDQQGLADLRAAGLTDNAHLDPVRLSIDLRVQNIVHDVI
HHHEEEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHH
AEAMINYKAEAAGAVVLDAETGEILAMASVPDYNPNEPSRTLPDGSVDKEYEKGWFNRMS
HHHHHCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
NATYEMGSTFKSFTLAMGLDEGKIGLNSVLDASRPIKMGGFTIHDDHGQGRPLTVPEVFQ
CCHHHHCCHHHHEEEEECCCCCCCCHHHHHCCCCCEEECCEEEECCCCCCCCCCHHHHHH
YSSNIGTAKIATMVNLKDHQAFLTKLGLLSKMTTELPEVATPTQPRVWKPLNQVTISFGH
HCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECC
GVATTPLQTAVAGAALINGGKLFSPTFLARSPDEADVVVKQVIQPKTSADMRYLFHWNGI
CCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KGSGRNAQVQGFNVGGKTGTADKVINGKYAAHLNFNAYLAGFPIDKPRYVVLTVIDAPLT
CCCCCCCEEEEEECCCCCCCCCCEECCEEEEEEECCEEEEECCCCCCCEEEEEEEECCCC
GEKGGRLAAYTAAPMAKEVISRSAALLGVQPRFGNDDDPQLLTNY
CCCCCEEEEEEHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]