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The map label for this gene is glnG [H]

Identifier: 220915717

GI number: 220915717

Start: 673062

End: 674930

Strand: Direct

Name: glnG [H]

Synonym: A2cp1_0598

Alternate gene names: 220915717

Gene position: 673062-674930 (Clockwise)

Preceding gene: 220915716

Following gene: 220915718

Centisome position: 13.38

GC content: 74.8

Gene sequence:

>1869_bases
ATGGCGAACGGGCGGATCCTGGTCGTCGACGACGAGCGGTTCTTCCAGGAGCTGTTCCGCGACGTGCTGACCGGCGCCGG
CCACGCGGTCCGCACCTCGTCCTCGGCGGAGGAGGCGCTGGAGGTGCTCGCCGCCGAGCGGGTGGATCTCCTGCTCACCG
ACATGGTGATGCCGGGCCTCGACGGCCTGGGGCTCATCCGCGAGGCGCGGCGGCGCGACCCGGAGCTGGAGGTGGTGGCG
GTCACCGGGCGCGAGGACGTGAAGCTCGCGGTCGAGTCGATGAAGGCCGGCTGCTCCGACTTCCTGACGAAGCCGCTCGA
GCCCGGCGAGCTGCTCCGCGTGGCGGAGCGGGTGCTCGGGCGGGTGCGGCTCCGCCGCGAGCACAGCCAGCTCCTCACCG
AGAACCTCGAGTTCGTGAAGGCCCAGGCGCTCTACCGCCAGGGCCTGCAGATCCTCGCCACGCTCGACGGCGAGCGGCTG
GTGGACCTGGCGCTCTCCGTGCTGGCGCGCACCACCGACGCCCAGGGCGCGGCGCTGTGGGTGGCCGACGAGAAGGGTCA
GCTCGCGCTGCGCGGCTATCGCGGCCTCGTCGATCGGGCGGCGCTGCCGGTCCGGGTGGACCCGCGCGATCCCGCGTTCG
CCGACGCGCTGCGCGGCGGCGCGCCGTTCCTGGCGCCGGACGGCTGGGGCGGCGAGGCGTTCCTGGTGCCGCTCGTGGCC
GACGACGAGCCGCTCGGCCTGGCGCTGCTCTCCGACCGCGCCCGCGGGCGCTTCGGCCGTGACGAGCACGCGGCCGCGCT
GGTGGTGGCCGACTTCACCGCCATCGCGGTGAAGAACGCGCGCCGCTTCCAGGCGGTGGAGCGGGTGGGGCTGCGCGACC
GCGAGACCGGCGCGTACAACCTCTCCTACTTCGTCGACTACGCCGGCAAGGAGTTCTACAAGGCGCGCCGCTACGGCCGG
GCGTTCTCGCTGGTGGTGCTCTCGGTGGACAACGTCGAGCAGCTCCGCAAGGAGGCGGGGCGCGAGCCGTTCCGGCGGCT
GATGCGCGACCTGGTGGCGGCGGTGGGGCGGGCGGTCCGCGACGCGGACATCCTCGCGAAGGTCTCGGAGAGCGAGTACT
ACGTGCTGCTCCCCGAGACCGACTACTTCGGCGCGCTCATGTTCGTGCGCCGCGCCACCGAGGAGGTCCGGCGCGAGGCG
TCGGTCCGGACCATGGAGGAGCGCGTGCCGGTGCTCCTCTCGATCGGCGCCGCCAGCTTCCCGAAGGACGGGGAGGACTT
CGACGAGCTGCTCCACTGGGCGCGCGACCGCGTGCAGGACCAGCGCGGGTCGCTGCTGCGCCGCCTGCACCTCGGCGACC
TCGAGCCGGGCGCGTTCTGGGAGCTGACCGACGTGCTGCTCTCCGGCTCCGCGCGCCTGCCGGACAGCTCGGCCAGCGCG
CGGCTGCAGCCGGACGCGGAGCTGTTCGCGGCCGCGCAGCGCGAGGCGGCCCGCGAGATCGCGCGCGACCCGCGCGCCCG
TGGCCTGCTCTACGTGGGGCTGCGCACCGCGCTCGCCGCGTCGCCGGTCCGGGCGGCGCTGCCCCCCGGGGACCCGGCCG
CGCGCGCCGGCGATCCCTCCGCCCGCGTGTACCTGCTCGGCCCGCGCGGCACCGACGCCGAGGCGGCGCCGCACCCGCTC
GTCACCGAGGTGTACGTGGACGGCGACCAGCGCTTCGCCGGCCACGAGTTCCTGCTGTTCCTCTCCGAGCGCTCCGCGTA
CGCGCTGCTCGCCGGCCCGGACGGCCGGACCTTCCACACCTCCGACATGCCGCTGGTGGACGCGCTGGTCTCGAAGCTGC
AGACCGCGTACGACCTCCAGCCGATCTGA

Upstream 100 bases:

>100_bases
GCCGGGCGGCGCGCTCGCCATCACCGTGGCCCCCGATCTCGTCGGCGTCACCATGCGCGGCCCGGCCGAGCGCGCCTTCA
CCGGCGAGACGGACCTCTAG

Downstream 100 bases:

>100_bases
GCGACCGCGCGAATGGCCACCGTCCGCAAGATCCTCGTCGCCGACCCCGACCCGGCCACCGTTCGGGCGCTGGCGCCGGT
GCTCCGCCAGCGCGGCTACC

Product: response regulator receiver modulated GAF sensor protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MANGRILVVDDERFFQELFRDVLTGAGHAVRTSSSAEEALEVLAAERVDLLLTDMVMPGLDGLGLIREARRRDPELEVVA
VTGREDVKLAVESMKAGCSDFLTKPLEPGELLRVAERVLGRVRLRREHSQLLTENLEFVKAQALYRQGLQILATLDGERL
VDLALSVLARTTDAQGAALWVADEKGQLALRGYRGLVDRAALPVRVDPRDPAFADALRGGAPFLAPDGWGGEAFLVPLVA
DDEPLGLALLSDRARGRFGRDEHAAALVVADFTAIAVKNARRFQAVERVGLRDRETGAYNLSYFVDYAGKEFYKARRYGR
AFSLVVLSVDNVEQLRKEAGREPFRRLMRDLVAAVGRAVRDADILAKVSESEYYVLLPETDYFGALMFVRRATEEVRREA
SVRTMEERVPVLLSIGAASFPKDGEDFDELLHWARDRVQDQRGSLLRRLHLGDLEPGAFWELTDVLLSGSARLPDSSASA
RLQPDAELFAAAQREAAREIARDPRARGLLYVGLRTALAASPVRAALPPGDPAARAGDPSARVYLLGPRGTDAEAAPHPL
VTEVYVDGDQRFAGHEFLLFLSERSAYALLAGPDGRTFHTSDMPLVDALVSKLQTAYDLQPI

Sequences:

>Translated_622_residues
MANGRILVVDDERFFQELFRDVLTGAGHAVRTSSSAEEALEVLAAERVDLLLTDMVMPGLDGLGLIREARRRDPELEVVA
VTGREDVKLAVESMKAGCSDFLTKPLEPGELLRVAERVLGRVRLRREHSQLLTENLEFVKAQALYRQGLQILATLDGERL
VDLALSVLARTTDAQGAALWVADEKGQLALRGYRGLVDRAALPVRVDPRDPAFADALRGGAPFLAPDGWGGEAFLVPLVA
DDEPLGLALLSDRARGRFGRDEHAAALVVADFTAIAVKNARRFQAVERVGLRDRETGAYNLSYFVDYAGKEFYKARRYGR
AFSLVVLSVDNVEQLRKEAGREPFRRLMRDLVAAVGRAVRDADILAKVSESEYYVLLPETDYFGALMFVRRATEEVRREA
SVRTMEERVPVLLSIGAASFPKDGEDFDELLHWARDRVQDQRGSLLRRLHLGDLEPGAFWELTDVLLSGSARLPDSSASA
RLQPDAELFAAAQREAAREIARDPRARGLLYVGLRTALAASPVRAALPPGDPAARAGDPSARVYLLGPRGTDAEAAPHPL
VTEVYVDGDQRFAGHEFLLFLSERSAYALLAGPDGRTFHTSDMPLVDALVSKLQTAYDLQPI
>Mature_621_residues
ANGRILVVDDERFFQELFRDVLTGAGHAVRTSSSAEEALEVLAAERVDLLLTDMVMPGLDGLGLIREARRRDPELEVVAV
TGREDVKLAVESMKAGCSDFLTKPLEPGELLRVAERVLGRVRLRREHSQLLTENLEFVKAQALYRQGLQILATLDGERLV
DLALSVLARTTDAQGAALWVADEKGQLALRGYRGLVDRAALPVRVDPRDPAFADALRGGAPFLAPDGWGGEAFLVPLVAD
DEPLGLALLSDRARGRFGRDEHAAALVVADFTAIAVKNARRFQAVERVGLRDRETGAYNLSYFVDYAGKEFYKARRYGRA
FSLVVLSVDNVEQLRKEAGREPFRRLMRDLVAAVGRAVRDADILAKVSESEYYVLLPETDYFGALMFVRRATEEVRREAS
VRTMEERVPVLLSIGAASFPKDGEDFDELLHWARDRVQDQRGSLLRRLHLGDLEPGAFWELTDVLLSGSARLPDSSASAR
LQPDAELFAAAQREAAREIARDPRARGLLYVGLRTALAASPVRAALPPGDPAARAGDPSARVYLLGPRGTDAEAAPHPLV
TEVYVDGDQRFAGHEFLLFLSERSAYALLAGPDGRTFHTSDMPLVDALVSKLQTAYDLQPI

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=33.8582677165354, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=33.0578512396694, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=131, Percent_Identity=32.0610687022901, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=28.4615384615385, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788394, Length=126, Percent_Identity=32.5396825396825, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1787375, Length=108, Percent_Identity=34.2592592592593, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1787229, Length=128, Percent_Identity=32.8125, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788905, Length=116, Percent_Identity=32.7586206896552, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 68384; Mature: 68252

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGRILVVDDERFFQELFRDVLTGAGHAVRTSSSAEEALEVLAAERVDLLLTDMVMPGL
CCCCEEEEEECHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DGLGLIREARRRDPELEVVAVTGREDVKLAVESMKAGCSDFLTKPLEPGELLRVAERVLG
CCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHH
RVRLRREHSQLLTENLEFVKAQALYRQGLQILATLDGERLVDLALSVLARTTDAQGAALW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEE
VADEKGQLALRGYRGLVDRAALPVRVDPRDPAFADALRGGAPFLAPDGWGGEAFLVPLVA
EECCCCCEEEHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
DDEPLGLALLSDRARGRFGRDEHAAALVVADFTAIAVKNARRFQAVERVGLRDRETGAYN
CCCCCCEEEECCHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LSYFVDYAGKEFYKARRYGRAFSLVVLSVDNVEQLRKEAGREPFRRLMRDLVAAVGRAVR
EEEEEEHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DADILAKVSESEYYVLLPETDYFGALMFVRRATEEVRREASVRTMEERVPVLLSIGAASF
HHHHHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
PKDGEDFDELLHWARDRVQDQRGSLLRRLHLGDLEPGAFWELTDVLLSGSARLPDSSASA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
RLQPDAELFAAAQREAAREIARDPRARGLLYVGLRTALAASPVRAALPPGDPAARAGDPS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCC
ARVYLLGPRGTDAEAAPHPLVTEVYVDGDQRFAGHEFLLFLSERSAYALLAGPDGRTFHT
CEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEC
SDMPLVDALVSKLQTAYDLQPI
CCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANGRILVVDDERFFQELFRDVLTGAGHAVRTSSSAEEALEVLAAERVDLLLTDMVMPGL
CCCEEEEEECHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DGLGLIREARRRDPELEVVAVTGREDVKLAVESMKAGCSDFLTKPLEPGELLRVAERVLG
CCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHH
RVRLRREHSQLLTENLEFVKAQALYRQGLQILATLDGERLVDLALSVLARTTDAQGAALW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEE
VADEKGQLALRGYRGLVDRAALPVRVDPRDPAFADALRGGAPFLAPDGWGGEAFLVPLVA
EECCCCCEEEHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
DDEPLGLALLSDRARGRFGRDEHAAALVVADFTAIAVKNARRFQAVERVGLRDRETGAYN
CCCCCCEEEECCHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LSYFVDYAGKEFYKARRYGRAFSLVVLSVDNVEQLRKEAGREPFRRLMRDLVAAVGRAVR
EEEEEEHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DADILAKVSESEYYVLLPETDYFGALMFVRRATEEVRREASVRTMEERVPVLLSIGAASF
HHHHHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
PKDGEDFDELLHWARDRVQDQRGSLLRRLHLGDLEPGAFWELTDVLLSGSARLPDSSASA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
RLQPDAELFAAAQREAAREIARDPRARGLLYVGLRTALAASPVRAALPPGDPAARAGDPS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCC
ARVYLLGPRGTDAEAAPHPLVTEVYVDGDQRFAGHEFLLFLSERSAYALLAGPDGRTFHT
CEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEC
SDMPLVDALVSKLQTAYDLQPI
CCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]