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The map label for this gene is murF [H]

Identifier: 22127504

GI number: 22127504

Start: 4024040

End: 4025416

Strand: Reverse

Name: murF [H]

Synonym: y3630

Alternate gene names: 22127504

Gene position: 4025416-4024040 (Counterclockwise)

Preceding gene: 22127505

Following gene: 22127503

Centisome position: 87.49

GC content: 52.21

Gene sequence:

>1377_bases
ATGATTAAGGTCTCTCTGCGTTTTCTGGCTAGCTTACTTAATGCTGAATATATCGTTGCTGAATCAGTCAGCGATGATAT
TGAGATAACTGAAGTGACCATTGATACCCGTAAGGTCACTGCTGGTTGTCTGTTTGTTGCGCTGAAAGGCGAGCGTTTTG
ATGGGCATGATTTTGCCGAAGATGCTATTGCCGCAGGTGCTGGCGCTTTGCTGGTAAGTAAACGCTTACTGGTGGGGGGG
CCGCAGTTAGTTGTCAAAGACACGCGCTTGGCGTTAGGGCAATTTGCGGCTTGGGTTCGCCAGCAAGTTCCGGCTCGGAT
AGTGGCATTAACCGGTTCATCGGGTAAAACCTCGGTAAAAGAAATGGCGGCAGCGATTTTGCGCCAATGCGGCAATGTGC
TGTATACCGCCGGTAACTTTAATAACGATATCGGTGTCCCGCTAACATTGCTGCGCCTAACGGCCGAACATGATTTTGCT
GTTATTGAACTTGGTGCCAACCATGTTGGTGAAATTGCCTACACCACCGATTTAAGCCGTCCAGAAAGCGCTTTGGTGAA
TAATTTAGCCGCCGCTCATCTGGAGGGGTTTGGTTCGCTGGCCGGTGTGGCGCAAGCCAAAGGTGAGATCTTCGCTGGCT
TGCCTGCCAATGGCACGGCGATTATCAATGCCGACAGTAACGACTGGCCGCACTGGCAAGAAACGCTACATAACAAACGG
GTTTGGCGTTTCTCGCCCCACGCCGCAGAACATATTGATTTCTATGCCAGTGATGTGCGGATCACGCCTCAACTCACCTC
CTTCACTTTGCATTCACCGTTTGGCACCGTGGCTATCGAATTGCCTCTACCGGGGCAGCATAACATCGCCAATGCGTTAG
CGGCGGCAGCATTAGCCATGTCGGTTGGGGCGGATTTAGCCGCAGTACGCCAAGGGTTGGCGCAGTTACAAGCAGTACCT
GGCCGGTTATTCCCCGTCCCATTAGCGGAAGGCCAATTGCTGTTGGATGACAGCTATAACGCCAACGTGGGTTCCATGAT
TGCCGCCGCACAGGTCTTGGCCGAGATGCCGGGTTACCGGGTGATGGTGGTCGGTGATATGGCCGAGTTGGGCGAAACCG
CGGCAGATTGCCATCGTCAGGTTGGTGAAGCCGCGAAACTCGCAGGCGTCGATAAAGTATTAAGCGTTGGCACATTGAGC
CAGATACTGAGCAGTGCGAGCGGGAATGGCGAACACTTTCAGGATCAAAATACATTGAGCACCCGTGTAAACGCGTTGCT
GAACGAATATCCGGTTATCACTGTTTTAATTAAAGGTTCACGTAGCGCCGCTATGGAAAATGTCGTGCGTGCGTTACAGG
AGAAGGCATCATGTTAG

Upstream 100 bases:

>100_bases
TGATTGCCGGTAAAGGCCATGAAGATTATCAATTAGTTGGCAATCGTCGCTTGGATTATTCAGACCGTGTCACCGTCGCG
CGTTTGTTAGGGGGGCAGGC

Downstream 100 bases:

>100_bases
TTTGGTTGGCTGAATACTTAGTGAAATTTTACTCAGGCTTTAACGTCTTTTCCTATTTGACGTTCCGCGCCATCGTGAGT
CTATTAACCGCACTGTTTAT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MIKVSLRFLASLLNAEYIVAESVSDDIEITEVTIDTRKVTAGCLFVALKGERFDGHDFAEDAIAAGAGALLVSKRLLVGG
PQLVVKDTRLALGQFAAWVRQQVPARIVALTGSSGKTSVKEMAAAILRQCGNVLYTAGNFNNDIGVPLTLLRLTAEHDFA
VIELGANHVGEIAYTTDLSRPESALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPANGTAIINADSNDWPHWQETLHNKR
VWRFSPHAAEHIDFYASDVRITPQLTSFTLHSPFGTVAIELPLPGQHNIANALAAAALAMSVGADLAAVRQGLAQLQAVP
GRLFPVPLAEGQLLLDDSYNANVGSMIAAAQVLAEMPGYRVMVVGDMAELGETAADCHRQVGEAAKLAGVDKVLSVGTLS
QILSSASGNGEHFQDQNTLSTRVNALLNEYPVITVLIKGSRSAAMENVVRALQEKASC

Sequences:

>Translated_458_residues
MIKVSLRFLASLLNAEYIVAESVSDDIEITEVTIDTRKVTAGCLFVALKGERFDGHDFAEDAIAAGAGALLVSKRLLVGG
PQLVVKDTRLALGQFAAWVRQQVPARIVALTGSSGKTSVKEMAAAILRQCGNVLYTAGNFNNDIGVPLTLLRLTAEHDFA
VIELGANHVGEIAYTTDLSRPESALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPANGTAIINADSNDWPHWQETLHNKR
VWRFSPHAAEHIDFYASDVRITPQLTSFTLHSPFGTVAIELPLPGQHNIANALAAAALAMSVGADLAAVRQGLAQLQAVP
GRLFPVPLAEGQLLLDDSYNANVGSMIAAAQVLAEMPGYRVMVVGDMAELGETAADCHRQVGEAAKLAGVDKVLSVGTLS
QILSSASGNGEHFQDQNTLSTRVNALLNEYPVITVLIKGSRSAAMENVVRALQEKASC
>Mature_458_residues
MIKVSLRFLASLLNAEYIVAESVSDDIEITEVTIDTRKVTAGCLFVALKGERFDGHDFAEDAIAAGAGALLVSKRLLVGG
PQLVVKDTRLALGQFAAWVRQQVPARIVALTGSSGKTSVKEMAAAILRQCGNVLYTAGNFNNDIGVPLTLLRLTAEHDFA
VIELGANHVGEIAYTTDLSRPESALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPANGTAIINADSNDWPHWQETLHNKR
VWRFSPHAAEHIDFYASDVRITPQLTSFTLHSPFGTVAIELPLPGQHNIANALAAAALAMSVGADLAAVRQGLAQLQAVP
GRLFPVPLAEGQLLLDDSYNANVGSMIAAAQVLAEMPGYRVMVVGDMAELGETAADCHRQVGEAAKLAGVDKVLSVGTLS
QILSSASGNGEHFQDQNTLSTRVNALLNEYPVITVLIKGSRSAAMENVVRALQEKASC

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=458, Percent_Identity=69.8689956331878, Blast_Score=607, Evalue=1e-175,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48371; Mature: 48371

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVSLRFLASLLNAEYIVAESVSDDIEITEVTIDTRKVTAGCLFVALKGERFDGHDFAE
CEEEEHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCEEEEEEEEEEEECCCCCCCCHHH
DAIAAGAGALLVSKRLLVGGPQLVVKDTRLALGQFAAWVRQQVPARIVALTGSSGKTSVK
HHHHCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHH
EMAAAILRQCGNVLYTAGNFNNDIGVPLTLLRLTAEHDFAVIELGANHVGEIAYTTDLSR
HHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECCCCC
PESALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPANGTAIINADSNDWPHWQETLHNKR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCHHHHHHCCCC
VWRFSPHAAEHIDFYASDVRITPQLTSFTLHSPFGTVAIELPLPGQHNIANALAAAALAM
EEEECCCHHHHHHEEECCEEECCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
SVGADLAAVRQGLAQLQAVPGRLFPVPLAEGQLLLDDSYNANVGSMIAAAQVLAEMPGYR
HHCCCHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
VMVVGDMAELGETAADCHRQVGEAAKLAGVDKVLSVGTLSQILSSASGNGEHFQDQNTLS
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
TRVNALLNEYPVITVLIKGSRSAAMENVVRALQEKASC
HHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVSLRFLASLLNAEYIVAESVSDDIEITEVTIDTRKVTAGCLFVALKGERFDGHDFAE
CEEEEHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCEEEEEEEEEEEECCCCCCCCHHH
DAIAAGAGALLVSKRLLVGGPQLVVKDTRLALGQFAAWVRQQVPARIVALTGSSGKTSVK
HHHHCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHH
EMAAAILRQCGNVLYTAGNFNNDIGVPLTLLRLTAEHDFAVIELGANHVGEIAYTTDLSR
HHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECCCCC
PESALVNNLAAAHLEGFGSLAGVAQAKGEIFAGLPANGTAIINADSNDWPHWQETLHNKR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCHHHHHHCCCC
VWRFSPHAAEHIDFYASDVRITPQLTSFTLHSPFGTVAIELPLPGQHNIANALAAAALAM
EEEECCCHHHHHHEEECCEEECCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
SVGADLAAVRQGLAQLQAVPGRLFPVPLAEGQLLLDDSYNANVGSMIAAAQVLAEMPGYR
HHCCCHHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
VMVVGDMAELGETAADCHRQVGEAAKLAGVDKVLSVGTLSQILSSASGNGEHFQDQNTLS
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
TRVNALLNEYPVITVLIKGSRSAAMENVVRALQEKASC
HHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]