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The map label for this gene is cca [H]

Identifier: 220917575

GI number: 220917575

Start: 2732752

End: 2734062

Strand: Reverse

Name: cca [H]

Synonym: A2cp1_2475

Alternate gene names: 220917575

Gene position: 2734062-2732752 (Counterclockwise)

Preceding gene: 220917576

Following gene: 220917574

Centisome position: 54.36

GC content: 77.42

Gene sequence:

>1311_bases
ATGCGGCCGCCCGCCGCCCTCGAGCAGGCGCGCTTCCCCGAGGCGATCCTGGACGTGCTCCGCCGCCTCTCGGCCGCGGG
GCACCGCTCCTGGCTGGTCGGCGGGGCGGTCCGCGACCTGCTCCTGCACCGCCCCCGCGCCGCCACCGACTTCGACGTCG
CCACGCCGGCCACGCCGCGGCAGGTCATGGCGCTGTTCCGGCGCGTCATCCCGACGGGCATCGACCACGGCACCGTCACC
GTGCTGGTGGGCGACGAGAAGGTCGAGGTCACCACGTTCCGCGGGGAGGGCGACTACGTGGACGGCCGCCGTCCGGCCTC
GGTGACGTTCCTGGCCGAGCTCGAGGCGGACCTGGCCCGGCGCGACTTCACCATGAACGCGATGGCGTACGATCCGCTCC
AGGCGGACTTCCGCGATCCGTTCGACGGCCGCGTGGACATGAGCCGCCGGCGCATCCGCGCGGTCGGCGAGCCGGCCGCG
CGCTTCGGCGAGGACGGCCTCCGCGCCATGCGCGCCGTCCGCTTCGCGGCGCAGCTCGGCTACCGCCTCGACCCTGCCAC
CCGCGCCGCCATCCCCGGCGCGCTCGAGGTGGTCCGCAAGGTGAGCGTGGAGCGCGTCGCCGAGGAGCTGTCGCGGCTCG
TGCTCGCCGCGCACGCCGGGCCCGCGCTGCTGCTGCTCCGCCGCACCGGGCTCCTCGGGGTGGTGCTGCCCGCCATCGCC
GCGCTCCCGCTCTCCGCCTACCGGCACGCGGCCTCGGTGGTCCGTCACGCCCCCGCCGAGCCGGCGCTGCGGCTCGCGGC
GCTGCTGCACGGGGTTGGCCCCGAGGAGGCCTCGAAGATCGTGTTCGGCCTGCGGCTCCCCCGCCGCCTGTCCGACGAGG
TCGGGACGCTCCTCCGGGCGTACGCCTGCCCGGCGGCGCGCCACCCGGCCGCGCTGCCGGACGCGCCGGCGGACATCCGG
CGGTGGCTCGCGCGCACGGGCCCGCAGCGCGTGCCGGCGGTGTTCGAGCTGGTGTCGGCGGAGCTCCGCACGCTCTCGCC
CGCGCACCGCGCCGCGGTCGCGCCGGAGGTCTCGCGGGCGCGCGCGGCCATCGACGCCGAGCTCGCGGCGGCGCCACCGC
TCGCCACCGGCGACCTCGCCCTGGATGGGCGTGCGGTGATGTCGCTGCTGGGTGAGGGGCCCGGTCCCCACGTCGGCGAG
GCGCTCCACCACCTGCTCGACCGGGTGCTGGAGGATCCTGCGCAGAACACGCGCGAGGCGCTCGCGGCCGAGCTGCGCCG
ATGGTGGGCCGGTCGGACCGGGGCCTTATAA

Upstream 100 bases:

>100_bases
GCGCGGTGGGAACGCTCGACGAGGGCACGCGCCTCGCGCTCATCCGCCTCTACTTCCGCCTCCTCGATCGCGCCGGCGAG
GAGCTGCGCAGGATGCACTG

Downstream 100 bases:

>100_bases
TTCAGGCGGGGTTTCGCGATGCCGCGGGTGCTGCTCATCGAGGAGGACGGGCCGCGCCGCGACCGGGCGCGAGCCCTGCT
CGAGGCCGAGGGGTTCGCGG

Product: tRNA cytidylyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRPPAALEQARFPEAILDVLRRLSAAGHRSWLVGGAVRDLLLHRPRAATDFDVATPATPRQVMALFRRVIPTGIDHGTVT
VLVGDEKVEVTTFRGEGDYVDGRRPASVTFLAELEADLARRDFTMNAMAYDPLQADFRDPFDGRVDMSRRRIRAVGEPAA
RFGEDGLRAMRAVRFAAQLGYRLDPATRAAIPGALEVVRKVSVERVAEELSRLVLAAHAGPALLLLRRTGLLGVVLPAIA
ALPLSAYRHAASVVRHAPAEPALRLAALLHGVGPEEASKIVFGLRLPRRLSDEVGTLLRAYACPAARHPAALPDAPADIR
RWLARTGPQRVPAVFELVSAELRTLSPAHRAAVAPEVSRARAAIDAELAAAPPLATGDLALDGRAVMSLLGEGPGPHVGE
ALHHLLDRVLEDPAQNTREALAAELRRWWAGRTGAL

