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The map label for this gene is pilT [H]

Identifier: 220917998

GI number: 220917998

Start: 3208077

End: 3209189

Strand: Direct

Name: pilT [H]

Synonym: A2cp1_2899

Alternate gene names: 220917998

Gene position: 3208077-3209189 (Clockwise)

Preceding gene: 220917997

Following gene: 220917999

Centisome position: 63.79

GC content: 70.26

Gene sequence:

>1113_bases
ATGGAAGGACAGGGTCCGCTCAGCGAGGAGCTGTTCCACTCGTTCCTGCAGCTCGCCGTGAAGCGCCAGGCGAGCGACGT
GCACTTCGAGGTGGGCTACCCCCCCACCTACCGGGTGTTCGGGGAGCTGCTGGCCGCGAAGTACCCGCCGCTGACGCACG
CGGACACGGAGGCGATCGCGAACTTCGTCCTCCAGGCCCCGGGTTCGGGGTTCACGCCGCTCGACTTCCGCGAGGTGGAC
CGCAGCTACTCGCTGCCCGGGGTGTCGCGCTTCCGCGCGTCCATCTTCAAGCAGCGCGGGAGCTGGGGCGCGGTGATGCG
CACCATCCCGTTCCAGATCCCGGACTTCGACACGCTGAACCTGCCGCCCGTCATCCGCACCATCGCCGAGGCGCGCCGCG
GGCTCATCTGCGTGACCGGCGCCACGGGCAACGGCAAGTCCACCACCATCGCCAGCATCATCAACACGATCATCCAGCAG
GAGCGGCTGCACGTCGTCACGGTCGAGGATCCGATCGAGTTCATCTTCGCCGGCGGCAAGGGCCTCGTGATCCAGCGCGA
GGTCGGATCGGACACGGCCAGCTACAGCGACGCGCTGCGCGCCGCGCTCCGGCAGGACCCCGACATCATCATGGTGGGCG
AGCTGCGCGACCGCGAGGCCGCCGACATCTGCCTGAAGGCGGCGGAGACCGGCCACCTCGTCATCACCTCGCTGCACACG
CCCGACGTGCCCCGCGCGGTCGGGCGCATCGTGGGCCTGTTCCCGGCCGACGAGCAGGACAGCGTCCGGGCGCGGCTCGC
CGACAACCTGCAGGCGGTCATCGCGCTCCGGCTGCTCATGCGCGCCGACGCCACCGGGCTCATCCCCGGGGTCGAGTCGC
TGCTCGCCACCACCAGCGTGCGCGAGCTGATCCGCGACGGCTCCAAGGTCAACGAGCTGCGCACGTACATGGACACCGCC
GGCGCCGACCTGGGCATGCACAGCTTCGATCAGTACCTGTACCGGCTGCACGAGGCGAAGCGCATCGGGCTCGACACCGC
GCTCGCGAACGCGACGCACCGGGCCGACCTGGAGCGGCGGATCATGATGGAGACCGGAGGCCGCCCCGCGTGA

Upstream 100 bases:

>100_bases
AGGAAGGGCCGCGTCGACGGCACCGTCCGGTGAGGAAGCGGGGGCGCGGCCCGCGACCTTGCTGGCCGCGCCCTCCGGTT
GACGCTAGACTCACCGTCTT

Downstream 100 bases:

>100_bases
TCGCCGCCGGCGCCCCAGCGGCGTCGCGCGTTCTGGTGGTGGGCTCGGACGCCGACGTCTCCATCGCGCTGCACCTGCAC
CTCGAGCGTGCGGGGCACGC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MEGQGPLSEELFHSFLQLAVKRQASDVHFEVGYPPTYRVFGELLAAKYPPLTHADTEAIANFVLQAPGSGFTPLDFREVD
RSYSLPGVSRFRASIFKQRGSWGAVMRTIPFQIPDFDTLNLPPVIRTIAEARRGLICVTGATGNGKSTTIASIINTIIQQ
ERLHVVTVEDPIEFIFAGGKGLVIQREVGSDTASYSDALRAALRQDPDIIMVGELRDREAADICLKAAETGHLVITSLHT
PDVPRAVGRIVGLFPADEQDSVRARLADNLQAVIALRLLMRADATGLIPGVESLLATTSVRELIRDGSKVNELRTYMDTA
GADLGMHSFDQYLYRLHEAKRIGLDTALANATHRADLERRIMMETGGRPA

