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The map label for this gene is trpB

Identifier: 221230131

GI number: 221230131

Start: 1515406

End: 1516659

Strand: Direct

Name: trpB

Synonym: MLBr_01272

Alternate gene names: 221230131

Gene position: 1515406-1516659 (Clockwise)

Preceding gene: 221230130

Following gene: 221230132

Centisome position: 46.37

GC content: 59.17

Gene sequence:

>1254_bases
GTGATGCCGAATCTATCCTGTTTTAGCGTGGCCATCTCCGAACCCACTTGCCACGACCCCGATTCGGGTGGGCATTTTGG
TGGCCCCGATGGTTATGGTGGTCGCTACGTCCCCGAAGCGCTGATGGCGGTGATCGAGGAGGTCACCGCCGCTTACGAGA
AGGAACGCGTTAATCAGGACTTCCTGGACCTGCTGGACAAGTTGCAAGCAAACTACGCTGGCCGGCCGTCGCCGCTATAT
GAAGCTACTCGGCTTAGCGAATACGCTGGCTCGGTGCGCATCTTTCTCAAGCGGGAAGACTTGAACCACACTGGTTCTCA
CAAGATCAACAATGTCCTTGGTCAGACATTGCTGGCCCAGCGAATGGGCAAGACGCGAGTGATTGCTGAGACGGGTGCCG
GTCAGCATGGAGTGGCCACAGCGACGGCGTGTGCGCTGTTCGGCCTGGATTGTGTGATCTACATGGGTGCCCTTGACACT
GCTAGGCAGGCACTCAACGTGGCGCGGATGCGGTTGCTGGGTGCCGAGGTCGTATCGGTCGAGACTGGCTCGCGAACGCT
CAAGGACGCCATCAACGACGCGTTCCGGGATTGGGTTACTAATGCAGATAACACTTACTACTGCTTCGGGACTGCTTCGG
GACCGCATCCTTTTCCGACTATGGTGCGTGACTTACAGCGCGTCATCGGTCTCGAGACACGTCGGCAAATCCAGTATCAG
GCGGGCAGGCTACCGGACGCGGTTACGGCGTGCATCGGCGGAGGGTCCAACGCTATCGGGATCTTCCACGCGTTCCTCGA
TGATCCGGGTGTACGGCTGGTCGGGTTCGAGGCAGCTGGCGATGGCGTCGAGACTGGCAGGCATGCAGCGACATTGACCG
GCGGCTTGCCCGGAGCGTTCCAGGGAACGTTCTCCTACTTGCTGCAGGACGAGGACGGTCAGACCATCGAGTCCCATTCA
ATCGCAGCAGGTCTGGACTATCCGGGGGTTGGCCCCGAACACGCGTGGCTCAGGGAAACCGGACGCGCCGAATACCAGCC
CATTACTGATTCTGAGGCGATAGAGGCGTTTCGTTTACTGTGTCGCACCGAAGGTATTTTACCGGCCCTCGAGTCCGCGC
ATGCTGTAGCCGGTGCCCTCAAGTTGGCTTCTGAAATAGGCAAGGGTGCGGTCATAGTGGTGAACCTTTCGGGTCGCGGC
GATAAGGATATCGAGGCAGCTGCTAAGTGGTTCGGCCTGCTGGGTACCAGATGA

Upstream 100 bases:

>100_bases
GGGTACCCACCCGTCATGCCCAAAACCGGCTCGCTAGTCATTCGACAAGATGCAGTGGTGAAACATTGAACGCTATCAGC
CGACTGCCCGTTGAGTTCCG

Downstream 100 bases:

>100_bases
TGGTTCTGGAACAGAGCGAAACAAGTAGGCTTGGGCCGGTTTTCGATTCGTGTCGTGCGGACAATCGCGCGGCATTGATT
GGTTACTTGCCCACCGGTTA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MMPNLSCFSVAISEPTCHDPDSGGHFGGPDGYGGRYVPEALMAVIEEVTAAYEKERVNQDFLDLLDKLQANYAGRPSPLY
EATRLSEYAGSVRIFLKREDLNHTGSHKINNVLGQTLLAQRMGKTRVIAETGAGQHGVATATACALFGLDCVIYMGALDT
ARQALNVARMRLLGAEVVSVETGSRTLKDAINDAFRDWVTNADNTYYCFGTASGPHPFPTMVRDLQRVIGLETRRQIQYQ
AGRLPDAVTACIGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATLTGGLPGAFQGTFSYLLQDEDGQTIESHS
IAAGLDYPGVGPEHAWLRETGRAEYQPITDSEAIEAFRLLCRTEGILPALESAHAVAGALKLASEIGKGAVIVVNLSGRG
DKDIEAAAKWFGLLGTR

Sequences:

>Translated_417_residues
MMPNLSCFSVAISEPTCHDPDSGGHFGGPDGYGGRYVPEALMAVIEEVTAAYEKERVNQDFLDLLDKLQANYAGRPSPLY
EATRLSEYAGSVRIFLKREDLNHTGSHKINNVLGQTLLAQRMGKTRVIAETGAGQHGVATATACALFGLDCVIYMGALDT
ARQALNVARMRLLGAEVVSVETGSRTLKDAINDAFRDWVTNADNTYYCFGTASGPHPFPTMVRDLQRVIGLETRRQIQYQ
AGRLPDAVTACIGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATLTGGLPGAFQGTFSYLLQDEDGQTIESHS
IAAGLDYPGVGPEHAWLRETGRAEYQPITDSEAIEAFRLLCRTEGILPALESAHAVAGALKLASEIGKGAVIVVNLSGRG
DKDIEAAAKWFGLLGTR
>Mature_417_residues
MMPNLSCFSVAISEPTCHDPDSGGHFGGPDGYGGRYVPEALMAVIEEVTAAYEKERVNQDFLDLLDKLQANYAGRPSPLY
EATRLSEYAGSVRIFLKREDLNHTGSHKINNVLGQTLLAQRMGKTRVIAETGAGQHGVATATACALFGLDCVIYMGALDT
ARQALNVARMRLLGAEVVSVETGSRTLKDAINDAFRDWVTNADNTYYCFGTASGPHPFPTMVRDLQRVIGLETRRQIQYQ
AGRLPDAVTACIGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATLTGGLPGAFQGTFSYLLQDEDGQTIESHS
IAAGLDYPGVGPEHAWLRETGRAEYQPITDSEAIEAFRLLCRTEGILPALESAHAVAGALKLASEIGKGAVIVVNLSGRG
DKDIEAAAKWFGLLGTR

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=375, Percent_Identity=55.7333333333333, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321412, Length=387, Percent_Identity=55.5555555555556, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_MYCLE (Q9CC54)

Other databases:

- EMBL:   AL583921
- PIR:   B87068
- RefSeq:   NP_301917.1
- ProteinModelPortal:   Q9CC54
- SMR:   Q9CC54
- EnsemblBacteria:   EBMYCT00000028205
- GeneID:   910381
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1272
- NMPDR:   fig|272631.1.peg.789
- Leproma:   ML1272
- GeneTree:   EBGT00050000015765
- HOGENOM:   HBG303148
- OMA:   TFENTHY
- ProtClustDB:   PRK04346
- BioCyc:   MLEP272631:ML1272-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 44503; Mature: 44503

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPNLSCFSVAISEPTCHDPDSGGHFGGPDGYGGRYVPEALMAVIEEVTAAYEKERVNQD
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FLDLLDKLQANYAGRPSPLYEATRLSEYAGSVRIFLKREDLNHTGSHKINNVLGQTLLAQ
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RMGKTRVIAETGAGQHGVATATACALFGLDCVIYMGALDTARQALNVARMRLLGAEVVSV
HCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ETGSRTLKDAINDAFRDWVTNADNTYYCFGTASGPHPFPTMVRDLQRVIGLETRRQIQYQ
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHH
AGRLPDAVTACIGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATLTGGLPGAF
HCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCHH
QGTFSYLLQDEDGQTIESHSIAAGLDYPGVGPEHAWLRETGRAEYQPITDSEAIEAFRLL
HHHHHHHEECCCCCEECCCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH
CRTEGILPALESAHAVAGALKLASEIGKGAVIVVNLSGRGDKDIEAAAKWFGLLGTR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMPNLSCFSVAISEPTCHDPDSGGHFGGPDGYGGRYVPEALMAVIEEVTAAYEKERVNQD
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FLDLLDKLQANYAGRPSPLYEATRLSEYAGSVRIFLKREDLNHTGSHKINNVLGQTLLAQ
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RMGKTRVIAETGAGQHGVATATACALFGLDCVIYMGALDTARQALNVARMRLLGAEVVSV
HCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ETGSRTLKDAINDAFRDWVTNADNTYYCFGTASGPHPFPTMVRDLQRVIGLETRRQIQYQ
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHH
AGRLPDAVTACIGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATLTGGLPGAF
HCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCHH
QGTFSYLLQDEDGQTIESHSIAAGLDYPGVGPEHAWLRETGRAEYQPITDSEAIEAFRLL
HHHHHHHEECCCCCEECCCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH
CRTEGILPALESAHAVAGALKLASEIGKGAVIVVNLSGRGDKDIEAAAKWFGLLGTR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002