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The map label for this gene is dapE [H]

Identifier: 221230084

GI number: 221230084

Start: 1406391

End: 1407719

Strand: Direct

Name: dapE [H]

Synonym: MLBr_01193

Alternate gene names: 221230084

Gene position: 1406391-1407719 (Clockwise)

Preceding gene: 221230083

Following gene: 221230085

Centisome position: 43.03

GC content: 62.23

Gene sequence:

>1329_bases
ATGAGTGATCTTATTGAGCGTGTTCGTGAAGTGCTACCGTTGGTACGACGCGACCTTGAGAATTTGGTCCGGATCGAATC
GGTGTGGGCCGATCCTGGCCGGCGGAATGAGGTGCACCGCAGCGCGCAAGTGGTCTTAGATCTGTTGTCGCAAGCGGGTT
TTAACTTTGTGCGTATCGTCAGTGAGGGTGGTGCGCCGGCCGTCATTGCTCGGTATCCGGCCCCACTGGGAACGCCCACC
GTTTTGCTGTATGCCCACCATGACGTTCAGCCGGAAGGTGACCGCGACCAGTGGGCGTCGCCGCCTTTCGAGCCCACTGA
ACGCGACGGGCGAATCTACGGTCGCGGCAGCGCCGATGATAAAGCAGGCATTGCAACGCATTTAGCTGCGTTCCGGGCGC
ATGGTGGTCGACCGCCGGTGGGTGTGACAGTCTTCGTGGAGGGCGAAGAAGAATCCGGATCGCCGTCGCTGGGCAGGTTG
CTGGCCGCCCACCGCGATGCGCTGGCTGCTGACGTGATCATAATTGCCGACTCGGATAACTGGAGTACCGATGTCCCTGC
GCTGACGGTGTCACTGCGTGGCTTAGTCGACTGCGTCGTTGAGGTCGCCACACTTGACCACGGGCTGCATTCCGGATTGT
GGGGTGGCGTGGTTCCTGACGCGCTGAGTGTGCTGATGCGGCTGCTGGCCAGCCTGCACGACGACGACGGCAACGTTGCC
GTGGCGGGTCTGCATGAAAGTACTACTGCTGCAGACGTGAACTATCCGCACGAGCGAGTACGGGCTGACTCTGGCTTGTT
GGATGGGGTGTCAGAAATAGGCTCGGGTTCAGTGCCGCAGCGGCTATGGGCCAAACCGGCGATCACTGTGATTGGTATCG
ACACCACATCCGTTGCCGCAGCCTCGAACATGCTGATCCCGCGGGCCCGGGCCAAGATCAGCATGCGGATCGCGCCGGGC
GGTGACGCTGCGGTGCACCTGGATGCGTTGACCGCGCATCTGCAGCGGTATGCTCCGTGGGGTGCCCAAGTCAGCGTTAT
CCGCGGCGAGGTCAGCCAACCGTACGCCATCGAGGCCAGCGGCGGAGTTTATGACACCGCGCGGACAGCGTTCCGGCAGG
CGTGGGGTGCTGATCCGACCGATATGGGGATGGGTGGCTCGATCCCGTTCATCGCCGAGTTCGCCTCCGCGTTTCCGCAA
GCGAAGATCCTGGTCACCGGTGTCGGAGACCCTGCAACGCAGGCTCACGGCGTTAACGAGAGCGTGCATTTGGGTGTGTT
AGAACGCGCGGCGGTCGCTGAAGCGCTGTTGTTGGCCAACTTGGCCTGA

Upstream 100 bases:

>100_bases
AGGTTCTGTGAGCTGGGGTACCTCCCCCGTTTGTGGGGAGGTACCCCAGCCGCCTGCGGCGCAAGCTGGCGTGCAGTAGA
CGCTGGACTAGCCTCAAGCC

Downstream 100 bases:

>100_bases
GTGGCGTCAAAAATCGTGCGGTTCCCGGGCTCTGCTGTCTTGTGTCTGTGGCGGTGAGTTGTTCGGTTGGTTTCATAGGT
GGTGGGTGAAATGGCTGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSDLIERVREVLPLVRRDLENLVRIESVWADPGRRNEVHRSAQVVLDLLSQAGFNFVRIVSEGGAPAVIARYPAPLGTPT
VLLYAHHDVQPEGDRDQWASPPFEPTERDGRIYGRGSADDKAGIATHLAAFRAHGGRPPVGVTVFVEGEEESGSPSLGRL
LAAHRDALAADVIIIADSDNWSTDVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPDALSVLMRLLASLHDDDGNVA
VAGLHESTTAADVNYPHERVRADSGLLDGVSEIGSGSVPQRLWAKPAITVIGIDTTSVAAASNMLIPRARAKISMRIAPG
GDAAVHLDALTAHLQRYAPWGAQVSVIRGEVSQPYAIEASGGVYDTARTAFRQAWGADPTDMGMGGSIPFIAEFASAFPQ
AKILVTGVGDPATQAHGVNESVHLGVLERAAVAEALLLANLA

Sequences:

>Translated_442_residues
MSDLIERVREVLPLVRRDLENLVRIESVWADPGRRNEVHRSAQVVLDLLSQAGFNFVRIVSEGGAPAVIARYPAPLGTPT
VLLYAHHDVQPEGDRDQWASPPFEPTERDGRIYGRGSADDKAGIATHLAAFRAHGGRPPVGVTVFVEGEEESGSPSLGRL
LAAHRDALAADVIIIADSDNWSTDVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPDALSVLMRLLASLHDDDGNVA
VAGLHESTTAADVNYPHERVRADSGLLDGVSEIGSGSVPQRLWAKPAITVIGIDTTSVAAASNMLIPRARAKISMRIAPG
GDAAVHLDALTAHLQRYAPWGAQVSVIRGEVSQPYAIEASGGVYDTARTAFRQAWGADPTDMGMGGSIPFIAEFASAFPQ
AKILVTGVGDPATQAHGVNESVHLGVLERAAVAEALLLANLA
>Mature_441_residues
SDLIERVREVLPLVRRDLENLVRIESVWADPGRRNEVHRSAQVVLDLLSQAGFNFVRIVSEGGAPAVIARYPAPLGTPTV
LLYAHHDVQPEGDRDQWASPPFEPTERDGRIYGRGSADDKAGIATHLAAFRAHGGRPPVGVTVFVEGEEESGSPSLGRLL
AAHRDALAADVIIIADSDNWSTDVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPDALSVLMRLLASLHDDDGNVAV
AGLHESTTAADVNYPHERVRADSGLLDGVSEIGSGSVPQRLWAKPAITVIGIDTTSVAAASNMLIPRARAKISMRIAPGG
DAAVHLDALTAHLQRYAPWGAQVSVIRGEVSQPYAIEASGGVYDTARTAFRQAWGADPTDMGMGGSIPFIAEFASAFPQA
KILVTGVGDPATQAHGVNESVHLGVLERAAVAEALLLANLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=452, Percent_Identity=30.0884955752212, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI21071039, Length=460, Percent_Identity=28.2608695652174, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI271398277, Length=300, Percent_Identity=28.6666666666667, Blast_Score=116, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17563146, Length=434, Percent_Identity=28.5714285714286, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI193211059, Length=469, Percent_Identity=27.5053304904051, Blast_Score=147, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6321159, Length=468, Percent_Identity=26.9230769230769, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319758, Length=383, Percent_Identity=22.976501305483, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585880, Length=436, Percent_Identity=30.2752293577982, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 46615; Mature: 46484

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLIERVREVLPLVRRDLENLVRIESVWADPGRRNEVHRSAQVVLDLLSQAGFNFVRIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
SEGGAPAVIARYPAPLGTPTVLLYAHHDVQPEGDRDQWASPPFEPTERDGRIYGRGSADD
ECCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
KAGIATHLAAFRAHGGRPPVGVTVFVEGEEESGSPSLGRLLAAHRDALAADVIIIADSDN
CHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCEEEEEEEECCCC
WSTDVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPDALSVLMRLLASLHDDDGNVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VAGLHESTTAADVNYPHERVRADSGLLDGVSEIGSGSVPQRLWAKPAITVIGIDTTSVAA
EEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCHHHHCCCCEEEEECCCHHHHH
ASNMLIPRARAKISMRIAPGGDAAVHLDALTAHLQRYAPWGAQVSVIRGEVSQPYAIEAS
HHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECC
GGVYDTARTAFRQAWGADPTDMGMGGSIPFIAEFASAFPQAKILVTGVGDPATQAHGVNE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCC
SVHLGVLERAAVAEALLLANLA
CEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDLIERVREVLPLVRRDLENLVRIESVWADPGRRNEVHRSAQVVLDLLSQAGFNFVRIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
SEGGAPAVIARYPAPLGTPTVLLYAHHDVQPEGDRDQWASPPFEPTERDGRIYGRGSADD
ECCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
KAGIATHLAAFRAHGGRPPVGVTVFVEGEEESGSPSLGRLLAAHRDALAADVIIIADSDN
CHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCEEEEEEEECCCC
WSTDVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVVPDALSVLMRLLASLHDDDGNVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VAGLHESTTAADVNYPHERVRADSGLLDGVSEIGSGSVPQRLWAKPAITVIGIDTTSVAA
EEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCHHHHCCCCEEEEECCCHHHHH
ASNMLIPRARAKISMRIAPGGDAAVHLDALTAHLQRYAPWGAQVSVIRGEVSQPYAIEAS
HHCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECC
GGVYDTARTAFRQAWGADPTDMGMGGSIPFIAEFASAFPQAKILVTGVGDPATQAHGVNE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCC
SVHLGVLERAAVAEALLLANLA
CEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA