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The map label for this gene is icaA [H]

Identifier: 220918766

GI number: 220918766

Start: 4095412

End: 4096851

Strand: Direct

Name: icaA [H]

Synonym: A2cp1_3677

Alternate gene names: 220918766

Gene position: 4095412-4096851 (Clockwise)

Preceding gene: 220918765

Following gene: 220918767

Centisome position: 81.43

GC content: 72.29

Gene sequence:

>1440_bases
GTGACGCTGCTCCGGCTCATCGAGGTCGTGCAGTGGGCGTTCCTCGCCTACTTCGTCGCGCTCACCGTGTCGTACCTCGC
GCTCAACCTCGTCTCGCTGCGGGTCATCGCGCGCGACGCGGACGCGCGCGCCTCGCTCCTCCTGCCCCGGTTCTTCTCCC
GGCTCGAGCCGCCGGTGAGCATCATCGTCCCGGCGTTCAACGAGGAGGAGACCATCGCCGGCACCATCCGGTGCCTGCTC
CAGCTCGAGTACCCCGAGCTCGAGATCGTGGTGGTGAACGACGGGTCCACCGACCGCACGCTCGAGGTCCTCACCCGCGA
GTTCGAGCTGGTGCCGTTCCCCGAGGCGTACCGCATCTCGGTGCCCTCCACGCCGGTCCGGCGGGTGTTCCGGTCGCTCC
GCCACCGCAACGTGCGCGTGCTCGACAAGTCGAACGGCGGCAAGGCGGACGCCATGAACGCGGGCATCAACGCCGCCCGG
TACCCGCTGTTCTGCGCGGTGGACGGCGACTCGGTGCTGCAGCGCGACAGCGTGGCGCGGGTGGTCCGGCCGTTCCTGGA
GGACCCTTCCACCATCGCCTGCGGCGGCACCATCCGCGTGCTGAACGGCTGCAGGGTGGTGGACGGGTTCGTCGAGGAGA
TCGGGATGCCGCGCGGCTGGGTCGCCCGGGTGCAGGTGCTCGAGTACCTGCGGGCGTTCCTGTTCGGCCGGCTGGGCTGG
GCGCCGGTGAACGCGCTCCCCAACGTCTCCGGCGCGTTCGGGATGTTCCGCCGCACCGCCGTCATCGAGGCCGGCGGCTA
CCGGACCGACACCGTCGGCGAGGACATGGAGCTCGTGCTGCGGCTGCACCGCCTCTACCGGCTCTCCGGCCGCCCCTACC
GCATCTCGTTCGTGCCCGACCCGATCTGCTGGACCGAGGTGCCGGACACGCTCGCCGCGCTCCGCAGGCAGCGCGTCCGC
TGGCACCGCGGCCTGCTCGAGGCCATCGCCGGCAACCGCGGGCTCCTGTTCCACCGCCGCGGCGGCGCCGCCGGCTGGCT
CACCGTGCCGAACCTGCTCTTCTTCGAGGCGTTCGGTCCCGTCGTCGAGGTGGCCGGGTACGTGGTGATCGGCGCCGCCT
GGGCGCTCGGCGTCATCTCCTGGAGCTCGTTCGCGGCGTTCTTCGTCGCCGCGCTCGGGGTCGCGACGCTGCTCTCCGCG
AACGCGCTGCTCCTCGAGGAGAAGTCGTTCCACGTGTACCAGCGCCCGAAGCAGCTCGCGGCGCTCATCGGCGCGGTGCT
GGTCGAGAACCTCGGCCTCCGCCAGCTCACCGCCTTCTGGCGGATCTCCGGGATGCTGAGCTGGCTGTTCCGCCGGCCCA
TCGCCTGGGGCTCGCCCGCGACCGCCGCCGCGGCAGACGCCGAGGCGCGCGACGACGAGGACGCCGGGCGGGCGGCGTAG

Upstream 100 bases:

>100_bases
GGCCGTACGGCACCTCGCAGGAGGTGCTCGCGCTGGCCGAGGCGTCCGGCGACCGCTACGCGCGCGACATCGTCCGGCAG
GCGCTCGCGGAGGTGCGGTC

Downstream 100 bases:

>100_bases
CCGCGTCCGCGGGTGGGCCGGGGCGGCTCGCCCCACCGTGAACCCCGGCCGCCGCCCCGCGTCACCGGACGTGCGCCTCG
CCGGCTTGCATTTTGGGTGG

Product: family 2 glycosyl transferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLLRLIEVVQWAFLAYFVALTVSYLALNLVSLRVIARDADARASLLLPRFFSRLEPPVSIIVPAFNEEETIAGTIRCLL
QLEYPELEIVVVNDGSTDRTLEVLTREFELVPFPEAYRISVPSTPVRRVFRSLRHRNVRVLDKSNGGKADAMNAGINAAR
YPLFCAVDGDSVLQRDSVARVVRPFLEDPSTIACGGTIRVLNGCRVVDGFVEEIGMPRGWVARVQVLEYLRAFLFGRLGW
APVNALPNVSGAFGMFRRTAVIEAGGYRTDTVGEDMELVLRLHRLYRLSGRPYRISFVPDPICWTEVPDTLAALRRQRVR
WHRGLLEAIAGNRGLLFHRRGGAAGWLTVPNLLFFEAFGPVVEVAGYVVIGAAWALGVISWSSFAAFFVAALGVATLLSA
NALLLEEKSFHVYQRPKQLAALIGAVLVENLGLRQLTAFWRISGMLSWLFRRPIAWGSPATAAAADAEARDDEDAGRAA

Sequences:

>Translated_479_residues
MTLLRLIEVVQWAFLAYFVALTVSYLALNLVSLRVIARDADARASLLLPRFFSRLEPPVSIIVPAFNEEETIAGTIRCLL
QLEYPELEIVVVNDGSTDRTLEVLTREFELVPFPEAYRISVPSTPVRRVFRSLRHRNVRVLDKSNGGKADAMNAGINAAR
YPLFCAVDGDSVLQRDSVARVVRPFLEDPSTIACGGTIRVLNGCRVVDGFVEEIGMPRGWVARVQVLEYLRAFLFGRLGW
APVNALPNVSGAFGMFRRTAVIEAGGYRTDTVGEDMELVLRLHRLYRLSGRPYRISFVPDPICWTEVPDTLAALRRQRVR
WHRGLLEAIAGNRGLLFHRRGGAAGWLTVPNLLFFEAFGPVVEVAGYVVIGAAWALGVISWSSFAAFFVAALGVATLLSA
NALLLEEKSFHVYQRPKQLAALIGAVLVENLGLRQLTAFWRISGMLSWLFRRPIAWGSPATAAAADAEARDDEDAGRAA
>Mature_478_residues
TLLRLIEVVQWAFLAYFVALTVSYLALNLVSLRVIARDADARASLLLPRFFSRLEPPVSIIVPAFNEEETIAGTIRCLLQ
LEYPELEIVVVNDGSTDRTLEVLTREFELVPFPEAYRISVPSTPVRRVFRSLRHRNVRVLDKSNGGKADAMNAGINAARY
PLFCAVDGDSVLQRDSVARVVRPFLEDPSTIACGGTIRVLNGCRVVDGFVEEIGMPRGWVARVQVLEYLRAFLFGRLGWA
PVNALPNVSGAFGMFRRTAVIEAGGYRTDTVGEDMELVLRLHRLYRLSGRPYRISFVPDPICWTEVPDTLAALRRQRVRW
HRGLLEAIAGNRGLLFHRRGGAAGWLTVPNLLFFEAFGPVVEVAGYVVIGAAWALGVISWSSFAAFFVAALGVATLLSAN
ALLLEEKSFHVYQRPKQLAALIGAVLVENLGLRQLTAFWRISGMLSWLFRRPIAWGSPATAAAADAEARDDEDAGRAA

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=287, Percent_Identity=28.9198606271777, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 52979; Mature: 52848

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLRLIEVVQWAFLAYFVALTVSYLALNLVSLRVIARDADARASLLLPRFFSRLEPPVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
IIVPAFNEEETIAGTIRCLLQLEYPELEIVVVNDGSTDRTLEVLTREFELVPFPEAYRIS
EEEECCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
VPSTPVRRVFRSLRHRNVRVLDKSNGGKADAMNAGINAARYPLFCAVDGDSVLQRDSVAR
CCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHH
VVRPFLEDPSTIACGGTIRVLNGCRVVDGFVEEIGMPRGWVARVQVLEYLRAFLFGRLGW
HHHHHHCCCCEEECCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
APVNALPNVSGAFGMFRRTAVIEAGGYRTDTVGEDMELVLRLHRLYRLSGRPYRISFVPD
CCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
PICWTEVPDTLAALRRQRVRWHRGLLEAIAGNRGLLFHRRGGAAGWLTVPNLLFFEAFGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
VVEVAGYVVIGAAWALGVISWSSFAAFFVAALGVATLLSANALLLEEKSFHVYQRPKQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHCHHHHH
ALIGAVLVENLGLRQLTAFWRISGMLSWLFRRPIAWGSPATAAAADAEARDDEDAGRAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLLRLIEVVQWAFLAYFVALTVSYLALNLVSLRVIARDADARASLLLPRFFSRLEPPVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
IIVPAFNEEETIAGTIRCLLQLEYPELEIVVVNDGSTDRTLEVLTREFELVPFPEAYRIS
EEEECCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
VPSTPVRRVFRSLRHRNVRVLDKSNGGKADAMNAGINAARYPLFCAVDGDSVLQRDSVAR
CCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHH
VVRPFLEDPSTIACGGTIRVLNGCRVVDGFVEEIGMPRGWVARVQVLEYLRAFLFGRLGW
HHHHHHCCCCEEECCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
APVNALPNVSGAFGMFRRTAVIEAGGYRTDTVGEDMELVLRLHRLYRLSGRPYRISFVPD
CCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
PICWTEVPDTLAALRRQRVRWHRGLLEAIAGNRGLLFHRRGGAAGWLTVPNLLFFEAFGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
VVEVAGYVVIGAAWALGVISWSSFAAFFVAALGVATLLSANALLLEEKSFHVYQRPKQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHCHHHHH
ALIGAVLVENLGLRQLTAFWRISGMLSWLFRRPIAWGSPATAAAADAEARDDEDAGRAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA