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The map label for this gene is gltX1 [H]

Identifier: 220932714

GI number: 220932714

Start: 2006060

End: 2007535

Strand: Direct

Name: gltX1 [H]

Synonym: Hore_18780

Alternate gene names: 220932714

Gene position: 2006060-2007535 (Clockwise)

Preceding gene: 220932677

Following gene: 220932747

Centisome position: 77.81

GC content: 42.62

Gene sequence:

>1476_bases
ATGCCAGATAAAGTTAGAGTGAGGTTTGCCCCCAGTCCAACTGGAGATTTACATATAGGTGGGGTGAGAACAGCATTATT
TAACTGGCTTTTTGCCCGTCACAATAACGGTGACTTTATTTTAAGGATAGAAGATACCGATGCCCAAAGATCTACAGAAG
AGTCATCAAGACAGATAATAAAGGCCCTCAAGTGGGTTGGACTGGAATGGGATGAAGGCCCTGGAGTCGGAGGTGACTAT
GGCCCCTATTTTCAATCTGAACGACAACAATTTTATCAAAAAGCCATAGATTACCTTTTAGAAAATGACAAAGCCTATTA
CTGTTACTGTACCCCGGAAGAAATAGAAGACATGAAAAAAGAAGCGGATAAACGGGGAGAACCAGCTATTTATTCAGGAA
AATGCTGCAATCTGACTGAAGAAGAAAGAAAGGAACTTGAAAAAGAGGGTCGCAAGCCAGTAGTCCGCCTTAAAACACCG
GATGAAGGGTATACTGTAGTCAAAGACATTGTCAGAGGTAAAGTTTCTTTTGAAAACAGATTGTTAGAAGATTTAATAAT
CATGAAGTCTGACGGAAAACCTACCTATAATTTTGCCTGTGTTGTTGACGACCACAGGATGGGAATTACCCATGTCATCA
GGGCTGATGAGCACCTCTCCAATACCCCGAAACAGATAATGGTCTATAAAGCACTGGGTTACGATATTCCCAATTTTGCT
CATGTTCCCATGATCCTGGCCCCGGACCGGACAAAACTCAGTAAACGCCATGGAGCCACTTCAGTGGACGAATTTATGGA
ACAGGGTTATCTACCACAGGCATTAATTAATTACCTGTTATTACTCGGCTGGTCTCCGGGTAACAATGATGAAATTATCT
CCCTCGACAGGGCTATCCGTGATTTTAAACTGGACGATGTATCCAAAAACCCGGCCGTATATGATACCAAGAAGCTGACC
TGGGTTAATGGTCAGTATATGAGGAGTTTGCCACTGGAAGAAATTATTAAACATGCCCTCCCTCTTATCAAAGAGGCTGG
CTTTGTTAACTTTCCTTTAAACACCGATGAGGAAGAAAGGCTTTATAAAATAATCGATGCTGTCCGGGAAAGGAGCCGGA
CCCTGGTCGAACTTGTTGATGGAATCAGTTATTTTTACGATGATAACTTTGAATATGATGAAAAGGGTGTCAAAAAACAC
TTCATGAAAGATGATACTATCACTATTCTCGAAAACGACATTGATTGCCTGAAAAAGGTAGAACCCTTTAACCGTGAAGC
TATTGAAGAAATATACCGGGCCCGCAGTAAAGATCTTGGGGTCAAGGCCGCCCGCATGATTCATACCACCAGGCTGGCCC
TCTCCGGAAGAACCTTTGGACCAGGGCTTTTTACCCTGGTGGAACTGGTAGGCAGGGAAGAAGCCATTAAAAGACTGGAA
CGGGCTTTAGAATATGTTAAAAACAAACTTGACTAA

Upstream 100 bases:

>100_bases
TATAAATTATGTCGTTTAATTATACATTAATGTATTTTCTTGTGTTAGGTTAACCATTTTGATATAATAGCTTTCAGAAT
AACTAAGGAGGTTGTTTCCC

Downstream 100 bases:

>100_bases
ATACCCGTACCATCCAGTAAGTAGCGATTCACCGAATGATTAACGGTGCTTAGAATATGTTGCCAGCTAGCGGTTGGCGT
TACTAACCCCCCGTTCTGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MPDKVRVRFAPSPTGDLHIGGVRTALFNWLFARHNNGDFILRIEDTDAQRSTEESSRQIIKALKWVGLEWDEGPGVGGDY
GPYFQSERQQFYQKAIDYLLENDKAYYCYCTPEEIEDMKKEADKRGEPAIYSGKCCNLTEEERKELEKEGRKPVVRLKTP
DEGYTVVKDIVRGKVSFENRLLEDLIIMKSDGKPTYNFACVVDDHRMGITHVIRADEHLSNTPKQIMVYKALGYDIPNFA
HVPMILAPDRTKLSKRHGATSVDEFMEQGYLPQALINYLLLLGWSPGNNDEIISLDRAIRDFKLDDVSKNPAVYDTKKLT
WVNGQYMRSLPLEEIIKHALPLIKEAGFVNFPLNTDEEERLYKIIDAVRERSRTLVELVDGISYFYDDNFEYDEKGVKKH
FMKDDTITILENDIDCLKKVEPFNREAIEEIYRARSKDLGVKAARMIHTTRLALSGRTFGPGLFTLVELVGREEAIKRLE
RALEYVKNKLD

Sequences:

>Translated_491_residues
MPDKVRVRFAPSPTGDLHIGGVRTALFNWLFARHNNGDFILRIEDTDAQRSTEESSRQIIKALKWVGLEWDEGPGVGGDY
GPYFQSERQQFYQKAIDYLLENDKAYYCYCTPEEIEDMKKEADKRGEPAIYSGKCCNLTEEERKELEKEGRKPVVRLKTP
DEGYTVVKDIVRGKVSFENRLLEDLIIMKSDGKPTYNFACVVDDHRMGITHVIRADEHLSNTPKQIMVYKALGYDIPNFA
HVPMILAPDRTKLSKRHGATSVDEFMEQGYLPQALINYLLLLGWSPGNNDEIISLDRAIRDFKLDDVSKNPAVYDTKKLT
WVNGQYMRSLPLEEIIKHALPLIKEAGFVNFPLNTDEEERLYKIIDAVRERSRTLVELVDGISYFYDDNFEYDEKGVKKH
FMKDDTITILENDIDCLKKVEPFNREAIEEIYRARSKDLGVKAARMIHTTRLALSGRTFGPGLFTLVELVGREEAIKRLE
RALEYVKNKLD
>Mature_490_residues
PDKVRVRFAPSPTGDLHIGGVRTALFNWLFARHNNGDFILRIEDTDAQRSTEESSRQIIKALKWVGLEWDEGPGVGGDYG
PYFQSERQQFYQKAIDYLLENDKAYYCYCTPEEIEDMKKEADKRGEPAIYSGKCCNLTEEERKELEKEGRKPVVRLKTPD
EGYTVVKDIVRGKVSFENRLLEDLIIMKSDGKPTYNFACVVDDHRMGITHVIRADEHLSNTPKQIMVYKALGYDIPNFAH
VPMILAPDRTKLSKRHGATSVDEFMEQGYLPQALINYLLLLGWSPGNNDEIISLDRAIRDFKLDDVSKNPAVYDTKKLTW
VNGQYMRSLPLEEIIKHALPLIKEAGFVNFPLNTDEEERLYKIIDAVRERSRTLVELVDGISYFYDDNFEYDEKGVKKHF
MKDDTITILENDIDCLKKVEPFNREAIEEIYRARSKDLGVKAARMIHTTRLALSGRTFGPGLFTLVELVGREEAIKRLER
ALEYVKNKLD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=33.6032388663968, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1788743, Length=486, Percent_Identity=40.1234567901235, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI226510925, Length=316, Percent_Identity=31.9620253164557, Blast_Score=151, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=34.639175257732, Blast_Score=267, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=34.4554455445545, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321192, Length=290, Percent_Identity=26.551724137931, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=113, Percent_Identity=35.3982300884956, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=36.0721442885772, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24649466, Length=233, Percent_Identity=28.755364806867, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56598; Mature: 56467

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDKVRVRFAPSPTGDLHIGGVRTALFNWLFARHNNGDFILRIEDTDAQRSTEESSRQII
CCCCEEEEECCCCCCCEEECHHHHHHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHH
KALKWVGLEWDEGPGVGGDYGPYFQSERQQFYQKAIDYLLENDKAYYCYCTPEEIEDMKK
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
EADKRGEPAIYSGKCCNLTEEERKELEKEGRKPVVRLKTPDEGYTVVKDIVRGKVSFENR
HHHCCCCCCEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHH
LLEDLIIMKSDGKPTYNFACVVDDHRMGITHVIRADEHLSNTPKQIMVYKALGYDIPNFA
HHHHHHEEECCCCCCEEEEEEEECCCCCHHHHHHCCHHHCCCCHHEEEEHHHCCCCCCCC
HVPMILAPDRTKLSKRHGATSVDEFMEQGYLPQALINYLLLLGWSPGNNDEIISLDRAIR
CCCEEECCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHH
DFKLDDVSKNPAVYDTKKLTWVNGQYMRSLPLEEIIKHALPLIKEAGFVNFPLNTDEEER
HCCCCCCCCCCCEEECCEEEEECCHHHHCCCHHHHHHHHHHHHHHCCEEECCCCCCHHHH
LYKIIDAVRERSRTLVELVDGISYFYDDNFEYDEKGVKKHFMKDDTITILENDIDCLKKV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHC
EPFNREAIEEIYRARSKDLGVKAARMIHTTRLALSGRTFGPGLFTLVELVGREEAIKRLE
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCHHHHHHHHH
RALEYVKNKLD
HHHHHHHHHCC
>Mature Secondary Structure 
PDKVRVRFAPSPTGDLHIGGVRTALFNWLFARHNNGDFILRIEDTDAQRSTEESSRQII
CCCEEEEECCCCCCCEEECHHHHHHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHH
KALKWVGLEWDEGPGVGGDYGPYFQSERQQFYQKAIDYLLENDKAYYCYCTPEEIEDMKK
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
EADKRGEPAIYSGKCCNLTEEERKELEKEGRKPVVRLKTPDEGYTVVKDIVRGKVSFENR
HHHCCCCCCEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHH
LLEDLIIMKSDGKPTYNFACVVDDHRMGITHVIRADEHLSNTPKQIMVYKALGYDIPNFA
HHHHHHEEECCCCCCEEEEEEEECCCCCHHHHHHCCHHHCCCCHHEEEEHHHCCCCCCCC
HVPMILAPDRTKLSKRHGATSVDEFMEQGYLPQALINYLLLLGWSPGNNDEIISLDRAIR
CCCEEECCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHH
DFKLDDVSKNPAVYDTKKLTWVNGQYMRSLPLEEIIKHALPLIKEAGFVNFPLNTDEEER
HCCCCCCCCCCCEEECCEEEEECCHHHHCCCHHHHHHHHHHHHHHCCEEECCCCCCHHHH
LYKIIDAVRERSRTLVELVDGISYFYDDNFEYDEKGVKKHFMKDDTITILENDIDCLKKV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHC
EPFNREAIEEIYRARSKDLGVKAARMIHTTRLALSGRTFGPGLFTLVELVGREEAIKRLE
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCHHHHHHHHH
RALEYVKNKLD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]