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The map label for this gene is fadB2 [H]

Identifier: 221230809

GI number: 221230809

Start: 2933984

End: 2934847

Strand: Reverse

Name: fadB2 [H]

Synonym: MLBr_02461

Alternate gene names: 221230809

Gene position: 2934847-2933984 (Counterclockwise)

Preceding gene: 221230810

Following gene: 221230806

Centisome position: 89.8

GC content: 57.64

Gene sequence:

>864_bases
GTGAGTGATGCAGCGATCCAGCGAGTAGGGGTTGTTGGGGCGGGGCAGATGGGGTCGGGCATCGCTGAAGTTGCTGTTCG
TGCCGGTGTTGGCGTGACGGTGTTTGAAACCACTGACACGTTAGTCACCGCAGGACGCAACCGCATTGTGAAGTCCTTGG
AGCGTGGCGTTAGCGCAGGCAAGGTGACAGAGCGCGAGCGCGACAGTGCCCTTGGCCATCTGGTCTTCACCACAGACCTG
AATGACTTGGCCGACCGGCAATTGGTGATTGAGGCCATTGTCGAGGACGAGGCTGTCAAGACGAAGGTTTTCGCCGAACT
CGACCGAGTCATTACCGATCCCGATGCGGTGCTTGCGTCGAACACCTCCAGCATCCCGATCATGAAAATCGCTGCGGCCA
CCAAGAACCCACAACGAGTCCTGGGCCTGCACTTCTTCAACCCAGTACCAGTGCTACCCTTGGTCGAATTGGTCAGCACG
TTGGTCACCGATGAAGCCGCTGCCACTCGTGCCGAAGAGTTTGCCAGCGGAGTACTTGGCAAGCAAGTGGTGCGCTGCTC
GGACCGGTCTGGTTTTGTGGTCAATGCTCTACTGGTGCCTTATTTGTTGTCGGCGATCCGGATAGTCGAAGCTGGGTTTG
CCACCGTCGAAGATGTCGACAAGGCTGTCCTTGCCGGGCTGTCGCACCCGATGGGCCCGCTGCGGCTCTCCGATCTCGTT
GGCTTGGACACCCTAAAACTAATCGCGGACAAGATGTTCGAAGAATTTAAAGAACCGCACTACGGTCCGCCGCCGCTGTT
GCTACGTATGGTCGAGGCGGGCCTGCTAGGTAAAAAATCGGGTCAGGGTTTCTATACGTACTGA

Upstream 100 bases:

>100_bases
TTCGAAGGCCTGAGATGAGTTTGACAATTTAGGCCCTGCCCAGCAAACCTAGGTGGGGCCTCTTGCGGTAGAACGACAAT
ACGACTCTGGAGAGACAACA

Downstream 100 bases:

>100_bases
AGTAGCATATGGTCCACCCGCGCGGTTCTTGCCAAGGTCGTTAGCGCCGAGAGGATGTCGAGATCATCCGCAAGCGCGCC
GAGTGGTACAACAGTTGTTG

Product: 3-hydroxybutyryl-CoA dehydrogenase

Products: NA

Alternate protein names: Beta-hydroxybutyryl-CoA dehydrogenase; BHBD [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSDAAIQRVGVVGAGQMGSGIAEVAVRAGVGVTVFETTDTLVTAGRNRIVKSLERGVSAGKVTERERDSALGHLVFTTDL
NDLADRQLVIEAIVEDEAVKTKVFAELDRVITDPDAVLASNTSSIPIMKIAAATKNPQRVLGLHFFNPVPVLPLVELVST
LVTDEAAATRAEEFASGVLGKQVVRCSDRSGFVVNALLVPYLLSAIRIVEAGFATVEDVDKAVLAGLSHPMGPLRLSDLV
GLDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGLLGKKSGQGFYTY

Sequences:

>Translated_287_residues
MSDAAIQRVGVVGAGQMGSGIAEVAVRAGVGVTVFETTDTLVTAGRNRIVKSLERGVSAGKVTERERDSALGHLVFTTDL
NDLADRQLVIEAIVEDEAVKTKVFAELDRVITDPDAVLASNTSSIPIMKIAAATKNPQRVLGLHFFNPVPVLPLVELVST
LVTDEAAATRAEEFASGVLGKQVVRCSDRSGFVVNALLVPYLLSAIRIVEAGFATVEDVDKAVLAGLSHPMGPLRLSDLV
GLDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGLLGKKSGQGFYTY
>Mature_286_residues
SDAAIQRVGVVGAGQMGSGIAEVAVRAGVGVTVFETTDTLVTAGRNRIVKSLERGVSAGKVTERERDSALGHLVFTTDLN
DLADRQLVIEAIVEDEAVKTKVFAELDRVITDPDAVLASNTSSIPIMKIAAATKNPQRVLGLHFFNPVPVLPLVELVSTL
VTDEAAATRAEEFASGVLGKQVVRCSDRSGFVVNALLVPYLLSAIRIVEAGFATVEDVDKAVLAGLSHPMGPLRLSDLVG
LDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGLLGKKSGQGFYTY

Specific function: Catalyzes the NAD-dependent dehydration of beta- hydroxybutyryl-CoA to acetoacetyl-CoA in vitro at pH 10. Also catalyzes the reverse reaction albeit in a lower pH range of 5.5- 6.5. The reverse reaction is able to use NADPH as well as NADH [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI296179429, Length=290, Percent_Identity=41.3793103448276, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI296179427, Length=307, Percent_Identity=39.0879478827362, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI20127408, Length=288, Percent_Identity=36.1111111111111, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI261878539, Length=296, Percent_Identity=31.0810810810811, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI68989263, Length=296, Percent_Identity=31.0810810810811, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI115430219, Length=260, Percent_Identity=26.1538461538462, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787661, Length=285, Percent_Identity=42.1052631578947, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1788682, Length=268, Percent_Identity=33.955223880597, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790281, Length=267, Percent_Identity=30.3370786516854, Blast_Score=120, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17549919, Length=291, Percent_Identity=39.8625429553265, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17553560, Length=289, Percent_Identity=36.3321799307958, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17563036, Length=291, Percent_Identity=38.4879725085911, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17508951, Length=288, Percent_Identity=32.9861111111111, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17508953, Length=288, Percent_Identity=32.9861111111111, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25144276, Length=288, Percent_Identity=32.9861111111111, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17558304, Length=283, Percent_Identity=32.1554770318021, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71985923, Length=284, Percent_Identity=27.8169014084507, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71985930, Length=288, Percent_Identity=27.4305555555556, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI115533614, Length=175, Percent_Identity=34.2857142857143, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193203623, Length=175, Percent_Identity=34.2857142857143, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921000, Length=284, Percent_Identity=35.5633802816901, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24583077, Length=284, Percent_Identity=35.5633802816901, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24583079, Length=284, Percent_Identity=35.5633802816901, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45549573, Length=276, Percent_Identity=28.2608695652174, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129993, Length=248, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022694
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N [H]

EC number: =1.1.1.157 [H]

Molecular weight: Translated: 30566; Mature: 30434

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAIQRVGVVGAGQMGSGIAEVAVRAGVGVTVFETTDTLVTAGRNRIVKSLERGVSAG
CCCHHHHHHCCEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
KVTERERDSALGHLVFTTDLNDLADRQLVIEAIVEDEAVKTKVFAELDRVITDPDAVLAS
CCHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHC
NTSSIPIMKIAAATKNPQRVLGLHFFNPVPVLPLVELVSTLVTDEAAATRAEEFASGVLG
CCCCCCEEEEEECCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVVRCSDRSGFVVNALLVPYLLSAIRIVEAGFATVEDVDKAVLAGLSHPMGPLRLSDLV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
GLDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGLLGKKSGQGFYTY
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SDAAIQRVGVVGAGQMGSGIAEVAVRAGVGVTVFETTDTLVTAGRNRIVKSLERGVSAG
CCHHHHHHCCEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
KVTERERDSALGHLVFTTDLNDLADRQLVIEAIVEDEAVKTKVFAELDRVITDPDAVLAS
CCHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHC
NTSSIPIMKIAAATKNPQRVLGLHFFNPVPVLPLVELVSTLVTDEAAATRAEEFASGVLG
CCCCCCEEEEEECCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVVRCSDRSGFVVNALLVPYLLSAIRIVEAGFATVEDVDKAVLAGLSHPMGPLRLSDLV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
GLDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGLLGKKSGQGFYTY
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]