Sequences:

>Translated_370_residues
MEGQGPLSEELFHSFLQLAVKRQASDVHFEVGYPPTYRVFGELLAAKYPPLTHADTEAIANFVLQAPGSGFTPLDFREVD
RSYSLPGVSRFRASIFKQRGSWGAVMRTIPFQIPDFDTLNLPPVIRTIAEARRGLICVTGATGNGKSTTIASIINTIIQQ
ERLHVVTVEDPIEFIFAGGKGLVIQREVGSDTASYSDALRAALRQDPDIIMVGELRDREAADICLKAAETGHLVITSLHT
PDVPRAVGRIVGLFPADEQDSVRARLADNLQAVIALRLLMRADATGLIPGVESLLATTSVRELIRDGSKVNELRTYMDTA
GADLGMHSFDQYLYRLHEAKRIGLDTALANATHRADLERRIMMETGGRPA
>Mature_370_residues
MEGQGPLSEELFHSFLQLAVKRQASDVHFEVGYPPTYRVFGELLAAKYPPLTHADTEAIANFVLQAPGSGFTPLDFREVD
RSYSLPGVSRFRASIFKQRGSWGAVMRTIPFQIPDFDTLNLPPVIRTIAEARRGLICVTGATGNGKSTTIASIINTIIQQ
ERLHVVTVEDPIEFIFAGGKGLVIQREVGSDTASYSDALRAALRQDPDIIMVGELRDREAADICLKAAETGHLVITSLHT
PDVPRAVGRIVGLFPADEQDSVRARLADNLQAVIALRLLMRADATGLIPGVESLLATTSVRELIRDGSKVNELRTYMDTA
GADLGMHSFDQYLYRLHEAKRIGLDTALANATHRADLERRIMMETGGRPA

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=324, Percent_Identity=38.2716049382716, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786296, Length=285, Percent_Identity=32.280701754386, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789723, Length=257, Percent_Identity=32.6848249027237, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40518; Mature: 40518

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQGPLSEELFHSFLQLAVKRQASDVHFEVGYPPTYRVFGELLAAKYPPLTHADTEAIA
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
NFVLQAPGSGFTPLDFREVDRSYSLPGVSRFRASIFKQRGSWGAVMRTIPFQIPDFDTLN
HHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCC
LPPVIRTIAEARRGLICVTGATGNGKSTTIASIINTIIQQERLHVVTVEDPIEFIFAGGK
CCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCC
GLVIQREVGSDTASYSDALRAALRQDPDIIMVGELRDREAADICLKAAETGHLVITSLHT
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCC
PDVPRAVGRIVGLFPADEQDSVRARLADNLQAVIALRLLMRADATGLIPGVESLLATTSV
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
RELIRDGSKVNELRTYMDTAGADLGMHSFDQYLYRLHEAKRIGLDTALANATHRADLERR
HHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IMMETGGRPA
HHHHCCCCCC
>Mature Secondary Structure
MEGQGPLSEELFHSFLQLAVKRQASDVHFEVGYPPTYRVFGELLAAKYPPLTHADTEAIA
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
NFVLQAPGSGFTPLDFREVDRSYSLPGVSRFRASIFKQRGSWGAVMRTIPFQIPDFDTLN
HHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCC
LPPVIRTIAEARRGLICVTGATGNGKSTTIASIINTIIQQERLHVVTVEDPIEFIFAGGK
CCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCC
GLVIQREVGSDTASYSDALRAALRQDPDIIMVGELRDREAADICLKAAETGHLVITSLHT
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCC
PDVPRAVGRIVGLFPADEQDSVRARLADNLQAVIALRLLMRADATGLIPGVESLLATTSV
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
RELIRDGSKVNELRTYMDTAGADLGMHSFDQYLYRLHEAKRIGLDTALANATHRADLERR
HHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IMMETGGRPA
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]