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The map label for this gene is rbsB [H]

Identifier: 222094423

GI number: 222094423

Start: 736252

End: 737178

Strand: Direct

Name: rbsB [H]

Synonym: BCQ_0737

Alternate gene names: 222094423

Gene position: 736252-737178 (Clockwise)

Preceding gene: 222094422

Following gene: 222094424

Centisome position: 14.12

GC content: 39.27

Gene sequence:

>927_bases
ATGAAGAAATGGTTACTTATATTCGTTGCATGTATTATGGTCATTACTGCTGGTTGTTCAATGGAACCACCAGAATGGGC
AAAGGATTCTAGCGATAAAGGTCGAAATAAAACTATTAAAGTTGGATTCTCTGTTTCAACTTTAAATAATCCCTTCTTTG
TAACTTTGAAAAAAGGTGCAGAAAAGAAAGCGAAAGATAGTGGCATCGAATTGATTGCTGTTGATGCACAAAATGATGCA
GCAAAGCAAACAAATGATGTTGAAGATTTAATTCAAAAAGGTGTCGATGTAGTTGTTATTAATCCAACTGATTCAGATGC
TGTTGCTTCAGCAGTAAGTGCAGCAAATGCAGCGAATGTTCCTGTTATTACAGTAGACCGCGTTGCAAATTCAGGTAAAG
TTGTTTCTCACATTGCCTCTAATAACATTGAAGGTGGTCAAATGGCTAGTGATTACGTTCGTGAATTAGTTGGCGAAGGA
GCAAACGTTGCTGAATTAGAAGGAATCCCTGGATCTTCTGCTGCTCGTGAGCGCGGGAAAGGATTCCATAACGTAGCGGA
TAAATCTTTAAAAGTAGTTGCTAAACAAGCGGCTGATTTTGATAGAGCAAAAGGATTATCTGTTATGGAAAACATTTTGC
AAGCGAATAGCGACATTAAAGCCGTGTTTGCGCACAACGACGAAATGGCATTAGGGGCACTTGAAGCATTGAAGTCTGCT
GGAAAAACGGATGTAGTCGTTGTTGGATTTGATGCAACAGAAGATGCTGTGCAAGCGGTTAACGATGGGCGTATGGCTGC
AACAGTTGCTCAAAAACCGGAATTAATCGGAGAGAAGGCGATGCAAACAGCAAAAGAGATCACGCAAGGTAAAAAAGTGG
ACAAATCTATTCCGATTGAATTAGAGCTTATTAAGAAAAATAAATAA

Upstream 100 bases:

>100_bases
TAGGTGTATCTTCTTTCTTCCAACAAGTTGTAAAAGGACTTGTAATCTTACTAGCTGTATTAATTGATCGCCGAAAAGAA
GCGTAATGGAGGGACAGTTC

Downstream 100 bases:

>100_bases
ATATAAAATGAAAATTAGGAAGGAAGAATAACAAATGAAATTCTTTATTGATACAGCAAACATTAACGAAATTAAAGAGG
CAAATGCATTAGGCGTATTA

Product: ribose ABC transporter substrate-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKKWLLIFVACIMVITAGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGAEKKAKDSGIELIAVDAQNDA
AKQTNDVEDLIQKGVDVVVINPTDSDAVASAVSAANAANVPVITVDRVANSGKVVSHIASNNIEGGQMASDYVRELVGEG
ANVAELEGIPGSSAARERGKGFHNVADKSLKVVAKQAADFDRAKGLSVMENILQANSDIKAVFAHNDEMALGALEALKSA
GKTDVVVVGFDATEDAVQAVNDGRMAATVAQKPELIGEKAMQTAKEITQGKKVDKSIPIELELIKKNK

Sequences:

>Translated_308_residues
MKKWLLIFVACIMVITAGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGAEKKAKDSGIELIAVDAQNDA
AKQTNDVEDLIQKGVDVVVINPTDSDAVASAVSAANAANVPVITVDRVANSGKVVSHIASNNIEGGQMASDYVRELVGEG
ANVAELEGIPGSSAARERGKGFHNVADKSLKVVAKQAADFDRAKGLSVMENILQANSDIKAVFAHNDEMALGALEALKSA
GKTDVVVVGFDATEDAVQAVNDGRMAATVAQKPELIGEKAMQTAKEITQGKKVDKSIPIELELIKKNK
>Mature_308_residues
MKKWLLIFVACIMVITAGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGAEKKAKDSGIELIAVDAQNDA
AKQTNDVEDLIQKGVDVVVINPTDSDAVASAVSAANAANVPVITVDRVANSGKVVSHIASNNIEGGQMASDYVRELVGEG
ANVAELEGIPGSSAARERGKGFHNVADKSLKVVAKQAADFDRAKGLSVMENILQANSDIKAVFAHNDEMALGALEALKSA
GKTDVVVVGFDATEDAVQAVNDGRMAATVAQKPELIGEKAMQTAKEITQGKKVDKSIPIELELIKKNK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=267, Percent_Identity=56.5543071161049, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1790526, Length=269, Percent_Identity=34.2007434944238, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790674, Length=258, Percent_Identity=31.3953488372093, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788473, Length=273, Percent_Identity=27.1062271062271, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790194, Length=228, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789990, Length=264, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786540, Length=225, Percent_Identity=28.4444444444444, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787948, Length=282, Percent_Identity=24.468085106383, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32501; Mature: 32501

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLIFVACIMVITAGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEEECCC
EKKAKDSGIELIAVDAQNDAAKQTNDVEDLIQKGVDVVVINPTDSDAVASAVSAANAANV
CHHHHCCCCEEEEEECCCCCHHCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCC
PVITVDRVANSGKVVSHIASNNIEGGQMASDYVRELVGEGANVAELEGIPGSSAARERGK
CEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCC
GFHNVADKSLKVVAKQAADFDRAKGLSVMENILQANSDIKAVFAHNDEMALGALEALKSA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHC
GKTDVVVVGFDATEDAVQAVNDGRMAATVAQKPELIGEKAMQTAKEITQGKKVDKSIPIE
CCCCEEEEECCCCHHHHHHHCCCCEEEHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LELIKKNK
EEEEECCC
>Mature Secondary Structure
MKKWLLIFVACIMVITAGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEEECCC
EKKAKDSGIELIAVDAQNDAAKQTNDVEDLIQKGVDVVVINPTDSDAVASAVSAANAANV
CHHHHCCCCEEEEEECCCCCHHCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCC
PVITVDRVANSGKVVSHIASNNIEGGQMASDYVRELVGEGANVAELEGIPGSSAARERGK
CEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCC
GFHNVADKSLKVVAKQAADFDRAKGLSVMENILQANSDIKAVFAHNDEMALGALEALKSA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHC
GKTDVVVVGFDATEDAVQAVNDGRMAATVAQKPELIGEKAMQTAKEITQGKKVDKSIPIE
CCCCEEEEECCCCHHHHHHHCCCCEEEHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LELIKKNK
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]