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The map label for this gene is emrB [H]

Identifier: 220917478

GI number: 220917478

Start: 2637243

End: 2638832

Strand: Reverse

Name: emrB [H]

Synonym: A2cp1_2378

Alternate gene names: 220917478

Gene position: 2638832-2637243 (Counterclockwise)

Preceding gene: 220917479

Following gene: 220917477

Centisome position: 52.47

GC content: 70.75

Gene sequence:

>1590_bases
GTGAGCGCCGCGCGCGCGCCCACCAACAAGTGGCTGGTGACCGTGTCGGTCACCTTCGGCACGCTCATGGGCGCAATCGA
CGCGTCCATCGTGAACGTGGCGCTCCCGCAGATCCGCGGCGCGGTCGGCGCGACCGTGCAGGAGATCACCTGGATCACCA
CCGGCTTCGCCATCGCCACGGTGATCGTGATGCCGCTGACCGCGTTCCTGGGCCGCCTGTTCGGGCAGAAGCGCGTCTAC
ATGGCGAGCCTGCTCGTGTTCGTGGTCGGCTCGGCGCTCTGCGGCCTGGCCTCCAGCCTCGGCCAGGTGGTGGCCTTCCG
CGCCATCCAGGGGCTCGGCGCGGGCGCGCTGCAGCCCACCGAGCAGGCCATCCTGCGCCAGACCTTCCCGCCCAAGGAGC
AGGGCATGGCCATGGCGCTGTTCGCGATGGCGGTGATGGTGGGCCCCGCCGTCGGCCCGACCCTGGGCGGCTACATCGTG
GACAACTTCCACTGGTCCTGGATCTTCTTCATCAACCTGCCGGTGGGCGCGCTGGGCCTGTTCATGGTGTGGCGCTTCGT
GCACGAGCCGGAGGACATCCGCGCCGCGAACGCGGCCGCGGCGGCCGAGCAGCGGCGGAACATGGACTGGCTCGGCATCG
CGCTCCTCGCGGTCGGGCTCGCGACGCTCCAGTACTTCCTCGAGGAGGGGCAGCGGGACGACTGGTTCGAGTCGCGCACC
ATCACCGCGGTGGCGGCGGTGGCCGCCGTCGCGCTGGCGCTGTTCGTCTGGCGCGAGCTGGTGGCGAAGGTGCCCGCGGT
GAACCTGCGGCTGTTCGGCGACGCGGTGTTCACCTCCGGCACGGTCATCGGCGCCGTCATGTTCGCGATCCTGATGGCGA
GCATGTTCCTGCTGCCGCTGTTCATGCAGGAGCTGCTCGGCTTCACCGCCATGCAGTCCGGCCTCGCGCTCATGCCGCGC
GTGCTGGTGATGATGCTGGTCACGCCCATCGTCGGCCGGCTCTACTCGCGCGTGTCGCCGCGGCTGCTGGTCGCCATCGG
CGTGGTGCTGGTGTCGCTCGGCTCCTTCGACGTCTCGCGCATCACGCTGGAGAGCACCTCCTCCGGCATCGTCCACGCGA
TCCTGATCCAGGGGGTGGGCTTCAGCTTCCTGTTCGTGCCGCTCACCACCGCCGCGCTCTCCAACGTGCCGCGCGCCCGG
CTGGCGGACGCGACCGGGCTGAACTCGCTGCTGCGCCAGGTGGGCGCGTCGATGGGCCTCGCCATCTTCGCGACGCTGCT
CGACCGCGCCGGCGTCCACGCCCGGGCCGCGGTCGCGAGCCACCTCGGGCCCGAGCGGCCGGAGGTGCAGGACCGGCTCG
GGCAGCTGGCGGCGTTCTTCGCGCAGCGGGGCTTCGACCCGGTCTCCGCCCGCGAGGCCGCGCTCCGGGCGCTGGACGGC
ATCGTGCGCAAGCAGTCGATGGTGATCGCCTTCGAGCACGTGCTGTTCGTCACCGGCGTCCTGTTCCTGTGCGTGCTCCC
GCTCCTCTACTTCCTCAAGGTGAACCGGCACGGACCGCGCGAGAAGGTCCACGTCGAGATGGAGATGTGA

Upstream 100 bases:

>100_bases
GGATCACCCGGGCGCTGGTGGTGCGCACGCTGGAACGGCAGGGCAGCTGGGACGGCTTCGTGGACGCGGCGCTCGACGCG
TTCCTGCGCGGGGCGGGGCG

Downstream 100 bases:

>100_bases
CCATGACCCCTCCCGCCGATCCCAGGCTCCACGAGGTCCCGAGCTCCCCCGCGCCGGCGGCCGCCCCGGCGCGTCCGGGC
GGCCGGCGCCGCGTGCTCGT

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSAARAPTNKWLVTVSVTFGTLMGAIDASIVNVALPQIRGAVGATVQEITWITTGFAIATVIVMPLTAFLGRLFGQKRVY
MASLLVFVVGSALCGLASSLGQVVAFRAIQGLGAGALQPTEQAILRQTFPPKEQGMAMALFAMAVMVGPAVGPTLGGYIV
DNFHWSWIFFINLPVGALGLFMVWRFVHEPEDIRAANAAAAAEQRRNMDWLGIALLAVGLATLQYFLEEGQRDDWFESRT
ITAVAAVAAVALALFVWRELVAKVPAVNLRLFGDAVFTSGTVIGAVMFAILMASMFLLPLFMQELLGFTAMQSGLALMPR
VLVMMLVTPIVGRLYSRVSPRLLVAIGVVLVSLGSFDVSRITLESTSSGIVHAILIQGVGFSFLFVPLTTAALSNVPRAR
LADATGLNSLLRQVGASMGLAIFATLLDRAGVHARAAVASHLGPERPEVQDRLGQLAAFFAQRGFDPVSAREAALRALDG
IVRKQSMVIAFEHVLFVTGVLFLCVLPLLYFLKVNRHGPREKVHVEMEM

Sequences:

>Translated_529_residues
MSAARAPTNKWLVTVSVTFGTLMGAIDASIVNVALPQIRGAVGATVQEITWITTGFAIATVIVMPLTAFLGRLFGQKRVY
MASLLVFVVGSALCGLASSLGQVVAFRAIQGLGAGALQPTEQAILRQTFPPKEQGMAMALFAMAVMVGPAVGPTLGGYIV
DNFHWSWIFFINLPVGALGLFMVWRFVHEPEDIRAANAAAAAEQRRNMDWLGIALLAVGLATLQYFLEEGQRDDWFESRT
ITAVAAVAAVALALFVWRELVAKVPAVNLRLFGDAVFTSGTVIGAVMFAILMASMFLLPLFMQELLGFTAMQSGLALMPR
VLVMMLVTPIVGRLYSRVSPRLLVAIGVVLVSLGSFDVSRITLESTSSGIVHAILIQGVGFSFLFVPLTTAALSNVPRAR
LADATGLNSLLRQVGASMGLAIFATLLDRAGVHARAAVASHLGPERPEVQDRLGQLAAFFAQRGFDPVSAREAALRALDG
IVRKQSMVIAFEHVLFVTGVLFLCVLPLLYFLKVNRHGPREKVHVEMEM
>Mature_528_residues
SAARAPTNKWLVTVSVTFGTLMGAIDASIVNVALPQIRGAVGATVQEITWITTGFAIATVIVMPLTAFLGRLFGQKRVYM
ASLLVFVVGSALCGLASSLGQVVAFRAIQGLGAGALQPTEQAILRQTFPPKEQGMAMALFAMAVMVGPAVGPTLGGYIVD
NFHWSWIFFINLPVGALGLFMVWRFVHEPEDIRAANAAAAAEQRRNMDWLGIALLAVGLATLQYFLEEGQRDDWFESRTI
TAVAAVAAVALALFVWRELVAKVPAVNLRLFGDAVFTSGTVIGAVMFAILMASMFLLPLFMQELLGFTAMQSGLALMPRV
LVMMLVTPIVGRLYSRVSPRLLVAIGVVLVSLGSFDVSRITLESTSSGIVHAILIQGVGFSFLFVPLTTAALSNVPRARL
ADATGLNSLLRQVGASMGLAIFATLLDRAGVHARAAVASHLGPERPEVQDRLGQLAAFFAQRGFDPVSAREAALRALDGI
VRKQSMVIAFEHVLFVTGVLFLCVLPLLYFLKVNRHGPREKVHVEMEM

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=32.4752475247525, Blast_Score=281, Evalue=1e-76,
Organism=Escherichia coli, GI1788710, Length=504, Percent_Identity=28.968253968254, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87081983, Length=429, Percent_Identity=29.3706293706294, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1790195, Length=413, Percent_Identity=26.8765133171913, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788392, Length=430, Percent_Identity=26.9767441860465, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322958, Length=503, Percent_Identity=23.2604373757455, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=232, Percent_Identity=24.1379310344828, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319770, Length=502, Percent_Identity=23.3067729083665, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=25.6578947368421, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56780; Mature: 56649

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00369 PTS_HPR_HIS ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARAPTNKWLVTVSVTFGTLMGAIDASIVNVALPQIRGAVGATVQEITWITTGFAIAT
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VIVMPLTAFLGRLFGQKRVYMASLLVFVVGSALCGLASSLGQVVAFRAIQGLGAGALQPT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EQAILRQTFPPKEQGMAMALFAMAVMVGPAVGPTLGGYIVDNFHWSWIFFINLPVGALGL
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCHHHHHH
FMVWRFVHEPEDIRAANAAAAAEQRRNMDWLGIALLAVGLATLQYFLEEGQRDDWFESRT
HHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ITAVAAVAAVALALFVWRELVAKVPAVNLRLFGDAVFTSGTVIGAVMFAILMASMFLLPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FMQELLGFTAMQSGLALMPRVLVMMLVTPIVGRLYSRVSPRLLVAIGVVLVSLGSFDVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCE
ITLESTSSGIVHAILIQGVGFSFLFVPLTTAALSNVPRARLADATGLNSLLRQVGASMGL
EEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHH
AIFATLLDRAGVHARAAVASHLGPERPEVQDRLGQLAAFFAQRGFDPVSAREAALRALDG
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IVRKQSMVIAFEHVLFVTGVLFLCVLPLLYFLKVNRHGPREKVHVEMEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure 
SAARAPTNKWLVTVSVTFGTLMGAIDASIVNVALPQIRGAVGATVQEITWITTGFAIAT
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VIVMPLTAFLGRLFGQKRVYMASLLVFVVGSALCGLASSLGQVVAFRAIQGLGAGALQPT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EQAILRQTFPPKEQGMAMALFAMAVMVGPAVGPTLGGYIVDNFHWSWIFFINLPVGALGL
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCHHHHHH
FMVWRFVHEPEDIRAANAAAAAEQRRNMDWLGIALLAVGLATLQYFLEEGQRDDWFESRT
HHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ITAVAAVAAVALALFVWRELVAKVPAVNLRLFGDAVFTSGTVIGAVMFAILMASMFLLPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FMQELLGFTAMQSGLALMPRVLVMMLVTPIVGRLYSRVSPRLLVAIGVVLVSLGSFDVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCE
ITLESTSSGIVHAILIQGVGFSFLFVPLTTAALSNVPRARLADATGLNSLLRQVGASMGL
EEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHH
AIFATLLDRAGVHARAAVASHLGPERPEVQDRLGQLAAFFAQRGFDPVSAREAALRALDG
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IVRKQSMVIAFEHVLFVTGVLFLCVLPLLYFLKVNRHGPREKVHVEMEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]