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The map label for this gene is btuB [H]

Identifier: 220918705

GI number: 220918705

Start: 4032337

End: 4034331

Strand: Direct

Name: btuB [H]

Synonym: A2cp1_3613

Alternate gene names: 220918705

Gene position: 4032337-4034331 (Clockwise)

Preceding gene: 220918704

Following gene: 220918706

Centisome position: 80.18

GC content: 78.95

Gene sequence:

>1995_bases
TTGGACTCGAGGCCTCCCCTCGGGCGGCGCGCACGCGCGCTCGCCCTCGTCTTCTCCGCCGTCGCCGGCGTCGCCTCGGG
CGCCGCTGCGCGCGCCGACGATCCGCCCACCAGCGCCGACGCCGGCGCGCCGGATCCCGGCGCCGCGCCGGTGGAGCTCG
ACGAGCTCGTCATCCGCGTCCCCGGGGCCGAGGCGACCCGCGATCCCACCGGCGCGGTCACCGTGGTCGAGGCCGGCCGC
TACGCGGGCGAGGCCAAGGCGGTCGCCGAGCTGGTCGCCACCGCCCCCGGCGTGGCGGTCAGCGCGTACGGCGGCCTCGG
CCAGCTCTCCACCGTGTCCATCCGCGGCTCGAGCGCGAGCGGCGTGCTCGTGCTGCTCGACGGGCTGCCGCTCAACACCG
CGTTCGGCGGCGGCGTGGATCTGTCGACGCTTCCGCGCCAGTGGATCGAGCGCATCGAGGTGCTGCGCGGCGCCGAGGGC
GCGCACTACGGCGCGGGCGCGCTGGGCGGGGTGGTGAACGTGGTCACGCGCGGCGGCGAGCCGGGGGGCTGGTCGGCCGA
GGCGAGCGGGGGATCCTTCGGCACCGCCGCGGCGTCGGCCGAGGCGGCGGCGGGCGGGGAGCGCTGGACGCTGTTCGGCG
CGGCGGGGGCGGAGCGGAGCGACGGGGACTTCACCTACCGGAGGCGCCCTCAGAGCTCGCTGCCCGGCAGCCCGTACCAG
ACGTTCACGCGCGAGAACAACGGCAGCGCCCGCGCCGGCGGGATCGCGAAGCTCACCGCGCGGCTGGGCGAGCGCCGCCT
GGACGCGCTGCTGCAGCTCTCCGGCGGCCGCCGCGAGCTGGCCGGATGGCCCGGCACCGACACCCCCGACGACTGGCAGC
GCGACGCCCGCGCGCTCCTGTCGGTCCGGCTCTCCGGCGCCGGCCCCGCGCCCGGACTCACGCTGGGGGCGCGGCTCACC
GGGCGCGCCGACCTGCTCGACACGCGCATCGGCAGCCTGTCCGGCGGCGACCCGGTGCACCAGCGCGGCGGCGCGGCGGG
CACCGAGGCCGAGGCGGTGCTCCTCCACCCGCACGGCGCGCTCCGGGCCGCGCTGGCCGCGGAGGGCGAGGCGCTCCGCG
CCGACGGGCTGGGCGACACGCGGACCCGCGCGCAGCTCTCCGCCACGCTCTCCGAGGACGCGCGCCTCGCCGCCGGCCGG
GTGCGCCTCTCGCCGGCGGTCCGCGCCGAGCGGGTGGGCCCGTTCGACGGCGTCTCCGCGAAGCTGGGCGGGAGCGTTCG
GGTGGTCGGCCCGCTCTCGGCGCGCGCCAGCGCCGGCCGGACCTTCCGCGCCCCCGGCTTCGCCGAGCTCTACCTGCAGC
TGGGGGGCGCGCTGCCCAATGCGTCGCTCCGCCCCGAGACCGGCCTCTCCGCCGACGCCGCGCTCGTGGCGGAGGGCGCG
CGCGGCATGGCGAGCGTGGGCGGCCACGCGACGCGCTACGAGGACCTCATCGTCTACCAGGCGAGCGCCCTCGGTCGGAT
GAAGCCCTTCAACACCGGCCGCGCCCAGGTCGCCGGCCTGGAGGTCGAGGCCGCCACCACGCCGGCGCCGCGCCTGCTCG
GCCTGTCGCTCGGCGCCGCGTACACCTACCTGCGCACCGAGACGCTGCGCGGCGAGGCGGCCGAGGTGGGCCAGGACCTG
CCCTTCCGCCCCCGCCACCGGCTGTTCGCGCGCGCCGCGATCGCGCCCGGGCCGGCCGAGGCCCACCTCGAGGCCCACCA
CGTGGGCGCGCAGTGGCGGGACGCGCGCAACGCGGACCGCGTCCCCGAGGCGCTGCTCTGGAACGCGGGCGCCTCGGTGC
GCCTCGCCCGCCGGGCCGGGCTGCGGCTCGGGCTCGAGGCGCGCAACCTGCTCGACGACCGCACGCTGACGGACCCCATC
GGAAACCCGCTTCCCGGGCGCATGGTGATGATCACCCTGCGCGCCGGCTCGACCCCAGGAGACCTCACGCCATGA

Upstream 100 bases:

>100_bases
TGCTGCTCCGCGGCCCCGGAAGCCAGGAGACCTCCCTCCGGGTCCGCCGCGAATGGTTCGCGGCACGTGAAGTCCGCGGG
TCCGAAGGAGACCCGGCGAC

Downstream 100 bases:

>100_bases
TCCGGATCCCCCTCCCCGCCCTCCGGGCGGCCCTCGCCGCGGCGGCGCTCGCGCTCGCGGCCTGCAGCAGCGACCCCGAG
ATCGTCTGCGCCGCCGGCGA

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MDSRPPLGRRARALALVFSAVAGVASGAAARADDPPTSADAGAPDPGAAPVELDELVIRVPGAEATRDPTGAVTVVEAGR
YAGEAKAVAELVATAPGVAVSAYGGLGQLSTVSIRGSSASGVLVLLDGLPLNTAFGGGVDLSTLPRQWIERIEVLRGAEG
AHYGAGALGGVVNVVTRGGEPGGWSAEASGGSFGTAAASAEAAAGGERWTLFGAAGAERSDGDFTYRRRPQSSLPGSPYQ
TFTRENNGSARAGGIAKLTARLGERRLDALLQLSGGRRELAGWPGTDTPDDWQRDARALLSVRLSGAGPAPGLTLGARLT
GRADLLDTRIGSLSGGDPVHQRGGAAGTEAEAVLLHPHGALRAALAAEGEALRADGLGDTRTRAQLSATLSEDARLAAGR
VRLSPAVRAERVGPFDGVSAKLGGSVRVVGPLSARASAGRTFRAPGFAELYLQLGGALPNASLRPETGLSADAALVAEGA
RGMASVGGHATRYEDLIVYQASALGRMKPFNTGRAQVAGLEVEAATTPAPRLLGLSLGAAYTYLRTETLRGEAAEVGQDL
PFRPRHRLFARAAIAPGPAEAHLEAHHVGAQWRDARNADRVPEALLWNAGASVRLARRAGLRLGLEARNLLDDRTLTDPI
GNPLPGRMVMITLRAGSTPGDLTP

Sequences:

>Translated_664_residues
MDSRPPLGRRARALALVFSAVAGVASGAAARADDPPTSADAGAPDPGAAPVELDELVIRVPGAEATRDPTGAVTVVEAGR
YAGEAKAVAELVATAPGVAVSAYGGLGQLSTVSIRGSSASGVLVLLDGLPLNTAFGGGVDLSTLPRQWIERIEVLRGAEG
AHYGAGALGGVVNVVTRGGEPGGWSAEASGGSFGTAAASAEAAAGGERWTLFGAAGAERSDGDFTYRRRPQSSLPGSPYQ
TFTRENNGSARAGGIAKLTARLGERRLDALLQLSGGRRELAGWPGTDTPDDWQRDARALLSVRLSGAGPAPGLTLGARLT
GRADLLDTRIGSLSGGDPVHQRGGAAGTEAEAVLLHPHGALRAALAAEGEALRADGLGDTRTRAQLSATLSEDARLAAGR
VRLSPAVRAERVGPFDGVSAKLGGSVRVVGPLSARASAGRTFRAPGFAELYLQLGGALPNASLRPETGLSADAALVAEGA
RGMASVGGHATRYEDLIVYQASALGRMKPFNTGRAQVAGLEVEAATTPAPRLLGLSLGAAYTYLRTETLRGEAAEVGQDL
PFRPRHRLFARAAIAPGPAEAHLEAHHVGAQWRDARNADRVPEALLWNAGASVRLARRAGLRLGLEARNLLDDRTLTDPI
GNPLPGRMVMITLRAGSTPGDLTP
>Mature_664_residues
MDSRPPLGRRARALALVFSAVAGVASGAAARADDPPTSADAGAPDPGAAPVELDELVIRVPGAEATRDPTGAVTVVEAGR
YAGEAKAVAELVATAPGVAVSAYGGLGQLSTVSIRGSSASGVLVLLDGLPLNTAFGGGVDLSTLPRQWIERIEVLRGAEG
AHYGAGALGGVVNVVTRGGEPGGWSAEASGGSFGTAAASAEAAAGGERWTLFGAAGAERSDGDFTYRRRPQSSLPGSPYQ
TFTRENNGSARAGGIAKLTARLGERRLDALLQLSGGRRELAGWPGTDTPDDWQRDARALLSVRLSGAGPAPGLTLGARLT
GRADLLDTRIGSLSGGDPVHQRGGAAGTEAEAVLLHPHGALRAALAAEGEALRADGLGDTRTRAQLSATLSEDARLAAGR
VRLSPAVRAERVGPFDGVSAKLGGSVRVVGPLSARASAGRTFRAPGFAELYLQLGGALPNASLRPETGLSADAALVAEGA
RGMASVGGHATRYEDLIVYQASALGRMKPFNTGRAQVAGLEVEAATTPAPRLLGLSLGAAYTYLRTETLRGEAAEVGQDL
PFRPRHRLFARAAIAPGPAEAHLEAHHVGAQWRDARNADRVPEALLWNAGASVRLARRAGLRLGLEARNLLDDRTLTDPI
GNPLPGRMVMITLRAGSTPGDLTP

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=145, Percent_Identity=40, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786798, Length=196, Percent_Identity=31.6326530612245, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67859; Mature: 67859

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSRPPLGRRARALALVFSAVAGVASGAAARADDPPTSADAGAPDPGAAPVELDELVIRV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
PGAEATRDPTGAVTVVEAGRYAGEAKAVAELVATAPGVAVSAYGGLGQLSTVSIRGSSAS
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCC
GVLVLLDGLPLNTAFGGGVDLSTLPRQWIERIEVLRGAEGAHYGAGALGGVVNVVTRGGE
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC
PGGWSAEASGGSFGTAAASAEAAAGGERWTLFGAAGAERSDGDFTYRRRPQSSLPGSPYQ
CCCCCCCCCCCCCCCHHCCCHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHH
TFTRENNGSARAGGIAKLTARLGERRLDALLQLSGGRRELAGWPGTDTPDDWQRDARALL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SVRLSGAGPAPGLTLGARLTGRADLLDTRIGSLSGGDPVHQRGGAAGTEAEAVLLHPHGA
EEEECCCCCCCCCEECCEECCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEECCCHH
LRAALAAEGEALRADGLGDTRTRAQLSATLSEDARLAAGRVRLSPAVRAERVGPFDGVSA
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHCEEEECHHHHHHCCCCCCCCCC
KLGGSVRVVGPLSARASAGRTFRAPGFAELYLQLGGALPNASLRPETGLSADAALVAEGA
CCCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCC
RGMASVGGHATRYEDLIVYQASALGRMKPFNTGRAQVAGLEVEAATTPAPRLLGLSLGAA
CCHHHCCCCCHHHHEEEEEEHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHH
YTYLRTETLRGEAAEVGQDLPFRPRHRLFARAAIAPGPAEAHLEAHHVGAQWRDARNADR
HHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHCCCCHHH
VPEALLWNAGASVRLARRAGLRLGLEARNLLDDRTLTDPIGNPLPGRMVMITLRAGSTPG
CCHHHHCCCCCCEEEHHHCCCEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCC
DLTP
CCCC
>Mature Secondary Structure
MDSRPPLGRRARALALVFSAVAGVASGAAARADDPPTSADAGAPDPGAAPVELDELVIRV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
PGAEATRDPTGAVTVVEAGRYAGEAKAVAELVATAPGVAVSAYGGLGQLSTVSIRGSSAS
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCC
GVLVLLDGLPLNTAFGGGVDLSTLPRQWIERIEVLRGAEGAHYGAGALGGVVNVVTRGGE
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC
PGGWSAEASGGSFGTAAASAEAAAGGERWTLFGAAGAERSDGDFTYRRRPQSSLPGSPYQ
CCCCCCCCCCCCCCCHHCCCHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHH
TFTRENNGSARAGGIAKLTARLGERRLDALLQLSGGRRELAGWPGTDTPDDWQRDARALL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SVRLSGAGPAPGLTLGARLTGRADLLDTRIGSLSGGDPVHQRGGAAGTEAEAVLLHPHGA
EEEECCCCCCCCCEECCEECCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEECCCHH
LRAALAAEGEALRADGLGDTRTRAQLSATLSEDARLAAGRVRLSPAVRAERVGPFDGVSA
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHCEEEECHHHHHHCCCCCCCCCC
KLGGSVRVVGPLSARASAGRTFRAPGFAELYLQLGGALPNASLRPETGLSADAALVAEGA
CCCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCC
RGMASVGGHATRYEDLIVYQASALGRMKPFNTGRAQVAGLEVEAATTPAPRLLGLSLGAA
CCHHHCCCCCHHHHEEEEEEHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHH
YTYLRTETLRGEAAEVGQDLPFRPRHRLFARAAIAPGPAEAHLEAHHVGAQWRDARNADR
HHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHCCCCHHH
VPEALLWNAGASVRLARRAGLRLGLEARNLLDDRTLTDPIGNPLPGRMVMITLRAGSTPG
CCHHHHCCCCCCEEEHHHCCCEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCC
DLTP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA