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The map label for this gene is hsrA [H]

Identifier: 22123932

GI number: 22123932

Start: 9262

End: 10686

Strand: Reverse

Name: hsrA [H]

Synonym: y0010

Alternate gene names: 22123932

Gene position: 10686-9262 (Counterclockwise)

Preceding gene: 22123933

Following gene: 22123927

Centisome position: 0.23

GC content: 46.46

Gene sequence:

>1425_bases
ATGATAAAATCTGCGCGCAGTATGGCAGGCCTTCCTTGGATAGCGGCTATGGCTTTCTTTATGCAGGCTTTGGATGCCAC
CATACTGAATACGGCTCTCCCTTCAATCGCCGAAAGCCTTAACCGTTCTCCGCTGACAATGCAATCAGCCATTATTAGCT
ATACCCTGACCGTCGCGATGTTGATTCCAGTTAGTGGTTGGCTGGCCGATCGTTTTGGTACCCGACGAATTTTTATCCTG
GCGGTTTCTTTATTTACCTTCGGATCACTGCTCTGTGCACTTTCAGGCTCACTGGCTTTCTTAGTGGCTTCTCGGGTTAT
TCAAGGGATCGGTGGGGCGATGATGATGCCGGTAGCCAGGCTGGCGCTCATCCGGGCTTATCCACGCAGTGAGTTATTGC
CGGTTTTAAATTTTGTCACTATTCCAGGGTTAGTCGGGCCAGTCATGGGGCCATTATTGGGCGGGTTGTTGGTGACTTAT
ACCACTTGGCATTGGATTTTTATCCTTAATATTCCTATTGGCTTGTTGGGTATTTTTTATGCCCGCAAATATATGCCTAA
TTTCACGATGCCTAAGCGCACTTTTGATTTTGTTGGTTTCCTACTATTTGGTATCAGTTTAGTCATGATATCCACCAGCC
TGGAGATTATGGGGCGGCCAGAAATCGCAGATTATTTACCCGCCGCAACTCTGCTAGGCGGCCTATTAATGCTGATTTTC
TATATATTTCATGCCAAAGGCCATCCTAATCCACTCATTGGTTTGCCATTATTTAAGACCCGGACATTCTCCGTGGGGAT
TGCCGGTAACGTGGCCTCCCGCTTAGGGACGGGCTGTGTACCTTTTTTAATGCCGTTGATGTTACAAGTTGGATTCGGTT
ATTCCGCACTTATCGCCGGTTGTATGATGGCTCCCACCGCGATTGGATCCATCATGGCAAAATCAGCCGTCACACAGGTG
CTGCGCTCACTGGGCTATCGTAAAGTGTTAGTCGGTATAACCTTAATTATCGGGGTACTGATTGCCTCCTTTGCATTGCA
ATCACCGGGGATGTCGGCATGGATGATGATATTGCCGCTATTTATATTAGGTATAGCGATGTCGACCCAATTTACTGCGA
TGAATACAATTACACTGGCTGATTTGACGGATAACAATGCCAGCTCCGGTAACAGTGTTCTGGCGGTAACCCAACAATTA
TCGATAAGCTTTGGGATTGCTATCAGTGCGACTGTGTTGCGTTTTTATGATGGGTTGTCGTTAGGCGGTAATGTGGATCA
CTTCCACTACACCTTTATCACCATGGGAATAGTGACATTGCTATCATCTCTGGTGTTCTTGTTACTCAAACCCAGGGATG
GAGACAATTTAATTCAGGGCCGTAATGTGAAAAAAGTAGCCCCACAGGTGAAAAATAATGTTTAA

Upstream 100 bases:

>100_bases
CTATTAAAAACGGTAGACTGACGACAGCACGATCAGCCACATGCTATCTATATAAAGAAGAGAAAATTATCGCCCCCCAT
ATAAATCACCAGGACGGATA

Downstream 100 bases:

>100_bases
CGAAGGGAGACCGAACCGCGTTCTATTAGCTCGGGAGTCAGGGGCAGAACTTGTGGCTCAGCTTCTGGATTATTTAGTCG
TTGCTTCAGGGCAACCAGGC

Product: major facilitator permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MIKSARSMAGLPWIAAMAFFMQALDATILNTALPSIAESLNRSPLTMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFIL
AVSLFTFGSLLCALSGSLAFLVASRVIQGIGGAMMMPVARLALIRAYPRSELLPVLNFVTIPGLVGPVMGPLLGGLLVTY
TTWHWIFILNIPIGLLGIFYARKYMPNFTMPKRTFDFVGFLLFGISLVMISTSLEIMGRPEIADYLPAATLLGGLLMLIF
YIFHAKGHPNPLIGLPLFKTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSALIAGCMMAPTAIGSIMAKSAVTQV
LRSLGYRKVLVGITLIIGVLIASFALQSPGMSAWMMILPLFILGIAMSTQFTAMNTITLADLTDNNASSGNSVLAVTQQL
SISFGIAISATVLRFYDGLSLGGNVDHFHYTFITMGIVTLLSSLVFLLLKPRDGDNLIQGRNVKKVAPQVKNNV

Sequences:

>Translated_474_residues
MIKSARSMAGLPWIAAMAFFMQALDATILNTALPSIAESLNRSPLTMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFIL
AVSLFTFGSLLCALSGSLAFLVASRVIQGIGGAMMMPVARLALIRAYPRSELLPVLNFVTIPGLVGPVMGPLLGGLLVTY
TTWHWIFILNIPIGLLGIFYARKYMPNFTMPKRTFDFVGFLLFGISLVMISTSLEIMGRPEIADYLPAATLLGGLLMLIF
YIFHAKGHPNPLIGLPLFKTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSALIAGCMMAPTAIGSIMAKSAVTQV
LRSLGYRKVLVGITLIIGVLIASFALQSPGMSAWMMILPLFILGIAMSTQFTAMNTITLADLTDNNASSGNSVLAVTQQL
SISFGIAISATVLRFYDGLSLGGNVDHFHYTFITMGIVTLLSSLVFLLLKPRDGDNLIQGRNVKKVAPQVKNNV
>Mature_474_residues
MIKSARSMAGLPWIAAMAFFMQALDATILNTALPSIAESLNRSPLTMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFIL
AVSLFTFGSLLCALSGSLAFLVASRVIQGIGGAMMMPVARLALIRAYPRSELLPVLNFVTIPGLVGPVMGPLLGGLLVTY
TTWHWIFILNIPIGLLGIFYARKYMPNFTMPKRTFDFVGFLLFGISLVMISTSLEIMGRPEIADYLPAATLLGGLLMLIF
YIFHAKGHPNPLIGLPLFKTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSALIAGCMMAPTAIGSIMAKSAVTQV
LRSLGYRKVLVGITLIIGVLIASFALQSPGMSAWMMILPLFILGIAMSTQFTAMNTITLADLTDNNASSGNSVLAVTQQL
SISFGIAISATVLRFYDGLSLGGNVDHFHYTFITMGIVTLLSSLVFLLLKPRDGDNLIQGRNVKKVAPQVKNNV

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=463, Percent_Identity=73.4341252699784, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=459, Percent_Identity=43.7908496732026, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1789042, Length=471, Percent_Identity=28.8747346072187, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788710, Length=427, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87081983, Length=288, Percent_Identity=29.8611111111111, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322958, Length=492, Percent_Identity=25.8130081300813, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=31.2925170068027, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=31.496062992126, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50936; Mature: 50936

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSARSMAGLPWIAAMAFFMQALDATILNTALPSIAESLNRSPLTMQSAIISYTLTVAM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LIPVSGWLADRFGTRRIFILAVSLFTFGSLLCALSGSLAFLVASRVIQGIGGAMMMPVAR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHH
LALIRAYPRSELLPVLNFVTIPGLVGPVMGPLLGGLLVTYTTWHWIFILNIPIGLLGIFY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHH
ARKYMPNFTMPKRTFDFVGFLLFGISLVMISTSLEIMGRPEIADYLPAATLLGGLLMLIF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHH
YIFHAKGHPNPLIGLPLFKTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSALIAG
HHHHCCCCCCCEECCCCEECEEEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
CMMAPTAIGSIMAKSAVTQVLRSLGYRKVLVGITLIIGVLIASFALQSPGMSAWMMILPL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FILGIAMSTQFTAMNTITLADLTDNNASSGNSVLAVTQQLSISFGIAISATVLRFYDGLS
HHHHHHHHCCEEHEEEEEEEEECCCCCCCCCCEEEEHHHHHHHCCHHHHHHHHHHHCCCC
LGGNVDHFHYTFITMGIVTLLSSLVFLLLKPRDGDNLIQGRNVKKVAPQVKNNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHCCC
>Mature Secondary Structure
MIKSARSMAGLPWIAAMAFFMQALDATILNTALPSIAESLNRSPLTMQSAIISYTLTVAM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LIPVSGWLADRFGTRRIFILAVSLFTFGSLLCALSGSLAFLVASRVIQGIGGAMMMPVAR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHH
LALIRAYPRSELLPVLNFVTIPGLVGPVMGPLLGGLLVTYTTWHWIFILNIPIGLLGIFY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHH
ARKYMPNFTMPKRTFDFVGFLLFGISLVMISTSLEIMGRPEIADYLPAATLLGGLLMLIF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHH
YIFHAKGHPNPLIGLPLFKTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSALIAG
HHHHCCCCCCCEECCCCEECEEEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
CMMAPTAIGSIMAKSAVTQVLRSLGYRKVLVGITLIIGVLIASFALQSPGMSAWMMILPL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FILGIAMSTQFTAMNTITLADLTDNNASSGNSVLAVTQQLSISFGIAISATVLRFYDGLS
HHHHHHHHCCEEHEEEEEEEEECCCCCCCCCCEEEEHHHHHHHCCHHHHHHHHHHHCCCC
LGGNVDHFHYTFITMGIVTLLSSLVFLLLKPRDGDNLIQGRNVKKVAPQVKNNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]