Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 221230187

GI number: 221230187

Start: 1624085

End: 1625857

Strand: Direct

Name: pyrG

Synonym: MLBr_01363

Alternate gene names: 221230187

Gene position: 1624085-1625857 (Clockwise)

Preceding gene: 221230186

Following gene: 221230188

Centisome position: 49.7

GC content: 60.01

Gene sequence:

>1773_bases
GTGCGTAAGCACCCGCAATCCGCTACCAAGCACCTCTTTGTCAGCGGTGGCGTCGCTTCTTCGCTTGGCAAAGGACTCAC
CGCGAGCAGCCTCGGGCAACTGTTGACCGCTCGTGGGTTGCACGTGACAATGCAGAAACTCGACCCGTACCTCAATGTTG
ACCCGGGCACCATGAACCCGTTCCAGCACGGGGAGGTCTTTGTGACCGAGGATGGCGCCGAGACTGACCTCGACGTCGGC
CACTACGAGCGGTTCCTGGATCGCGATCTGTCCGGCTCGGCGAATGTCACTACCGGGCAGGTGTATTCGACAGTGATCGC
CAAAGAACGGCGTGGCGAATATCTCGGTGACACAGTCCAGGTGATCCCGCACATCACCGACGAGATCAAGCAGCGCATCA
TGGCGATGGCCCAGCCCGACGGCGGAGACAACCGCCCAGACGTCGTCATTACCGAAATCGGTGGCACCGTGGGTGATATC
GAATCGCAGCCCTTTTTGGAGGCGGCACGGCAGGTCCGTCACGACCTTGGCCGGGAGAACGTCTTCTTTCTACATGTGTC
GTTGGTGCCTCACCTGGCGCCTTCGGGTGAACTCAAAACCAAGCCTACCCAGCACTCAGTGGCCGCGCTGCGCAGCATCG
GTATTACCCCGGATGCCCTGATCCTGCGCTGCGACCGAGATGTTCCCGAATCGTTGAAGAACAAGATTGCGCTGATGTGC
GACGTTGATATCGATGGAGTCATCTCCACCCCGGACGCGCCATCGATCTATGACATACCCAAAGTGTTGCATCGTGAGGA
ACTCGACGCCTTTGTGGTGCGCCGACTCAATCTACCGTTCCGCGACGTGGACTGGACCGAGTGGGACGACTTGCTGCGCC
GTGTTCACGAGCCGCACGGGACTGTGCGAATTGCTCTTGTAGGTAAGTACGTTGACTTTTCCGACGCCTATCTGTCAGTC
TCCGAAGCGCTACATGCCGGCGGGTTCAAACATTACGCCAAGGTTGAGGTAGTTTGGGTGGCTTCCGACGATTGCGAAAC
TGCCACGGGCGCTGCAGCGGTGCTGGCCGACGTTCACGGTGTGCTGATCCCTGGTGGGTTCGGTATCCGCGGAATCGAAG
GAAAAATCGGCGCCATCCGGTATGCTCGGGCTCGGGGGCTGCCGGTGCTTGGCCTATGCTTGGGTTTGCAATGTATCGTG
ATCGAGGCCACCCGCTCGGTCGGTCTCGTGCAAGCGAACTCGGCCGAATTCGAACCAGCTACACCAGATCCGGTGATCTC
AACGATGGCCGATCAAAAAGAGATCGTGGCCGGCGAAGCGGATTTCGGCGGCACGATGCGGCTGGGGGCCTATCCCGCGG
TTTTGCAACCGGCTTCGATCGTGGCCCAAGCGTATGGCACAACGCAGGTGTCCGAGCGACACCGGCACCGCTACGAAGTC
AACAACGCCTACCGCGATTGGATCGCCGAAAGTGGGCTGCGGATTTCTGGAACTTCGCCCGACGGTTATCTGGTGGAGTT
CGTCGAATATCCGGCCAACATGCATCCATTCGTTGTCGGCACCCAGGCTCACCCTGAACTGAAGAGCCGACCCACCCGGC
CGCATCCACTGTTCGTCGCTTTCGTCGGGGCGGCCATCGACTACAAGTCGGCGGAGTTGCTCCCTGTGGAGATACCTGCG
GTCCCGGAAATTTCTGAGCACTTGCCCAACAGCAGTAACCAGCATCGAGATGGCGTCGAGCGGTCGTTCCCGGCACCTGC
AGCCCGTGGCTGA

Upstream 100 bases:

>100_bases
GGGTTTCCGTAAGTTGGCAGATCCAGCTGGCAACGGCCAGAAACATCTTGGCAGAGTCCTGGAAAAATATGCGCTGCCCG
TCTTCACGGAGGTCGTCTTA

Downstream 100 bases:

>100_bases
GCACGTCTTCAAGACGACTTCGTTCGAAACGGCGGATCGCGGTAACATTTCTGCGCTGCGTCGCGACCAGGTACCGATGC
TCGGTGGCCGCACCCATGGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MRKHPQSATKHLFVSGGVASSLGKGLTASSLGQLLTARGLHVTMQKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDVG
HYERFLDRDLSGSANVTTGQVYSTVIAKERRGEYLGDTVQVIPHITDEIKQRIMAMAQPDGGDNRPDVVITEIGGTVGDI
ESQPFLEAARQVRHDLGRENVFFLHVSLVPHLAPSGELKTKPTQHSVAALRSIGITPDALILRCDRDVPESLKNKIALMC
DVDIDGVISTPDAPSIYDIPKVLHREELDAFVVRRLNLPFRDVDWTEWDDLLRRVHEPHGTVRIALVGKYVDFSDAYLSV
SEALHAGGFKHYAKVEVVWVASDDCETATGAAAVLADVHGVLIPGGFGIRGIEGKIGAIRYARARGLPVLGLCLGLQCIV
IEATRSVGLVQANSAEFEPATPDPVISTMADQKEIVAGEADFGGTMRLGAYPAVLQPASIVAQAYGTTQVSERHRHRYEV
NNAYRDWIAESGLRISGTSPDGYLVEFVEYPANMHPFVVGTQAHPELKSRPTRPHPLFVAFVGAAIDYKSAELLPVEIPA
VPEISEHLPNSSNQHRDGVERSFPAPAARG

Sequences:

>Translated_590_residues
MRKHPQSATKHLFVSGGVASSLGKGLTASSLGQLLTARGLHVTMQKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDVG
HYERFLDRDLSGSANVTTGQVYSTVIAKERRGEYLGDTVQVIPHITDEIKQRIMAMAQPDGGDNRPDVVITEIGGTVGDI
ESQPFLEAARQVRHDLGRENVFFLHVSLVPHLAPSGELKTKPTQHSVAALRSIGITPDALILRCDRDVPESLKNKIALMC
DVDIDGVISTPDAPSIYDIPKVLHREELDAFVVRRLNLPFRDVDWTEWDDLLRRVHEPHGTVRIALVGKYVDFSDAYLSV
SEALHAGGFKHYAKVEVVWVASDDCETATGAAAVLADVHGVLIPGGFGIRGIEGKIGAIRYARARGLPVLGLCLGLQCIV
IEATRSVGLVQANSAEFEPATPDPVISTMADQKEIVAGEADFGGTMRLGAYPAVLQPASIVAQAYGTTQVSERHRHRYEV
NNAYRDWIAESGLRISGTSPDGYLVEFVEYPANMHPFVVGTQAHPELKSRPTRPHPLFVAFVGAAIDYKSAELLPVEIPA
VPEISEHLPNSSNQHRDGVERSFPAPAARG
>Mature_590_residues
MRKHPQSATKHLFVSGGVASSLGKGLTASSLGQLLTARGLHVTMQKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDVG
HYERFLDRDLSGSANVTTGQVYSTVIAKERRGEYLGDTVQVIPHITDEIKQRIMAMAQPDGGDNRPDVVITEIGGTVGDI
ESQPFLEAARQVRHDLGRENVFFLHVSLVPHLAPSGELKTKPTQHSVAALRSIGITPDALILRCDRDVPESLKNKIALMC
DVDIDGVISTPDAPSIYDIPKVLHREELDAFVVRRLNLPFRDVDWTEWDDLLRRVHEPHGTVRIALVGKYVDFSDAYLSV
SEALHAGGFKHYAKVEVVWVASDDCETATGAAAVLADVHGVLIPGGFGIRGIEGKIGAIRYARARGLPVLGLCLGLQCIV
IEATRSVGLVQANSAEFEPATPDPVISTMADQKEIVAGEADFGGTMRLGAYPAVLQPASIVAQAYGTTQVSERHRHRYEV
NNAYRDWIAESGLRISGTSPDGYLVEFVEYPANMHPFVVGTQAHPELKSRPTRPHPLFVAFVGAAIDYKSAELLPVEIPA
VPEISEHLPNSSNQHRDGVERSFPAPAARG

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=45.0628366247756, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=561, Percent_Identity=42.4242424242424, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI28559083, Length=561, Percent_Identity=42.4242424242424, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI221316689, Length=561, Percent_Identity=42.4242424242424, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI1789142, Length=552, Percent_Identity=50.1811594202899, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI25148299, Length=648, Percent_Identity=35.9567901234568, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6322563, Length=566, Percent_Identity=42.9328621908127, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319432, Length=566, Percent_Identity=41.8727915194346, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24664469, Length=555, Percent_Identity=44.6846846846847, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21357815, Length=500, Percent_Identity=43.6, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_MYCLB (B8ZRH6)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503603.1
- ProteinModelPortal:   B8ZRH6
- SMR:   B8ZRH6
- EnsemblBacteria:   EBMYCT00000086311
- GeneID:   7326503
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016104
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 64082; Mature: 64082

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 393-393 ACT_SITE 524-524 ACT_SITE 526-526

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKHPQSATKHLFVSGGVASSLGKGLTASSLGQLLTARGLHVTMQKLDPYLNVDPGTMNP
CCCCCCCHHCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCEEEHHHCCCCEECCCCCCCC
FQHGEVFVTEDGAETDLDVGHYERFLDRDLSGSANVTTGQVYSTVIAKERRGEYLGDTVQ
CCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
VIPHITDEIKQRIMAMAQPDGGDNRPDVVITEIGGTVGDIESQPFLEAARQVRHDLGREN
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
VFFLHVSLVPHLAPSGELKTKPTQHSVAALRSIGITPDALILRCDRDVPESLKNKIALMC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCEEEEE
DVDIDGVISTPDAPSIYDIPKVLHREELDAFVVRRLNLPFRDVDWTEWDDLLRRVHEPHG
ECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
TVRIALVGKYVDFSDAYLSVSEALHAGGFKHYAKVEVVWVASDDCETATGAAAVLADVHG
CEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCC
VLIPGGFGIRGIEGKIGAIRYARARGLPVLGLCLGLQCIVIEATRSVGLVQANSAEFEPA
EEECCCCCCCCCCCCCCCEEEHHHCCCCHHHHHHCCEEEEEEECCCCEEEEECCCCCCCC
TPDPVISTMADQKEIVAGEADFGGTMRLGAYPAVLQPASIVAQAYGTTQVSERHRHRYEV
CCCHHHHHHCCCHHHEECCCCCCCEEEECCCHHHHCHHHHHHHHCCCHHHHHHHHHHHHH
NNAYRDWIAESGLRISGTSPDGYLVEFVEYPANMHPFVVGTQAHPELKSRPTRPHPLFVA
HHHHHHHHHHCCCEEECCCCCCCEEHEECCCCCCCCEEEECCCCCHHHCCCCCCCCEEEE
FVGAAIDYKSAELLPVEIPAVPEISEHLPNSSNQHRDGVERSFPAPAARG
EEHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MRKHPQSATKHLFVSGGVASSLGKGLTASSLGQLLTARGLHVTMQKLDPYLNVDPGTMNP
CCCCCCCHHCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCEEEHHHCCCCEECCCCCCCC
FQHGEVFVTEDGAETDLDVGHYERFLDRDLSGSANVTTGQVYSTVIAKERRGEYLGDTVQ
CCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
VIPHITDEIKQRIMAMAQPDGGDNRPDVVITEIGGTVGDIESQPFLEAARQVRHDLGREN
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
VFFLHVSLVPHLAPSGELKTKPTQHSVAALRSIGITPDALILRCDRDVPESLKNKIALMC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCEEEEE
DVDIDGVISTPDAPSIYDIPKVLHREELDAFVVRRLNLPFRDVDWTEWDDLLRRVHEPHG
ECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
TVRIALVGKYVDFSDAYLSVSEALHAGGFKHYAKVEVVWVASDDCETATGAAAVLADVHG
CEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCC
VLIPGGFGIRGIEGKIGAIRYARARGLPVLGLCLGLQCIVIEATRSVGLVQANSAEFEPA
EEECCCCCCCCCCCCCCCEEEHHHCCCCHHHHHHCCEEEEEEECCCCEEEEECCCCCCCC
TPDPVISTMADQKEIVAGEADFGGTMRLGAYPAVLQPASIVAQAYGTTQVSERHRHRYEV
CCCHHHHHHCCCHHHEECCCCCCCEEEECCCHHHHCHHHHHHHHCCCHHHHHHHHHHHHH
NNAYRDWIAESGLRISGTSPDGYLVEFVEYPANMHPFVVGTQAHPELKSRPTRPHPLFVA
HHHHHHHHHHCCCEEECCCCCCCEEHEECCCCCCCCEEEECCCCCHHHCCCCCCCCEEEE
FVGAAIDYKSAELLPVEIPAVPEISEHLPNSSNQHRDGVERSFPAPAARG
EEHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA