Click here to switch to the map view.

The map label for this gene is ald [H]

Identifier: 220916951

GI number: 220916951

Start: 2066305

End: 2067417

Strand: Direct

Name: ald [H]

Synonym: A2cp1_1847

Alternate gene names: 220916951

Gene position: 2066305-2067417 (Clockwise)

Preceding gene: 220916948

Following gene: 220916955

Centisome position: 41.09

GC content: 73.14

Gene sequence:

>1113_bases
ATGCGCGTCGGCTGCCCCAAGGAGATCAAGAACAACGAGAACCGCGTCGGCCTGACGCCCGGCGGGGCGCGATCGCTGGT
CGCGGCCGGCCACGAGGTCCTGGTCGAGTCCGGCGCCGGCGAGCGGAGCGGGTTCACCGACGCCGAGTACGAGGCGGTCG
GCGCGCGCATCGCGCCGGTGGCGCGGCAGGTGTTCGAGCAGGCCGAGCTGATCGTGAAGGTGAAGGAGCCGCAGCCGCCC
GAGCTGGCCGCGCTCCGGCCCGGGCAGATCCTGTTCACCTACCTGCACCTCGCGCCGGACCCGCTCCAGACGAAGGCGCT
CCTCGAGCACGAGGTCACCGCCATCGCCTACGAGACCATCCGCGACGCCTCCGGGCGGCTGCCGCTGCTCACGCCCATGT
CCGAGGTGGCCGGCCGCATGGCCGCGCACGTGGGCGCGTTCTACCTGCAGCAGCCGAACGGCGGGCGCGGCATGCTGCTC
GGCGGCGTGCCCGGGGTGCCGCCCGCGCACGTGGTGGTGCTGGGCGGCGGGGTGGTGGGCCTGAACGCCATCAAGGTCGC
GGCCGGCATGGGCGCGCGGGTCACCGTCCTCGACAAGAGCCTGCCCACGCTCCGCTACCTCGACGACATCTTCGGCAACC
GCATCGAGACGCTCTGGTCGAACGAGGCCCACGTGGAGCAGGCGCTGGTCCAGGCCGACCTCGTCATCGGCGCGGTGCTC
ATCCCCGGCGCCGCCGCGCCACGGCTGGTGCAGCGGCGCATGCTGAAGGACATGAAGCCGGGCTGCGTGATCGTGGACGT
GGCGGTGGACCAGGGCGGCTGCGTCGAGACCACCCGGCCGACCACCCACGCCGACCCGGTCTACTTCGTGGACGGCGTGC
TGCACTACGCGGTCGCGAACATGCCCGGCGCGCTGCCGCGGACCTCGACGCTCGCGCTCACCAACGCGACGCTCCCGTAC
GCGATGCAGCTCGCCGGCCGCGGCGTGAAGGACGCGCTCCTCGCGGATCCCGGCTTCCTGGCCGGGCTCAACACGTACCG
GGGCAAGCTCACCTGCGCCCCGGCGGCGGAGAGCCTGGGGATCCCGTACACGGATCCGCGGACGCTGCTGTAG

Upstream 100 bases:

>100_bases
GGGACGCGCCATCGCGAACACCCGGTGCGGGGGTCGACGGCGGCCCTCGTTCGTCTTACTCGGTGGGGGCCGCTCGGCCC
TCACCACCCCGGAGCTTCGC

Downstream 100 bases:

>100_bases
GGGCCCCTCCCGCCGCGGTCCGTCCCTCGACTTCGCGCGGCATTCGCCGCGCTACGCTCGGGATGAGCGGGGATCACTCC
GCTCGCCCCGAGCCCGTCGA

Product: alanine dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MRVGCPKEIKNNENRVGLTPGGARSLVAAGHEVLVESGAGERSGFTDAEYEAVGARIAPVARQVFEQAELIVKVKEPQPP
ELAALRPGQILFTYLHLAPDPLQTKALLEHEVTAIAYETIRDASGRLPLLTPMSEVAGRMAAHVGAFYLQQPNGGRGMLL
GGVPGVPPAHVVVLGGGVVGLNAIKVAAGMGARVTVLDKSLPTLRYLDDIFGNRIETLWSNEAHVEQALVQADLVIGAVL
IPGAAAPRLVQRRMLKDMKPGCVIVDVAVDQGGCVETTRPTTHADPVYFVDGVLHYAVANMPGALPRTSTLALTNATLPY
AMQLAGRGVKDALLADPGFLAGLNTYRGKLTCAPAAESLGIPYTDPRTLL

Sequences:

>Translated_370_residues
MRVGCPKEIKNNENRVGLTPGGARSLVAAGHEVLVESGAGERSGFTDAEYEAVGARIAPVARQVFEQAELIVKVKEPQPP
ELAALRPGQILFTYLHLAPDPLQTKALLEHEVTAIAYETIRDASGRLPLLTPMSEVAGRMAAHVGAFYLQQPNGGRGMLL
GGVPGVPPAHVVVLGGGVVGLNAIKVAAGMGARVTVLDKSLPTLRYLDDIFGNRIETLWSNEAHVEQALVQADLVIGAVL
IPGAAAPRLVQRRMLKDMKPGCVIVDVAVDQGGCVETTRPTTHADPVYFVDGVLHYAVANMPGALPRTSTLALTNATLPY
AMQLAGRGVKDALLADPGFLAGLNTYRGKLTCAPAAESLGIPYTDPRTLL
>Mature_370_residues
MRVGCPKEIKNNENRVGLTPGGARSLVAAGHEVLVESGAGERSGFTDAEYEAVGARIAPVARQVFEQAELIVKVKEPQPP
ELAALRPGQILFTYLHLAPDPLQTKALLEHEVTAIAYETIRDASGRLPLLTPMSEVAGRMAAHVGAFYLQQPNGGRGMLL
GGVPGVPPAHVVVLGGGVVGLNAIKVAAGMGARVTVLDKSLPTLRYLDDIFGNRIETLWSNEAHVEQALVQADLVIGAVL
IPGAAAPRLVQRRMLKDMKPGCVIVDVAVDQGGCVETTRPTTHADPVYFVDGVLHYAVANMPGALPRTSTLALTNATLPY
AMQLAGRGVKDALLADPGFLAGLNTYRGKLTCAPAAESLGIPYTDPRTLL

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=352, Percent_Identity=31.5340909090909, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI122939153, Length=352, Percent_Identity=31.5340909090909, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787887, Length=341, Percent_Identity=32.8445747800587, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17550456, Length=356, Percent_Identity=30.8988764044944, Blast_Score=144, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38972; Mature: 38972

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGCPKEIKNNENRVGLTPGGARSLVAAGHEVLVESGAGERSGFTDAEYEAVGARIAPV
CCCCCCHHHCCCCCEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHCCHHHHH
ARQVFEQAELIVKVKEPQPPELAALRPGQILFTYLHLAPDPLQTKALLEHEVTAIAYETI
HHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RDASGRLPLLTPMSEVAGRMAAHVGAFYLQQPNGGRGMLLGGVPGVPPAHVVVLGGGVVG
HCCCCCCCEECCHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCEEEEECCCEEC
LNAIKVAAGMGARVTVLDKSLPTLRYLDDIFGNRIETLWSNEAHVEQALVQADLVIGAVL
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHC
IPGAAAPRLVQRRMLKDMKPGCVIVDVAVDQGGCVETTRPTTHADPVYFVDGVLHYAVAN
CCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHC
MPGALPRTSTLALTNATLPYAMQLAGRGVKDALLADPGFLAGLNTYRGKLTCAPAAESLG
CCCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHCCCCHHCCCCCCCCEEEECCCHHHCC
IPYTDPRTLL
CCCCCCCCCC
>Mature Secondary Structure
MRVGCPKEIKNNENRVGLTPGGARSLVAAGHEVLVESGAGERSGFTDAEYEAVGARIAPV
CCCCCCHHHCCCCCEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHCCHHHHH
ARQVFEQAELIVKVKEPQPPELAALRPGQILFTYLHLAPDPLQTKALLEHEVTAIAYETI
HHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RDASGRLPLLTPMSEVAGRMAAHVGAFYLQQPNGGRGMLLGGVPGVPPAHVVVLGGGVVG
HCCCCCCCEECCHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCEEEEECCCEEC
LNAIKVAAGMGARVTVLDKSLPTLRYLDDIFGNRIETLWSNEAHVEQALVQADLVIGAVL
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHC
IPGAAAPRLVQRRMLKDMKPGCVIVDVAVDQGGCVETTRPTTHADPVYFVDGVLHYAVAN
CCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHC
MPGALPRTSTLALTNATLPYAMQLAGRGVKDALLADPGFLAGLNTYRGKLTCAPAAESLG
CCCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHCCCCHHCCCCCCCCEEEECCCHHHCC
IPYTDPRTLL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2340274 [H]