Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 22127505

GI number: 22127505

Start: 4025413

End: 4026900

Strand: Reverse

Name: murE [H]

Synonym: y3631

Alternate gene names: 22127505

Gene position: 4026900-4025413 (Counterclockwise)

Preceding gene: 22127506

Following gene: 22127504

Centisome position: 87.53

GC content: 53.76

Gene sequence:

>1488_bases
GTGGCAGATCGTAATTTGCGCGACTTATTCGCTCCTTGGGGGCTAGACGTCCCGGAGCGGGCGCTACGGGAAATGACATT
AGACAGCCGTATTGCAGCTGCCGGCGATTTATTTGTTGCGGTTGTTGGCCACCAGACGGATGGGCGTCGCTACATCCCGC
AAGCTATCGCCCAGGGTGTGGCTGCAATTGTGGCCGAAGCCGATGGTGTCGCACCGGATGTCAGCGTTGCCGAGATACAT
GGCGTCCCTGTTATTTATTTGCGTAATTTGAATCAGCACTTATCCACGCTGGCTGGGCAGTTTTACCATCAGCCTGGCGC
CGCATTGCGTTTGGTCGGTGTAACCGGAACGAATGGCAAAACCACCACTACGCAATTGTTAGCACAATGGAGCCAGGCGC
TGGGCGAAACCAGTGCAGTCATGGGCACCGTAGGTAATGGTTTATTAGGGCAGGTGATACCCACGGAGAACACCACCGGT
TCTGCCGTAGATATTCAACATTTGCTGCGTAATCTGGTTGATCAAGGTGCGACTTTTGCCGCCATGGAAGTTTCTTCCCA
CGGTTTGATTCAAGACCGTGTTGCTGCGTTATCCTTTGCAGCCGTAGTGTTCACTAACTTAAGCCGCGATCATCTGGATT
ATCACGGCAATATGACGAGCTATGAGGCGGCCAAATGGCTGCTGTTCTCCACTCATCAGTCCGAACACAAAATTATCAAT
GCTGATGATGACGTTGGCCGCCGTTGGTTAAGCCAATTGCCGCAGGCTGTCGCCGTCAGCATGGCAGGCAATGTCCCCAA
AGGCTGGAATGGCCCGTGGTTATCTGCCAACAAGGTGATTTATCACGATAATGGTGCCAGCATCGCGTTTGATTCAAGCT
GGGGCGAAGGCGAGCTAGAAAGCCGTCTGATGGGCGCTTTCAACGTCAGTAACTTGTTGGTGGCATTAGCGACGTTACTG
GTTCAGGGGTATCCCATGGCGCAGTTGTTAGCTGCAGCGCCTCATCTGCAACCGGTTTGTGGGCGTATGGAAGTGTTTAA
TGCGCCGGGTAAACCAACCGTTGTGGTGGATTATGCCCACACGCCAGATGCATTAGAAAAAGCCTTGGCCGCCGCCCGTT
TACATTGCACCGGCAAGCTGTGGTGTGTGTTTGGCTGTGGCGGTGACCGTGATAAAGGTAAGCGCCCACTTATGGGCGGT
ATCGCAGAACAACTGGCTGACTGTGTCGTGGTGACCGATGATAACCCACGCAGTGAAGAGCCACAGGCGATTGTTGCTGA
CATTCTCAGTGGCTTGCTGGACGCGGGGCGTGTGCAGGCGATACATGGCCGTGCCGAAGCCGTAACCAGTGCCATTATGC
AAGCCAAAGAAGACGACGTTGTCCTGATTGCCGGTAAAGGCCATGAAGATTATCAATTAGTTGGCAATCGTCGCTTGGAT
TATTCAGACCGTGTCACCGTCGCGCGTTTGTTAGGGGGGCAGGCATGA

Upstream 100 bases:

>100_bases
GCCATCATGGGCGGCGTTTTACGCACCATGAACATTGAGCCAGATGCGTTACCCACCGGTGATAAAAGCGAATTAGTGAT
TAATACAAAAGAGGGTTCAG

Downstream 100 bases:

>100_bases
TTAAGGTCTCTCTGCGTTTTCTGGCTAGCTTACTTAATGCTGAATATATCGTTGCTGAATCAGTCAGCGATGATATTGAG
ATAACTGAAGTGACCATTGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MADRNLRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIH
GVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTG
SAVDIQHLLRNLVDQGATFAAMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIIN
ADDDVGRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLL
VQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGG
IAEQLADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLD
YSDRVTVARLLGGQA

Sequences:

>Translated_495_residues
MADRNLRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIH
GVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTG
SAVDIQHLLRNLVDQGATFAAMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIIN
ADDDVGRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLL
VQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGG
IAEQLADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLD
YSDRVTVARLLGGQA
>Mature_494_residues
ADRNLRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIHG
VPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGS
AVDIQHLLRNLVDQGATFAAMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINA
DDDVGRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLLV
QGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGI
AEQLADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDY
SDRVTVARLLGGQA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=492, Percent_Identity=77.8455284552846, Blast_Score=745, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52806; Mature: 52674

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRNLRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGV
CCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHH
AAIVAEADGVAPDVSVAEIHGVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGK
HHHHHHCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
TTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDIQHLLRNLVDQGATFA
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCCEE
AMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIIN
EEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCCCCCCEEC
ADDDVGRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELE
CCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHH
SRLMGAFNVSNLLVALATLLVQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCHHEECCCCCCEEEEEECC
TPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQLADCVVVTDDNPRSEE
CHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCC
PQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLD
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCC
YSDRVTVARLLGGQA
CCCHHHHHHHHCCCC
>Mature Secondary Structure 
ADRNLRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGV
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHH
AAIVAEADGVAPDVSVAEIHGVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGK
HHHHHHCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
TTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDIQHLLRNLVDQGATFA
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCCEE
AMEVSSHGLIQDRVAALSFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIIN
EEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCCCCCCEEC
ADDDVGRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELE
CCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHH
SRLMGAFNVSNLLVALATLLVQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCHHEECCCCCCEEEEEECC
TPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQLADCVVVTDDNPRSEE
CHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCC
PQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLD
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCC
YSDRVTVARLLGGQA
CCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]