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The map label for this gene is dsbA

Identifier: 22127683

GI number: 22127683

Start: 4236383

End: 4237006

Strand: Reverse

Name: dsbA

Synonym: y3812

Alternate gene names: 22127683

Gene position: 4237006-4236383 (Counterclockwise)

Preceding gene: 22127684

Following gene: 22127682

Centisome position: 92.09

GC content: 42.15

Gene sequence:

>624_bases
ATGAAAAATGTATGGTTAGCACTCGTTGGTATCGTGATGGCGTTCAGTGTGACGGCTGCACAATTCACCGATGGGAAGCA
GTATCTAACGTTAGATAAGCCAGTAACGGGTGAACCTCAGGTTCTAGAGTTTTTCTCTTTCTATTGCCCACATTGTTACC
AGTTTGAAGAGGTTTATCAGGTACCTAAAGCAGTAAAAAAAGCGCTACCTGAAGGGACAAAAATGACCCGTTACCACGTT
GAATTTCTTGGTCCTCTCGGCAAGCAACTGACGCAAGCGTGGGCTGTCGCTATGGCATTGGGGGTTGAAGAAAAAATTAC
CCCACTGATGTTTGAAGGTGTGCAGAAGACACAAACTGTACAGACTCCTGGCGATATTCGTAATGTCTTTATCAAGGCGG
GTATCAGTGGTGAAGACTACGACGCTGCATTGAATAGTTTTGTGGTTAAATCATTAGTTGCTCAGCAACAAAAAGCGGCT
GAAGATTTACAACTACGTGGTGTTCCTGCGATGTTTGTTAATGGAAAATATATGATTAAGAATGATGGTATGGATACCAG
CTCAATGGATAATTATGTTAAACAGTATGCGGATGTTGTTACATTCCTGCTGACTCAAAAATAA

Upstream 100 bases:

>100_bases
GCAGACAGCATCATTCACTGAGCAGGTTAAGCTGTTGCAGGAACCCCCTTTGCAGCTGATGCCAATGTACTAAAGCTAAG
ATAATGGAGATTGTTATAGT

Downstream 100 bases:

>100_bases
TTTAGTACATAAAGAGGGGGCTTTTGTTGCCCCTTTTTTTTATTTATTTTTATGCTGTTTAGCTTGGGTTTATCTATTTT
AGGGAGCAATAGATATCAAT

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKNVWLALVGIVMAFSVTAAQFTDGKQYLTLDKPVTGEPQVLEFFSFYCPHCYQFEEVYQVPKAVKKALPEGTKMTRYHV
EFLGPLGKQLTQAWAVAMALGVEEKITPLMFEGVQKTQTVQTPGDIRNVFIKAGISGEDYDAALNSFVVKSLVAQQQKAA
EDLQLRGVPAMFVNGKYMIKNDGMDTSSMDNYVKQYADVVTFLLTQK

Sequences:

>Translated_207_residues
MKNVWLALVGIVMAFSVTAAQFTDGKQYLTLDKPVTGEPQVLEFFSFYCPHCYQFEEVYQVPKAVKKALPEGTKMTRYHV
EFLGPLGKQLTQAWAVAMALGVEEKITPLMFEGVQKTQTVQTPGDIRNVFIKAGISGEDYDAALNSFVVKSLVAQQQKAA
EDLQLRGVPAMFVNGKYMIKNDGMDTSSMDNYVKQYADVVTFLLTQK
>Mature_207_residues
MKNVWLALVGIVMAFSVTAAQFTDGKQYLTLDKPVTGEPQVLEFFSFYCPHCYQFEEVYQVPKAVKKALPEGTKMTRYHV
EFLGPLGKQLTQAWAVAMALGVEEKITPLMFEGVQKTQTVQTPGDIRNVFIKAGISGEDYDAALNSFVVKSLVAQQQKAA
EDLQLRGVPAMFVNGKYMIKNDGMDTSSMDNYVKQYADVVTFLLTQK

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1790291, Length=208, Percent_Identity=70.6730769230769, Blast_Score=311, Evalue=3e-86,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DSBA_YERPE (Q9XBV2)

Other databases:

- EMBL:   AF155130
- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AI0002
- RefSeq:   NP_671106.1
- RefSeq:   NP_991421.1
- RefSeq:   YP_002345112.1
- ProteinModelPortal:   Q9XBV2
- SMR:   Q9XBV2
- GeneID:   1148759
- GeneID:   1172865
- GeneID:   2764564
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0015
- KEGG:   ypk:y3812
- KEGG:   ypm:YP_0017
- HOGENOM:   HBG680642
- OMA:   CYQFEQV
- ProtClustDB:   PRK10954
- BioCyc:   YPES187410:Y3812-MONOMER
- BioCyc:   YPES214092:YPO0015-MONOMER
- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PIRSF:   PIRSF001488

Pfam domain/function: PF01323 DSBA; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 23100; Mature: 23100

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVWLALVGIVMAFSVTAAQFTDGKQYLTLDKPVTGEPQVLEFFSFYCPHCYQFEEVYQ
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
VPKAVKKALPEGTKMTRYHVEFLGPLGKQLTQAWAVAMALGVEEKITPLMFEGVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
QTPGDIRNVFIKAGISGEDYDAALNSFVVKSLVAQQQKAAEDLQLRGVPAMFVNGKYMIK
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEE
NDGMDTSSMDNYVKQYADVVTFLLTQK
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNVWLALVGIVMAFSVTAAQFTDGKQYLTLDKPVTGEPQVLEFFSFYCPHCYQFEEVYQ
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
VPKAVKKALPEGTKMTRYHVEFLGPLGKQLTQAWAVAMALGVEEKITPLMFEGVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
QTPGDIRNVFIKAGISGEDYDAALNSFVVKSLVAQQQKAAEDLQLRGVPAMFVNGKYMIK
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEE
NDGMDTSSMDNYVKQYADVVTFLLTQK
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10438793; 11586360; 12142430