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The map label for this gene is truA

Identifier: 221232354

GI number: 221232354

Start: 1554161

End: 1554910

Strand: Reverse

Name: truA

Synonym: SPN23F_16120

Alternate gene names: 221232354

Gene position: 1554910-1554161 (Counterclockwise)

Preceding gene: 221232355

Following gene: 221232353

Centisome position: 70.0

GC content: 43.73

Gene sequence:

>750_bases
ATGACGAGATATAAAGCAACTATTTCCTATGATGGTTATGCCTTTGCTGGCTTTCAGCGCCAGTCTCATGCGCGTAGCGT
TCAGGAAGAAATTGAAAAAACCTTGACCAGATTAAATAAAGGGCAAACCATTACTGTTCACGGTGCTGGTAGGACAGATA
GTGGGGTTCATGCCCTGGGACAGGTCATTCATTTTGACCTGCCTTATCAGATGGATGAGGAGAAACTCCGTTTTGCCTTG
GATACCCAGTCTCCTGAAGATATTGATGTGATTTCGATTGAGCTTGTGGCAGATGATTTTCATTGCCGTTATGCCAAACA
TAGCAAGACCTATGAGTTTATTGTGGATAGAGGACGTCCCAAAAATCCTATGCGCCGTCACTATGCCACCCACTTTCCCT
ACCCACTCGATGTGGAACGAATGCAGATTGCAATCAAAAAGCTAGAGGGAACCCATGATTTTACCGGTTTTACAGCCTCT
GGGACTAGTGTAGAGGATAAGGTTCGCACCATCACAGAAGCTAGTTTAATAGTCGATGAGACAGGACAATTTTTGACCTT
TACCTTTTCAGGAAATGGTTTCTTGTATAAACAGATTCGCAATATGGTGGGGACTCTTCTCAAAATCGGTAACAACCGCA
TGCCAGTAGAGCAGATTGATCTCATCTTGGAGAAGAAGGACAGGCAACTTGCAGGTCCCACTGCAGCACCAAATGGTTTG
TATTTAAAGGAGATTCGTTATGAAGAATAA

Upstream 100 bases:

>100_bases
TGGTTTGAAGAGATTTTCGAAGAGTATGAGATTTTTATACCCATACAATATTTGTTTTAGGTCCTTGTTTACAGACTAAT
TTCTTGATAAAATAGAGGTT

Downstream 100 bases:

>100_bases
TCGTATTTTAGCACTTTCTGGAAATGATATTTTTAGTGGTGGTGGACTGTCAGCTGATTTGGCTACCTATACCTTGAACG
GCTTGCATGGGTTTGTAGCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFAL
DTQSPEDIDVISIELVADDFHCRYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
YLKEIRYEE

Sequences:

>Translated_249_residues
MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFAL
DTQSPEDIDVISIELVADDFHCRYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
YLKEIRYEE
>Mature_248_residues
TRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALD
TQSPEDIDVISIELVADDFHCRYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASG
TSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGLY
LKEIRYEE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=31.5018315018315, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI199559785, Length=278, Percent_Identity=27.6978417266187, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=36.3636363636364, Blast_Score=164, Evalue=7e-42,
Organism=Drosophila melanogaster, GI116007752, Length=312, Percent_Identity=27.5641025641026, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=24.7104247104247, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=24.7104247104247, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_STRP2 (Q04JF7)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816881.1
- ProteinModelPortal:   Q04JF7
- SMR:   Q04JF7
- STRING:   Q04JF7
- EnsemblBacteria:   EBSTRT00000019055
- GeneID:   4442238
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1424
- eggNOG:   COG0101
- GeneTree:   EBGT00050000026796
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28413; Mature: 28281

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALG
CCEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
QVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHCRYAKHSKTYEFIVDRGRP
HHHEECCCCCCCHHHEEEEECCCCCCCCEEEEEEEEECCCCEEECCCCCCEEEEEECCCC
KNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASGTSVEDKVRTITEASLIVDE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHEEEEC
TGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCE
YLKEIRYEE
EEEEEECCC
>Mature Secondary Structure 
TRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALG
CEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
QVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHCRYAKHSKTYEFIVDRGRP
HHHEECCCCCCCHHHEEEEECCCCCCCCEEEEEEEEECCCCEEECCCCCCEEEEEECCCC
KNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASGTSVEDKVRTITEASLIVDE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHEEEEC
TGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCE
YLKEIRYEE
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA