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The map label for this gene is grpE

Identifier: 221230826

GI number: 221230826

Start: 2970747

End: 2971436

Strand: Reverse

Name: grpE

Synonym: MLBr_02495

Alternate gene names: 221230826

Gene position: 2971436-2970747 (Counterclockwise)

Preceding gene: 221230827

Following gene: 221230825

Centisome position: 90.92

GC content: 60.43

Gene sequence:

>690_bases
GTGACCGAAGGCAATCCACACGAACAGGTGACGGTCACCGACAAACGACGGATCGATCCCGATACTGGCGAAGTACGATC
TATACCTCTCGGCGACACGCCGGGAGGTAGCGAGCCGGCTGGTATGACCGACCGAAGTGCCAACAGTACCGACACAATGG
TCGACAAAGTTGCTGAGCTGACTAGCGATCTGCAGCGGGTGCAGGCCGATTTTGCCAACTACCGTAAGCGAGCGTTGCGC
GACCAGCAGACGGCTTCGGATCGAGCCAAGGCCACTGTCATCAGCCAATTGTTGGGTGTGCTTGACGATTTCGATCGTGC
CCGCGAGCACGGCGATCTGGACTCGGGTCCGCTGAAGTCGGTCGCCGACAAGCTAATGAGCGCATTGACCGGGCTCGGGC
TGGTGGCGTTTGGCGTCGAGGGAGAAGACTTCGACCCGGTGCTGCACGAAGCCGTGCAGCATGAGGGCGATGGTGGGGAA
GGCTCCAAGCCGGTGATCGGTGACGTCCTCCGGCACGGCTACAAATTGGGCGATCAAGTGCTGCGGCACGCCTTGGTCGG
GGTGGTTGACACGATCGCAGGCGATGGTGCCGAAACGGTTGCCATAGTCGCACCTGTCGACAGCACAGCCAAAACCGAGC
AAGGTGAACTGGGCGATAACGTGACACCGCACAAGGAGGACGGTGATTAG

Upstream 100 bases:

>100_bases
CTGCGTCGAAGGTGGGCGGCGAGGCTTCCGCGCCTGGTGGCTCGAACTCTACTGACGATGTGGTTGACGCGGAGGTGGTC
GACGACGAACGGGAGTCCAA

Downstream 100 bases:

>100_bases
GGGGTGACGCGATATGGCCCAGCGTGAATGGGTCGAAAAGGACTTCTACAAGGAGCTAGGCGTTTCCTCTGACGCCAGTC
CCGAAGAGATTAAGCGTGCC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTEGNPHEQVTVTDKRRIDPDTGEVRSIPLGDTPGGSEPAGMTDRSANSTDTMVDKVAELTSDLQRVQADFANYRKRALR
DQQTASDRAKATVISQLLGVLDDFDRAREHGDLDSGPLKSVADKLMSALTGLGLVAFGVEGEDFDPVLHEAVQHEGDGGE
GSKPVIGDVLRHGYKLGDQVLRHALVGVVDTIAGDGAETVAIVAPVDSTAKTEQGELGDNVTPHKEDGD

Sequences:

>Translated_229_residues
MTEGNPHEQVTVTDKRRIDPDTGEVRSIPLGDTPGGSEPAGMTDRSANSTDTMVDKVAELTSDLQRVQADFANYRKRALR
DQQTASDRAKATVISQLLGVLDDFDRAREHGDLDSGPLKSVADKLMSALTGLGLVAFGVEGEDFDPVLHEAVQHEGDGGE
GSKPVIGDVLRHGYKLGDQVLRHALVGVVDTIAGDGAETVAIVAPVDSTAKTEQGELGDNVTPHKEDGD
>Mature_228_residues
TEGNPHEQVTVTDKRRIDPDTGEVRSIPLGDTPGGSEPAGMTDRSANSTDTMVDKVAELTSDLQRVQADFANYRKRALRD
QQTASDRAKATVISQLLGVLDDFDRAREHGDLDSGPLKSVADKLMSALTGLGLVAFGVEGEDFDPVLHEAVQHEGDGGEG
SKPVIGDVLRHGYKLGDQVLRHALVGVVDTIAGDGAETVAIVAPVDSTAKTEQGELGDNVTPHKEDGD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_MYCLE (Q9CB23)

Other databases:

- EMBL:   AL583925
- PIR:   D87221
- RefSeq:   NP_302612.1
- ProteinModelPortal:   Q9CB23
- SMR:   Q9CB23
- EnsemblBacteria:   EBMYCT00000029071
- GeneID:   908465
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2495
- NMPDR:   fig|272631.1.peg.1484
- Leproma:   ML2495
- GeneTree:   EBGT00050000017239
- HOGENOM:   HBG732630
- OMA:   IGRARDH
- ProtClustDB:   PRK14142
- BioCyc:   MLEP272631:ML2495-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24248; Mature: 24117

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGNPHEQVTVTDKRRIDPDTGEVRSIPLGDTPGGSEPAGMTDRSANSTDTMVDKVAEL
CCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TSDLQRVQADFANYRKRALRDQQTASDRAKATVISQLLGVLDDFDRAREHGDLDSGPLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VADKLMSALTGLGLVAFGVEGEDFDPVLHEAVQHEGDGGEGSKPVIGDVLRHGYKLGDQV
HHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
LRHALVGVVDTIAGDGAETVAIVAPVDSTAKTEQGELGDNVTPHKEDGD
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEGNPHEQVTVTDKRRIDPDTGEVRSIPLGDTPGGSEPAGMTDRSANSTDTMVDKVAEL
CCCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TSDLQRVQADFANYRKRALRDQQTASDRAKATVISQLLGVLDDFDRAREHGDLDSGPLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VADKLMSALTGLGLVAFGVEGEDFDPVLHEAVQHEGDGGEGSKPVIGDVLRHGYKLGDQV
HHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
LRHALVGVVDTIAGDGAETVAIVAPVDSTAKTEQGELGDNVTPHKEDGD
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002