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The map label for this gene is tnaA [H]

Identifier: 220932922

GI number: 220932922

Start: 2263931

End: 2265319

Strand: Direct

Name: tnaA [H]

Synonym: Hore_20900

Alternate gene names: 220932922

Gene position: 2263931-2265319 (Clockwise)

Preceding gene: 220932921

Following gene: 220932923

Centisome position: 87.81

GC content: 42.33

Gene sequence:

>1389_bases
TTGAAAAAACAAATTAAACCTGAACCCTTTAAAATCAAGATGGTAGAACCTATTAACCTGATTCCAAAGGAAGAAAGAAA
AAAAGCACTGGAAAGAGCAGGCTATAACCTCTTTGCTTTAAAATCAAATGAAGTTTATATCGATCTCCTCACCGACAGTG
GTACCGGAGCAATGAGTGATAATCAGTGGGCAGGACTAATGCTGGGTGATGAATCCTATGCCGGAAGCCGTAACTATTTC
CACCTGGAAGAAACTGTAAAAGATATTTTTAATTACGATTATACTATTCCTACCCACCAGGGCCGCGGGGCAGAACAGGT
TCTCTTCCCCCTATTAATTAAGGAGAAAGGACAGTATGTTTTAAGTAATATGCATTTTGATACCACAAAAGCCCACGTTG
AACTGGCCGGGGGACGAGCCCGGAACTTTGTAATTGAAGAATCACGGGATACAACAATCTACCACCCCTTTAAAGGGAAT
TTTGATATCGAAAAGATGGAAGGTTTTATTAAAGACAAGGGTGCTGAAAACGTCGCTTTTATAATTATTACTATCACCTG
TAACAGTGCCGGTGGTCAACCTGTGTCCATGAAAAATATAAAAGAAGTCAGTGAAGTTGCTAAAAAATATGGAATCAGGG
TCTTTATCGATGCTGCCCGTTATGCTGAAAATGCCTACTTTATTAAACAGAGAGAAGAAGGGTATGCCGACAAGAGCATC
AAAGAAATCGTCCAGGAAATGTTCAGCTATGCAGAAGGTTTTACCATGAGTGCTAAAAAGGACGCCATTGTTAATATGGG
TGGTTTAATCGGTATCAAGGATAATGAAGACTTATACACCCAGGTTCGCTCCCGGGTTGTACCTCTTGAAGGTTTCCCGA
CATACGGTGGTCTATCCGGAAGGGATATGGAAGCCCTGGCCCGGGGACTTAAAGAAGGTATAGAATTAAGTTATCTTGAA
TATAGAATTGGCCAGGTCGAATATCTGGGAGAACAGTTGAGGGAAGCTGGAATCCCCATTCAGTATCCGACCGGTGGTCA
TGCTGTCTTTGTAGATGCCAAAAAAATGTTGCCCCATATCCCCTATTATCAGTTCCCGGCCCAGGCCCTGGGTAATGCCC
TTTATCTGGAATCAGGAGTCCGGGGAGTAGAAATTGGCTCCTTCCTCCTGGGTAGAGATCCAGAGACAGGTGAGCAATTA
GAATCTCCCTTTGAACTACTTCGCCTGACTATTCCAAGGCGGGTTTACACCAATAATCATATGGACTTTGTCGCCCAGGG
ATTAATTAAAATCAAAGATAAAGCTGAAGAATTAAAAGGACTGGAGTTCACCTATGAACCTGAAATTTTAAGACACTTTA
CTGCCAGATTAAAACCTGTTGAGGGCTAG

Upstream 100 bases:

>100_bases
ACTATTTAGATGAGTCCAGGGTGAGTGCTAGCAGAGAGTAGATGCAGCGGTAATAAAGGTCAGGGTCAAAAAATATTAAA
AAATTTAGGAGGGAATTACT

Downstream 100 bases:

>100_bases
GTCATAGTTTATTTACTCCCGGGGCCTACTCCGGGCCCCGGGACTATATTACTTCTATACTATATTGCTTCTATACTATA
TTCCTGTACTATATTACTTC

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKKQIKPEPFKIKMVEPINLIPKEERKKALERAGYNLFALKSNEVYIDLLTDSGTGAMSDNQWAGLMLGDESYAGSRNYF
HLEETVKDIFNYDYTIPTHQGRGAEQVLFPLLIKEKGQYVLSNMHFDTTKAHVELAGGRARNFVIEESRDTTIYHPFKGN
FDIEKMEGFIKDKGAENVAFIIITITCNSAGGQPVSMKNIKEVSEVAKKYGIRVFIDAARYAENAYFIKQREEGYADKSI
KEIVQEMFSYAEGFTMSAKKDAIVNMGGLIGIKDNEDLYTQVRSRVVPLEGFPTYGGLSGRDMEALARGLKEGIELSYLE
YRIGQVEYLGEQLREAGIPIQYPTGGHAVFVDAKKMLPHIPYYQFPAQALGNALYLESGVRGVEIGSFLLGRDPETGEQL
ESPFELLRLTIPRRVYTNNHMDFVAQGLIKIKDKAEELKGLEFTYEPEILRHFTARLKPVEG

Sequences:

>Translated_462_residues
MKKQIKPEPFKIKMVEPINLIPKEERKKALERAGYNLFALKSNEVYIDLLTDSGTGAMSDNQWAGLMLGDESYAGSRNYF
HLEETVKDIFNYDYTIPTHQGRGAEQVLFPLLIKEKGQYVLSNMHFDTTKAHVELAGGRARNFVIEESRDTTIYHPFKGN
FDIEKMEGFIKDKGAENVAFIIITITCNSAGGQPVSMKNIKEVSEVAKKYGIRVFIDAARYAENAYFIKQREEGYADKSI
KEIVQEMFSYAEGFTMSAKKDAIVNMGGLIGIKDNEDLYTQVRSRVVPLEGFPTYGGLSGRDMEALARGLKEGIELSYLE
YRIGQVEYLGEQLREAGIPIQYPTGGHAVFVDAKKMLPHIPYYQFPAQALGNALYLESGVRGVEIGSFLLGRDPETGEQL
ESPFELLRLTIPRRVYTNNHMDFVAQGLIKIKDKAEELKGLEFTYEPEILRHFTARLKPVEG
>Mature_462_residues
MKKQIKPEPFKIKMVEPINLIPKEERKKALERAGYNLFALKSNEVYIDLLTDSGTGAMSDNQWAGLMLGDESYAGSRNYF
HLEETVKDIFNYDYTIPTHQGRGAEQVLFPLLIKEKGQYVLSNMHFDTTKAHVELAGGRARNFVIEESRDTTIYHPFKGN
FDIEKMEGFIKDKGAENVAFIIITITCNSAGGQPVSMKNIKEVSEVAKKYGIRVFIDAARYAENAYFIKQREEGYADKSI
KEIVQEMFSYAEGFTMSAKKDAIVNMGGLIGIKDNEDLYTQVRSRVVPLEGFPTYGGLSGRDMEALARGLKEGIELSYLE
YRIGQVEYLGEQLREAGIPIQYPTGGHAVFVDAKKMLPHIPYYQFPAQALGNALYLESGVRGVEIGSFLLGRDPETGEQL
ESPFELLRLTIPRRVYTNNHMDFVAQGLIKIKDKAEELKGLEFTYEPEILRHFTARLKPVEG

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=465, Percent_Identity=54.6236559139785, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.1 [H]

Molecular weight: Translated: 52185; Mature: 52185

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQIKPEPFKIKMVEPINLIPKEERKKALERAGYNLFALKSNEVYIDLLTDSGTGAMSD
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCCC
NQWAGLMLGDESYAGSRNYFHLEETVKDIFNYDYTIPTHQGRGAEQVLFPLLIKEKGQYV
CCEEEEEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
LSNMHFDTTKAHVELAGGRARNFVIEESRDTTIYHPFKGNFDIEKMEGFIKDKGAENVAF
EECCCCCCCEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEE
IIITITCNSAGGQPVSMKNIKEVSEVAKKYGIRVFIDAARYAENAYFIKQREEGYADKSI
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCCEEEEEECCCCCCCHHH
KEIVQEMFSYAEGFTMSAKKDAIVNMGGLIGIKDNEDLYTQVRSRVVPLEGFPTYGGLSG
HHHHHHHHHHHCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHHCEECCCCCCCCCCCCC
RDMEALARGLKEGIELSYLEYRIGQVEYLGEQLREAGIPIQYPTGGHAVFVDAKKMLPHI
CCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEHHHHCCCC
PYYQFPAQALGNALYLESGVRGVEIGSFLLGRDPETGEQLESPFELLRLTIPRRVYTNNH
CCCCCCHHHHCCEEEECCCCCEEEECHHEECCCCCCHHHHHHHHHHHHHHCCCCEECCCC
MDFVAQGLIKIKDKAEELKGLEFTYEPEILRHFTARLKPVEG
HHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKQIKPEPFKIKMVEPINLIPKEERKKALERAGYNLFALKSNEVYIDLLTDSGTGAMSD
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCCC
NQWAGLMLGDESYAGSRNYFHLEETVKDIFNYDYTIPTHQGRGAEQVLFPLLIKEKGQYV
CCEEEEEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
LSNMHFDTTKAHVELAGGRARNFVIEESRDTTIYHPFKGNFDIEKMEGFIKDKGAENVAF
EECCCCCCCEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEE
IIITITCNSAGGQPVSMKNIKEVSEVAKKYGIRVFIDAARYAENAYFIKQREEGYADKSI
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCCEEEEEECCCCCCCHHH
KEIVQEMFSYAEGFTMSAKKDAIVNMGGLIGIKDNEDLYTQVRSRVVPLEGFPTYGGLSG
HHHHHHHHHHHCCCCCCCCCCCEEECCCEEEECCCHHHHHHHHHCEECCCCCCCCCCCCC
RDMEALARGLKEGIELSYLEYRIGQVEYLGEQLREAGIPIQYPTGGHAVFVDAKKMLPHI
CCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEHHHHCCCC
PYYQFPAQALGNALYLESGVRGVEIGSFLLGRDPETGEQLESPFELLRLTIPRRVYTNNH
CCCCCCHHHHCCEEEECCCCCEEEECHHEECCCCCCHHHHHHHHHHHHHHCCCCEECCCC
MDFVAQGLIKIKDKAEELKGLEFTYEPEILRHFTARLKPVEG
HHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]