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The map label for this gene is pimA

Identifier: 221229621

GI number: 221229621

Start: 552249

End: 553373

Strand: Reverse

Name: pimA

Synonym: MLBr_00452

Alternate gene names: 221229621

Gene position: 553373-552249 (Counterclockwise)

Preceding gene: 221229622

Following gene: 221229620

Centisome position: 16.93

GC content: 63.2

Gene sequence:

>1125_bases
ATGCGGATCGGGATGATCTGTCCATACTCGTTCGATGTGCCAGGCGGGGTGCAATCGCACGTCTTGCAACTGGCCGAGGT
GATGCGCGCGCGGGGACAGCAGGTCCGTGTGCTGGCGCCGGCTTCGCCGGATGTCTCGCTGCCCGAATATGTTGTCTCCG
CCGGCAGGGCGATCCCGATTCCCTACAACGGTTCAGTGGCGCGGCTGCAGTTCAGTCCGGCAGTACACAGCAGAGTCAGG
CGTTGGTTAGTGGACGGCGATTTTGACGTGCTTCACCTGCACGAACCGAACGCACCCAGCCTGTCGATGTGGGCGCTGCG
GGTGGCCGAGGGGCCAATCGTCGCGACATTTCATACCTCCACCACCAAATCACTGACGCTGTCGGTATTCCAAGGCGTGC
TGCGACCATGGCACGAGAAGATCATCGGCCGGATCGCAGTATCCGACTTAGCACGGCGCTGGCAAATGGAGGCTCTGGGG
TCCGATGCGGTGGAGATCCCCAACGGGGTGAACGTTGATTCGTTGTCCTCGGCACCCCAGCTAGCCGGGTACCCGCGGCT
CGGCAAGACGGTACTGTTCCTCGGCCGCTACGACGAGCCACGCAAAGGCATGTCGGTCCTGCTTGATGCCCTACCGGGAG
TTATGGAATGCTTCGACGACGTACAGTTACTGATCGTCGGCCGTGGCGACGAAGAGCAATTGCGGAGCCAGGCAGGCGGA
TTGGTGGAGCACATCCGTTTTCTTGGCCAGGTGGATGACGCCGGAAAAGCCGCGGCGATGCGCAGCGCCGACGTCTATTG
CGCGCCCAACATCGGCGGCGAGAGTTTCGGCATTGTCCTGGTGGAGGCGATGGCCGCCGGCACTCCGGTGGTGGCGAGTG
ATCTGGACGCGTTTCGCCGCGTACTGCGCGACGGCGAAGTCGGGCATTTAGTGCCTGCGGGCGATAGCGCCGCGTTGGCC
GATGCGCTTGTCGCGTTGCTGCGCAACGACGTCCTGCGGGAGCGTTACGTGGCCGCCGGCGCCGAAGCGGTTCGTCGGTA
TGACTGGTCGGTGGTGGCCAGCCAAATCATGCGGGTGTACGAAACGGTCGCTACGTCAGGTTCCAAGGTGCAGGTGGCTA
GCTGA

Upstream 100 bases:

>100_bases
CGCCGCCAACCCCACCGACTGGCACATGCTTCAGCCGCAATGGTTGGCTGACTTGTCCGATGCCAAGCGAGCGCAGCTGA
GACCGAATTGAGGGAAGCCT

Downstream 100 bases:

>100_bases
TGCTGACCCTCTTGGTGCTGTTAGTGGCGCTGGCCACGTTGGCGGGCGGCTGGGGATATCAAACGGCAAATCGGCTGAAC
CGATTGCACGTCCGCTACGA

Product: putative glycosyltransferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRIGMICPYSFDVPGGVQSHVLQLAEVMRARGQQVRVLAPASPDVSLPEYVVSAGRAIPIPYNGSVARLQFSPAVHSRVR
RWLVDGDFDVLHLHEPNAPSLSMWALRVAEGPIVATFHTSTTKSLTLSVFQGVLRPWHEKIIGRIAVSDLARRWQMEALG
SDAVEIPNGVNVDSLSSAPQLAGYPRLGKTVLFLGRYDEPRKGMSVLLDALPGVMECFDDVQLLIVGRGDEEQLRSQAGG
LVEHIRFLGQVDDAGKAAAMRSADVYCAPNIGGESFGIVLVEAMAAGTPVVASDLDAFRRVLRDGEVGHLVPAGDSAALA
DALVALLRNDVLRERYVAAGAEAVRRYDWSVVASQIMRVYETVATSGSKVQVAS

Sequences:

