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The map label for this gene is cspR [H]

Identifier: 220931942

GI number: 220931942

Start: 1195222

End: 1195710

Strand: Direct

Name: cspR [H]

Synonym: Hore_11010

Alternate gene names: 220931942

Gene position: 1195222-1195710 (Clockwise)

Preceding gene: 220931941

Following gene: 220931943

Centisome position: 46.36

GC content: 35.17

Gene sequence:

>489_bases
TTGAATATAGTCCTCGTTGAACCTGAAATACCGCAAAATACTGGTAACATTGCCAGGACATGTGCTGTAACCGGATCAAG
TCTTCATTTAATAGAACCCCTTGGCTTTTCAATTGATGACAGGTACCTGAAAAGGGCCGGCCTTGACTACTGGGATAAAC
TTGATATATATTATTATGATTCCCTGGAAGAGCTAATGAAAAAATATAAAGATTCCAATTTTTATTTTGCTACTACCAAA
GCACCAAAGTCATATGATATTGTTTCATATCAGGAGAATGATTTCATTGTTTTTGGTAAGGAAACAGCCGGTCTGCCGGA
ATCTTTACTTAAGAATAACATTGAGAATTGTATTAGAATTCCCATGAAGGACAATACCAGGTCTTTAAATCTATCAAATT
CGGTTGCCATTATTCTCTATGAGGCATTAAGACAGCTTAATTTTCCAGGATTAAAACATGAGGGTAAATTTATAAATATA
GGCAAGTAG

Upstream 100 bases:

>100_bases
ATAGATCAGGGAATGGTATTATATCAATCTATTATTAAAAAATTAATTATTATAATATTTGTTTATATAATAAATTTAAC
CATGAAAGGAGGATAAAACT

Downstream 100 bases:

>100_bases
TATGGAATAAATTACAAATAAATGGATGCAGGAATTTATTAATATACTGTCGAATATAATAGATACCTATGTTTATTCTA
AATGGTATATATAACCAGTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MNIVLVEPEIPQNTGNIARTCAVTGSSLHLIEPLGFSIDDRYLKRAGLDYWDKLDIYYYDSLEELMKKYKDSNFYFATTK
APKSYDIVSYQENDFIVFGKETAGLPESLLKNNIENCIRIPMKDNTRSLNLSNSVAIILYEALRQLNFPGLKHEGKFINI
GK

Sequences:

>Translated_162_residues
MNIVLVEPEIPQNTGNIARTCAVTGSSLHLIEPLGFSIDDRYLKRAGLDYWDKLDIYYYDSLEELMKKYKDSNFYFATTK
APKSYDIVSYQENDFIVFGKETAGLPESLLKNNIENCIRIPMKDNTRSLNLSNSVAIILYEALRQLNFPGLKHEGKFINI
GK
>Mature_162_residues
MNIVLVEPEIPQNTGNIARTCAVTGSSLHLIEPLGFSIDDRYLKRAGLDYWDKLDIYYYDSLEELMKKYKDSNFYFATTK
APKSYDIVSYQENDFIVFGKETAGLPESLLKNNIENCIRIPMKDNTRSLNLSNSVAIILYEALRQLNFPGLKHEGKFINI
GK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=48.3443708609272, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18539; Mature: 18539

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVLVEPEIPQNTGNIARTCAVTGSSLHLIEPLGFSIDDRYLKRAGLDYWDKLDIYYYD
CEEEEECCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCCHHCEEEEEEC
SLEELMKKYKDSNFYFATTKAPKSYDIVSYQENDFIVFGKETAGLPESLLKNNIENCIRI
CHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHCCHHHEEEC
PMKDNTRSLNLSNSVAIILYEALRQLNFPGLKHEGKFINIGK
CCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MNIVLVEPEIPQNTGNIARTCAVTGSSLHLIEPLGFSIDDRYLKRAGLDYWDKLDIYYYD
CEEEEECCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHCCCCCHHCEEEEEEC
SLEELMKKYKDSNFYFATTKAPKSYDIVSYQENDFIVFGKETAGLPESLLKNNIENCIRI
CHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHCCHHHEEEC
PMKDNTRSLNLSNSVAIILYEALRQLNFPGLKHEGKFINIGK
CCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]