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The map label for this gene is greB [H]

Identifier: 220916942

GI number: 220916942

Start: 2057204

End: 2057710

Strand: Direct

Name: greB [H]

Synonym: A2cp1_1838

Alternate gene names: 220916942

Gene position: 2057204-2057710 (Clockwise)

Preceding gene: 220916940

Following gene: 220916947

Centisome position: 40.9

GC content: 80.28

Gene sequence:

>507_bases
GTGTCCAAGGCCTTCACCAGCGAGGAGACGCCCGACCTCGGCCCGGTGGTGCGCCCGCCGCCGCGGCTCGCGCCGGGCGA
GGTGCGCTATGTCACGCCCGAGGGGCAGGCCGCGCTGCGCGAGGCGCTGGCGCGGCTCCGGGCCGAGCGCGCCGACGCGG
CCCGGCTCCCAGACGCCGAGCGCGCGCCGCGGCTCGCCGACCTGGACGCGCGCATCGCAGCGCTCGACGCGACGCTGGGC
GCGCTCACCGTGCTCGGGCCCGAGGCGACGCCGGAGGGGCAGGTGGGGTTCGGGACCTGGGTGACCGTCGAGGAGGAGGA
CGGCCGCCGGGTCACCTGGCGCCTGGTCGGGCCGGACGAGGCCGACGCGCGGCGCGGGCTCATCAGCGCCGGCTCGCCGG
TGGCACGCGCGCTGCTCGGGCGTCGCGCCGGGGAGGTGGTGGAGGTGCACCGGCCCGGCGGCGAGGCGGAGCTGACCGTG
GTGGCGGTGCGGCGCACCGCGCCCTGA

Upstream 100 bases:

>100_bases
CCGCGCAAGCTGCGCCGAAGCTGCGCCACCGTGTCGCACGGCCCGGCCCGGGCGCCCGGCCCGGCGGGCGCGCCCGGCGG
CCGGCCGCTATCCTCGGAGC

Downstream 100 bases:

>100_bases
CGCGACGCTCGCCCTTCGACAGGCTCAGGGCGAGCGGAAGACCCCGGTCGCTCATCCCGACCCTTCGACTCCGTTCGCTC
ATCCCGACCCTTCGACTCCG

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSKAFTSEETPDLGPVVRPPPRLAPGEVRYVTPEGQAALREALARLRAERADAARLPDAERAPRLADLDARIAALDATLG
ALTVLGPEATPEGQVGFGTWVTVEEEDGRRVTWRLVGPDEADARRGLISAGSPVARALLGRRAGEVVEVHRPGGEAELTV
VAVRRTAP

Sequences:

>Translated_168_residues
MSKAFTSEETPDLGPVVRPPPRLAPGEVRYVTPEGQAALREALARLRAERADAARLPDAERAPRLADLDARIAALDATLG
ALTVLGPEATPEGQVGFGTWVTVEEEDGRRVTWRLVGPDEADARRGLISAGSPVARALLGRRAGEVVEVHRPGGEAELTV
VAVRRTAP
>Mature_167_residues
SKAFTSEETPDLGPVVRPPPRLAPGEVRYVTPEGQAALREALARLRAERADAARLPDAERAPRLADLDARIAALDATLGA
LTVLGPEATPEGQVGFGTWVTVEEEDGRRVTWRLVGPDEADARRGLISAGSPVARALLGRRAGEVVEVHRPGGEAELTVV
AVRRTAP

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=37.2549019607843, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI87082225, Length=98, Percent_Identity=40.8163265306122, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17823; Mature: 17692

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAFTSEETPDLGPVVRPPPRLAPGEVRYVTPEGQAALREALARLRAERADAARLPDAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHCCCCCC
RAPRLADLDARIAALDATLGALTVLGPEATPEGQVGFGTWVTVEEEDGRRVTWRLVGPDE
CCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCC
ADARRGLISAGSPVARALLGRRAGEVVEVHRPGGEAELTVVAVRRTAP
CHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
SKAFTSEETPDLGPVVRPPPRLAPGEVRYVTPEGQAALREALARLRAERADAARLPDAE
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHCCCCCC
RAPRLADLDARIAALDATLGALTVLGPEATPEGQVGFGTWVTVEEEDGRRVTWRLVGPDE
CCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCC
ADARRGLISAGSPVARALLGRRAGEVVEVHRPGGEAELTVVAVRRTAP
CHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]