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The map label for this gene is thiD [H]

Identifier: 221232353

GI number: 221232353

Start: 1553398

End: 1554171

Strand: Reverse

Name: thiD [H]

Synonym: SPN23F_16110

Alternate gene names: 221232353

Gene position: 1554171-1553398 (Counterclockwise)

Preceding gene: 221232354

Following gene: 221232352

Centisome position: 69.97

GC content: 42.25

Gene sequence:

>774_bases
ATGAAGAATAATCGTATTTTAGCACTTTCTGGAAATGATATTTTTAGTGGTGGTGGACTGTCAGCTGATTTGGCTACCTA
TACCTTGAACGGCTTGCATGGGTTTGTAGCAGTGACTTGTTTGACAGCCTTGACAGAAAAAGGATTTGAAGTCTTTCCAA
CTGATGATACCATTTTTCAACATGAATTAGATAGCTTACGTGATGTGGAATTTGGGGGAATTAAGATTGGTCTTCTCCCT
ACTGTCAGTGTGGCTGAGAAGGCCTTGGACTTTATCAAACAACGCCCAGGAGTACCTGTGGTGTTGGATCCTGTCTTGGT
CTGCAAGGAAACGCATGATGTAGCTGTCAGTGAGCTCTGCCAAGAGTTGATTCGCTTTTTCCCTTATGTCAGTGTGATTA
CGCCTAATCTCCCAGAAGCAGAATTATTATCCGGTCAGGAAATTAAAACCTTGGAAGACATGAAAACTGCAGCGCAGAAA
TTGCATGATTTAGGAGCGCCAGCAGTCATTATCAAGGGAGGCAATCGTCTTAGTCAGGACAAGGCTGTGGATGTCTTTTA
TGATGGACAGACCTTTACTATCCTAGAAAATCCAGTTATCCAAGGCCAAAATGCTGGTGCAGGTTGTACCTTTGCCTCTA
GCATTGCCAGTCACTTGATTAAAGGTGATAAACTTTTGCCAGCAGTAGAAAGCTCTAAGGCTTTCGTTTATCGTGCTATT
GCACAAGCAGATCAGTATGGAGTAAGACAATATGAAGCAAACAAAAACAACTAA

Upstream 100 bases:

>100_bases
ATGCCAGTAGAGCAGATTGATCTCATCTTGGAGAAGAAGGACAGGCAACTTGCAGGTCCCACTGCAGCACCAAATGGTTT
GTATTTAAAGGAGATTCGTT

Downstream 100 bases:

>100_bases
AATCGCCCTTGTATCCCTATTAACCGCCCTTTCTGTGGTTCTAGGTTATTTCTTAAAAATCCCAACACCTACAGGAATTC
TAACTCTTTTAGATGCTGGT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTALTEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLP
TVSVAEKALDFIKQRPGVPVVLDPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASHLIKGDKLLPAVESSKAFVYRAI
AQADQYGVRQYEANKNN

Sequences:

>Translated_257_residues
MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTALTEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLP
TVSVAEKALDFIKQRPGVPVVLDPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASHLIKGDKLLPAVESSKAFVYRAI
AQADQYGVRQYEANKNN
>Mature_257_residues
MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTALTEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLP
TVSVAEKALDFIKQRPGVPVVLDPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASHLIKGDKLLPAVESSKAFVYRAI
AQADQYGVRQYEANKNN

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=249, Percent_Identity=30.1204819277108, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325378, Length=252, Percent_Identity=28.968253968254, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324517, Length=255, Percent_Identity=31.3725490196078, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324997, Length=255, Percent_Identity=31.7647058823529, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 27726; Mature: 27726

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTALTEKGFEVFPTDDTIFQ
CCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
HELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVLDPVLVCKETHDVAVSELC
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCEEECCCHHHHHHHHH
QELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQKLHDLGAPAVIIKGGNRLSQD
HHHHHHCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
KAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASHLIKGDKLLPAVESSKAFVYRAI
CCEEEEECCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AQADQYGVRQYEANKNN
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTALTEKGFEVFPTDDTIFQ
CCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
HELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVLDPVLVCKETHDVAVSELC
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCEEECCCHHHHHHHHH
QELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQKLHDLGAPAVIIKGGNRLSQD
HHHHHHCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
KAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASHLIKGDKLLPAVESSKAFVYRAI
CCEEEEECCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AQADQYGVRQYEANKNN
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]