Sequences:

>Translated_436_residues
MRPPAALEQARFPEAILDVLRRLSAAGHRSWLVGGAVRDLLLHRPRAATDFDVATPATPRQVMALFRRVIPTGIDHGTVT
VLVGDEKVEVTTFRGEGDYVDGRRPASVTFLAELEADLARRDFTMNAMAYDPLQADFRDPFDGRVDMSRRRIRAVGEPAA
RFGEDGLRAMRAVRFAAQLGYRLDPATRAAIPGALEVVRKVSVERVAEELSRLVLAAHAGPALLLLRRTGLLGVVLPAIA
ALPLSAYRHAASVVRHAPAEPALRLAALLHGVGPEEASKIVFGLRLPRRLSDEVGTLLRAYACPAARHPAALPDAPADIR
RWLARTGPQRVPAVFELVSAELRTLSPAHRAAVAPEVSRARAAIDAELAAAPPLATGDLALDGRAVMSLLGEGPGPHVGE
ALHHLLDRVLEDPAQNTREALAAELRRWWAGRTGAL
>Mature_436_residues
MRPPAALEQARFPEAILDVLRRLSAAGHRSWLVGGAVRDLLLHRPRAATDFDVATPATPRQVMALFRRVIPTGIDHGTVT
VLVGDEKVEVTTFRGEGDYVDGRRPASVTFLAELEADLARRDFTMNAMAYDPLQADFRDPFDGRVDMSRRRIRAVGEPAA
RFGEDGLRAMRAVRFAAQLGYRLDPATRAAIPGALEVVRKVSVERVAEELSRLVLAAHAGPALLLLRRTGLLGVVLPAIA
ALPLSAYRHAASVVRHAPAEPALRLAALLHGVGPEEASKIVFGLRLPRRLSDEVGTLLRAYACPAARHPAALPDAPADIR
RWLARTGPQRVPAVFELVSAELRTLSPAHRAAVAPEVSRARAAIDAELAAAPPLATGDLALDGRAVMSLLGEGPGPHVGE
ALHHLLDRVLEDPAQNTREALAAELRRWWAGRTGAL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI87081691, Length=250, Percent_Identity=37.6, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789436, Length=226, Percent_Identity=34.070796460177, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=201, Percent_Identity=35.8208955223881, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281360112, Length=251, Percent_Identity=34.2629482071713, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357337, Length=251, Percent_Identity=34.2629482071713, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46983; Mature: 46983

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPAALEQARFPEAILDVLRRLSAAGHRSWLVGGAVRDLLLHRPRAATDFDVATPATPR
CCCCHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
QVMALFRRVIPTGIDHGTVTVLVGDEKVEVTTFRGEGDYVDGRRPASVTFLAELEADLAR
HHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHH
RDFTMNAMAYDPLQADFRDPFDGRVDMSRRRIRAVGEPAARFGEDGLRAMRAVRFAAQLG
HCCCCCHHCCCCCHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHC
YRLDPATRAAIPGALEVVRKVSVERVAEELSRLVLAAHAGPALLLLRRTGLLGVVLPAIA
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
ALPLSAYRHAASVVRHAPAEPALRLAALLHGVGPEEASKIVFGLRLPRRLSDEVGTLLRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
YACPAARHPAALPDAPADIRRWLARTGPQRVPAVFELVSAELRTLSPAHRAAVAPEVSRA
HHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHH
RAAIDAELAAAPPLATGDLALDGRAVMSLLGEGPGPHVGEALHHLLDRVLEDPAQNTREA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
LAAELRRWWAGRTGAL
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRPPAALEQARFPEAILDVLRRLSAAGHRSWLVGGAVRDLLLHRPRAATDFDVATPATPR
CCCCHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
QVMALFRRVIPTGIDHGTVTVLVGDEKVEVTTFRGEGDYVDGRRPASVTFLAELEADLAR
HHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHH
RDFTMNAMAYDPLQADFRDPFDGRVDMSRRRIRAVGEPAARFGEDGLRAMRAVRFAAQLG
HCCCCCHHCCCCCHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHC
YRLDPATRAAIPGALEVVRKVSVERVAEELSRLVLAAHAGPALLLLRRTGLLGVVLPAIA
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
ALPLSAYRHAASVVRHAPAEPALRLAALLHGVGPEEASKIVFGLRLPRRLSDEVGTLLRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
YACPAARHPAALPDAPADIRRWLARTGPQRVPAVFELVSAELRTLSPAHRAAVAPEVSRA
HHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHH
RAAIDAELAAAPPLATGDLALDGRAVMSLLGEGPGPHVGEALHHLLDRVLEDPAQNTREA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
LAAELRRWWAGRTGAL
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA