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The map label for this gene is prfC [H]

Identifier: 220916948

GI number: 220916948

Start: 2063696

End: 2065321

Strand: Direct

Name: prfC [H]

Synonym: A2cp1_1844

Alternate gene names: 220916948

Gene position: 2063696-2065321 (Clockwise)

Preceding gene: 220916947

Following gene: 220916951

Centisome position: 41.03

GC content: 71.16

Gene sequence:

>1626_bases
ATGAGCCACCACGAGATCTCGCGCCGGCGCACCTTCGCCATCATCGCCCACCCGGACGCGGGCAAGACCACGCTCACCGA
GAAGCTGCTGCTCTACGGCGGCGTCATCCAGCTCGCCGGCGCGGTGAAGGCGAAGCGCGGCCGAGCCAACGCGGTCTCGG
ACTGGATGGAGATGGAGCGCGAGCGCGGCATCTCCATCACCACCAGCGTCCTGCAGTTCCCCTACCGCGGGCTGCAGATG
AACCTGCTCGACACCCCCGGCCACGCCGACTTCAGCGAGGACACTTACCGCACGCTGCACGCGGTGGACGGGGCGGTCAT
GCTGCTCGACTGCGCCAAGGGCGTCGAGTCGCAGACCCGCAAGCTGTTCCGCGTCTGCCGGCAGCGCTCCATCCCCATCT
TCACGTTCGTGAACAAGATGGACCGCCCGGGCCGCGACGCGTTCGAGCTCATCGGCGAGGTGGAGAGCGTCCTCGGCATC
GGCGTCTACCCCATCACCTGGCCGGTGTTCCGCAGCGGCGTGTTCCGCGGCGTCTACCACCGCATGGCGCGGCGCGTGTA
CCTGTTCGACGCCGACCACGCCAACTCCAGCTCCACCACCGGCGCCGAGCGGCCGCCGGTGGAGGTGACCGGCATCGACG
ACCCGATGCTGCGCGAGGCGCTCGACGACGCCGGCTACGATCGCCTGCGCTCCGAGGCCGACCTGCTCGACGCCGCCGGC
GACGGCTTCGACCGCGAGCGCTTCGAGGCGGGCGAGCTGTCGCCCATGTTCTTCGGCAGCGCGGTGAACAACTTCGGCCT
GGAGGCGTTCCTCGAGACGTTCAGCGAGCTGATGCCGCCGCCGCGCCCCCGCGACACCGACCAGGGCCCGGTGGAGCCGA
CCCGCGACGAGTTCAGCGGGTTCGTCTTCAAGATCCAGGCGAACATGGACAAGGCACACCGCGACCGCGTCGCGTTCGTG
CGCATCTGCTCGGGCCGGATGGTCCGCGGGATGAAGGCGCACCACGTCCGCACCGGCAAGGACGTCCGGCTCGCCAACCC
CACCCAGTTCCTGGCCCGCGACCGCAACGTGGTGGACGAGTCCTGGGCCGGCGACGTGGTGGGCATCCACGACCCCGGCA
ACCTGGAGATCGGCGACACGCTCACCGGCGGCTCGCGCTTCGTCTACGAGGGCATCCCCAGCTTCGCGCCGGAGCACTTC
CGGCGGCTCGCGCTGGTGGACCCCATGCGCCGCAAGCAGTTCGCGACCGGGCTCGAGCAGCTCGCGCAGGAGGGTACGGT
GCAGCTCTACCGGCCGCCGGCCGGCCGCGCCGGTGATCTCGTGCTGGGCGCCCTCGGCCAGCTCCAGTTCGAGGTGGTGC
GCTACCGCCTCGAGTCGGAGTACTCGGTGCAGGTGCGCGTGGAACCGGTGCCCTACCAGCTCGCGCGCTGGGTCTCGCGC
GCCGACGGCAAGCCGCTCGATCTCGACGCGCTCCACGACGCGGTCGAGGGCATGGTGGTGCTCGACGTGCGCGACCGGCC
GGTGGTGCTGTTCGACCGCGAGTGGGCGCTCCGCACCGCGGAGCGGCTCCACCCCGAGTACCGGTTCGCCGAGACCGCCA
CCGGCGTGGTGGTCCGGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGCCGCTGGGTTAGAGTGCGCCCCCGACGACACAGACGCTCGCTCGCCCCGGGTCCCGCGGCCCGGGGCGATTCGCATTC
TGGAAGGACCCCCGCAGTCC

Downstream 100 bases:

>100_bases
CCCCCCGCCCGCTCGCCCTTCGACAGGCTCAGGGCGAGCGGAGGTGAGGGAATCCGCGCTCAGCTCCCGCGCTGGATGAG
CTCGACCTTGTAGCCGTCGG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSHHEISRRRTFAIIAHPDAGKTTLTEKLLLYGGVIQLAGAVKAKRGRANAVSDWMEMERERGISITTSVLQFPYRGLQM
NLLDTPGHADFSEDTYRTLHAVDGAVMLLDCAKGVESQTRKLFRVCRQRSIPIFTFVNKMDRPGRDAFELIGEVESVLGI
GVYPITWPVFRSGVFRGVYHRMARRVYLFDADHANSSSTTGAERPPVEVTGIDDPMLREALDDAGYDRLRSEADLLDAAG
DGFDRERFEAGELSPMFFGSAVNNFGLEAFLETFSELMPPPRPRDTDQGPVEPTRDEFSGFVFKIQANMDKAHRDRVAFV
RICSGRMVRGMKAHHVRTGKDVRLANPTQFLARDRNVVDESWAGDVVGIHDPGNLEIGDTLTGGSRFVYEGIPSFAPEHF
RRLALVDPMRRKQFATGLEQLAQEGTVQLYRPPAGRAGDLVLGALGQLQFEVVRYRLESEYSVQVRVEPVPYQLARWVSR
ADGKPLDLDALHDAVEGMVVLDVRDRPVVLFDREWALRTAERLHPEYRFAETATGVVVRAA

Sequences:

>Translated_541_residues
MSHHEISRRRTFAIIAHPDAGKTTLTEKLLLYGGVIQLAGAVKAKRGRANAVSDWMEMERERGISITTSVLQFPYRGLQM
NLLDTPGHADFSEDTYRTLHAVDGAVMLLDCAKGVESQTRKLFRVCRQRSIPIFTFVNKMDRPGRDAFELIGEVESVLGI
GVYPITWPVFRSGVFRGVYHRMARRVYLFDADHANSSSTTGAERPPVEVTGIDDPMLREALDDAGYDRLRSEADLLDAAG
DGFDRERFEAGELSPMFFGSAVNNFGLEAFLETFSELMPPPRPRDTDQGPVEPTRDEFSGFVFKIQANMDKAHRDRVAFV
RICSGRMVRGMKAHHVRTGKDVRLANPTQFLARDRNVVDESWAGDVVGIHDPGNLEIGDTLTGGSRFVYEGIPSFAPEHF
RRLALVDPMRRKQFATGLEQLAQEGTVQLYRPPAGRAGDLVLGALGQLQFEVVRYRLESEYSVQVRVEPVPYQLARWVSR
ADGKPLDLDALHDAVEGMVVLDVRDRPVVLFDREWALRTAERLHPEYRFAETATGVVVRAA
>Mature_540_residues
SHHEISRRRTFAIIAHPDAGKTTLTEKLLLYGGVIQLAGAVKAKRGRANAVSDWMEMERERGISITTSVLQFPYRGLQMN
LLDTPGHADFSEDTYRTLHAVDGAVMLLDCAKGVESQTRKLFRVCRQRSIPIFTFVNKMDRPGRDAFELIGEVESVLGIG
VYPITWPVFRSGVFRGVYHRMARRVYLFDADHANSSSTTGAERPPVEVTGIDDPMLREALDDAGYDRLRSEADLLDAAGD
GFDRERFEAGELSPMFFGSAVNNFGLEAFLETFSELMPPPRPRDTDQGPVEPTRDEFSGFVFKIQANMDKAHRDRVAFVR
ICSGRMVRGMKAHHVRTGKDVRLANPTQFLARDRNVVDESWAGDVVGIHDPGNLEIGDTLTGGSRFVYEGIPSFAPEHFR
RLALVDPMRRKQFATGLEQLAQEGTVQLYRPPAGRAGDLVLGALGQLQFEVVRYRLESEYSVQVRVEPVPYQLARWVSRA
DGKPLDLDALHDAVEGMVVLDVRDRPVVLFDREWALRTAERLHPEYRFAETATGVVVRAA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=486, Percent_Identity=27.7777777777778, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI19923640, Length=548, Percent_Identity=24.6350364963504, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI25306283, Length=359, Percent_Identity=29.2479108635098, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI25306287, Length=299, Percent_Identity=31.438127090301, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI157426893, Length=164, Percent_Identity=34.1463414634146, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=32.8859060402685, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=32.3741007194245, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI310132016, Length=118, Percent_Identity=33.8983050847458, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=118, Percent_Identity=33.8983050847458, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=118, Percent_Identity=33.8983050847458, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=48.7712665406427, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1789738, Length=489, Percent_Identity=25.7668711656442, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI48994988, Length=139, Percent_Identity=35.2517985611511, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=494, Percent_Identity=24.6963562753036, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=181, Percent_Identity=34.8066298342541, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=149, Percent_Identity=37.5838926174497, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=34.1614906832298, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=32.5925925925926, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=32.5925925925926, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=525, Percent_Identity=26.4761904761905, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322359, Length=517, Percent_Identity=25.3384912959381, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=154, Percent_Identity=30.5194805194805, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=154, Percent_Identity=30.5194805194805, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324166, Length=149, Percent_Identity=33.5570469798658, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=493, Percent_Identity=25.3549695740365, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI221458488, Length=512, Percent_Identity=27.5390625, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI78706572, Length=163, Percent_Identity=35.5828220858896, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=34.640522875817, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=34.640522875817, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=34.640522875817, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=31.9444444444444, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357743, Length=164, Percent_Identity=32.3170731707317, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60650; Mature: 60518

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHHEISRRRTFAIIAHPDAGKTTLTEKLLLYGGVIQLAGAVKAKRGRANAVSDWMEMER
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ERGISITTSVLQFPYRGLQMNLLDTPGHADFSEDTYRTLHAVDGAVMLLDCAKGVESQTR
HCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHCCHHHHHH
KLFRVCRQRSIPIFTFVNKMDRPGRDAFELIGEVESVLGIGVYPITWPVFRSGVFRGVYH
HHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHH
RMARRVYLFDADHANSSSTTGAERPPVEVTGIDDPMLREALDDAGYDRLRSEADLLDAAG
HHHCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
DGFDRERFEAGELSPMFFGSAVNNFGLEAFLETFSELMPPPRPRDTDQGPVEPTRDEFSG
CCCCHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCC
FVFKIQANMDKAHRDRVAFVRICSGRMVRGMKAHHVRTGKDVRLANPTQFLARDRNVVDE
EEEEEEECCCHHHHHHHHHHHHHCCHHHCCCHHHCCCCCCCEEECCHHHHHHHCCCCCCC
SWAGDVVGIHDPGNLEIGDTLTGGSRFVYEGIPSFAPEHFRRLALVDPMRRKQFATGLEQ
CCCCCEEEECCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
LAQEGTVQLYRPPAGRAGDLVLGALGQLQFEVVRYRLESEYSVQVRVEPVPYQLARWVSR
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHH
ADGKPLDLDALHDAVEGMVVLDVRDRPVVLFDREWALRTAERLHPEYRFAETATGVVVRA
CCCCCCCHHHHHHHHCCEEEEEECCCCEEEECCCHHHHHHHHCCCCHHHHHHCCCEEEEE
A
C
>Mature Secondary Structure 
SHHEISRRRTFAIIAHPDAGKTTLTEKLLLYGGVIQLAGAVKAKRGRANAVSDWMEMER
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ERGISITTSVLQFPYRGLQMNLLDTPGHADFSEDTYRTLHAVDGAVMLLDCAKGVESQTR
HCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHCCHHHHHH
KLFRVCRQRSIPIFTFVNKMDRPGRDAFELIGEVESVLGIGVYPITWPVFRSGVFRGVYH
HHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHH
RMARRVYLFDADHANSSSTTGAERPPVEVTGIDDPMLREALDDAGYDRLRSEADLLDAAG
HHHCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
DGFDRERFEAGELSPMFFGSAVNNFGLEAFLETFSELMPPPRPRDTDQGPVEPTRDEFSG
CCCCHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCC
FVFKIQANMDKAHRDRVAFVRICSGRMVRGMKAHHVRTGKDVRLANPTQFLARDRNVVDE
EEEEEEECCCHHHHHHHHHHHHHCCHHHCCCHHHCCCCCCCEEECCHHHHHHHCCCCCCC
SWAGDVVGIHDPGNLEIGDTLTGGSRFVYEGIPSFAPEHFRRLALVDPMRRKQFATGLEQ
CCCCCEEEECCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
LAQEGTVQLYRPPAGRAGDLVLGALGQLQFEVVRYRLESEYSVQVRVEPVPYQLARWVSR
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHH
ADGKPLDLDALHDAVEGMVVLDVRDRPVVLFDREWALRTAERLHPEYRFAETATGVVVRA
CCCCCCCHHHHHHHHCCEEEEEECCCCEEEECCCHHHHHHHHCCCCHHHHHHCCCEEEEE
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA