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The map label for this gene is hemA

Identifier: 221230781

GI number: 221230781

Start: 2897392

End: 2898795

Strand: Reverse

Name: hemA

Synonym: MLBr_02422

Alternate gene names: 221230781

Gene position: 2898795-2897392 (Counterclockwise)

Preceding gene: 221230785

Following gene: 221230780

Centisome position: 88.7

GC content: 62.96

Gene sequence:

>1404_bases
GTGAGCATCTTGCTCTTCGGGGTTTCGCACCGCAGTGCGCCGGTCTCTGTCTTAGAACAACTCAGCCTGGACAGATCCGA
TCAGATCAAGATCGTCGACCGGGTGCTGCAGTCCCCGCTGGTGACCGAGGCGATGGTGCTGTCGACCTGTAACCGTGTCG
AGGTTTACGCCGTGGTGGAAGCGTTCCACGCGGGATTATCAGTGATTGGGCAAGTGCTGTCGGAATACTCCGCGATGTCG
ATCGGGGACCTGACCAAATACGCTTATGTACGCTACAGCGAGGCTGCTGTCGAGCACTTGTTCACGGTTGCCAGCGGTCT
GGATTCAGCGGTGGTCGGCGAGCAGCAGGTGCTGGGTCAGGTGCGTCGCGCGTATGCCGCCGCTGAGGCTAATCGCACGG
TAGGCCAGGTGTTGCATGAGGTGGCCCAACGAGCGTTGTCGGTGGGTAAGCGGGTGCATTCCGAAACTGCCATTGACGCT
GCCGGGGTCTCGGTGGTGTCGGTCGCCCTTGGCATAGCTGGACGCACATTGGGCGGGCTGGCGGGCCGGATCGCGGTGGT
GATTGGCGCTGGCGCGATGGGTGCACTCTCGTCGTCGTACCTGACGCAGGCCAACGTCGGACGTATACATGTACTCAACC
GGTCGTTGTCTCGAGCGCGGCGGCTGGCCGGCAAGATCGGTGAATCGGGCGTGCCGGCCGACGTGTGGACTCTTAATCAC
CTTGACGAAGCATTGGCGGACGCTGACCTAGTGGTCAGCTGTACGGGGGCGGTAAGCCCCGTGGTTTCGCTGGCTGACGT
GCATCATGCATTGGCCGCTATGCGCCGTGACGAAACGACTCGGCCGCTGGTGATATGCGATTTGGGCATGCCGCGTGACG
TGGATCCGGCTGTGGCCAAACTGCCCGGCGTTTGGGTCGTCGATGTGGAAGGCGTGCAGCGTGAACCGTCGGCCCGTGCC
AGTGCCGCCGACGTCGATGCCGCGCGCCGCATTGTGGCGGCTGAAGTGGCCACATATCTGACTAGGCAGCGGATGGCCGA
GGTGGCCCCGACGGTGACAGCGTTGCGTCAGCGAGCTGCCGATGTGGTCGAAGCGGAGTTACTGCGCCTAGACCACCGGC
TGCCAGGGCTGGAAAGCGCTCAGCGCGAGGAGGTGGCACGCACCGTGCGACGGGTGGTGGACAAGCTGCTACACGCGCCT
ACCGTGCGGATCAAGCAGCTTGCCAGTGCTCCCGGCGGTGACAGCTACACCGAGGCGCTGCGTGAGCTTTTCGAGCTTGA
TCAGACCGCTGTCGACGCGGTCGCCACCGCGGGTGAATTACCAGTGATGGTAAGTGGGTTCAGCGATACCACTAGGTACG
GCACAAGCCCTGCCCAGAGCAGCTCAAAATATCACGCCGAGTAG

Upstream 100 bases:

>100_bases
ATCCGCTGACAATATTTGGTAGCCCACTTGATCAACGTCTACCGTAGACACCAGCCTGACTACTGGAGGAAAGCAAGGGA
GCTGGCCAAGTGATGCTGCC

Downstream 100 bases:

>100_bases
TTGATTGGCCCACGTGATCCGGATAGGCACTCGGGGTAGCTTGTTAGCCACCACTCAGGCTGCGCTCGTTAGAGACGCTC
TCATAGCCAACGGGCATCCT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSILLFGVSHRSAPVSVLEQLSLDRSDQIKIVDRVLQSPLVTEAMVLSTCNRVEVYAVVEAFHAGLSVIGQVLSEYSAMS
IGDLTKYAYVRYSEAAVEHLFTVASGLDSAVVGEQQVLGQVRRAYAAAEANRTVGQVLHEVAQRALSVGKRVHSETAIDA
AGVSVVSVALGIAGRTLGGLAGRIAVVIGAGAMGALSSSYLTQANVGRIHVLNRSLSRARRLAGKIGESGVPADVWTLNH
LDEALADADLVVSCTGAVSPVVSLADVHHALAAMRRDETTRPLVICDLGMPRDVDPAVAKLPGVWVVDVEGVQREPSARA
SAADVDAARRIVAAEVATYLTRQRMAEVAPTVTALRQRAADVVEAELLRLDHRLPGLESAQREEVARTVRRVVDKLLHAP
TVRIKQLASAPGGDSYTEALRELFELDQTAVDAVATAGELPVMVSGFSDTTRYGTSPAQSSSKYHAE

Sequences:

>Translated_467_residues
MSILLFGVSHRSAPVSVLEQLSLDRSDQIKIVDRVLQSPLVTEAMVLSTCNRVEVYAVVEAFHAGLSVIGQVLSEYSAMS
IGDLTKYAYVRYSEAAVEHLFTVASGLDSAVVGEQQVLGQVRRAYAAAEANRTVGQVLHEVAQRALSVGKRVHSETAIDA
AGVSVVSVALGIAGRTLGGLAGRIAVVIGAGAMGALSSSYLTQANVGRIHVLNRSLSRARRLAGKIGESGVPADVWTLNH
LDEALADADLVVSCTGAVSPVVSLADVHHALAAMRRDETTRPLVICDLGMPRDVDPAVAKLPGVWVVDVEGVQREPSARA
SAADVDAARRIVAAEVATYLTRQRMAEVAPTVTALRQRAADVVEAELLRLDHRLPGLESAQREEVARTVRRVVDKLLHAP
TVRIKQLASAPGGDSYTEALRELFELDQTAVDAVATAGELPVMVSGFSDTTRYGTSPAQSSSKYHAE
>Mature_466_residues
SILLFGVSHRSAPVSVLEQLSLDRSDQIKIVDRVLQSPLVTEAMVLSTCNRVEVYAVVEAFHAGLSVIGQVLSEYSAMSI
GDLTKYAYVRYSEAAVEHLFTVASGLDSAVVGEQQVLGQVRRAYAAAEANRTVGQVLHEVAQRALSVGKRVHSETAIDAA
GVSVVSVALGIAGRTLGGLAGRIAVVIGAGAMGALSSSYLTQANVGRIHVLNRSLSRARRLAGKIGESGVPADVWTLNHL
DEALADADLVVSCTGAVSPVVSLADVHHALAAMRRDETTRPLVICDLGMPRDVDPAVAKLPGVWVVDVEGVQREPSARAS
AADVDAARRIVAAEVATYLTRQRMAEVAPTVTALRQRAADVVEAELLRLDHRLPGLESAQREEVARTVRRVVDKLLHAPT
VRIKQLASAPGGDSYTEALRELFELDQTAVDAVATAGELPVMVSGFSDTTRYGTSPAQSSSKYHAE

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=434, Percent_Identity=32.4884792626728, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_MYCLE (P46724)

Other databases:

- EMBL:   U00018
- EMBL:   AL583925
- PIR:   S72907
- RefSeq:   NP_302567.1
- ProteinModelPortal:   P46724
- EnsemblBacteria:   EBMYCT00000029023
- GeneID:   909785
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2422
- NMPDR:   fig|272631.1.peg.1439
- Leproma:   ML2422
- GeneTree:   EBGT00050000017163
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   MLEP272631:ML2422-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 49484; Mature: 49353

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00747 GLUTR; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SILLFGVSHRSAPVSVLEQLSLDRSDQIKIVDRVLQSPLVTEAMVLSTCNRVEVYAVVE
EEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHH
AFHAGLSVIGQVLSEYSAMSIGDLTKYAYVRYSEAAVEHLFTVASGLDSAVVGEQQVLGQ
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHH
VRRAYAAAEANRTVGQVLHEVAQRALSVGKRVHSETAIDAAGVSVVSVALGIAGRTLGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRIAVVIGAGAMGALSSSYLTQANVGRIHVLNRSLSRARRLAGKIGESGVPADVWTLNH
HHHHHHEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LDEALADADLVVSCTGAVSPVVSLADVHHALAAMRRDETTRPLVICDLGMPRDVDPAVAK
HHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHH
LPGVWVVDVEGVQREPSARASAADVDAARRIVAAEVATYLTRQRMAEVAPTVTALRQRAA
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVVEAELLRLDHRLPGLESAQREEVARTVRRVVDKLLHAPTVRIKQLASAPGGDSYTEAL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHH
RELFELDQTAVDAVATAGELPVMVSGFSDTTRYGTSPAQSSSKYHAE
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002