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The map label for this gene is ytwF [H]

Identifier: 222094530

GI number: 222094530

Start: 851840

End: 852136

Strand: Direct

Name: ytwF [H]

Synonym: BCQ_0868

Alternate gene names: 222094530

Gene position: 851840-852136 (Clockwise)

Preceding gene: 222094529

Following gene: 222094531

Centisome position: 16.34

GC content: 37.71

Gene sequence:

>297_bases
ATGAAAGAAATGACAGCAAAAGAATTAGAAGAAAAATTGTTACGTAAAGAAGCAGTAAATATCGTAGACGTACGTGAAGT
AGAAGAAGTAGCTGCAGGGAAAATTCCAGAAGCGTGTAATATTCCACTAGGACTATTGGAGTTTCGTATGCATGAGTTGG
ATAAAAAGAAAGAGTATATTATCGTTTGTCGTTCTGGCGGAAGAAGTGCACGAGCTGTTCAATTTTTAGAGAGCTACGGT
TTTCAAGTGATCAATATGGTAGGTGGTATGTTAGCGTGGGAAGGTAAAGTAGAATAG

Upstream 100 bases:

>100_bases
TGAGAAGTAAGCAGGCTGCAAAAGTATTAAAAAAATTAGGATTTCAGCACGTTATCAATGTTTCAGGCGGTATGAATGGT
TTGTGAGGAGGAAATGTAAA

Downstream 100 bases:

>100_bases
GGCGAAACAATTTTTTATAAAAATAAAAATACCCCTATAGGTAATTGGAGGGAAAACAACATGGAACAACAGAAGAAAAC
AACAATCGTTTTATTTAGCG

Product: rhodanese-like domain protein (thiosulfate sulfurtransferase)

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MKEMTAKELEEKLLRKEAVNIVDVREVEEVAAGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLESYG
FQVINMVGGMLAWEGKVE

Sequences:

>Translated_98_residues
MKEMTAKELEEKLLRKEAVNIVDVREVEEVAAGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLESYG
FQVINMVGGMLAWEGKVE
>Mature_98_residues
MKEMTAKELEEKLLRKEAVNIVDVREVEEVAAGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLESYG
FQVINMVGGMLAWEGKVE

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11098; Mature: 11098

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMTAKELEEKLLRKEAVNIVDVREVEEVAAGKIPEACNIPLGLLEFRMHELDKKKEYI
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEE
IVCRSGGRSARAVQFLESYGFQVINMVGGMLAWEGKVE
EEECCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKEMTAKELEEKLLRKEAVNIVDVREVEEVAAGKIPEACNIPLGLLEFRMHELDKKKEYI
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEE
IVCRSGGRSARAVQFLESYGFQVINMVGGMLAWEGKVE
EEECCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]