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The map label for this gene is yciL [C]

Identifier: 221230192

GI number: 221230192

Start: 1630368

End: 1631141

Strand: Direct

Name: yciL [C]

Synonym: MLBr_01370

Alternate gene names: 221230192

Gene position: 1630368-1631141 (Clockwise)

Preceding gene: 221230191

Following gene: 221230193

Centisome position: 49.89

GC content: 59.69

Gene sequence:

>774_bases
TTGATGGTCGAGATCCACGATTCTAGGATGGACCGGGGTGCTGGGGCGGTCCGTCTGCAAAAGATATTGTCCCGGGCTGG
GATTGCGTCGCGGCGGGCCGCCGAGAAGCTAATCATCGAAGGCCGTGTCGAAGTGGACGGTCAGCTGGTGAGGGAGTTGG
GTACCCGGGTCGACCCGGATGTCTCGGTAGTTCGGGTCGATGGGGTCAAGGTAGTGGTCGACGACTCGCTGGTGTATCTG
GCGCTGAACAAGCCGCGCGGCATGTACTCGACCATGTCGGACGACCGCGGCCGGCCCTGTGTCGGTGATTTGATCGAGCG
CCGGGTGCGGGGCAATAAGAAGCTGTTTCATGTAGGGCGGCTAGACGCCGATACCGAAGGGCTGATCTTGTTGACTAACG
ATGGCGAGTTAGCACACCGGTTGATGCATCCGTCCCATGAGGTGTCCAAGACGTATTTGGCGACGGTGAAAGGGGCGGTT
CCGCGTGGGTTAGGTAAGAAGCTAAGCGTGGGACTTGAGTTGGACGACGGCCCGGCGCATGTCGATGATTTCGCGGTGGT
AGACGCGATCCCAGGTAAGACGTTGGTGCGGTTGACGTTGCACGAAGGACGTAAGCGCATTGTACGTCGGCTACTGACAG
CTGCAGGCTTCCCTGTAGAGATGCTGGTCCGCACCGATATCGGTGCGGTGTCGCTGGGAGATCAGCGTCCGGGCTGCCTT
CGGGCTTTGCTCCGTGATGAGATCAGACAACTGTATAAAGAGGTGGGCCTGTGA

Upstream 100 bases:

>100_bases
TCGAAGACATGAGCGAGTTTCTGGACAAAAAGCCACGTTTCATCAAGCTTACCGGCGCCAAAGTGTCTAAGCAGCCACGG
TCGTTCGATGTGAACCAGGA

Downstream 100 bases:

>100_bases
CCGATATTGTTGTCGCCATCGACGGACCGGCGGGGACCGGAAAATCTTCCGTGTCAAGGGGACTAGCGCGTGAGCTGGGG
GCGCGCTACTTAGATACCGG

Product: putative pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MMVEIHDSRMDRGAGAVRLQKILSRAGIASRRAAEKLIIEGRVEVDGQLVRELGTRVDPDVSVVRVDGVKVVVDDSLVYL
ALNKPRGMYSTMSDDRGRPCVGDLIERRVRGNKKLFHVGRLDADTEGLILLTNDGELAHRLMHPSHEVSKTYLATVKGAV
PRGLGKKLSVGLELDDGPAHVDDFAVVDAIPGKTLVRLTLHEGRKRIVRRLLTAAGFPVEMLVRTDIGAVSLGDQRPGCL
RALLRDEIRQLYKEVGL

Sequences:

>Translated_257_residues
MMVEIHDSRMDRGAGAVRLQKILSRAGIASRRAAEKLIIEGRVEVDGQLVRELGTRVDPDVSVVRVDGVKVVVDDSLVYL
ALNKPRGMYSTMSDDRGRPCVGDLIERRVRGNKKLFHVGRLDADTEGLILLTNDGELAHRLMHPSHEVSKTYLATVKGAV
PRGLGKKLSVGLELDDGPAHVDDFAVVDAIPGKTLVRLTLHEGRKRIVRRLLTAAGFPVEMLVRTDIGAVSLGDQRPGCL
RALLRDEIRQLYKEVGL
>Mature_257_residues
MMVEIHDSRMDRGAGAVRLQKILSRAGIASRRAAEKLIIEGRVEVDGQLVRELGTRVDPDVSVVRVDGVKVVVDDSLVYL
ALNKPRGMYSTMSDDRGRPCVGDLIERRVRGNKKLFHVGRLDADTEGLILLTNDGELAHRLMHPSHEVSKTYLATVKGAV
PRGLGKKLSVGLELDDGPAHVDDFAVVDAIPGKTLVRLTLHEGRKRIVRRLLTAAGFPVEMLVRTDIGAVSLGDQRPGCL
RALLRDEIRQLYKEVGL

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=220, Percent_Identity=42.2727272727273, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=36.0515021459227, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=34.1666666666667, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=32.9545454545455, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28245; Mature: 28245

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVEIHDSRMDRGAGAVRLQKILSRAGIASRRAAEKLIIEGRVEVDGQLVRELGTRVDPD
CEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCC
VSVVRVDGVKVVVDDSLVYLALNKPRGMYSTMSDDRGRPCVGDLIERRVRGNKKLFHVGR
CEEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
LDADTEGLILLTNDGELAHRLMHPSHEVSKTYLATVKGAVPRGLGKKLSVGLELDDGPAH
CCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
VDDFAVVDAIPGKTLVRLTLHEGRKRIVRRLLTAAGFPVEMLVRTDIGAVSLGDQRPGCL
CCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCHHH
RALLRDEIRQLYKEVGL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMVEIHDSRMDRGAGAVRLQKILSRAGIASRRAAEKLIIEGRVEVDGQLVRELGTRVDPD
CEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHHHHHCCCCCCC
VSVVRVDGVKVVVDDSLVYLALNKPRGMYSTMSDDRGRPCVGDLIERRVRGNKKLFHVGR
CEEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
LDADTEGLILLTNDGELAHRLMHPSHEVSKTYLATVKGAVPRGLGKKLSVGLELDDGPAH
CCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
VDDFAVVDAIPGKTLVRLTLHEGRKRIVRRLLTAAGFPVEMLVRTDIGAVSLGDQRPGCL
CCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCHHH
RALLRDEIRQLYKEVGL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]