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The map label for this gene is tolB

Identifier: 220915811

GI number: 220915811

Start: 778726

End: 780030

Strand: Reverse

Name: tolB

Synonym: A2cp1_0694

Alternate gene names: 220915811

Gene position: 780030-778726 (Counterclockwise)

Preceding gene: 220915812

Following gene: 220915810

Centisome position: 15.51

GC content: 74.64

Gene sequence:

>1305_bases
ATGACCGTCCGCCGTGCCCTCGCCCTCGCCGCGCTCGCGCTGGCCGTCTCCCCCGCGCTCGCCGCGCAGGAGCGCCCCAC
CATCGTGGTCGGCTCGCCCGACTTCCGCCCGCTGCCCATCGCGGTGGCGGCGTTCCAGGGCGAGGGCGACGCCGGCGCCG
CCGCGACCCAGACCGCGGAGGTGGTGCGCGCCGACCTGGTGCTGTCGGGCCTGTTCGACGTGCTCGACCCGCGGGGCTTC
CTCGCCGATCCGTCGGAGGGCTTCGCGGCGCCGTCGATCCGGTTCGCGCGCTGGGCCGACGTGGGCGCGGACGGCCTCGC
CAAGGCGCGCGTGCGCCGCGGACCGGCCGGCCTGGAGGGCGAGCTCCACCTGTACGAGGTCCGCGCCGGGCGCGAGGTGC
TGGTGAAGCTGCTGCGCGTGGACGGCGCCGACGCGCGCTCGCTCGCCCACCGGATGGCCGACGAGATCGTGCGCTACTAC
ACGCGCGAGCCCGGCATCTTCGCGACGCGCATCGCCGCGATCCGCCGCGGCCGCGGCACCTGGGAGCTCGTGACGCAGGA
CATGGACGGCGGGAACCAGCAGGTCCTGCTGTCCGAGCGGAGCATCCTCATGTCGCCGGCGTGGCGCCCGGACGGCCGCG
AGATCCTGGTCACCAGCTACCGGTCCGGCCGGCCGGAGCTGTGGGCCTACCGCTTCTCCGACCGCGCGTTCCGCCCGCTG
GGCCGCCACCGCAACGCGTTCGGCGGCGTGTACTCGCCCGACGGCTCCCGCATCGCGTTCACCGTCAGCGAAGGCAACGT
CACCGATCTCTGGGTGATGTCCGCCGACGGCGCCGGCGCGCGCAAGCTCACCAGCGACCCCGCCATCGACGTGTCGCCCA
CCTGGTCGCCCGACGGCCGCCGCATCGCGTTCGTCTCCGATCGCTCCGGCACCCCGCAGATCTACGTGATGGGCGCGGAC
GGCTCGGGCGCGCGGCGCCTGACGTTCCAGGGCAACTACAACCAGACGCCGCAGTGGAGCCCTCGCGGCGACCTGATCGC
GTTCACCGCGCGCGACGAGCGCAAGGTGTTCGACGTGTTCGTGGTGTCGCCGGACTCGGGCGCCATCAACCGCATCACCC
AGGACCAGGGGCGCACCAACGAGGAGCCGTCCTGGGCGCCCAACGGCCGGCTCATGATCTTCCGCACCGACCGGAACGGC
GGGATCCAGCTGGTCGTGTCGGACGCGCGCGGCGACCGCCAGACGCCGGTGACGAGCGGCAAGACCGACCTGGCCGCGCC
GGCCTGGGGCCCGCTGGCGCCGTAG

Upstream 100 bases:

>100_bases
AGACCCGCCTCCCCCCTCCGCCCGACGCGCAGCGGCAGCTGTACCGCTCGACCGGGCTCGCCATCGTCTTCGAGATCGGC
TGATCTCCCGGAGCCCCGCC

Downstream 100 bases:

>100_bases
GCGGGGACGGCATGGCCGGGCGGCTCGCCTCGATCGACGTCGGCACCAACACGGTGCTGCTGCTCGTGGCGGAGCGGCGC
GGCGACGCGCTCGCGCCGGT

Product: TolB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTVRRALALAALALAVSPALAAQERPTIVVGSPDFRPLPIAVAAFQGEGDAGAAATQTAEVVRADLVLSGLFDVLDPRGF
LADPSEGFAAPSIRFARWADVGADGLAKARVRRGPAGLEGELHLYEVRAGREVLVKLLRVDGADARSLAHRMADEIVRYY
TREPGIFATRIAAIRRGRGTWELVTQDMDGGNQQVLLSERSILMSPAWRPDGREILVTSYRSGRPELWAYRFSDRAFRPL
GRHRNAFGGVYSPDGSRIAFTVSEGNVTDLWVMSADGAGARKLTSDPAIDVSPTWSPDGRRIAFVSDRSGTPQIYVMGAD
GSGARRLTFQGNYNQTPQWSPRGDLIAFTARDERKVFDVFVVSPDSGAINRITQDQGRTNEEPSWAPNGRLMIFRTDRNG
GIQLVVSDARGDRQTPVTSGKTDLAAPAWGPLAP

Sequences:

>Translated_434_residues
MTVRRALALAALALAVSPALAAQERPTIVVGSPDFRPLPIAVAAFQGEGDAGAAATQTAEVVRADLVLSGLFDVLDPRGF
LADPSEGFAAPSIRFARWADVGADGLAKARVRRGPAGLEGELHLYEVRAGREVLVKLLRVDGADARSLAHRMADEIVRYY
TREPGIFATRIAAIRRGRGTWELVTQDMDGGNQQVLLSERSILMSPAWRPDGREILVTSYRSGRPELWAYRFSDRAFRPL
GRHRNAFGGVYSPDGSRIAFTVSEGNVTDLWVMSADGAGARKLTSDPAIDVSPTWSPDGRRIAFVSDRSGTPQIYVMGAD
GSGARRLTFQGNYNQTPQWSPRGDLIAFTARDERKVFDVFVVSPDSGAINRITQDQGRTNEEPSWAPNGRLMIFRTDRNG
GIQLVVSDARGDRQTPVTSGKTDLAAPAWGPLAP
>Mature_433_residues
TVRRALALAALALAVSPALAAQERPTIVVGSPDFRPLPIAVAAFQGEGDAGAAATQTAEVVRADLVLSGLFDVLDPRGFL
ADPSEGFAAPSIRFARWADVGADGLAKARVRRGPAGLEGELHLYEVRAGREVLVKLLRVDGADARSLAHRMADEIVRYYT
REPGIFATRIAAIRRGRGTWELVTQDMDGGNQQVLLSERSILMSPAWRPDGREILVTSYRSGRPELWAYRFSDRAFRPLG
RHRNAFGGVYSPDGSRIAFTVSEGNVTDLWVMSADGAGARKLTSDPAIDVSPTWSPDGRRIAFVSDRSGTPQIYVMGADG
SGARRLTFQGNYNQTPQWSPRGDLIAFTARDERKVFDVFVVSPDSGAINRITQDQGRTNEEPSWAPNGRLMIFRTDRNGG
IQLVVSDARGDRQTPVTSGKTDLAAPAWGPLAP

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=406, Percent_Identity=31.0344827586207, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_ANAD2 (B8JD21)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491115.1
- ProteinModelPortal:   B8JD21
- SMR:   B8JD21
- GeneID:   7300416
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0694
- HOGENOM:   HBG644919
- ProtClustDB:   CLSK945162
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 47025; Mature: 46894

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRRALALAALALAVSPALAAQERPTIVVGSPDFRPLPIAVAAFQGEGDAGAAATQTAE
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHH
VVRADLVLSGLFDVLDPRGFLADPSEGFAAPSIRFARWADVGADGLAKARVRRGPAGLEG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCC
ELHLYEVRAGREVLVKLLRVDGADARSLAHRMADEIVRYYTREPGIFATRIAAIRRGRGT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
WELVTQDMDGGNQQVLLSERSILMSPAWRPDGREILVTSYRSGRPELWAYRFSDRAFRPL
EEEEEECCCCCCCEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCHH
GRHRNAFGGVYSPDGSRIAFTVSEGNVTDLWVMSADGAGARKLTSDPAIDVSPTWSPDGR
HCCCCCCCCEECCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEECCCCCCCCCC
RIAFVSDRSGTPQIYVMGADGSGARRLTFQGNYNQTPQWSPRGDLIAFTARDERKVFDVF
EEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEE
VVSPDSGAINRITQDQGRTNEEPSWAPNGRLMIFRTDRNGGIQLVVSDARGDRQTPVTSG
EECCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCC
KTDLAAPAWGPLAP
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TVRRALALAALALAVSPALAAQERPTIVVGSPDFRPLPIAVAAFQGEGDAGAAATQTAE
CHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHH
VVRADLVLSGLFDVLDPRGFLADPSEGFAAPSIRFARWADVGADGLAKARVRRGPAGLEG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCC
ELHLYEVRAGREVLVKLLRVDGADARSLAHRMADEIVRYYTREPGIFATRIAAIRRGRGT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
WELVTQDMDGGNQQVLLSERSILMSPAWRPDGREILVTSYRSGRPELWAYRFSDRAFRPL
EEEEEECCCCCCCEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCHH
GRHRNAFGGVYSPDGSRIAFTVSEGNVTDLWVMSADGAGARKLTSDPAIDVSPTWSPDGR
HCCCCCCCCEECCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEECCCCCCCCCC
RIAFVSDRSGTPQIYVMGADGSGARRLTFQGNYNQTPQWSPRGDLIAFTARDERKVFDVF
EEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEE
VVSPDSGAINRITQDQGRTNEEPSWAPNGRLMIFRTDRNGGIQLVVSDARGDRQTPVTSG
EECCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCC
KTDLAAPAWGPLAP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA