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The map label for this gene is metB

Identifier: 221230764

GI number: 221230764

Start: 2863689

End: 2864855

Strand: Reverse

Name: metB

Synonym: MLBr_02394

Alternate gene names: 221230764

Gene position: 2864855-2863689 (Counterclockwise)

Preceding gene: 221230765

Following gene: 221230761

Centisome position: 87.66

GC content: 60.07

Gene sequence:

>1167_bases
ATGAGCGAAGATTACCGGGGACACCACGGCATTACCGGACTAGCCACCAAAGCCATCCATGCTGGCTATCGTCCGGATCC
GGCAACAGGGGCAGTGAATGTCCCGATTTATGCCAGTAGTACTTTTGCCCAAGATGGCGTCGGTGAGTTGCGTGGCGGAT
TCGAATACGCGCGTACCGGCAACCCCATGCGCGCCGCTTTAGAGGCATCCTTGGCCACGGTCGAAGAGGGCGTTTTTGCG
CGAGCCTTCAGTTCCGGAATGGCTGCTAGCGACTGTGCCTTGCGGGTCATGCTGCGGCCGGGGGACCACGTGATCATCCC
GGATGACGTCTACGGCGGCACCTTCCGGCTGATAGACAAGGTCTTTACTCAATGGAACGTTGACTACACGCCGGTACCGC
TGTCTGATTTGGACGCGGTCCGCGCCGCGATCACATCACGGACCCGGCTGATATGGGTGGAAACACCGACCAATCCGCTG
CTGTCCATCGCAGATATCACCAGCATCGGCGAACTAGGCAAAAAGCACTCAGTAAAGGTGTTGGTGGACAACACCTTTGC
TTCACCCGCGCTGCAACAGCCGCTGATGCTGGGGGCAGACGTCGTGTTGCACTCGACCACAAAGTACATCGGCGGCCACT
CTGATGTGGTGGGCGGCGCGCTAGTCACCAACGACGAAGAGCTGGACCAGGCTTTCGGCTTCTTGCAGAACGGAGCCGGT
GCGGTGCCGAGCCCGTTCGACGCGTACCTAACGATGCGCGGATTGAAGACTTTAGTGCTGCGGATGCAGCGGCACAACGA
AAATGCCATTACTGTAGCGGAATTCCTGGCTGGGCATCCGTCGGTGAGCGCCGTGCTGTATCCGGGCTTGCCCAGCCATC
CCGGGCATGAGGTCGCTGCACGGCAGATGCGCGGCTTCGGCGGCATGGTTTCGTTGCGGATGCGAGCCGGCCGACTAGCC
GCCCAGGATCTGTGTGCCCGCACCAAGGTGTTTACCTTGGCTGAATCCTTGGGTGGAGTGGAGTCGCTGATTGAGCAGCC
CAGTGCCATGACGCACGCGTCGACAACCGGGTCGCAATTGGAAGTACCCGACGACCTGGTGCGGCTTTCGGTCGGTATTG
AAGACGTCGGCGACCTGCTGTGCGACCTCAAGCAGGCGTTAAACTAA

Upstream 100 bases:

>100_bases
ACGGTGTGTCTGCCGATCTGACACAGTGCCACGACTTGGCAGGCTAGTCGGCGGCCCCCCAACCGCTGACCGCAGCGGCC
ATCTCTTAGGCTAATCGTCG

Downstream 100 bases:

>100_bases
CTGCGCCGACTGTGAATCTGATGACGCGACGCCGGCCGTCGCGGGATTCACACTTGCGATAATTATGAGTGGTAAGGCTC
AGCGCTGATCAACGTGACTT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSEDYRGHHGITGLATKAIHAGYRPDPATGAVNVPIYASSTFAQDGVGELRGGFEYARTGNPMRAALEASLATVEEGVFA
RAFSSGMAASDCALRVMLRPGDHVIIPDDVYGGTFRLIDKVFTQWNVDYTPVPLSDLDAVRAAITSRTRLIWVETPTNPL
LSIADITSIGELGKKHSVKVLVDNTFASPALQQPLMLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDQAFGFLQNGAG
AVPSPFDAYLTMRGLKTLVLRMQRHNENAITVAEFLAGHPSVSAVLYPGLPSHPGHEVAARQMRGFGGMVSLRMRAGRLA
AQDLCARTKVFTLAESLGGVESLIEQPSAMTHASTTGSQLEVPDDLVRLSVGIEDVGDLLCDLKQALN

Sequences:

>Translated_388_residues
MSEDYRGHHGITGLATKAIHAGYRPDPATGAVNVPIYASSTFAQDGVGELRGGFEYARTGNPMRAALEASLATVEEGVFA
RAFSSGMAASDCALRVMLRPGDHVIIPDDVYGGTFRLIDKVFTQWNVDYTPVPLSDLDAVRAAITSRTRLIWVETPTNPL
LSIADITSIGELGKKHSVKVLVDNTFASPALQQPLMLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDQAFGFLQNGAG
AVPSPFDAYLTMRGLKTLVLRMQRHNENAITVAEFLAGHPSVSAVLYPGLPSHPGHEVAARQMRGFGGMVSLRMRAGRLA
AQDLCARTKVFTLAESLGGVESLIEQPSAMTHASTTGSQLEVPDDLVRLSVGIEDVGDLLCDLKQALN
>Mature_387_residues
SEDYRGHHGITGLATKAIHAGYRPDPATGAVNVPIYASSTFAQDGVGELRGGFEYARTGNPMRAALEASLATVEEGVFAR
AFSSGMAASDCALRVMLRPGDHVIIPDDVYGGTFRLIDKVFTQWNVDYTPVPLSDLDAVRAAITSRTRLIWVETPTNPLL
SIADITSIGELGKKHSVKVLVDNTFASPALQQPLMLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDQAFGFLQNGAGA
VPSPFDAYLTMRGLKTLVLRMQRHNENAITVAEFLAGHPSVSAVLYPGLPSHPGHEVAARQMRGFGGMVSLRMRAGRLAA
QDLCARTKVFTLAESLGGVESLIEQPSAMTHASTTGSQLEVPDDLVRLSVGIEDVGDLLCDLKQALN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=46.7032967032967, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI299473759, Length=364, Percent_Identity=42.8571428571429, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI25453487, Length=363, Percent_Identity=41.3223140495868, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1790375, Length=376, Percent_Identity=40.9574468085106, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=30.4347826086957, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=382, Percent_Identity=45.5497382198953, Blast_Score=331, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17538055, Length=382, Percent_Identity=47.1204188481675, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI71980708, Length=376, Percent_Identity=38.2978723404255, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=48.4455958549223, Blast_Score=323, Evalue=4e-89,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=33.0882352941176, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=29.8368298368298, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=334, Percent_Identity=29.6407185628743, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=30.2030456852792, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=48.1675392670157, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METB_MYCLE (P46807)

Other databases:

- EMBL:   U15183
- EMBL:   AL583925
- PIR:   F87208
- RefSeq:   NP_302550.1
- ProteinModelPortal:   P46807
- SMR:   P46807
- EnsemblBacteria:   EBMYCT00000028489
- GeneID:   908547
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2394
- NMPDR:   fig|272631.1.peg.1422
- Leproma:   ML2394
- GeneTree:   EBGT00050000016651
- HOGENOM:   HBG754729
- OMA:   RTEIFIL
- ProtClustDB:   PRK07811
- BioCyc:   MLEP272631:ML2394-MONOMER
- BRENDA:   2.5.1.48
- GO:   GO:0005737
- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11808
- PIRSF:   PIRSF001434

Pfam domain/function: PF01053 Cys_Met_Meta_PP; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.5.1.48

Molecular weight: Translated: 41292; Mature: 41161

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDYRGHHGITGLATKAIHAGYRPDPATGAVNVPIYASSTFAQDGVGELRGGFEYARTG
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCHHHHCC
NPMRAALEASLATVEEGVFARAFSSGMAASDCALRVMLRPGDHVIIPDDVYGGTFRLIDK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHH
VFTQWNVDYTPVPLSDLDAVRAAITSRTRLIWVETPTNPLLSIADITSIGELGKKHSVKV
HHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHCCCCCCEEE
LVDNTFASPALQQPLMLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDQAFGFLQNGAG
EEECCCCCHHHHCCHHHCCHHHEECHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCC
AVPSPFDAYLTMRGLKTLVLRMQRHNENAITVAEFLAGHPSVSAVLYPGLPSHPGHEVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCHHHHH
RQMRGFGGMVSLRMRAGRLAAQDLCARTKVFTLAESLGGVESLIEQPSAMTHASTTGSQL
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHCCCCCCCCC
EVPDDLVRLSVGIEDVGDLLCDLKQALN
CCCHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEDYRGHHGITGLATKAIHAGYRPDPATGAVNVPIYASSTFAQDGVGELRGGFEYARTG
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCHHHHCC
NPMRAALEASLATVEEGVFARAFSSGMAASDCALRVMLRPGDHVIIPDDVYGGTFRLIDK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHH
VFTQWNVDYTPVPLSDLDAVRAAITSRTRLIWVETPTNPLLSIADITSIGELGKKHSVKV
HHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHCCCCCCEEE
LVDNTFASPALQQPLMLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDQAFGFLQNGAG
EEECCCCCHHHHCCHHHCCHHHEECHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCC
AVPSPFDAYLTMRGLKTLVLRMQRHNENAITVAEFLAGHPSVSAVLYPGLPSHPGHEVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCHHHHH
RQMRGFGGMVSLRMRAGRLAAQDLCARTKVFTLAESLGGVESLIEQPSAMTHASTTGSQL
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHCCCCCCCCC
EVPDDLVRLSVGIEDVGDLLCDLKQALN
CCCHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002