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The map label for this gene is purR

Identifier: 221232703

GI number: 221232703

Start: 1940797

End: 1941624

Strand: Reverse

Name: purR

Synonym: SPN23F_19990

Alternate gene names: 221232703

Gene position: 1941624-1940797 (Counterclockwise)

Preceding gene: 221232704

Following gene: 221232702

Centisome position: 87.41

GC content: 42.15

Gene sequence:

>828_bases
ATGAAATTAAGAAGAAGTGATCGGATGGTTGTCATTTCCAACTATTTGATTAATAATCCTTATAAACTAACTAGTCTCAA
TACTTTTGCTGAAAAGTATGAGTCTGCTAAATCATCCATCTCAGAAGATATCGTCATTATCAAACGCGCCTTTGAGGAAA
TTGAAATCGGTCATATCCAGACAGTGACTGGGGCTGGCGGAGGTGTCATCTTCACACCGTCTATTTCGAGTCAGGATGCT
AAGGAAATGGTTGAAGACTTGCGTACCAAGTTGTCAGAAAGTGACCGTATCTTGCCAGGTGGTTATATCTATCTGTCTGA
TTTGCTTAGCACACCAGCCATCTTGAAAAATATTGGTCGTATTATTGCCAAAAGCTTTATGGACCAAAAAATTGACGCGG
TTATGACCGTAGCAACTAAGGGTGTGCCACTTGCAAATGCAGTTGCCAATGTCCTCAATGTCTCTTTTGTCATTGTGCGC
CGTGACCTGAAAATTACCGAAGGTTCAACTGTTAGCGTCAACTATGTTTCAGGTTCAAGTGGTGACCGTATCGAGAAAAT
GTTCCTTTCAAAACGTAGTCTTAAGGCAGGTAGCCGTGTCTTGATTGTGGATGACTTCTTGAAAGGTGGCGGAACGGTCA
ACGGTATGATTAGTCTCTTGCGCGAGTTCGACTCAGAACTGGCAGGTGTAGCGGTCTTTGCGGACAATGCCCAAGAAGAA
CGTGAAAAGCAGTTTGACTACAAGTCACTCTTGAAGGTAACTAATATTGATGTCAAGAACCAAGCCATCGATGTTGAGGT
TGGCAATATCTTTGACGAAGATAAATAA

Upstream 100 bases:

>100_bases
AGTTCTATTGACAATCTCAAAACAGTGTTTTGAACCACCAGCGACCAGCTTTCTAGTTTGCTTTTTGATTTTTTGAATAA
AAATGGAATAGGAAATAGAA

Downstream 100 bases:

>100_bases
GAGATAGAACTAAAGGTTGGAACGATTGTCCCAGCCTTTCTTTGCAAACAGAATAGAAGGAAGCTTATGAAAACACCATT
TATCAATAGAGAAGAGTTAG

Product: pur operon repressor

Products: AMP; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDIVIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDA
KEMVEDLRTKLSESDRILPGGYIYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMISLLREFDSELAGVAVFADNAQEE
REKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK

Sequences:

>Translated_275_residues
MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDIVIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDA
KEMVEDLRTKLSESDRILPGGYIYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMISLLREFDSELAGVAVFADNAQEE
REKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK
>Mature_275_residues
MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDIVIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDA
KEMVEDLRTKLSESDRILPGGYIYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMISLLREFDSELAGVAVFADNAQEE
REKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK

Specific function: Controls the transcription of the pur operon for purine biosynthetic genes

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_STRPN (P65832)

Other databases:

- EMBL:   AE005672
- PIR:   E95231
- RefSeq:   NP_346406.1
- ProteinModelPortal:   P65832
- SMR:   P65832
- EnsemblBacteria:   EBSTRT00000025159
- GeneID:   929904
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1979
- TIGR:   SP_1979
- GeneTree:   EBGT00050000026781
- HOGENOM:   HBG304155
- OMA:   EQQGIGT
- ProtClustDB:   PRK09213
- BioCyc:   SPNE170187-1:SP_1979-MONOMER
- GO:   GO:0006350
- InterPro:   IPR000836
- InterPro:   IPR015265
- InterPro:   IPR010078
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD029816
- TIGRFAMs:   TIGR01743

Pfam domain/function: PF00156 Pribosyltran; PF09182 PuR_N

EC number: 2.4.2.7 [C]

Molecular weight: Translated: 30227; Mature: 30227

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDIVIIKRAFEEIEIGHIQ
CCCCCCCCEEEEEHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGYIYLSDLLSTPAILKNIGR
EEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
IIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVRRDLKITEGSTVSVNYVSGSS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEECEEEECCCEEEEEEEECCC
GDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMISLLREFDSELAGVAVFADNAQEE
CHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHH
REKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK
HHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDIVIIKRAFEEIEIGHIQ
CCCCCCCCEEEEEHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGYIYLSDLLSTPAILKNIGR
EEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
IIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVRRDLKITEGSTVSVNYVSGSS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEECEEEECCCEEEEEEEECCC
GDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMISLLREFDSELAGVAVFADNAQEE
CHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHH
REKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK
HHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 560 [C]

Specific activity: 14

Km value (mM): 0.02 {adenine}} 0.125 {5-phospho-alpha-D-ribose} [C]

Substrates: Adenine; 5-Phospho-alpha-D-ribose 1-diphosphate [C]

Specific reaction: Adenine + 5-Phospho-alpha-D-ribose 1-diphosphate --> AMP + Diphosphate [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916