>Translated_374_residues
MRIGMICPYSFDVPGGVQSHVLQLAEVMRARGQQVRVLAPASPDVSLPEYVVSAGRAIPIPYNGSVARLQFSPAVHSRVR
RWLVDGDFDVLHLHEPNAPSLSMWALRVAEGPIVATFHTSTTKSLTLSVFQGVLRPWHEKIIGRIAVSDLARRWQMEALG
SDAVEIPNGVNVDSLSSAPQLAGYPRLGKTVLFLGRYDEPRKGMSVLLDALPGVMECFDDVQLLIVGRGDEEQLRSQAGG
LVEHIRFLGQVDDAGKAAAMRSADVYCAPNIGGESFGIVLVEAMAAGTPVVASDLDAFRRVLRDGEVGHLVPAGDSAALA
DALVALLRNDVLRERYVAAGAEAVRRYDWSVVASQIMRVYETVATSGSKVQVAS
>Mature_374_residues
MRIGMICPYSFDVPGGVQSHVLQLAEVMRARGQQVRVLAPASPDVSLPEYVVSAGRAIPIPYNGSVARLQFSPAVHSRVR
RWLVDGDFDVLHLHEPNAPSLSMWALRVAEGPIVATFHTSTTKSLTLSVFQGVLRPWHEKIIGRIAVSDLARRWQMEALG
SDAVEIPNGVNVDSLSSAPQLAGYPRLGKTVLFLGRYDEPRKGMSVLLDALPGVMECFDDVQLLIVGRGDEEQLRSQAGG
LVEHIRFLGQVDDAGKAAAMRSADVYCAPNIGGESFGIVLVEAMAAGTPVVASDLDAFRRVLRDGEVGHLVPAGDSAALA
DALVALLRNDVLRERYVAAGAEAVRRYDWSVVASQIMRVYETVATSGSKVQVAS

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1)

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMA_MYCLE (O07147)

Other databases:

- EMBL:   Z96801
- EMBL:   AL583918
- PIR:   D86965
- RefSeq:   NP_301406.1
- ProteinModelPortal:   O07147
- SMR:   O07147
- EnsemblBacteria:   EBMYCT00000029219
- GeneID:   909199
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0452
- NMPDR:   fig|272631.1.peg.278
- Leproma:   ML0452
- GeneTree:   EBGT00050000015521
- HOGENOM:   HBG726846
- OMA:   VLHIHEP
- ProtClustDB:   CLSK872083
- BioCyc:   MLEP272631:ML0452-MONOMER
- BRENDA:   2.4.1.57
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: =2.4.1.57

Molecular weight: Translated: 40274; Mature: 40274

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: BINDING 9-9 BINDING 16-16 BINDING 196-196 BINDING 256-256 BINDING 282-282

Signals:

None

Transmembrane regions:

HASH(0x229fb43c)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGMICPYSFDVPGGVQSHVLQLAEVMRARGQQVRVLAPASPDVSLPEYVVSAGRAIPI
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCEEEC
PYNGSVARLQFSPAVHSRVRRWLVDGDFDVLHLHEPNAPSLSMWALRVAEGPIVATFHTS
CCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHEEEECCCCEEEEEECC
TTKSLTLSVFQGVLRPWHEKIIGRIAVSDLARRWQMEALGSDAVEIPNGVNVDSLSSAPQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCC
LAGYPRLGKTVLFLGRYDEPRKGMSVLLDALPGVMECFDDVQLLIVGRGDEEQLRSQAGG
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCH
LVEHIRFLGQVDDAGKAAAMRSADVYCAPNIGGESFGIVLVEAMAAGTPVVASDLDAFRR
HHHHHHHHHCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHEEHHHCCCCHHHHHHHHHHH
VLRDGEVGHLVPAGDSAALADALVALLRNDVLRERYVAAGAEAVRRYDWSVVASQIMRVY
HHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETVATSGSKVQVAS
HHHHCCCCEEEECH
>Mature Secondary Structure
MRIGMICPYSFDVPGGVQSHVLQLAEVMRARGQQVRVLAPASPDVSLPEYVVSAGRAIPI
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCEEEC
PYNGSVARLQFSPAVHSRVRRWLVDGDFDVLHLHEPNAPSLSMWALRVAEGPIVATFHTS
CCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHEEEECCCCEEEEEECC
TTKSLTLSVFQGVLRPWHEKIIGRIAVSDLARRWQMEALGSDAVEIPNGVNVDSLSSAPQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCC
LAGYPRLGKTVLFLGRYDEPRKGMSVLLDALPGVMECFDDVQLLIVGRGDEEQLRSQAGG
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCH
LVEHIRFLGQVDDAGKAAAMRSADVYCAPNIGGESFGIVLVEAMAAGTPVVASDLDAFRR
HHHHHHHHHCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHEEHHHCCCCHHHHHHHHHHH
VLRDGEVGHLVPAGDSAALADALVALLRNDVLRERYVAAGAEAVRRYDWSVVASQIMRVY
HHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETVATSGSKVQVAS
HHHHCCCCEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002