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The map label for this gene is pncA [H]

Identifier: 220917737

GI number: 220917737

Start: 2910668

End: 2911309

Strand: Reverse

Name: pncA [H]

Synonym: A2cp1_2638

Alternate gene names: 220917737

Gene position: 2911309-2910668 (Counterclockwise)

Preceding gene: 220917740

Following gene: 220917735

Centisome position: 57.89

GC content: 76.48

Gene sequence:

>642_bases
ATGCGGATCGATCCCGGGTCGGATGCGCTCCTGGTGGTGGACCTCCAGCACGACTTCCTGCCGGGCGGCGCGCTCGGCGT
CGCCGAGGGCGACCGGATCGTGGAGCCGCTGGCGCGCCTGGCGCCCGCGTTCTCGACCGTGGTGGCGACGCAGGACTGGC
ATCCGCCCGGCCACGTGTCCTTCGCCTCCACGCACCCGGGGCGCGAGCCCTACGCGTCCATCGCGCTCTCGCAGGGGCCG
CAGGAGCTCTGGCCGGACCACTGCGTCCGCGGCACGCGCGGCGCCGCCCTGCACCCGGCGCTCCCCGACGCGGCGGTCAC
GCTGGTGCTGCGCAAGGGCACGCGGCGCGAGGTGGACTCCTACAGCGCCTTCCGCGAGAACGTCGGCCCGGACGGCCGGC
GCCCGTCCACCGGGCTGGGCGCCTGGCTCTCCGCCCGCGGCGTGCGCCGCCTGTTCCTCGGCGGGCTGGCGCGCGACTTC
TGCGTGCGGGTGTCCGCCGTCGACGCGGCTGTAGAGGGCTTCGAGGTGGTGGTGCTCGACGACCTGACGCGCGCGGTGTT
CCCCGAGCGGCGCGACGAGGTGGACCGGGAGCTCGCGGCCGCGGGCGCGCGGCTGGCGGTCAGCCAGGACCTGATCCGCT
GA

Upstream 100 bases:

>100_bases
GCCGCTCCGGCGTGTGCGACGGCGCTCGCGGCGGCCCCGCCGGGGGACGCGGCGCGGCACCTCCGCGGACGATCCGCCAC
GCCGGTGTTATGGGGTGCCC

Downstream 100 bases:

>100_bases
GGCGGTGCTCAGAACAGCGCGCGCTGGAGCGGCTCGGGCGGCGGCGCGAGGCAGGCGGCGTCGTCGTTCGCGGGCGAGTT
GACGCGCGGTGACACGGGGC

Product: Nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRIDPGSDALLVVDLQHDFLPGGALGVAEGDRIVEPLARLAPAFSTVVATQDWHPPGHVSFASTHPGREPYASIALSQGP
QELWPDHCVRGTRGAALHPALPDAAVTLVLRKGTRREVDSYSAFRENVGPDGRRPSTGLGAWLSARGVRRLFLGGLARDF
CVRVSAVDAAVEGFEVVVLDDLTRAVFPERRDEVDRELAAAGARLAVSQDLIR

Sequences:

>Translated_213_residues
MRIDPGSDALLVVDLQHDFLPGGALGVAEGDRIVEPLARLAPAFSTVVATQDWHPPGHVSFASTHPGREPYASIALSQGP
QELWPDHCVRGTRGAALHPALPDAAVTLVLRKGTRREVDSYSAFRENVGPDGRRPSTGLGAWLSARGVRRLFLGGLARDF
CVRVSAVDAAVEGFEVVVLDDLTRAVFPERRDEVDRELAAAGARLAVSQDLIR
>Mature_213_residues
MRIDPGSDALLVVDLQHDFLPGGALGVAEGDRIVEPLARLAPAFSTVVATQDWHPPGHVSFASTHPGREPYASIALSQGP
QELWPDHCVRGTRGAALHPALPDAAVTLVLRKGTRREVDSYSAFRENVGPDGRRPSTGLGAWLSARGVRRLFLGGLARDF
CVRVSAVDAAVEGFEVVVLDDLTRAVFPERRDEVDRELAAAGARLAVSQDLIR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=208, Percent_Identity=45.1923076923077, Blast_Score=160, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193206735, Length=229, Percent_Identity=29.6943231441048, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17543130, Length=229, Percent_Identity=29.6943231441048, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71999339, Length=219, Percent_Identity=29.6803652968037, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=216, Percent_Identity=31.9444444444444, Blast_Score=117, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648247, Length=201, Percent_Identity=35.8208955223881, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22870; Mature: 22870

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDPGSDALLVVDLQHDFLPGGALGVAEGDRIVEPLARLAPAFSTVVATQDWHPPGHVS
CCCCCCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
FASTHPGREPYASIALSQGPQELWPDHCVRGTRGAALHPALPDAAVTLVLRKGTRREVDS
ECCCCCCCCHHHHHHHCCCCHHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHH
YSAFRENVGPDGRRPSTGLGAWLSARGVRRLFLGGLARDFCVRVSAVDAAVEGFEVVVLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
DLTRAVFPERRDEVDRELAAAGARLAVSQDLIR
HHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCC
>Mature Secondary Structure
MRIDPGSDALLVVDLQHDFLPGGALGVAEGDRIVEPLARLAPAFSTVVATQDWHPPGHVS
CCCCCCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
FASTHPGREPYASIALSQGPQELWPDHCVRGTRGAALHPALPDAAVTLVLRKGTRREVDS
ECCCCCCCCHHHHHHHCCCCHHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHH
YSAFRENVGPDGRRPSTGLGAWLSARGVRRLFLGGLARDFCVRVSAVDAAVEGFEVVVLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
DLTRAVFPERRDEVDRELAAAGARLAVSQDLIR
HHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]