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The map label for this gene is gltX [H]

Identifier: 221232783

GI number: 221232783

Start: 2034124

End: 2035584

Strand: Reverse

Name: gltX [H]

Synonym: SPN23F_20940

Alternate gene names: 221232783

Gene position: 2035584-2034124 (Counterclockwise)

Preceding gene: 221232784

Following gene: 221232782

Centisome position: 91.64

GC content: 40.59

Gene sequence:

>1461_bases
ATGTCAAAAGATATCCGCGTACGTTACGCACCAAGTCCAACAGGACTACTACACATCGGAAATGCCCGTACAGCATTGTT
TAACTACTTGTATGCGCGCCATCATGGTGGAACATTTCTCATCCGTATCGAAGATACTGACCGTAAACGCCATGTCGAGG
ATGGTGAACGTTCACAACTTGAAAACCTTCGCTGGTTAGGCATGGATTGGGATGAAAGTCCAGAATCACATGAGAATTAT
CGCCAGTCTGAGCGTTTGGACTTGTATCAAAAATACATTGACCAACTATTAGCTGAAGGAAAAGCCTATAAATCTTACGT
TACAGAAGAAGAGTTGGCAGCTGAACGTGAACGTCAAGAAGTAGCTGGCGAAACACCACGCTACATCAATGAATACCTTG
GTATGAGTGAAGAAGAAAAAGCGGCTTATATCGCAGAACGTGAAGCAGCTGGTATCATACCAACAGTTCGTTTGGCTGTC
AATGAGTCAGGTATCTACAAGTGGCATGATATGGTCAAAGGTGATATCGAATTTGAAGGTGGCAATATCGGCGGTGACTG
GGTTATCCAAAAGAAAGACGGTTACCCAACTTACAACTTTGCCGTTGTTATCGATGACCACGATATGCAAATCTCTCATG
TTATCCGTGGAGATGACCATATTGCTAATACACCAAAACAGCTTATGGTCTATGAAGCTCTTGGTTGGGAAGCTCCAGAG
TTTGGTCACATGACCTTGATTATCAACTCTGAGACTGGTAAGAAGTTATCTAAACGTGATACTAATACACTTCAATTTAT
CGAAGATTACCGAAAGAAAGGTTACCTTCCAGAAGCAGTCTTTAACTTTATTGCTCTTCTTGGTTGGAACCCAGGTGGTG
AAGATGAGATTTTCTCTCGTGAAGAACTCATTAAACTTTTTGATGAAAACCGCCTCAGCAAGTCACCAGCAGCCTTTGAT
CAGAAAAAATTAGACTGGATGAGCAATGATTATATCAAGAATGCAGACCTTGAAACCATCTTTGAAATGGCAAAACCATT
CTTAGAGGAAGCAGGCCGTTTGACTGACAAGGCTGAAAAATTAGTTGAGCTCTATAAACCACAAATGAAATCAGTAGATG
AGATTATCCCATTGACAGATCTTTTCTTCTCAGATTTCCCAGAATTGACAGAAGCAGAGCGCGAAGTCATGACGGGTGAA
ACAGTTCCAACAGTTCTTGAAGCATTCAAAGCAAAACTTGAAGCGATGACAGATGATGAATTTGTGACAGAAAATATCTT
CCCACAAATTAAAGCAGTCCAAAAAGAAACAGGTATTAAAGGGAAAAATCTTTTCATGCCTATTCGTATCGCAGTTTCAG
GCGAAATGCATGGGCCAGAATTACCAGATACAATTTTCTTGCTTGGACGTGAAAAATCAATTCAGCATATCGAAAACATT
CTAAAAGAAATCTCTAAATAA

Upstream 100 bases:

>100_bases
ACTCTTTTTACTCTCTTTATCCATAGAAATTGGACTCAGCCAAGACTTGTGATATAATATAGAAAGCAAAAAGGCAGACG
CCTAGATAATAGGAGAAACT

Downstream 100 bases:

>100_bases
AGAGGATACAATAATGGACATCTGGGAAAAGATGTACGAAGAAGCACAGAAACTATACAATCCACATGAAGTATCAGACT
TTGTTTATGCCAATCATGTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQLENLRWLGMDWDESPESHENY
RQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFD
QKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGE
TVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENI
LKEISK

Sequences:

>Translated_486_residues
MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQLENLRWLGMDWDESPESHENY
RQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFD
QKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGE
TVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENI
LKEISK
>Mature_485_residues
SKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYR
QSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAVN
ESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPEF
GHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQ
KKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGET
VPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENIL
KEISK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=29.9196787148594, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI62241042, Length=258, Percent_Identity=26.7441860465116, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4826960, Length=298, Percent_Identity=24.8322147651007, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788743, Length=485, Percent_Identity=38.3505154639175, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=30.0395256916996, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1786895, Length=217, Percent_Identity=25.8064516129032, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=503, Percent_Identity=30.8151093439364, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17543332, Length=298, Percent_Identity=25.8389261744966, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=496, Percent_Identity=32.6612903225806, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=218, Percent_Identity=27.5229357798165, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=310, Percent_Identity=24.1935483870968, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=30.4174950298211, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17864488, Length=300, Percent_Identity=27.6666666666667, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55910; Mature: 55779

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQL
CCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQE
HCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
VAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAVNESGIYKWHDMVKGDIEFEG
HCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCEEEECC
GNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCHHHHHEEHHHCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHCH
EELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGETVPTVLEAFKAKLEAMTDDE
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
FVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHH
LKEISK
HHHHCC
>Mature Secondary Structure 
SKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQL
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQE
HCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
VAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAVNESGIYKWHDMVKGDIEFEG
HCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCEEEECC
GNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCHHHHHEEHHHCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAVFNFIALLGWNPGGEDEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHCH
EELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKNADLETIFEMAKPFLEEAGRLTDKAEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGETVPTVLEAFKAKLEAMTDDE
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
FVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHH
LKEISK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA