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The map label for this gene is ispH [H]

Identifier: 220931888

GI number: 220931888

Start: 1134659

End: 1136695

Strand: Direct

Name: ispH [H]

Synonym: Hore_10450

Alternate gene names: 220931888

Gene position: 1134659-1136695 (Clockwise)

Preceding gene: 220931887

Following gene: 220931889

Centisome position: 44.01

GC content: 38.0

Gene sequence:

>2037_bases
ATGGAAGCTGCCGGGAATAATAAAGAAGAAATCCCTGTATATACCCTGGGCCCTTTAATTCATAATCCACAGGTTGTAAA
AAGATTAAAAGAGATGGGTATTGAATCTTTAGATTCACTTGATATAATTGATAATGGGGTTATCATTATACGGTCACATG
GTGTACCCCCTGATATAATAGAGAAAGCCCGTCAAAAGGGTTTACATATAATAGACGCAACCTGTCCCTATGTAAAAAAT
GCCCAGAAGTATGCCAGAGAGCTTGTTGATAAAGGATACCAGACAATAATTTTTGGGGATAGAGACCACCCGGAAGTTAT
CGGTATCAACGGGGCAGCGCGTAATGAGTCCATTATTATAAGCAGTGAGAAGGAAATAAAAGATATTAACCTTTCCAGTA
AAGTTGGGCTAATAGCCCAGACTACCAAATCACCTGCTTCTTTTCAAAAGATTGTAAGTTATATTTTACCTGAAGTTAAA
GAGTTACGGATTTACAATACTATCTGTAGAACTACAGAAACAAGGCAAAAATCTGCCGTTGATTTAGCCAGACAGGTAGA
TGCTATGTTCGTAATTGGGGGAAAAAACAGTGCTAATACTACCCGGTTAGCAGAACTGTGTGCTGAAACTGGGACCCCAA
CTTATCATATAGAGACTGCCGGAGAGATTGATTTCAGGCAGTTGAAATCTTACAATAAAATAGGTATTACAGCAGGGGCT
TCGACCCCAGACTGGTTAATTAAGGAGGTTATTGAGGCTATGAGTGAAGAAAAAAGAGATGCAGAATTACAGGAAGAAAT
TCAAGAGGGAGTAAATACTGATAATACTCCTGAAACACCAAATGGTGAAGAAAATCCCATTGAAAATGAGGAAGAATTTA
ACTACGATGACAATAATCCCGCTGATTTAAAAAAAGGTCAGCAGGTAAAAGGTAAAGTTGTAGAAATTGGGGATAATGGG
GTTTATGTGGATGTCGGATATAAAACCGAAGGTTTAATACCCCTCAGGGAATTAAGCCACCGGGATTTTGAAGACCCCCA
TGAAATTGTCGAAGAAGGTGAAGAAATCGATGTAATCGTTCTTACCCTTGAAGATGAAGAAGGAAATATGGTCTTATCAA
AAAAACGGGCTGATTTTGAGAAGGCCTGGGAGCGTATCCTGAAAGCCTATGAAAATGATGAAATCATCGAAGCAGAAGTT
ACCAGAGAAGTTAAAGGTGGTCTTGTAGTAGATATTGGTCTCCGTGGTTTTGTACATGCTTCCCATGTGGCAATTGGATA
TGTTGAAGATTTAAGTGATTATGTTGGGAAAACATTACGTTTAAAAGTTATAGAAGTTGAACGTGATAAAAACAATGTTG
TTCTATCTGCTAAAAAAGTTCTGGAAGAAGAGAGAGAAAAGCAGAAAGAGAAAACCCTGGAAGAATTACAAGAAGGACAG
GTTGTTAAAGGTAAAGTTACCAAGATAGTAGATTTTGGTGCTTTCGTGGATCTGGGAGGAATTGAAGGCCTCTTACACAT
TTCAGAAATGTCCTGGGGTAGGATAGAACATCCTTCTGAAGTATTGGAAGAAGGTCAGGAAATAGAGGTTAAAGTACTGG
CAGTAAACAAAGAAGAGGAAAGAATCTCACTTGGACTAAAACAGCTTTTACCTGATCCCTGGGAAGAGTTTGCTAAAAAG
CATTATGAAGGAGAGGTAGTTTCTGGTACAATTACTAAAATAGTAGACTTTGGAGCTTTCATGGAAATTGAAAATGGTAT
AGAGGGATTAATTCATATTTCACAGCTATCACATCGTCATGTAAAAACTCCAGATGAAGTAGTATCTGTAGGAGAAGAAA
TCAAGGCAGAGATTATTAATATTGACCCAGAGCAGAAAAGGGTTGGATTGAGTATAAAAGCACTTGAAGAACAACCAGAA
ACACCAAAAAAAGTCGAAGCACCAAAAAAGGAACCTACCAGGGAAAATGAATCTACCTCTGGAGTAACCATTGGAGAGAT
TGTTGGGGATATTCTTAAAAAAGAAGATGATAAATAA

Upstream 100 bases:

>100_bases
ATAGCTGGTGCTGGGGTTGAAGGGGGTGTCGGTTCTGGAGGTATATGTCGCAGATGAATCTGGTTTTTGTTTTGGAGTTA
AACGAGCAATTAATCTAGCA

Downstream 100 bases:

>100_bases
TCACAGTTTAATATAATTTTTGCTGTTTTTAGATCATAACCGCCTCCGGGCGGTATTTTTTTTATGTATATACAACCTGA
TTGTGGGAAACATATTTTAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MEAAGNNKEEIPVYTLGPLIHNPQVVKRLKEMGIESLDSLDIIDNGVIIIRSHGVPPDIIEKARQKGLHIIDATCPYVKN
AQKYARELVDKGYQTIIFGDRDHPEVIGINGAARNESIIISSEKEIKDINLSSKVGLIAQTTKSPASFQKIVSYILPEVK
ELRIYNTICRTTETRQKSAVDLARQVDAMFVIGGKNSANTTRLAELCAETGTPTYHIETAGEIDFRQLKSYNKIGITAGA
STPDWLIKEVIEAMSEEKRDAELQEEIQEGVNTDNTPETPNGEENPIENEEEFNYDDNNPADLKKGQQVKGKVVEIGDNG
VYVDVGYKTEGLIPLRELSHRDFEDPHEIVEEGEEIDVIVLTLEDEEGNMVLSKKRADFEKAWERILKAYENDEIIEAEV
TREVKGGLVVDIGLRGFVHASHVAIGYVEDLSDYVGKTLRLKVIEVERDKNNVVLSAKKVLEEEREKQKEKTLEELQEGQ
VVKGKVTKIVDFGAFVDLGGIEGLLHISEMSWGRIEHPSEVLEEGQEIEVKVLAVNKEEERISLGLKQLLPDPWEEFAKK
HYEGEVVSGTITKIVDFGAFMEIENGIEGLIHISQLSHRHVKTPDEVVSVGEEIKAEIINIDPEQKRVGLSIKALEEQPE
TPKKVEAPKKEPTRENESTSGVTIGEIVGDILKKEDDK

Sequences:

>Translated_678_residues
MEAAGNNKEEIPVYTLGPLIHNPQVVKRLKEMGIESLDSLDIIDNGVIIIRSHGVPPDIIEKARQKGLHIIDATCPYVKN
AQKYARELVDKGYQTIIFGDRDHPEVIGINGAARNESIIISSEKEIKDINLSSKVGLIAQTTKSPASFQKIVSYILPEVK
ELRIYNTICRTTETRQKSAVDLARQVDAMFVIGGKNSANTTRLAELCAETGTPTYHIETAGEIDFRQLKSYNKIGITAGA
STPDWLIKEVIEAMSEEKRDAELQEEIQEGVNTDNTPETPNGEENPIENEEEFNYDDNNPADLKKGQQVKGKVVEIGDNG
VYVDVGYKTEGLIPLRELSHRDFEDPHEIVEEGEEIDVIVLTLEDEEGNMVLSKKRADFEKAWERILKAYENDEIIEAEV
TREVKGGLVVDIGLRGFVHASHVAIGYVEDLSDYVGKTLRLKVIEVERDKNNVVLSAKKVLEEEREKQKEKTLEELQEGQ
VVKGKVTKIVDFGAFVDLGGIEGLLHISEMSWGRIEHPSEVLEEGQEIEVKVLAVNKEEERISLGLKQLLPDPWEEFAKK
HYEGEVVSGTITKIVDFGAFMEIENGIEGLIHISQLSHRHVKTPDEVVSVGEEIKAEIINIDPEQKRVGLSIKALEEQPE
TPKKVEAPKKEPTRENESTSGVTIGEIVGDILKKEDDK
>Mature_678_residues
MEAAGNNKEEIPVYTLGPLIHNPQVVKRLKEMGIESLDSLDIIDNGVIIIRSHGVPPDIIEKARQKGLHIIDATCPYVKN
AQKYARELVDKGYQTIIFGDRDHPEVIGINGAARNESIIISSEKEIKDINLSSKVGLIAQTTKSPASFQKIVSYILPEVK
ELRIYNTICRTTETRQKSAVDLARQVDAMFVIGGKNSANTTRLAELCAETGTPTYHIETAGEIDFRQLKSYNKIGITAGA
STPDWLIKEVIEAMSEEKRDAELQEEIQEGVNTDNTPETPNGEENPIENEEEFNYDDNNPADLKKGQQVKGKVVEIGDNG
VYVDVGYKTEGLIPLRELSHRDFEDPHEIVEEGEEIDVIVLTLEDEEGNMVLSKKRADFEKAWERILKAYENDEIIEAEV
TREVKGGLVVDIGLRGFVHASHVAIGYVEDLSDYVGKTLRLKVIEVERDKNNVVLSAKKVLEEEREKQKEKTLEELQEGQ
VVKGKVTKIVDFGAFVDLGGIEGLLHISEMSWGRIEHPSEVLEEGQEIEVKVLAVNKEEERISLGLKQLLPDPWEEFAKK
HYEGEVVSGTITKIVDFGAFMEIENGIEGLIHISQLSHRHVKTPDEVVSVGEEIKAEIINIDPEQKRVGLSIKALEEQPE
TPKKVEAPKKEPTRENESTSGVTIGEIVGDILKKEDDK

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0539

COG function: function code J; Ribosomal protein S1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 S1 motif domains [H]

Homologues:

Organism=Homo sapiens, GI70980549, Length=270, Percent_Identity=28.5185185185185, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1787140, Length=338, Percent_Identity=40.8284023668639, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1786212, Length=254, Percent_Identity=29.1338582677165, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI145693187, Length=83, Percent_Identity=46.9879518072289, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082262, Length=99, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323885, Length=347, Percent_Identity=23.6311239193084, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: 16,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR000110
- InterPro:   IPR022967 [H]

Pfam domain/function: PF02401 LYTB; PF00575 S1 [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 75737; Mature: 75737

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAAGNNKEEIPVYTLGPLIHNPQVVKRLKEMGIESLDSLDIIDNGVIIIRSHGVPPDII
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCHHHH
EKARQKGLHIIDATCPYVKNAQKYARELVDKGYQTIIFGDRDHPEVIGINGAARNESIII
HHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCEEEE
SSEKEIKDINLSSKVGLIAQTTKSPASFQKIVSYILPEVKELRIYNTICRTTETRQKSAV
ECCCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHH
DLARQVDAMFVIGGKNSANTTRLAELCAETGTPTYHIETAGEIDFRQLKSYNKIGITAGA
HHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEEECC
STPDWLIKEVIEAMSEEKRDAELQEEIQEGVNTDNTPETPNGEENPIENEEEFNYDDNNP
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADLKKGQQVKGKVVEIGDNGVYVDVGYKTEGLIPLRELSHRDFEDPHEIVEEGEEIDVIV
HHHCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHCCCCEEEEE
LTLEDEEGNMVLSKKRADFEKAWERILKAYENDEIIEAEVTREVKGGLVVDIGLRGFVHA
EEEECCCCCEEEEHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCEEEEECCCCCEEH
SHVAIGYVEDLSDYVGKTLRLKVIEVERDKNNVVLSAKKVLEEEREKQKEKTLEELQEGQ
HHHEEHHHHHHHHHCCCEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC
VVKGKVTKIVDFGAFVDLGGIEGLLHISEMSWGRIEHPSEVLEEGQEIEVKVLAVNKEEE
EEECHHHHHEECCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCEEEEEEEECCCHH
RISLGLKQLLPDPWEEFAKKHYEGEVVSGTITKIVDFGAFMEIENGIEGLIHISQLSHRH
HHHHHHHHHCCCHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCHHHHEEHHHHHCCC
VKTPDEVVSVGEEIKAEIINIDPEQKRVGLSIKALEEQPETPKKVEAPKKEPTRENESTS
CCCHHHHHHHHHHHHHEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GVTIGEIVGDILKKEDDK
CCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEAAGNNKEEIPVYTLGPLIHNPQVVKRLKEMGIESLDSLDIIDNGVIIIRSHGVPPDII
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCHHHH
EKARQKGLHIIDATCPYVKNAQKYARELVDKGYQTIIFGDRDHPEVIGINGAARNESIII
HHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCEEEE
SSEKEIKDINLSSKVGLIAQTTKSPASFQKIVSYILPEVKELRIYNTICRTTETRQKSAV
ECCCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHH
DLARQVDAMFVIGGKNSANTTRLAELCAETGTPTYHIETAGEIDFRQLKSYNKIGITAGA
HHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEEECC
STPDWLIKEVIEAMSEEKRDAELQEEIQEGVNTDNTPETPNGEENPIENEEEFNYDDNNP
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADLKKGQQVKGKVVEIGDNGVYVDVGYKTEGLIPLRELSHRDFEDPHEIVEEGEEIDVIV
HHHCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHCCCCEEEEE
LTLEDEEGNMVLSKKRADFEKAWERILKAYENDEIIEAEVTREVKGGLVVDIGLRGFVHA
EEEECCCCCEEEEHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCCEEEEECCCCCEEH
SHVAIGYVEDLSDYVGKTLRLKVIEVERDKNNVVLSAKKVLEEEREKQKEKTLEELQEGQ
HHHEEHHHHHHHHHCCCEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC
VVKGKVTKIVDFGAFVDLGGIEGLLHISEMSWGRIEHPSEVLEEGQEIEVKVLAVNKEEE
EEECHHHHHEECCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCEEEEEEEECCCHH
RISLGLKQLLPDPWEEFAKKHYEGEVVSGTITKIVDFGAFMEIENGIEGLIHISQLSHRH
HHHHHHHHHCCCHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCHHHHEEHHHHHCCC
VKTPDEVVSVGEEIKAEIINIDPEQKRVGLSIKALEEQPETPKKVEAPKKEPTRENESTS
CCCHHHHHHHHHHHHHEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GVTIGEIVGDILKKEDDK
CCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]