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The map label for this gene is rpfG [H]

Identifier: 220916371

GI number: 220916371

Start: 1416783

End: 1417910

Strand: Reverse

Name: rpfG [H]

Synonym: A2cp1_1265

Alternate gene names: 220916371

Gene position: 1417910-1416783 (Counterclockwise)

Preceding gene: 220916372

Following gene: 220916369

Centisome position: 28.19

GC content: 71.72

Gene sequence:

>1128_bases
ATGAACGCGGACCGGGTCTTCATCGTCGACGACGACGAGCTGATCCTGAAGGCGCTCGCGCGCGTGCTGGAGCTTTCGGG
GTACGAGACCCGCTGCTTCCTCCGGCCGGCCGACGCGCTCGCCGCCATCGAGGCCGAGGCCCCGGTGGTGGTGATCTCCG
ACTACATGATGCCGAGCATGGACGGCGTCGCCTTCCTCAAGGCGGCGCGCGCCCGCTTCCCCGCCGCCGTCCGCATCCTC
TGCACCGCCGCCGAGGACTTTCGCGTGGCGCTGCAGGCCGTGAACGCGGGCGAGGTCTTCCGGATCATCTCGAAGCCGTG
GCACCAGCAGGAGCTGCTCACCACGGTGAGCCAGGCCGCCGAGGCGGCGCGGCTGCGCATCGAGAACGAGCGCCTCACCG
CCGAGGTGCACCGGCAGAACGGGCAGCTGAAGGAGATCAACACCCGGCTCGAGCAGATGGTACAGCAGCGCACCCAGGCG
CTGCTGGAGGGGCTCATCGCCGCGCTCGACTACCGGGACGCCGAGACCCAGTGGCACTCGCGGCGCGTCTCGCTCTACGC
GCGGCGCCTGGCGCACCAGCTCGGCATGGAGGAGCCGGCGCTCACCGTGATCGAGCACGGCGCCCTGCTGCACGACATCG
GCAAGATCGGCGTGCGCGACCGCGTGCTGCTGAAGCCGGGGCCGCTCACCGGCGAGGAGTGGACCGAGATGAAGCGCCAC
CCGGAGCTCGGGTGGGCGCTCCTGCAGCGGGTGGACTACCTGCGGCCCGCCTCGGCGATCGTCCTGCAGCACCAGGAGAA
GTGGGACGGCAGCGGGTATCCCTCCGGGCTGGGCGGAGAGGAGATCGTCATCGGCGCGCGCATCTTCCACGTGGTGGACA
CGCTCGACGCCATCACCAGCGATCGGCCCTACCGCCGCTCGCGCCCGTTCGCGGAGGCGCGCGAGGAGATCGTCCGCTGC
CGCGGGACGCAGTTCGATCCGCGCGTGGTGGACGCGTTCGTGGCGGTGCCGCCGGAGGACTGGGAGCGCATCCGGCTCGA
CGTCGAGACGGTGGCGGTGCTCTCCGCCGACCTGGCCGAGAGCCCGCCGCACCTCGAGGACCTGGATCTCGCCGGCGCGC
GCGCCTGA

Upstream 100 bases:

>100_bases
CGCACACGCCGGGCCGTGCGGCGCTGGATCGCGGCCGGTAGAATCGGCCCCCATGGATCCGCACCTTGCCCCGCCCGCCC
CGCCCTGCTCTACTCGCGCG

Downstream 100 bases:

>100_bases
GCGGCGCCGCTACTCCAGCGCCACGCCGAGCAGCTCCGCGGTCTCGCCCGCGCGCACCTCGACCGTCGCGCCGGCGCGGA
AGCCGTCCTCCGTCCGGAAC

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNADRVFIVDDDELILKALARVLELSGYETRCFLRPADALAAIEAEAPVVVISDYMMPSMDGVAFLKAARARFPAAVRIL
CTAAEDFRVALQAVNAGEVFRIISKPWHQQELLTTVSQAAEAARLRIENERLTAEVHRQNGQLKEINTRLEQMVQQRTQA
LLEGLIAALDYRDAETQWHSRRVSLYARRLAHQLGMEEPALTVIEHGALLHDIGKIGVRDRVLLKPGPLTGEEWTEMKRH
PELGWALLQRVDYLRPASAIVLQHQEKWDGSGYPSGLGGEEIVIGARIFHVVDTLDAITSDRPYRRSRPFAEAREEIVRC
RGTQFDPRVVDAFVAVPPEDWERIRLDVETVAVLSADLAESPPHLEDLDLAGARA

Sequences:

>Translated_375_residues
MNADRVFIVDDDELILKALARVLELSGYETRCFLRPADALAAIEAEAPVVVISDYMMPSMDGVAFLKAARARFPAAVRIL
CTAAEDFRVALQAVNAGEVFRIISKPWHQQELLTTVSQAAEAARLRIENERLTAEVHRQNGQLKEINTRLEQMVQQRTQA
LLEGLIAALDYRDAETQWHSRRVSLYARRLAHQLGMEEPALTVIEHGALLHDIGKIGVRDRVLLKPGPLTGEEWTEMKRH
PELGWALLQRVDYLRPASAIVLQHQEKWDGSGYPSGLGGEEIVIGARIFHVVDTLDAITSDRPYRRSRPFAEAREEIVRC
RGTQFDPRVVDAFVAVPPEDWERIRLDVETVAVLSADLAESPPHLEDLDLAGARA
>Mature_375_residues
MNADRVFIVDDDELILKALARVLELSGYETRCFLRPADALAAIEAEAPVVVISDYMMPSMDGVAFLKAARARFPAAVRIL
CTAAEDFRVALQAVNAGEVFRIISKPWHQQELLTTVSQAAEAARLRIENERLTAEVHRQNGQLKEINTRLEQMVQQRTQA
LLEGLIAALDYRDAETQWHSRRVSLYARRLAHQLGMEEPALTVIEHGALLHDIGKIGVRDRVLLKPGPLTGEEWTEMKRH
PELGWALLQRVDYLRPASAIVLQHQEKWDGSGYPSGLGGEEIVIGARIFHVVDTLDAITSDRPYRRSRPFAEAREEIVRC
RGTQFDPRVVDAFVAVPPEDWERIRLDVETVAVLSADLAESPPHLEDLDLAGARA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=35.9649122807018, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=125, Percent_Identity=32, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 42052; Mature: 42052

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNADRVFIVDDDELILKALARVLELSGYETRCFLRPADALAAIEAEAPVVVISDYMMPSM
CCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCEEEEECCCCCCC
DGVAFLKAARARFPAAVRILCTAAEDFRVALQAVNAGEVFRIISKPWHQQELLTTVSQAA
CHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHH
EAARLRIENERLTAEVHRQNGQLKEINTRLEQMVQQRTQALLEGLIAALDYRDAETQWHS
HHHHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RRVSLYARRLAHQLGMEEPALTVIEHGALLHDIGKIGVRDRVLLKPGPLTGEEWTEMKRH
HHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCC
PELGWALLQRVDYLRPASAIVLQHQEKWDGSGYPSGLGGEEIVIGARIFHVVDTLDAITS
CCHHHHHHHHHHHCCCCHHHHEECHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHC
DRPYRRSRPFAEAREEIVRCRGTQFDPRVVDAFVAVPPEDWERIRLDVETVAVLSADLAE
CCCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHC
SPPHLEDLDLAGARA
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MNADRVFIVDDDELILKALARVLELSGYETRCFLRPADALAAIEAEAPVVVISDYMMPSM
CCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCEEEEECCCCCCC
DGVAFLKAARARFPAAVRILCTAAEDFRVALQAVNAGEVFRIISKPWHQQELLTTVSQAA
CHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHH
EAARLRIENERLTAEVHRQNGQLKEINTRLEQMVQQRTQALLEGLIAALDYRDAETQWHS
HHHHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RRVSLYARRLAHQLGMEEPALTVIEHGALLHDIGKIGVRDRVLLKPGPLTGEEWTEMKRH
HHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCC
PELGWALLQRVDYLRPASAIVLQHQEKWDGSGYPSGLGGEEIVIGARIFHVVDTLDAITS
CCHHHHHHHHHHHCCCCHHHHEECHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHC
DRPYRRSRPFAEAREEIVRCRGTQFDPRVVDAFVAVPPEDWERIRLDVETVAVLSADLAE
CCCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHC
SPPHLEDLDLAGARA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]