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The map label for this gene is trpA

Identifier: 221230132

GI number: 221230132

Start: 1516656

End: 1517468

Strand: Direct

Name: trpA

Synonym: MLBr_01273

Alternate gene names: 221230132

Gene position: 1516656-1517468 (Clockwise)

Preceding gene: 221230131

Following gene: 221230133

Centisome position: 46.41

GC content: 60.15

Gene sequence:

>813_bases
ATGATGGTTCTGGAACAGAGCGAAACAAGTAGGCTTGGGCCGGTTTTCGATTCGTGTCGTGCGGACAATCGCGCGGCATT
GATTGGTTACTTGCCCACCGGTTACCCTGATGTGCCTACTTCGGTGGACGCGATGATAGAACTCGTCGAATCCGGCTGTG
ACATCATTGAAGTTGGCGTTCCTTATTCGGATCCCGGTATGGACGGTCCAACCATCGCGAGGGCCACCGAGGTTGCGCTT
CGTGGAGGAGTACGAGTCCGCGATACTCTAGCCGCGGTCGAGGCGATCAGCCAAGCTGGCGGACGCGCCGTGGTGATGAC
GTACTGGAATCCTGTGTTGCGCTATGGGGTTTGTGCGTTTGCGCGGGATCTGGAGTCAGCTGGAGGACACGGTCTGGTCA
CTCCCGACCTTATTCCCGACGAAGCACGCCAATGGATCGCGGCATCCGAGAAGCATCGGTTGGATCGCATTTTTCTAGTG
GCTCCATCATCGACGCCGCACCGGTTGGTGACCACGGTCGGGGCATCACGTGGATTTGTTTATGCGGTGTCTGTGATGGG
TGTGACCGGGGCGCGTGACGCGGTGTCGCAGGTTGTGCCAGAACTGGTGGCCAGGGTGAAGGCAGTATCTGACATTGCGG
TCGGGGTCGGCCTGGGGGTGCGATCACGAGAGCAGGCCGCGCAGATCGGCGGCTACGCCGACGGTGTCATTGTTGGTTCC
GCGTTGGTGTCGGCGCTGGGTGACGGTTTGCCTCGATTGCGTGCTCTTGCCGAGGAACTCGCCGCCGGCGTGCGCCAAAG
GACGTTCATGTGA

Upstream 100 bases:

>100_bases
CTGAAATAGGCAAGGGTGCGGTCATAGTGGTGAACCTTTCGGGTCGCGGCGATAAGGATATCGAGGCAGCTGCTAAGTGG
TTCGGCCTGCTGGGTACCAG

Downstream 100 bases:

>100_bases
CGAGGATGTTGCCTGGATATTTTCCCAGCCCACCGCGTGGGGTCTGGCACCTTGGTCCGCTGCCGATTCGAGCGTACGCG
CTGCTCATCATCCTCGGTAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MMVLEQSETSRLGPVFDSCRADNRAALIGYLPTGYPDVPTSVDAMIELVESGCDIIEVGVPYSDPGMDGPTIARATEVAL
RGGVRVRDTLAAVEAISQAGGRAVVMTYWNPVLRYGVCAFARDLESAGGHGLVTPDLIPDEARQWIAASEKHRLDRIFLV
APSSTPHRLVTTVGASRGFVYAVSVMGVTGARDAVSQVVPELVARVKAVSDIAVGVGLGVRSREQAAQIGGYADGVIVGS
ALVSALGDGLPRLRALAEELAAGVRQRTFM

Sequences:

>Translated_270_residues
MMVLEQSETSRLGPVFDSCRADNRAALIGYLPTGYPDVPTSVDAMIELVESGCDIIEVGVPYSDPGMDGPTIARATEVAL
RGGVRVRDTLAAVEAISQAGGRAVVMTYWNPVLRYGVCAFARDLESAGGHGLVTPDLIPDEARQWIAASEKHRLDRIFLV
APSSTPHRLVTTVGASRGFVYAVSVMGVTGARDAVSQVVPELVARVKAVSDIAVGVGLGVRSREQAAQIGGYADGVIVGS
ALVSALGDGLPRLRALAEELAAGVRQRTFM
>Mature_270_residues
MMVLEQSETSRLGPVFDSCRADNRAALIGYLPTGYPDVPTSVDAMIELVESGCDIIEVGVPYSDPGMDGPTIARATEVAL
RGGVRVRDTLAAVEAISQAGGRAVVMTYWNPVLRYGVCAFARDLESAGGHGLVTPDLIPDEARQWIAASEKHRLDRIFLV
APSSTPHRLVTTVGASRGFVYAVSVMGVTGARDAVSQVVPELVARVKAVSDIAVGVGLGVRSREQAAQIGGYADGVIVGS
ALVSALGDGLPRLRALAEELAAGVRQRTFM

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=218, Percent_Identity=27.0642201834862, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321412, Length=246, Percent_Identity=39.4308943089431, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_MYCLB (B8ZRC1)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503548.1
- ProteinModelPortal:   B8ZRC1
- SMR:   B8ZRC1
- EnsemblBacteria:   EBMYCT00000085361
- GeneID:   7327281
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016358
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28331; Mature: 28331

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 57-57 ACT_SITE 68-68

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVLEQSETSRLGPVFDSCRADNRAALIGYLPTGYPDVPTSVDAMIELVESGCDIIEVGV
CCEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECC
PYSDPGMDGPTIARATEVALRGGVRVRDTLAAVEAISQAGGRAVVMTYWNPVLRYGVCAF
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
ARDLESAGGHGLVTPDLIPDEARQWIAASEKHRLDRIFLVAPSSTPHRLVTTVGASRGFV
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCEE
YAVSVMGVTGARDAVSQVVPELVARVKAVSDIAVGVGLGVRSREQAAQIGGYADGVIVGS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHH
ALVSALGDGLPRLRALAEELAAGVRQRTFM
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMVLEQSETSRLGPVFDSCRADNRAALIGYLPTGYPDVPTSVDAMIELVESGCDIIEVGV
CCEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECC
PYSDPGMDGPTIARATEVALRGGVRVRDTLAAVEAISQAGGRAVVMTYWNPVLRYGVCAF
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
ARDLESAGGHGLVTPDLIPDEARQWIAASEKHRLDRIFLVAPSSTPHRLVTTVGASRGFV
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCEE
YAVSVMGVTGARDAVSQVVPELVARVKAVSDIAVGVGLGVRSREQAAQIGGYADGVIVGS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHH
ALVSALGDGLPRLRALAEELAAGVRQRTFM
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA