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The map label for this gene is thrS

Identifier: 221229625

GI number: 221229625

Start: 555671

End: 557779

Strand: Reverse

Name: thrS

Synonym: MLBr_00456

Alternate gene names: 221229625

Gene position: 557779-555671 (Counterclockwise)

Preceding gene: 221229626

Following gene: 221229624

Centisome position: 17.07

GC content: 59.36

Gene sequence:

>2109_bases
ATGAGCGCCCCCGTACACCCCGTCCCTGGAGCCGATGGCGGCGACCCGCTGCGCCCGGCCACGCCGGGCTTGCGGTCGCC
GCAAGTCCCAATCCAGGTTCCTGCCGGTAGTACCGCAGCGGCCGCGGTCAGCGAGGCCGGATTGCCAACACATGGCGCTC
CCGATGCGATTGTGGTGGTGCGCGACGCCGACGGGAAGTTACGCGACTTAAGCTGGGTTCCGGACGTCGACGTCGAGGTG
ACTCCGGTACCCGTCAATACCGACGACGGCCGCAGCGTGATCCGGCATTCGACGGCACACGTTTTGGCCCAAGCTGTCCA
AGACCTGTTCCCACAAGCCAAACTGGGGATCGGGCCGCCTATTACCGATGGCTTCTACTACGACTTCGACGTGGCCGAAC
CGTTCACGCCTGAAGATTTAAAGGCGCTAGAAAAGCGGATGCGGCAGATTGTCAAGGAAGGGCAGCTGTTCTCGCGACGG
ATCTACGAATCCAAGGAGCAAGCTCGTACCGAGTGGGCCGGCGAACCATACAAGCTGGAACTTGTCGACGACGAATCGGG
GGATGCCGAGATCATGGAGGTCGGCGGCGATGAGCTCACCGCTTATGACAACCTCAACGCCCGCAACGGGGAACGTATTT
GGGGTGACCTGTGCCGCGGACCACACATCCCAACTACCAAGCACATTCCTGCGTTCAAGCTGACCAGAAGTTCCGCTGCG
TACTGGCGAGGCAACCAGAAAAACGCCAGCCTGCAGCGCATCTATGGTACCGCGTGGGAATCGCAGGAGGCCCTCGATCG
CCACCTTGAGATGATTACCGAGGCCCAGCGCCGCGACCATCGCAAGCTGGGCATTGAGCTGGATCTGTTCAGTTTCCCCG
ACGAAATCGGTTCTGGCTTAGCGATTTTTCACCCTAAGGGGAGCATCGTACGTCGGGAAATGGAAGAGTACTCCCGGCGC
AAGCACATCGAAGCCGGCTACCAATTCGTCAACACCCCGCATATTACCAAGGCGCAGCTGTTCCACACTTCGGGTCACCT
CGACTGGTACGCCGAGGGTATATTCCCCCCGATGCACCTCGACGCCGAACACAACGATGACGGCACGGTGCGTAAGCCAG
GGCAAGACTACTACCTCAAGCCAATGAACTGCCCGATGCACACCCTGATTTTCTCCTCGCGCGGACGTTCGTATCGAGAG
TTGCCACTACGGCTCTTCGAGTTCGGCACGATCTACCGCTACGAGAAATCTGGTGTAGTACATGGGCTTACCCGTGCCCG
CGGGTTCACCATGGACGACTCGCACATCTTCTGCACCCGCGAGCAGCTGCACTGCGAGCTGGCGTCGCTGCTCCGGTTCG
TCCTGGATCTTCTTGGCGACTATGGGCTAGAAGATTTTTACCTCGAGCTTTCTACTAAAGACCCGGAGAAATTTGTTGGC
TCCGAAGAGATCTGGGAAGAAGCCACAGCCGCGCTGGCGGAGGTAGCCGAGAATTCTACTCTCCCATTGGTTCCCGATCC
AGGTGGCGCCGCGTTCTACGGCCCGAAGATCTCGGTGCAGGTGCGCGACGCACTGGGTCGCAGCTGGCAGATGTCCACCA
TCCAGGTAGACTTCAACTTTCCCGAACGGTTCGCATTGGAGTACACCTCCGCGGACGGGACTCGGCAGCGCCCAGTCATG
ATTCACCGGGCGTTATTCGGATCAATCGAGCGATTCTTCGGCATCCTCACCGAACACTATGCGGGAGCATTCCCTGCCTG
GCTAGCGCCCATTCAGGTGGTCGGTATACCGGTCACCGGTGAGCATGTATCTTACTTGGAAGAGGTTGCTGCGCAACTGA
AATCGTGTGGTGTCCGGACGGAGGTGGATGTCAGCGACGATCGGATGGCAAAGAAAATCGTCCGTCACACCAACCAGAAA
GTGCCGTTTATGTTACTGGCCGGCGACCGCGACGTGCGGACCGGGTCGGTGAGCTTCCGCTTTGGTGACCGCACACAGAT
CAACGGCGTCGCCCGCGACAGTGCCGTCGAGGCCATCGTTTGTTGGATCGTTGACCGCGAGAATGATTTTCCCACAGCAG
AATTGGTGAAAGTAACCGGCGGTGAGTGA

Upstream 100 bases:

>100_bases
GGCTTGGTTTCTTCAAAGCCCGCCCCGATAGCATGGCAACCGTTATCAGTTATGTCCCCAATTACAACCCGGCTACAAAT
TCGTCTTGGAGACCAACCCG

Downstream 100 bases:

>100_bases
GCAGGAAAGACCCGACACACGTGCCGATCACACAGACGGCACCATCTTGGACATGGGCGTCGGCGAGCGCGACCACTTGC
AACGGCTGTGGACACCGTAC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MSAPVHPVPGADGGDPLRPATPGLRSPQVPIQVPAGSTAAAAVSEAGLPTHGAPDAIVVVRDADGKLRDLSWVPDVDVEV
TPVPVNTDDGRSVIRHSTAHVLAQAVQDLFPQAKLGIGPPITDGFYYDFDVAEPFTPEDLKALEKRMRQIVKEGQLFSRR
IYESKEQARTEWAGEPYKLELVDDESGDAEIMEVGGDELTAYDNLNARNGERIWGDLCRGPHIPTTKHIPAFKLTRSSAA
YWRGNQKNASLQRIYGTAWESQEALDRHLEMITEAQRRDHRKLGIELDLFSFPDEIGSGLAIFHPKGSIVRREMEEYSRR
KHIEAGYQFVNTPHITKAQLFHTSGHLDWYAEGIFPPMHLDAEHNDDGTVRKPGQDYYLKPMNCPMHTLIFSSRGRSYRE
LPLRLFEFGTIYRYEKSGVVHGLTRARGFTMDDSHIFCTREQLHCELASLLRFVLDLLGDYGLEDFYLELSTKDPEKFVG
SEEIWEEATAALAEVAENSTLPLVPDPGGAAFYGPKISVQVRDALGRSWQMSTIQVDFNFPERFALEYTSADGTRQRPVM
IHRALFGSIERFFGILTEHYAGAFPAWLAPIQVVGIPVTGEHVSYLEEVAAQLKSCGVRTEVDVSDDRMAKKIVRHTNQK
VPFMLLAGDRDVRTGSVSFRFGDRTQINGVARDSAVEAIVCWIVDRENDFPTAELVKVTGGE

Sequences:

>Translated_702_residues
MSAPVHPVPGADGGDPLRPATPGLRSPQVPIQVPAGSTAAAAVSEAGLPTHGAPDAIVVVRDADGKLRDLSWVPDVDVEV
TPVPVNTDDGRSVIRHSTAHVLAQAVQDLFPQAKLGIGPPITDGFYYDFDVAEPFTPEDLKALEKRMRQIVKEGQLFSRR
IYESKEQARTEWAGEPYKLELVDDESGDAEIMEVGGDELTAYDNLNARNGERIWGDLCRGPHIPTTKHIPAFKLTRSSAA
YWRGNQKNASLQRIYGTAWESQEALDRHLEMITEAQRRDHRKLGIELDLFSFPDEIGSGLAIFHPKGSIVRREMEEYSRR
KHIEAGYQFVNTPHITKAQLFHTSGHLDWYAEGIFPPMHLDAEHNDDGTVRKPGQDYYLKPMNCPMHTLIFSSRGRSYRE
LPLRLFEFGTIYRYEKSGVVHGLTRARGFTMDDSHIFCTREQLHCELASLLRFVLDLLGDYGLEDFYLELSTKDPEKFVG
SEEIWEEATAALAEVAENSTLPLVPDPGGAAFYGPKISVQVRDALGRSWQMSTIQVDFNFPERFALEYTSADGTRQRPVM
IHRALFGSIERFFGILTEHYAGAFPAWLAPIQVVGIPVTGEHVSYLEEVAAQLKSCGVRTEVDVSDDRMAKKIVRHTNQK
VPFMLLAGDRDVRTGSVSFRFGDRTQINGVARDSAVEAIVCWIVDRENDFPTAELVKVTGGE
>Mature_701_residues
SAPVHPVPGADGGDPLRPATPGLRSPQVPIQVPAGSTAAAAVSEAGLPTHGAPDAIVVVRDADGKLRDLSWVPDVDVEVT
PVPVNTDDGRSVIRHSTAHVLAQAVQDLFPQAKLGIGPPITDGFYYDFDVAEPFTPEDLKALEKRMRQIVKEGQLFSRRI
YESKEQARTEWAGEPYKLELVDDESGDAEIMEVGGDELTAYDNLNARNGERIWGDLCRGPHIPTTKHIPAFKLTRSSAAY
WRGNQKNASLQRIYGTAWESQEALDRHLEMITEAQRRDHRKLGIELDLFSFPDEIGSGLAIFHPKGSIVRREMEEYSRRK
HIEAGYQFVNTPHITKAQLFHTSGHLDWYAEGIFPPMHLDAEHNDDGTVRKPGQDYYLKPMNCPMHTLIFSSRGRSYREL
PLRLFEFGTIYRYEKSGVVHGLTRARGFTMDDSHIFCTREQLHCELASLLRFVLDLLGDYGLEDFYLELSTKDPEKFVGS
EEIWEEATAALAEVAENSTLPLVPDPGGAAFYGPKISVQVRDALGRSWQMSTIQVDFNFPERFALEYTSADGTRQRPVMI
HRALFGSIERFFGILTEHYAGAFPAWLAPIQVVGIPVTGEHVSYLEEVAAQLKSCGVRTEVDVSDDRMAKKIVRHTNQKV
PFMLLAGDRDVRTGSVSFRFGDRTQINGVARDSAVEAIVCWIVDRENDFPTAELVKVTGGE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=625, Percent_Identity=34.56, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI38202255, Length=635, Percent_Identity=34.9606299212598, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI20070344, Length=630, Percent_Identity=34.7619047619048, Blast_Score=336, Evalue=5e-92,
Organism=Escherichia coli, GI1788013, Length=612, Percent_Identity=40.0326797385621, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71984184, Length=634, Percent_Identity=34.0694006309148, Blast_Score=360, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71984192, Length=634, Percent_Identity=34.0694006309148, Blast_Score=360, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6322112, Length=641, Percent_Identity=36.5054602184087, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322654, Length=401, Percent_Identity=34.6633416458853, Blast_Score=243, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24583842, Length=665, Percent_Identity=35.6390977443609, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28574141, Length=665, Percent_Identity=35.6390977443609, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24583839, Length=665, Percent_Identity=35.6390977443609, Blast_Score=397, Evalue=1e-110,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_MYCLB (B8ZUG9)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503041.1
- ProteinModelPortal:   B8ZUG9
- SMR:   B8ZUG9
- EnsemblBacteria:   EBMYCT00000086418
- GeneID:   7325650
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00070000032298
- HOGENOM:   HBG352811
- OMA:   NDETMST
- ProtClustDB:   PRK12305
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 78373; Mature: 78242

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVHPVPGADGGDPLRPATPGLRSPQVPIQVPAGSTAAAAVSEAGLPTHGAPDAIVVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCEEEEE
RDADGKLRDLSWVPDVDVEVTPVPVNTDDGRSVIRHSTAHVLAQAVQDLFPQAKLGIGPP
ECCCCCEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ITDGFYYDFDVAEPFTPEDLKALEKRMRQIVKEGQLFSRRIYESKEQARTEWAGEPYKLE
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LVDDESGDAEIMEVGGDELTAYDNLNARNGERIWGDLCRGPHIPTTKHIPAFKLTRSSAA
EEECCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCH
YWRGNQKNASLQRIYGTAWESQEALDRHLEMITEAQRRDHRKLGIELDLFSFPDEIGSGL
HCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCE
AIFHPKGSIVRREMEEYSRRKHIEAGYQFVNTPHITKAQLFHTSGHLDWYAEGIFPPMHL
EEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCCEEEECCCCCCEEE
DAEHNDDGTVRKPGQDYYLKPMNCPMHTLIFSSRGRSYRELPLRLFEFGTIYRYEKSGVV
CCCCCCCCCEECCCCCCEEECCCCCHHEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCH
HGLTRARGFTMDDSHIFCTREQLHCELASLLRFVLDLLGDYGLEDFYLELSTKDPEKFVG
HHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCC
SEEIWEEATAALAEVAENSTLPLVPDPGGAAFYGPKISVQVRDALGRSWQMSTIQVDFNF
CHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEECCEEEEEEHHHCCCCEEEEEEEEEECC
PERFALEYTSADGTRQRPVMIHRALFGSIERFFGILTEHYAGAFPAWLAPIQVVGIPVTG
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEECCC
EHVSYLEEVAAQLKSCGVRTEVDVSDDRMAKKIVRHTNQKVPFMLLAGDRDVRTGSVSFR
HHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECEEEEE
FGDRTQINGVARDSAVEAIVCWIVDRENDFPTAELVKVTGGE
ECCCCEECCCHHHHHHEEEEEEEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SAPVHPVPGADGGDPLRPATPGLRSPQVPIQVPAGSTAAAAVSEAGLPTHGAPDAIVVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCEEEEE
RDADGKLRDLSWVPDVDVEVTPVPVNTDDGRSVIRHSTAHVLAQAVQDLFPQAKLGIGPP
ECCCCCEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ITDGFYYDFDVAEPFTPEDLKALEKRMRQIVKEGQLFSRRIYESKEQARTEWAGEPYKLE
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LVDDESGDAEIMEVGGDELTAYDNLNARNGERIWGDLCRGPHIPTTKHIPAFKLTRSSAA
EEECCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCH
YWRGNQKNASLQRIYGTAWESQEALDRHLEMITEAQRRDHRKLGIELDLFSFPDEIGSGL
HCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCE
AIFHPKGSIVRREMEEYSRRKHIEAGYQFVNTPHITKAQLFHTSGHLDWYAEGIFPPMHL
EEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCCEEEECCCCCCEEE
DAEHNDDGTVRKPGQDYYLKPMNCPMHTLIFSSRGRSYRELPLRLFEFGTIYRYEKSGVV
CCCCCCCCCEECCCCCCEEECCCCCHHEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCH
HGLTRARGFTMDDSHIFCTREQLHCELASLLRFVLDLLGDYGLEDFYLELSTKDPEKFVG
HHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCC
SEEIWEEATAALAEVAENSTLPLVPDPGGAAFYGPKISVQVRDALGRSWQMSTIQVDFNF
CHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEECCEEEEEEHHHCCCCEEEEEEEEEECC
PERFALEYTSADGTRQRPVMIHRALFGSIERFFGILTEHYAGAFPAWLAPIQVVGIPVTG
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEECCC
EHVSYLEEVAAQLKSCGVRTEVDVSDDRMAKKIVRHTNQKVPFMLLAGDRDVRTGSVSFR
HHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECEEEEE
FGDRTQINGVARDSAVEAIVCWIVDRENDFPTAELVKVTGGE
ECCCCEECCCHHHHHHEEEEEEEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA