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The map label for this gene is gcvH [H]

Identifier: 220916747

GI number: 220916747

Start: 1832950

End: 1833696

Strand: Direct

Name: gcvH [H]

Synonym: A2cp1_1642

Alternate gene names: 220916747

Gene position: 1832950-1833696 (Clockwise)

Preceding gene: 220916746

Following gene: 220916749

Centisome position: 36.45

GC content: 75.64

Gene sequence:

>747_bases
ATGGCCTACGATCTACTTTCCGTGTACCCGGCGAAGCTCCTGGAGTACGGCCTCGGGATCGGCTACCTGCTGCTCTTCAT
CCCCTTCTGGAGGTACGTGCAGGGCGGCCGGCGCGAGGCGCGCGAGGTGATGCGGGTCGCGGTGCCGGTGCGCGAGGCGG
TGTCCGCCGCCCTCCGTGCGGCGCGTCCCGCGGGCGCGACCGCCATCGCCCGGCCGGCCTCGGCCGGCTGGTTCCACGTC
CCGGCCGACGTGCACCTGCACCCCGGCCACACCTGGGCGCGGCTGGGCGGCGACGGGCTCGTCTCCGTGGGCATCGACGA
CTTCGCCCACAAGCTGGTGGGGCCGGCGACGGTGGAGCTGCCCGCGGTCGGGGCGCGGGTGGCGCAGGGCGAGCCCGCCA
TCGAGCTGCGCGAAGAGGACCGCGGGGTCGCGATGCTCTCGCCGGTGGACGGCACCGTGGTCGCGGTGAACCCGCGCGTC
CGCGCCCACGGCGCGCTCGAGGACCCCTACGGCGCGGGCTGGCTGTTCAAGGTGAAGGCGCCGCGGCTGGTGGCGAACTT
CCGGCAGCTCCAGGCCGAGGCGCCGGCGCAGCGGATGCTCGACGTGGCCGGGGACACGCTGGCGCTCCGCCTCGCGCCCG
AGCTCGGGCAGGTCCTGCAGGACGGCGGCACGCCGGTCCACGGCATCGCCCGCGCGCTGGCCGGGGAGGGCTGGGACGAG
CTGGCGCGGGAGTACTTCCTGACCTGA

Upstream 100 bases:

>100_bases
TGCTGGCCCTCATCGGATTCGCCATCATCCCGGCCCTGAACCTCTCCGCCTCGCACTGACTCCCGTCGGCGGCAGCGCAC
GCGTACGGAGCGAGGAGCCC

Downstream 100 bases:

>100_bases
GCCGCCGCGGACGCACGCGAGGGGCCGGCGAGCGCCGGCCCTTCGCCGTCGGACGCGGTCCTCATCGCCGGCGGCGGCCG
GCGACCCGGCGCGCGGGCGC

Product: glycine cleavage H-protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MAYDLLSVYPAKLLEYGLGIGYLLLFIPFWRYVQGGRREAREVMRVAVPVREAVSAALRAARPAGATAIARPASAGWFHV
PADVHLHPGHTWARLGGDGLVSVGIDDFAHKLVGPATVELPAVGARVAQGEPAIELREEDRGVAMLSPVDGTVVAVNPRV
RAHGALEDPYGAGWLFKVKAPRLVANFRQLQAEAPAQRMLDVAGDTLALRLAPELGQVLQDGGTPVHGIARALAGEGWDE
LAREYFLT

Sequences:

>Translated_248_residues
MAYDLLSVYPAKLLEYGLGIGYLLLFIPFWRYVQGGRREAREVMRVAVPVREAVSAALRAARPAGATAIARPASAGWFHV
PADVHLHPGHTWARLGGDGLVSVGIDDFAHKLVGPATVELPAVGARVAQGEPAIELREEDRGVAMLSPVDGTVVAVNPRV
RAHGALEDPYGAGWLFKVKAPRLVANFRQLQAEAPAQRMLDVAGDTLALRLAPELGQVLQDGGTPVHGIARALAGEGWDE
LAREYFLT
>Mature_247_residues
AYDLLSVYPAKLLEYGLGIGYLLLFIPFWRYVQGGRREAREVMRVAVPVREAVSAALRAARPAGATAIARPASAGWFHVP
ADVHLHPGHTWARLGGDGLVSVGIDDFAHKLVGPATVELPAVGARVAQGEPAIELREEDRGVAMLSPVDGTVVAVNPRVR
AHGALEDPYGAGWLFKVKAPRLVANFRQLQAEAPAQRMLDVAGDTLALRLAPELGQVLQDGGTPVHGIARALAGEGWDEL
AREYFLT

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789271, Length=105, Percent_Identity=36.1904761904762, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 26558; Mature: 26427

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDLLSVYPAKLLEYGLGIGYLLLFIPFWRYVQGGRREAREVMRVAVPVREAVSAALRA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ARPAGATAIARPASAGWFHVPADVHLHPGHTWARLGGDGLVSVGIDDFAHKLVGPATVEL
CCCCCCHHHCCCCCCCEEECCCCEEECCCCCEEEECCCCEEEECHHHHHHHHCCCCEEEC
PAVGARVAQGEPAIELREEDRGVAMLSPVDGTVVAVNPRVRAHGALEDPYGAGWLFKVKA
CCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEECCCEEECCCCCCCCCCCEEEEECC
PRLVANFRQLQAEAPAQRMLDVAGDTLALRLAPELGQVLQDGGTPVHGIARALAGEGWDE
CHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCHHHHHHHHCCCCHHH
LAREYFLT
HHHHHCCC
>Mature Secondary Structure 
AYDLLSVYPAKLLEYGLGIGYLLLFIPFWRYVQGGRREAREVMRVAVPVREAVSAALRA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ARPAGATAIARPASAGWFHVPADVHLHPGHTWARLGGDGLVSVGIDDFAHKLVGPATVEL
CCCCCCHHHCCCCCCCEEECCCCEEECCCCCEEEECCCCEEEECHHHHHHHHCCCCEEEC
PAVGARVAQGEPAIELREEDRGVAMLSPVDGTVVAVNPRVRAHGALEDPYGAGWLFKVKA
CCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEECCCEEECCCCCCCCCCCEEEEECC
PRLVANFRQLQAEAPAQRMLDVAGDTLALRLAPELGQVLQDGGTPVHGIARALAGEGWDE
CHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCHHHHHHHHCCCCHHH
LAREYFLT
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA