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The map label for this gene is mjaIIIM [H]

Identifier: 220931288

GI number: 220931288

Start: 475626

End: 476546

Strand: Reverse

Name: mjaIIIM [H]

Synonym: Hore_04430

Alternate gene names: 220931288

Gene position: 476546-475626 (Counterclockwise)

Preceding gene: 220931293

Following gene: 220931260

Centisome position: 18.48

GC content: 29.32

Gene sequence:

>921_bases
ATGAACTTACCATGTGAGGCAAATACAACCCGGAAAACAACAAAATATGCAAAGCCATTTGTAAAATGGGCCGGAGGAAA
AAGACAACTTATTCCTGATTTATTTAATTCATTACCTGATAATATTGAAAGATACTTTGAGCCCTTTGTAGGTGCCGGAG
CTCTTTTTTTTGAATTAGCAAATAACAATGTAATATCCGGAAGAAAAAGTATTATAGTAGACCTTAATGATGATCTGATA
AATGCATATAAAGTTATAAAAAATTCTGTTGAATCTCTTATTAATGAATTAAAAAAACATGAAAAAAATAATAATGAAAT
ATATTATTATAAAGTTAGAGGACTATGGACGCGAAAGGCCAGAGAGTTAGGTCTATCCAGGAAAGAACTATGGAGTGTAA
ATGAGCTTAACAATATCCAGAAAGCAGCCAGATTTATATATCTTAATAAAACCTGCTACAATGGTTTATACCGTTTGAAT
AATAAAGGTCAATTTAATGTACCTTATGGTAATTATAAAAATCCCAGGATATGTGATGAAGATAATCTAAGGACTGTTAG
CCAATTATTACAAAACGTTACAATAATTAAAAACGATTTTGAAATAATAAAAGATAAGTATAGATTTACACCGGCCGATT
TTGTTTACTTTGATCCTCCCTATGTACCCTTGTCAGATACTTCTAATTTTACTTCTTATACAAAAAATGGGTTTACTAAA
GAAGATCAGGAAAGGTTATACGAATTATTCAATTATATCGATATGCAGGGTGGGAAATGCATGTTAAGCAATTCAAACTC
TCCATTGATAAAAAAACTATATTCAAAATTCAATATTAAAGAAGTTAAAGCAATTAGAGCTATAAATTGTATTGGAAACA
AACGGGGAGCAATAAAAGAATTAATAATAACTAACTATTAA

Upstream 100 bases:

>100_bases
ATTGTTCTGATTTTTTGAGTAAGTTTTGAAGGATATTATAATATTATATTGAATATAACTTATAAGGTTATTTTGGATGA
TACTCAGGAGGTATATAATA

Downstream 100 bases:

>100_bases
TTAAAGCAAGGGTATATGCCCTTGCTTTTTTAAATTTTTATGATTTTATTTTTTTCTGTGTAAGGTTAATAATCCATTCA
AGCCATTTTCTAACTGTTTG

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNLPCEANTTRKTTKYAKPFVKWAGGKRQLIPDLFNSLPDNIERYFEPFVGAGALFFELANNNVISGRKSIIVDLNDDLI
NAYKVIKNSVESLINELKKHEKNNNEIYYYKVRGLWTRKARELGLSRKELWSVNELNNIQKAARFIYLNKTCYNGLYRLN
NKGQFNVPYGNYKNPRICDEDNLRTVSQLLQNVTIIKNDFEIIKDKYRFTPADFVYFDPPYVPLSDTSNFTSYTKNGFTK
EDQERLYELFNYIDMQGGKCMLSNSNSPLIKKLYSKFNIKEVKAIRAINCIGNKRGAIKELIITNY

Sequences:

>Translated_306_residues
MNLPCEANTTRKTTKYAKPFVKWAGGKRQLIPDLFNSLPDNIERYFEPFVGAGALFFELANNNVISGRKSIIVDLNDDLI
NAYKVIKNSVESLINELKKHEKNNNEIYYYKVRGLWTRKARELGLSRKELWSVNELNNIQKAARFIYLNKTCYNGLYRLN
NKGQFNVPYGNYKNPRICDEDNLRTVSQLLQNVTIIKNDFEIIKDKYRFTPADFVYFDPPYVPLSDTSNFTSYTKNGFTK
EDQERLYELFNYIDMQGGKCMLSNSNSPLIKKLYSKFNIKEVKAIRAINCIGNKRGAIKELIITNY
>Mature_306_residues
MNLPCEANTTRKTTKYAKPFVKWAGGKRQLIPDLFNSLPDNIERYFEPFVGAGALFFELANNNVISGRKSIIVDLNDDLI
NAYKVIKNSVESLINELKKHEKNNNEIYYYKVRGLWTRKARELGLSRKELWSVNELNNIQKAARFIYLNKTCYNGLYRLN
NKGQFNVPYGNYKNPRICDEDNLRTVSQLLQNVTIIKNDFEIIKDKYRFTPADFVYFDPPYVPLSDTSNFTSYTKNGFTK
EDQERLYELFNYIDMQGGKCMLSNSNSPLIKKLYSKFNIKEVKAIRAINCIGNKRGAIKELIITNY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=292, Percent_Identity=34.5890410958904, Blast_Score=141, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 35614; Mature: 35614

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPCEANTTRKTTKYAKPFVKWAGGKRQLIPDLFNSLPDNIERYFEPFVGAGALFFELA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEC
NNNVISGRKSIIVDLNDDLINAYKVIKNSVESLINELKKHEKNNNEIYYYKVRGLWTRKA
CCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHH
RELGLSRKELWSVNELNNIQKAARFIYLNKTCYNGLYRLNNKGQFNVPYGNYKNPRICDE
HHHCCCHHHHHCHHHHHHHHHHHHHEEECHHHHCCCEEECCCCEEECCCCCCCCCCCCCC
DNLRTVSQLLQNVTIIKNDFEIIKDKYRFTPADFVYFDPPYVPLSDTSNFTSYTKNGFTK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCC
EDQERLYELFNYIDMQGGKCMLSNSNSPLIKKLYSKFNIKEVKAIRAINCIGNKRGAIKE
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
LIITNY
HHHCCC
>Mature Secondary Structure
MNLPCEANTTRKTTKYAKPFVKWAGGKRQLIPDLFNSLPDNIERYFEPFVGAGALFFELA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEC
NNNVISGRKSIIVDLNDDLINAYKVIKNSVESLINELKKHEKNNNEIYYYKVRGLWTRKA
CCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHH
RELGLSRKELWSVNELNNIQKAARFIYLNKTCYNGLYRLNNKGQFNVPYGNYKNPRICDE
HHHCCCHHHHHCHHHHHHHHHHHHHEEECHHHHCCCEEECCCCEEECCCCCCCCCCCCCC
DNLRTVSQLLQNVTIIKNDFEIIKDKYRFTPADFVYFDPPYVPLSDTSNFTSYTKNGFTK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCC
EDQERLYELFNYIDMQGGKCMLSNSNSPLIKKLYSKFNIKEVKAIRAINCIGNKRGAIKE
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
LIITNY
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]